昆蟲組織培養細胞(Antheraea eucalypti Ovarian Cells)에 있어서 日本腦炎바이러스 增殖

李淵台,蘇特 大韓微生物學會 1969 大韓微生物學會誌 Vol. No.

엘톨콜레라균에 대한 고찰

이연태 중앙대부설한국의과학연구소 1971 한국의과학 Vol.3 No.8

PS ( Y - 15') 세포상에서 아보·바이러스의 플라크 형성능

이연태,황기선,박길수 대한바이러스학회 1971 Journal of Bacteriology and Virology Vol.1 No.1

The plaque techniques of Dulbecco (1952) was first applied to the titration of arboviruses in culture of chicken fibroblast cells, also Poterfield (1959) described modificatory the plaque technique for titrating the infectivity of animal viruses. Most of arboviruses multiply in mammalian cells giving a variable amount of cytopathic degeneration. Some will produce plaque quite readily a monolayer of these cells under agar and agarose but other fail to do so. At a time when efforts were being made in this laboratory to improve the sensitivity of plaque reduction in mammalian cell by arboviruses, report were published (Miles, 1963 and Suitor, 1965) coneerning plaque production with arbovirus. They showed that several arboviruses produce minute plaques on monolayer under agar and agarose because it was inhibited by sulfate polysaccharide. They further demonstrated that this sulfated polysaccharide, which is a break down product regularly present in agar or agarose, can be adsorbed to and inactivated by diethylaminoethyl (DEAE)-dextran or protamine sulfate. When these substances were added to the agar or agarose in an appropriate dose the susceptible some arbovirus produced plaques quite as large and clear cut as those of the normal strain. This paper describes experimental modification of the plaque technique and reports obtained employing different overlay systems. The cells were used Inoues a clonal line from porcine kidney stable PS (Y-15') throughout this study. This cell cultured in Inoue's or Westaway's medium in 2 oz prescription bottle until monolayer formed. Virus strains, all of the 2 group A(Chikungunya, Western equine encephalitis) and 4 group B(Dengue II and IV, West nile, Yellow fever) arboviruses tested to produce plaques. Protamine sulfate and DEAE- dextran prepared as a various in distilled water. This stock solution were sterilized by autoelave(dextran) or filtration. They were added to the melted noble agar or agarose in appropriate amounts immediately before pouring. In those cases chikungunya virus, the plaque size are nearly same in diameter with each of six overlay systems but number of plaques are slightly difference. WEE virus that plaque size shows a range from small to large. The total number of plaques show a less than two fold difference from bottle to bottle. Dengue II virus with six overlay systems are seen. The plaque size shows a range from small (0.3 - 0.4) to large (l.5 - 2.0) The total number of plaque, both large and small, show a less than two fold difference from bottle to bottle. This virus shows variation in plaque size between agar and agarose overlay. In Dengue IV virus, plaque size shows a range from small to large and number of plaques were less than three fold difference in overlay systems. In West nile and Yellow fever virus, plaque size show small and large also total plaque number were variable under six overlay systems. West nile virus was effected with DEAE dextran in agarose overlay. The addition of these chemical reagents in optimal concentrations do not inhibit plaque sizes and number, those plaques by some viruses bear polymorphic appearances.

人體에서 分離한 葡萄糖 非醱酵菌의 抗菌劑 耐性에 關한 硏究

李淵台,崔承求,朴哲熙,曺圭鳳 단국대학교 대학원 1991 學術論叢 Vol.15 No.-

This experiment was conducted to classify for the 82 strains of glucose nonfermentative bacteria obtained from K hospital, in 1988, on the biological differentials and to test the resistance reaction on the drugs for those strains. The results were as follows : 1. Eighty two strains were classified into 65 strains of 79.3% of Ps. aeruginosa, 10 strains of 12.2% of Ps. cepacia, and 7 strains of 8.5% of Ac. calcoaceticus as results of 0 F sugar test, oxidase test, and DNase test. 2. It showed that Ps. aeruginosa was coincided with AP, CM, GM, KM, CF, and TC from the disk and MIC methods in the test of resistance reaction to antimicrobial agent, and especially, that AK and TOB KM and GM were observed high resistance reactive from MIC method. Resistnace to antibiotics for Ps. cepacia showed the identical reaction to AP, CB, CM, AK, SPT, CF, TC, TOB, KM and GM were observed high resistance reative from MIC method. Ac. calcoaceticus was identified the same resistance reaction to antibiotics to CB, KM, GM, AK, CF, and TOB, AP, SPT and TC showed high resistance reaction from the disk method. 3. CF and AP were observed the highest resistance reactions among 10 kinds of drugs used in this experiment and following was CM, KM and TC in order. 4. Ps. aeruginosa, Ps. cepacia, and Ac. calcoaeticus showed all multiple resistance reactions in the test of resistance to drugs, and Ps. aeruginosa was identified high resistance reaction to 6 drugs in 24.6%, Ps. cepacia was 9 drugs in 60%, and Ac. calcoaceticus showed resistance reaction to various kinds of drugs. 5. It showed important question in result of these studies that resistance percentage of NFB decreased by optimal antimicrobial selection and effective treatment established from serious antimicrobial treatment.

