피하 주머니 이식술을 이용한 수부 무지 첨부 재건술 - 2예 보고 -

이영근,문영재,이준모,Lee, Young-Keun,Moon, Young-Jae,Lee, Jun-Mo 대한미세수술학회 2011 Archives of reconstructive microsurgery Vol.20 No.2

Purpose: The subcutaneous pocket graft of the thumb tip amputation across or proximal to the lunula is chosen in case of impossible microvascular anastomosis and in patient who strongly desired to preserve the thumb tip after failed replantation. Materials and Methods: Two patients who underwent a subcutaneous pocket graft for a thumb tip reconstruction between August 2008 and November 2009 were reviewed retrospectively. They were all males with a mean age at the time of surgery of 48 years and had sustained complete thumb tip amputations across or proximal to the lunula. In one case, the microsurgical replantation was not feasible and the other one revealed arterial insufficiency at the 7th day after microsurgical replantation. Results: Authors had experienced 2 cases of flaps which survived completely. The results of sensibility was good, the range of motion at interphalangeal joint and tip to tip pinch was acceptable and color mismatch and loss of thumb finger nail was unacceptable after more than 1 year follow up with conventional successful thumb tip replantation. Conclusion: The subcutaneous pocket graft could be chosen in thumb tip amputation in case of impossible microvascular anastomosis as well as who strongly desires to preserve thumb tip after failed replantation.

사탕수수 찌꺼기를 이용한 여름느타리 발효부산물의 생물활성

이영근,장화영,김원록,김진규,김재성 ( Young Keun Lee,Hwa Hyoung Chang,Won Rok Kim,Jin Kyu Kim,Jae Sung Kim ) 한국환경농학회 1998 한국환경농학회지 Vol.17 No.1

In order to evaluate the biological activities of some fractions from the fungal(Pleurotus sajorcaju) fermentation products of sugar cane bagasses, the antimutagenicity, the glyceollin elicitor activity on soybean and the effect on the stem elongation in pea were observed. The alcohol extract fraction and DMSO soluble fraction had excellent antimutagenicity even though it is weaker than that of the extracts from the fruiting bodies. All of the extracts had the ability to elicit glyceollins in soybean cotyledons and these extracts could be helpful for plants to protect themselves from pathogenic contaminations. IAA and the extracts had shown synergistic effects on pea stem elongation in all experimental groups positively detemlined.

수근부 자해열상의 수술적 치료

이영근,박찬일,이준모,Lee, Young-Keun,Park, Chan-Il,Lee, Jun-Mo 대한미세수술학회 2012 Archives of reconstructive microsurgery Vol.21 No.1

Purpose: To report clinical appearances and the outcomes of patients treated for wrist laceration with suicidal intent. Materials and Methods: Between March 2005 and February 2008, patients with wrist laceration were treated at our center. Among them, 14 patients with suicidal intent were reviewed retrospectively. They were 8 females and 6 males with a mean age of 33 years at the time of treatment. The side of injured wrist, injured structures, suicidal instrument, the time of injury, a suicidal motive, drug or alcohol abuse or not, indication of combined psychiatric disease, the duration of postoperative treatment, and the results of treatment were analyzed. Results: The injured wrist was the left one in 13 cases (93%), and knife wounds in 11 cases (79%) was the most frequent mechanism of injury. An average of 4.6 structures were injured including 3.5 tendons, 0.57 nerves and 0.5 arteries. The most frequently injured structures were the palmaris longus (71%), FCU (50%), FCR (43%), FDS 3 (36%), FDS 2, median nerve, and ulnar artery (each 29%). Simultaneous injury of both median and ulnar nerves occurred in 3 cases (21%), and simultaneous injury of both radial and ulnar artery occurred in 1 case (7%). The injury time was the night in 12 cases (86%), and the most frequent suicidal motive was human relations such as lover or spouse in 12 cases (86%). In 9 cases (64%), the injured were in a drunken state, 3 cases (21%) had been treated for a psychiatric disease at the time of the injury. The durations of postoperative treatment were an average of 5 days of admission and 1 day follow up. Only 1 case results could be evaluated. Conclusions: Patient's poor compliance in wrist laceration with suicidal intent should be expected and these characteristics may affect the management plan.

