Flowcytometry를 이용하여 배양세포주에 있어서 인간단주항체 HMST-1이 인식하는 Type Ⅰ Sugar-Chain의 발현에 관한 연구

이순곤(SG Lee),남계현(KH Nam),조태호(TH Cho) 대한산부인과학회 1991 Obstetrics & Gynecology Science Vol.34 No.2

Changes in the expression of blood group antigens A, B and H(the antigen that defines blood group type) are known to occur in cancer tissues. The most common change is a loss or diminution of antigens A and B. This phenomenon is probably due to the incomplete biosynthesis of these antigens, which is associated with accumulation of precursor antigens. An increased rate of expression of Lewis type antigens, particularly Lewisb antigen in endometrial carcinoma, was observed in comparison to that in normal endometrium. In this study, flowcytometric analysis of the expression of type Ⅰ sugar-chain in cultured cell lines (uterine endometrial adenocarcinoma cell line. SNG-Ⅱ, uterine cervical epidermoid carcinoma cell line. SKG-Ⅲa) was carried out by using monoclonal antibody. 1. The type Ⅰ sugar-chain appeared frequently in SNG-Ⅱ cell membranes and rarely appeared in SKG-Ⅲa cell membranes. 2. But after sialidase treatment, amplified expression of type Ⅰ sugar-chain was observed especially in SKG-Ⅲa cells.

임신중 중증 자간전증 및 자간증에 대한 임상적 고찰

이기동(GD Lee),조충래(CR Cho),김수임(SI Kim),이순곤(SG Lee),이임순(IS Lee) 대한산부인과학회 1987 Obstetrics & Gynecology Science Vol.30 No.10

1980년 1월부터 1985년 12월까지 순천향 서울병원에서 분만한 총 9,105예 중 중증 자간전증 및 자간증 환자 170예와 순천향 구미병원에서 분만한 총 7,389예 중 중증 자간전증 및 자간증 환자 34예 및 1983년 8월부터 1985년 12월까지 천안 병원에서 분만한 총 2,481예 중 중증 자간전증 및 자간증 환자 91예를 대상으로 정상 대조군과 비교후 통계학적으로 서로 비교 분석하여 다음과 같은 결론을 얻었다. 1. 임신중 중증 자간전증 및 자간증의 발생 빈도는 서울 1.9%, 구미 0.5%였고, 천안 3.7%를 나타내어 이중 순천향 천안 병원의 발생 빈도가 가장 높은 빈도를 보였다. 2. 경증 자간전증은 구미 병원에서 88.5%로 가장 높았고, 중증 자간전증은 천안 병원에서 47.5%로 가장 높았고, 자간증은 천안 병원에서 9.5%로 가장 높은 치를 보였다. 3. 연령별 발생 빈도는 서울, 천안 병원에서는 40셰 이상에서 가장 높았으나, 구미 병원에서는 20세 미만에서 가장 높은 빈도를 보였다. 4. 분만횟수별 빈도를 보면 서울, 천안, 구미병원에서 모두 초산부에 가장 높은 빈도를 보였다. 5. 계절별 발생 빈도는 서울, 천안 병원에서는 각각 4, 3월게 가장 높았으나, 구미 병원에서는 1월에 가장 높았다. 6. 쌍태 임신의 발생 빈도는 단일 임신에 비해 서울, 천안, 구미 병원에서는 각각 7, 15, 7배로서 이중 천안 병원에서 가장 높은 빈도를 보였다. 7. 태아 성별에 의한 발생 빈도의 차이는 없었다. 8. 동반된 질환은 서울, 천안, 구미 병원에서 빈혈, 조기파수가 가장 많았고, 쌍태임신, 태반 조기박리등이 동반되었다. 9. 분만방법은 자연질식분만이 가장 높았으나 다른 보고자에 비해 낮으며 제왕절개술이 약간 높았으며 이중 천안 병원에서 39.6%로 가장 높았으나, 통계적 의의는 없었다. 10. 임신 기간은 서울, 구미 병원에서는 37∼40주에서 각각 61.2%, 55.9%로 가장 높았고, 천안 병원에서는 25주 미만에서 38.5%로 가장 높았다. 11. 신생아 체중은 단일 임신군보다 비교적 적었으며, 2,500gm미만이 서울 20%, 구미 32.4%였고, 천안 병원에서는 60.4%로 가장 높았다. 12. 주산기 합병증으로는 저체중 출생아, 사산아, 신생아 사망등이 있었으며, 천안 병원에서 가장 높은 빈도를 보였다. It is well known that pregnancy induced hypertension is one of the important problems in Obstetrics, which has one of the leading causes of maternal and perinatal mortality and morbidity. Despite extensive investigation along many different lines, the pathogenesis has remained obscure and the treatment was done only for symptoms and signs. So complete prenatal care can only prevent the development of pregnancy induced hypertension and complications. A clinical study was undertaken on 170 cases of severe form of preeclampsia and eclampsia among the 9105 pregnants who were admitted to Seoul and 34 cases among the 7389 pregnants to Kumi Soonchunhyang hospital for delivery from Jan. 1980 to Dec. 1985 and 91 cases among the 2481 pregnants admitted to Chunan Soonchunhyang hospital from Aug. 1983 to Dec. 1985. The results were as follows: 1. The total incidence of severe form of preeclampsia and eclampsia in pregnancies was 1.6%(295) of 18,975 deliveries. 2. The incidence of Seoul, Kumi and Chunan hospital was 1.9%, 0.5% and 3.7% rrespectively. The incidence of Chunan hospital was highest. 3. The rate of mild form of preeclampsia was highest at Kumi hospital(88.5%). The rate of severe form of preeclampsia was highest at Chunan hospital(47.5%). The rate of eclampsia was highest at Chunan hospital(9.5%). 4. At Seoul and Chunan hospital, the frequency of disease was higher in the age group above 40 years old but at Kumi hospital, higher under 20 years old. 5. According to parity, the higher frequency was noted in primipara at three hospitals. 6. Among 12 months, at Seoul and Chunan hospitals, the disease was more frequently seen in April and March but at Kumi hospital, higher in January. 7. The incidence of the disease in twin pregnancy was 7.0%, 7.0% and 15.0% at Seoul, Kumi and Chunan hospital respectively and then at Chunan hospital was highest.