Salmonella 菌屬의 抗菌劑 耐性 및 傳達性 R-plasmid

李淵台,朴哲熙,曺圭鳳,洪承姬,裵建佑 檀國大學校 出版部 1991 論文集 Vol.25 No.2

이질균의 진단용 단클론 항체 개발에 관한 연구

이연태,조규봉,이운영 단국대학교 신소재기술연구소 1991 신소재 Vol.1 No.-

이질균의 항원구조는 매우 복잡하기 때문에 Shigella 균종 중에서 Shigella flexneri를 선택하여 이질균의 항원 특성을 규명하고자 본 실험을 시도하였다. 그래서 본 교실에서 분리 계대해 온 S. flexneri의 균체 항원을 2회에 걸쳐 마우스의 복강내로 주사하였다. 면역된 마우스의 비장에서 임파구를 분리하여 polyethylene glycol을 이용하여 골수종 세포인 P3X63-Ag8.653과 융합하였다. HAT 배지에서 융합 세포를 선택하였다. 무한대 희석법에 의하여 융합 세포의 클로닝을 실시하였으며 항체의 생산은 간접 효소면역흡착법을 이용하여 확인하고 다음과 같은 결과를 얻었다. 1. 세포 골수종 P3X63-Ag8.653의 평균 분열 주기는 대수 증식기에서 12시간이었다. 2. 융합이 실시된 20개의 plate 중에서 융합 세포가 자란 것은 15 well이었다. 3. 융합 세포가 형성된 15 well에서 항체 형성이 검증된 well은 3개였다. 4. 효소면역흡착법을 통해 알아본 각 well에서 생성된 면역글로불린의 class는 2 well에서 IgG, 1 well에서 IgM이었다. 5. 클로닝 후 IgM의 생성이 확인된 세포 배양 상청액을 이용하여 다른 장내세균의 균체 항원과 반응을 시켰으나 반응이 나타나지 않았다. The antigenic structure of Shigella is very complex. So, production of monoclonal antibodies against Shigella flexneri which had been the most prevalent Shigella spp. in Korea was tried to investigate the characteristics of shigella antigens. Heat-killed cellular antigens of S. flexneri which had been cultivated in our laboratory were injected intraperitoneally into BALB/c mice twice at intervals. Spleen lymphocytes of immunized mice were fused with the myeloma P3X63-Ag8.653 cells using polyethylene glocol. Only hybridized cells grew in HAT medium. Supernatants from cultures were assayed for S. flexneri-specific antibodies by the indirect enzyme-linked immunosorbent assay. Hybridomas were cloned by limiting dilution. 1. The mean doubling time of P3X63-A8.653 myeloma cells was 12 hours. 2. Only 15 wells out of 20 plates cell fusions performed showed the growth of hybridized cells in the selecive medium. 3. S. flexneri-specific antibodies were generated from 3 wells of those 15 wells, checked by indirect ELISA. 4. Supernatants from 3 wells had different classes of immunoglobulins : IgG from 2 wells and IgM from 1 well. 5. After cloning, supernatant from SFB 11 checked as Ig M with the strongest visuality was not reacted with cellular antigens of other strains of Enterobacteriaceae.

Vero E6 細胞에서 Semliki Forest Virus의 증식성에 관한 연구

이연태,강필원 대한바이러스학회 1988 Journal of Bacteriology and Virology Vol.18 No.1

In this study, Vero E6 cell, derived frorn African green monkey kidney, was selected as infectious host of Semliki forest virus and it was certified by cytopathic effect. Monolayer cells were infected with each serial diluted virus and maximum virus growth rate was obtained at 16 hours by freezing-solving methods. Ultra thin section samples were prepared to trace the infection route and the growth of virus. The majority of virus was trapped into coated pit region, internalized by endocytosis in coated vesicles and sequestered into intracellular vacuoles and lysosomes. Direct penetration of virus through the plasma membrane was never observed. After formation of coated vesicles, captured virus psrticles in lysosome were immediately released to cytoplasm and new virus particles were replicated there. Many virus particles were found in host cell organells, especially in vacuoles. Newly formed nucleocapsides acquired spike protein depending on host cell membrane by budding process, consequently new virus synthesis were completed. However, no penetration through nuclear membrane was observed. In the chromosome assay of viral infected cells, no significant chromosome aberration was found.