돼지여포의 퇴화과정 중 여포액 내 스테로이드 호르몬 양의 변화

이영근,윤용달,Lee, Young-Keun,Yoon, Yong-Dal 대한생식의학회 1985 Clinical and Experimental Reproductive Medicine Vol.12 No.1

In order to study the mechanism of follicular atresia, the follicles of the porcine ovary were isolated according to the presence or absence of the corpus luteum and their size, and then classified to the normal? or atretic?follicle on the morphological observation such as the transparency, the vascularization of follicle, the nuclear phase of oocyte, and the homogeneity of the granulosa cell layer. The viability of granulosa cells was examined. The concentrations of progesterone ($P_4$), testosterone (T), and estradiol-17 beta ($E_2$) in each follicular fluid were estimated by the radioimmunoassay. The viability of granulosa cells in the atretic follicle was much lower than that of the normal one. The concentration of each steroid hormone increased as the follicular size was increased, was not different in quantity between the normal- and the atretic follicle of which diameter was below 3mm, and were much higher in the atretic follicle than those in the normal one of which diameter was above 7mm. The ratio of the concentration of E2 to T in the large atretic follicle valued higher than that in the normal one, but smaller in the small and medium atretic follicle than that in the normal one. The present study suggests that the mechanism of atresia of the large follicle may be different from that of the small and the medium follicle and that the amount of steroid hormones regarded as the one of the criteria for the atretic follicles.

수지 재건시 불충분한 혈행상태의 수혜부에 시행한 동맥화 정맥 유리 피판술

이영근,박기태,이준모,박혁,Lee, Young-Keun,Park, Ki-Tae,Lee, Jun-Mo,Park, Hyuk 대한미세수술학회 2012 Archives of reconstructive microsurgery Vol.21 No.2

Purpose: Arterialized venous flap is useful for reconstruction of the traumatic soft tissue defect in fingers, but insufficient circulation of the traumatic fingers makes surgeons annoying to use the flap. We have grafted flaps in 7 fingers with insufficient vascular bed hoping to expanded the category of the flap. Materials and Methods: Arterialized venous flap have transplanted in 7 fingers from March 2008 through February 2010 and followed up for 4 to 16 months(average 7.2 months). They were all male with a mean age at the time of surgery was 33. The main injury was crushing in 4 degloving, contact burn and saw injury was I respectively. Time interval from injury to flap transplantation was average 3.1. weeks(3 days to 6 weeks). Designed flap size ranges from $8cm{\times}3.5cm$to $4cm{\times}3cm$. Vessel type of flap was one artery with two veins were 5 cases and one artey with one vein 2. Flap type was cutaneous in 3, tendocutaneous 2, neurotendocutaneous 1 and neurocutaneous 1. The circulation state of recipient site was avascular in 2 cases, insufficiency 3 and tip avascular 2. Results: Arterialized venous flap was complete survived in 2 cases, partial necrosis(less than 10%) 3 and failed in 2. Conclusion: An arterialized venous free flap could be a useful procedure for reconstruction in soft tissue or combined defect of the finger despite an avascular or insufficient vascular beds if the recipient beds were free from infection.

방사선을 이용한 느타리 ( Pleurotus ostreatus ) 의 섬유소 분해 변이주 선발

이영근,장화형,김원록,이창주,김진규 ( Young Keun Lee,Hwa Hyoung Chang,Won Rok Kim,Chang Joo Lee,Jin Kyu Kim ) 한국환경생물학회 1998 환경생물 : 환경생물학회지 Vol.16 No.2

To isolate the cellulolytic variants, the basidiospores of Pleurotus ostreatus were irradiated at the dose of 1Gy∼20kGy of gamma-ray. After irradiation the germination and survival rates of basidiospores were observed and the activity of extracellular enzyme was determined by means of MUF-substrate method. The 45 variants showed various levels of mycelial growth rate and cellulolytic activity on solid media and vari ous levels of activities of the extracellular enzymes. We have isolated highly cellulolytic variants. 1.5KG-2 and 2KG-2. among the above variants. It is suggested that the isolated 2 variants could be applied usefully to reuse and/or recycle of the cellulosic bioresources.

