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集團給食所 및 家庭의 廚房에서 使用하는 행주의 衛生學的 處理에 관한 硏究
李雄洙,崔錫南 충주대 2003 産業科學論文集 Vol.11 No.-
This study was carried out to find out the method on hygienic treatment of dishcloth used at the cookroom of mass meal service center and household in order to prevent food poisoning and oral infectious disease. This research was examined with three methods(washing, boiling and chlorine solution treatment) based on materials for the questionnaire by 28 persons who engaged in mass meal service center and telephone interview by 100 housewives. The indicator bacteria on the fecal contamination in food hygiene such as Escherichia coli, Staphylococcus aureus, Brochothrix thermosphacta, Salmonella typhimurium, vibrio parahae-molyticus and pseudomonas aeruginosa, and Shigella sonnei, one of oral epidemic bacteria, were used in this study. The results of this study related to food hygienic and micro-biological tests were the following : 1) By washing treatment, the indicator bacteria and Shigella sonnei were detected less 1/2 to 1/4 than initial viable cell counts. 2) After boiling treatment for 10min, E. coli, Pseudo. aeruginosa and Brocho. thermo-sphacta were found 63-340 CFU/㎖, and Staphy. aureus and Sal. typhimurium were detected 1-2CFU/℃, but Vibrio parahaemolyticus and Shigella sonnei not detected. 3) After boiling treatment for 20min, E. coli, Brocho. thermosphacta and Pseudo. aeru-ginosa were detected 4-130CFU/℃, but Staphy. aureus, Sal. typhimurium, Vibrio par-ahaemolyticus and Shigella sonnei died out completely. 4) After boiling treatment for 30min, only Pseudo. aeruginosa was found, and the other bacteria were not detected. 5) In case of soaking treatment in chlorine solution, all tested bacteria were detected to 1-2500 CFU/℃ in 80ppm solution. In 160ppm chlorine solution, Brocho. thermosphacta, Sal. typhimurium, Vibrio parahaemolyticus, Pseudo. aeruginosa and Shigella sonnei were existed to 2-32CFU/℃, but E. coli and Staphy. aureus were died out. In 320ppm chlorine solution, only Pseudo. aeruginosa was found to 13CFU/℃, and the other bacteria were not detected. In 640ppm chlorine solution, all tested bacteria were not detected. In conclusion, this study suggested that boiling over 30min and soaking in 640ppm chlorine solution are very good methods as the hygienic treatment of dishcloth contaminated with food poisoning and oral infectious disease bacteria.
李雄洙 忠州大學校 1994 한국교통대학교 논문집 Vol.29 No.2
The purpose of this study was to determine if fumonisin B₁(FB₁), a promoter for hepa tocarcinogenesis in rat, induces lipid peroxidation and alters the concentrations of antioxidant systems in rat liver cell. The hepatocytes were isolated from male Harlan Sprague-Dawley rat by using aseptic procedures, and treated with varying concentrations of FB₁(1μM,10μM and 50μM). The levels of malondialdehyde(MDA), conjugated diene(CD), vitamin A(V-A), vitamin E(V-E),γ-tocopheryl quinone(γ-TQ), vitaminC(V-C), glutathione(GSH), total lipids(TL), and protein were determined to assess oxidative damage and antioxidant status on the hepatic cell. FB₁ increased the levels of MDA and CD, oxidative products of cellular lipid. And FB₁ decreased the levels of cellular antioxdants such as V-E, and V-A, and V-C, but increased GSH level. This study shows that the adiministration of FB₁, a mycotoxin produced by Fusarium moniliforme, did increase indices of lipid peroxidation, but that cellular antioxidant defences were inhibited by FB₁.
이웅수 忠州大學校 2003 한국교통대학교 논문집 Vol.38 No.2
In this research, whether dietary vitamin E has a direct functional effect on the production of reactive oxygen species(ROS) in mitchondria, and whether dietary vitamin E influence the formation of free iron in rat tissues were determined. Additionally, if altered iron release is linked to the degree of peroxidative damage was determined. The results clearly showed that dietary vitamin E markedly reduced hydrogen peroxide production in the mitochondria of liver and skeletal muscle of both male and female rats in a dose-dependent manner. Also, the data showed that deprivation of vitamin E resulted in an increase of free iron in the skeletal muscle and spleen of male rats and liver, skeletal muscle, heart, kidney, and spleen of female rats. Supplementation with vitamin E significantly decreased the levels of free iron in the skeletal muscle of both male and female rats. Also, the levels of free iron were significantly inversely related to the concentration of supplemented vitamin E in the skeletal muscle, kidney, and spleen of male rats and liver, skeletal muscle, heart, kidney, and spleen of female rats. Deprivation of vitamin E caused a significant increase in the levels of thiobarbituric acid reactants(TBARS) in the liver, skeletal muscle, heart, kidney, and spleen of both male and female rats. Vitamin E does-dependently decreased TBARS in the liver and kidney of male rats and liver, skeletal muscle, and kidney of female rats. The results obtained from this research indicate that vitamin E regulates mitochondrial generation of ROS and attenuates the release of iron from its protein complexes. The results also suggest that the overprodution of mitochondrial ROS is the first event leading to tissue damage observed in vitamin E-deficiency syndromes and that formation of free iron is the first event of oxidative tissue damage.
Aflatoxin B₁의 간발암성에 대한 nivalenol의 영향
李雄洙 충주대 산업과학기술연구소 1999 産業科學論文集 Vol.7 No.2
This research was carried out to investigate the toxicological effect of long-term intake of nivalenol(NIV), a trichothecene mycotoxin produced by Fusarium nivale Fn 2B, and the combined toxicity of NIV and aflatoxin Bl(Af-Bl), a potent hepatocarcinogenic mycotoxin, in mice. In male mice, liver tumors developed to all Af-Bl treated groups, and most of them were hepatocellular carcinomas. The incidence of Af-Bl induced liver tumors was not altered by feeding of NIV containing diet. In female mice, however, the incidences of liver tumors were 31%, 20% and 0% in Af-Bl alone group, Af-Bl plus NIV 6ppm group and Af-Bl plus NIV 12ppm group, respectively. NIV dose dependent pattern on hepatocarcinogenesis by Af-Bl showed a decreasing tendency. Incidence of liver tumors in NIV 12ppm group was significantly lower than in Af-Bl alone group. Most of liver tumors in female mice were hepatocellular adenomas, and the malignancy of Af-Bl induced liver tumors was lower than that of male mice. The remarkable change of biochemical factor on serum was not observed by administration of NIV. In these results, NIV has no tumorous effect in mice, and increased significantly life span. And the inhibition effect of NIV on Af-Bl induced hepatocarcinogenesis was suggested in mice.