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이완,Hyo-Im Choi,Mi-Jin Kim,박승윤 생화학분자생물학회 2008 Experimental and molecular medicine Vol.40 No.1
The mutation and reduction of mitochondrial DNA (mtDNA) have been suggested as factors in the car-cinogenesis. However, whether the depletion of mtDNA induces multidrug resistance in cancer cells has not been fuly investigated. To elucidate the asso-ciation of cellular mtDNA content and drug resistance, a marked decrease in cellular mtDNA and ATP content, concomitant with a lack of mRNAs encoded by mtDNA. The mtDNA-depleted cels showed a decreased sensi-tivity and accumulation of anti-cancer drugs, sugest-ing that mtDNA depletion could develop multidrug re-sistance (MDR) phenotype in HCT-8 cells. We found that the expression level of MDR1 mRNA and its trans-lated product P-glycoprotein was increased in the sensitivity and accumulation of anti-cancer drug in the mtDNA-depleted cells might be due to a substantial in-crease in the expression of P-glycoprotein. Further-more, increased expression of MDR1 mRNA and P-gly-coprotein was due to an increase of mRNA stability rather than transcriptional activation. Taken together, these results indicate that mtDNA depletion can induce an increased P-glycoprotein expression via an in-crease of mRNA stability and suggest that the mtDNA depletion in cancer cells plays an important role in the induction of MDR phenotype.
생체외 L-carnitine과 Acetylcarnitine의 정자지표 개선 효과
이완,박남철,Lee, Wan,Park, Nam-Cheol 대한생식의학회 2002 Clinical and Experimental Reproductive Medicine Vol.29 No.2
Objectives: To assess the scavenging effect of carnitine derivatives on oxidative damage to sperm during sperm processing, cryopreservation and thawing. Materials and Methods: Fresh semen samples from 20 normal healthy volunteers were collected by masturbation after at least 48 hours abstinence. After liquefaction of semen samples at room temperature, the specimens were diluted with sperm wash media (Ham's F-10, Life technologics) to a uniform density of $20{\times}10^6/ml$. L-carnitine or acetylcarnitine were added with various concentration of $0{\mu}M$, $10{\mu}M$, $30{\mu}M$ in semen sample or cryoprotectant. All specimens were cryopreservated at $-196^{circ}C$ $LN_2$ for 3 days. Sperm motility, vitality, fertilizing capacity, reactive oxygen species formation and the level of lipid peroxidation were analyzed by computer assisted semen analyzer, eosin-nigrosin stain, hypoosmotic swelling test, chemiluminescence and thiobarbituric acid method, respectively, during sperm processing, cryopreservation and thawing. Results: The sperm motility was only increased in proportion to the concentration of acetylcarnitine with no statistical significance (p>0.05). The sperm vitality was also significantly improved in proportion to the concentration of acetylcarnitine with statistical significance (p<0.05). The sperm fertilizing capacity was significantly increased in proportion to the concentration of L-carnitine and acetylcarnitine and reactive oxygen species generation and lipid peroxidation were significantly decreased with same fashion (p<0.05). On comparison of effects between L-carnitine and acetylcarnitine, acetylcarnitine was superior to L-carnitine on the improvement of sperm motility and vitality as well as the suppression of reactive oxygen species generation and lipid peroxidation. Conclusions: These results suggest that carnitine derivatives have a scavenging effect against oxidative damages during sperm processing, cryopreservation and thawing. Therefore, carnitine derivatives may be useful as an oral antioxidant in patients with male infertility due to increased ROS generation.
웹 서비스 코리오그래피의 상호운용성 지원을 위한 XML 언어 개발
이완,정범석,박종헌 한국경영정보학회 2007 한국경영정보학회 학술대회논문집 Vol.2007 No.-
웹 서비스가 현재 산업에서 주요한 분산 컴퓨팅 기술로 대두되면서, 그에 따른 연구가 활발히 이루어지고 있다. 특히, 비즈니스 프로세스 관점에서 BPEL4WS (Business Process Execution Language for Web Services)와 같은 오케스트레이션의 연구가 많이 이루어지고 있다. 또한, 웹 서비스의 주요 구성 요소중에 하나인 코리오크래피에 대한 부분은 W3C (World Wide Web Consortium)에서 현재 후보 권고안 수준인 WS-CDL (Web Services Choreography Description Language) 관련 연구가 이루어지고 있다. 이런 가운데 DERI (Digital Enterprise Research Institute)에서 WSMX (Web Services Execution Environment) 기반의 시맨틱 웹 서비스 분야의 코리오 그래피 연구를 시작하였고, 코리오그래피 불일치 (Mismatch)를 개념적으로 해결하는 방안인 프로세스 미디에이션 (Process Mediation)의 연구가 있었다. 이러한 연구들을 바탕으로 서로 다른 WS-CDL 상에서 특정 참여자가 코리오그래피 미디에이션을 하기위해서 개발된 XML 언어 WS-CML (Web Services Choreography Mediation Language)을 본 논문에서 제안한다.