유리 동맥화 정맥 피판을 이용한 수지 연부조직 결손의 재건

이영근,우상현,이준모,안희찬,천호준,Lee, Young-Keun,Woo, Sang-Hyun,Lee, Jun-Mo,Ahn, Hee-Chan,Cheon, Ho-Jun 대한미세수술학회 2010 Archives of reconstructive microsurgery Vol.19 No.1

Purpose: To report the clinical results of the use of arterialized venous free flaps in reconstruction in soft tissue defects of the finger and to extend indications for the use of such flaps based on the clinical experiences of the authors. Materials and Methods: Eighteen patients who underwent arterialized venous free flaps for finger reconstruction, between May 2007 and July 2009 were reviewed retrospectively. The mean flap size was 4.7${\times}3.2$ cm. The donor site was the ipsilateral volar aspect of the distal forearm in all cases. There were 8 cases of venous skin flaps, 5 cases of neurocutaneous flaps, 4 cases of tendocutaneous flaps, 1 case of innervated tendocutaneous flap. The vascuality of recipient beds was good except in 4 cases (partial devascuality in 2, more than 50% avascuality (bone cement) in 2). Results: All flaps were survived. The mean number of included veins was 2.27 per flap. Mean static two-point discrimination was 10.5 mm in neurocutaneous flaps. In 3 of 5 cases where tendocutaneous flaps were used, active ROM at the PIP joint was 60 degrees, 30 degrees at the DIP joint and 40 degrees at the IP joint of thumb. There were no specific complications except partial necrosis in 3 cases. Conclusions: An arterialized venous free flap is a useful procedure for single-stage reconstruction in soft tissue or combined defect of the finger; we consider that this technique could be applied to fingers despite avascular recipient beds if the periphery of recipient bed vascularity is good.

염생식물 나문재(Suaeda asparagoides M<SUB>IQ</SUB>)의 이화학적 성분 조성

이영근(Young-Keun Lee),이윤신(Yoon-Shin Lee),정은경(Eun Kyung Jung) 동아시아식생활학회 2010 동아시아식생활학회지 Vol.20 No.3

To obtain basic information on the utilization of Suaeda (S.) asparagoides M<SUB>IQ</SUB> as a raw material in food, it's chemical components of were investigated. S. asparagoides M<SUB>IQ</SUB> from the Hwaseong-si in Korea was used after drying and grinding with powder. The total dietary fiber, insoluble dietary fiber and soluble dietary fiber contents of S. asparagoides M<SUB>IQ</SUB> were found to be 39.41%, 37.63% and 1.78%, respectively. Macromineral components of S. asparagoides M<SUB>IQ</SUB> were Na (7.6%), K (2.4%) and Mg (0.6%). High contents of microminerals such as Cu, Mn and Zn were also found. Lysine and methionine which are essential amino acids were detected with the value of 215 ㎎/100 g and 23.2 ㎎/100 g, respectively. S. asparagoides M<SUB>IQ</SUB> that was harvested in May was higher in crude protein than that harvested in September but the crude fat content of that which was harvested in September was twice as high as that harvested in May.

RAW 264.7 세포에서 지모(知母) 80% 에탄올 추출물의 항염증 효과

이영근 ( Young Keun Lee ),김청택 ( Cheong Taek Kim ),최학주 ( Hak Joo Choi ) 대한본초학회 2017 大韓本草學會誌 Vol.32 No.3