冷血動物(개구리)에서 日本腦炎 바이러스의 抗體보유에 관한 硏究

이연태,기영진,김광현,최성학,홍장선 단국대학교 신소재기술연구소 1993 신소재 Vol.3 No.-

일본뇌염 바이러스는 극동지방에서 여름철에 Culex tritaeniorhynchus 홍모기를 매개로 사람과 가축에 감염되여 질병을 유발하고 치명적인 결과를 초래하는 무서운 바이러스 질환의 원인체이다. 그동안 끊임없는 연구결과로 1970년 후반부터 일본뇌염 환자발생이 현저하게 감소하였다. 그러나 자연계에서 일본뇌염 바이러스의 생태학적 규명은 아직도 연구되어야 할 과제들이 산적되어 있다. 따라서 이 바이러스의 보유동물에 대한 자연계의 생활환경에 관한 연구가 필요하여 개구리의 항체보유여부를 규명 하였다. 6개도의 야외에서 채집한 개구리 681마리 혈청에 대하여 HI 방법에 의한 항체 분석을 시행하여 다음과 같은 결과를 얻었다. 1. 개구리 총 681마리의 일본뇌염바이러스에 대한 HI항체 보유율은 9.83%였다(p<0.01). 2. 일본뇌염바이러스에 대한 성별 HI 항체 보유율은 수컷이 2.63%(531마리 중 14마리). 암컷이 35.33%(150마리 중 53마리)로 암컷이 약 13배 높은 항체보유율을 나타냈다(p<0.01). 3. 종류별 HI항체 보유율은 북방산 개구리가 100%(1마리 중 1마리), 산개구리 66.66%(3마리 중 2마리), 금개구리 50.00%(36마리 중 18마리), 옴개구리 33.33%(6마리 중 2마리), 참개구리 6.92%(635마리 중 44마리)로 나타났다(p<0.01). 4. 월별 HI 항체 보유율은 11월이 66.66%(9마리 중 6마리)로 가장 높았으며 10월이 37.50%(32마리 중 6마리), 9월이 26.05%*142마리 중 37마리), 8월이 22.85%(35마리 중 8마리), 9월이 26.05%(142마리 중 37마리), 8월이 22.85%(35마리 중 8마리), 6월이 1.65%(241마리 중 4마리), 5월은 0%(222마리 중 0마리)이였다(p<0.01). 5. 지역별 일본뇌염바이러스에 대한 HI 항체보유율은 경기 16.56%(157마리 중 26마리, 충남 15.69%(172마리 중 27마리), 전북 7.14%(168마리 중 12마리)였고, 충북(71마리)과 강원(78마리)은 0%였다(p<0.010. Japanese Encephalitis virus(JEV) infects human and domestic animals via mosquitoes, Culex tritaeniorhynchus, and cause diseases that results in serious consequencies in Far East area during summer season. Thanks to the continuous research on this virus, the frequency of occurrence has been greatly diminished since the second half of 1970. But, there are still much work needs to be done on the virus, especially on their ecology in nature. We studied the antibodies in frogs based on the perspective that the research must be done on the carrier animals of the virus. We tested sera by HI method from 681 frogs captured randomyl in the six provinces. The results are summarized as following 1. Antibody positive rate to HI was 9.83%(67 out of 681). 2. Among these positive frogs, positive rate for male was 2.63%(14 out of 531), and positive rate for famale was 35.33%(53 out of 150). the positive rate for female is higher than that of male by about 13 times. 3. Different positive rates were shown among different species; 100%(1 out of 1) in Rana temporaria ornativentris, 66.66%(2 out of 3) in Rana temporaria orativentris, 50%(18 out of 36) in Rana plancyi chosenica, 33.33%(2 out of 6) in Rana rugosa and 6.92%(44 out of 635) in Rana niglomaculata. 4. When the monthly positive rates were compared, November was the highest as 66.66%(6 out of 9), 37.5%(6 out of 32) in October 26.05%(37 out of 142) in September, 22.85%(8 out of 35) in August, 65%(4 out of 241) in June and 0% in May 5. Areal distribution of positive rate to HI was 16.56%(26 out 157) in Kyung Kee, 15.69%(27 out of 172) in Chung Nam, 7.14%(12 out of 168) in Chun Buk, and 0% both in Chung Buk and Gang Won.

국내에 서식하는 박쥐에서 신증후 출혈열 원인바이러스에 대한항체분포에 관한 연구

이연태,박철희,조규봉,박은병 檀國大學校 基礎科學硏究所 1995 論文集 Vol.6 No.-

에이즈(AIDS)의 정체

이연태 단국대학 1987 檀苑 Vol.16 No.-