Objective : According to recent studies, Anemarrhenae Rhizoma has anti-inflammatory activities of DW extract, but it hasn`t not yet conducted to evaluate inflammatory factors about 80% ethanol extract. Therefore, The aim of this study is to investigate the various effects of individual or combined 80% ethanol extract of Anemarrhenae Rhizoma on cell viability and various anti-inflammatory factors. Methods : Anemarrhenae Rhizoma extract was prepared with 80% ethanol. MTT assay, ELISA, and Luminex were performed in LPS-activated RAW 264.7 cell line to measure cytotoxicity, Nitric oxide (NO), cyclooxygenase-2 (COX-2), prostaglandin E2 (PGE₂), Leukotriene B4 (LTB₄), and cytokines (IL-1β, IL-6, and TNF-α), respectively. Results : At concentration of 200 ㎍/㎖ Anemarrhenae Rhizoma extract, cytotoxicity was observed in RAW 264.7 cells. However, at concentration less than 100 ㎍/㎖ of Anemarrhenae Rhizoma, cytotoxicity was not observed in RAW 264.7 cells. All concentration of Anemarrhenae Rhizoma extract showed no difference of NO, and IL-1β level in RAW 264.7 cells compared with control group. In contrast, at concentration of 100 ㎍/㎖ Anemarrhenae Rhizoma extract significantly inhibited LPS-induced production of COX-2, PGE₂, and LTB₄ level in RAW 264.7 cells. In addition, the production of proinflammatory cytokines (IL-6, TNF-α) in LPS-induced RAW 264.7 cells was significantly decreased at concentration of all or 10, and 100 ㎍/㎖, respectively. Conclusion : These findings demonstrate that Anemarrhenae Rhizoma has inhibitory effect on inflammatory mediators in LPS-activated RAW 264.7 cells showing possible developed as a raw material for new therapeutics to ease the symptoms related with inflammatory.

수부 무지의 압궤 손상과 개방성 분쇄골절에서 역행성 도서형 요측 전완부 골피부 피판을 이용한 자료

이영근(Young-Keun Lee),우상현(Sang-Hyun Woo),김주용(Joo-Yong Kim),박지강(Ji-Kang Park),이항호(Hang-Ho Lee) 대한정형외과학회 2009 대한정형외과학회지 Vol.44 No.2

목적: 역행성 도서형 요측 전완부 골피부 피판을 이용한 무지 재건 치료 결과에 대해 보고하고자 한다. 대상 및 방법: 2001년부터 2005년까지 무지 재건을 위해 역행성 도서형 요측 전완부 골피부 피판술로 치료한 5예를 대상으로 하였다. 모두 남자였고, 수술 시 평균 연령은 45세였으며, 무지 결손의 형태, 피판의 크기, 채취된 요골의 길이, 골 유합까지의 시간, 무지의 관절 운동범위, 악력과 집기력, 합병증에 대해 알아보았다. 결과: 피판의 크기는 평균 58.4 ㎠였고 전위된 요골의 길이는 평균 44 ㎝였다. 모든 피판은 생존하였으며, 완전 골유합이 얻어 지기까지의 기간은 평균 2.8개월이었으며, 무지의 관절 운동범위는 평균 47도였다. 술 후 악력은 평균 94 lbs, 집기력은 14.6 lbs였으며, 피판부위 이동 이점 식별력은 평균 13 ㎜였다. 별다른 특이한 합병증은 없었다. 결론: 역행성 도서형 요측 전완부 골피부 피판은 혈관의 미세접합 수술없이 피부 및 뼈의 결손을 동반한 무지 손상에서 1단계로 재건 할 수 있는 유용한 피판으로 사료된다. Purpose: To report the outcome of a reversed osteocutaneous radial forearm flap for a thumb reconstruction. Materials and Methods: Five patients who underwent a reversed osteocutaneous radial forearm island flap for a thumb reconstruction between 2001 and 2005 were reviewed retrospectively. They were all males with a mean age at the time of surgery of 45 years. The types of thumb defects, size of the flap, length of the radius harvested, time to union, the range of motion of the thumb, grip and pinch strengths, and complications were analyzed. Results: The mean size of the transferred radial forearm flap was 58.4 ㎠. The mean length of the harvested radius was 4.4 ㎝. All flaps survived completely. The mean interval required to obtain bone union was 2.8 months. The mean ROM of the thumb was 47°. The postoperative mean grip strength was 94 Ibs and the mean pinch power was 14.6 Ibs. Mean moving two-point discrimination on the flap was 13 ㎜. There were no complications. All 5 patients were satisfied with the reconstructed new thumb. Conclusion: A reversed osteocutaneous radial forearm island flap is a useful procedure for reconstructing of a combined defect of the thumb.