염색체균형 전좌 보인자에 있어서 산전 세포유전학적 진단에 관한 연구

이충일(CI Lee),이순곤(SG Lee),김용진(YJ Kim),오신정(SJ Oh),김현승(HS Kim),최경준(KJ Choi),이권해(KH Lee) 대한산부인과학회 1997 Obstetrics & Gynecology Science Vol.40 No.9

Retrospective analysis of chromosomal abnormalities was performed on 75 couples who had recurrent spontaneous abortion. Karyotyping of their peripheral blood lymphocytes was made on 75 patients and 75 their husbands. The overall incidence of chromosomal aberrations was 22.7 %(17 in 75 couple). Number of chromosomal abnormalities in wives was twelve. Another chromosomal abnormalities were found in five husbands. All of them were balanced form and their phenotypes were normal. The incidence of translocations among total chromosomal abnormalities was 64.7 % (11/17). The incidence of reciprocal translocation(72.7 %) was higher than that of Robertsonian translocation (27.3 %). There were six other abnormalities including one case of inversion and five cases of polymorphisms. amniocentesis or chorionic villi sampling was performed during subsequent pregnancy to couples who had balanced chromosomal abnormalities. The karyotypes were classified as balanced translocation in seven cases, inversion in one case and normal karyotype in one case. This study suggests that prenatal diagnosis should be emphasized in couples in which one person is the carrier of a balanced chromosomal abnormality.

정상 임신부에서 혈청내 Interleukin-2와 Tumor Necrosis Factor-α 값의 변화

김동희(DH Kim),이순곤(SG Lee),최경훈(GH Choi),이슬기(SK Lee),양회경(HG Yang),이해혁(HH Lee),이임순(IS Lee) 대한산부인과학회 1999 Obstetrics & Gynecology Science Vol.42 No.7

Objective: Because the fetus exists immunologically as a semiallograft, pregnancy loss may be a manifestation of host versus graft rejection in some cases. So we undertook a preliminary study to determine IL-2 and TNF-α levels in serum during normal pregnancy. Methods: Samples were taken for serum TNF-α and IL-2 enzyme linked immunosolvent assays [ELISA: R&D systems, Minneapolis, MN] from 50 pregnant women without any complications, 30 healthy non pregnant women and 5 women with at least two previous abortions between 6-14 weeks. 5 women had symptoms of an threatened abortions as evidenced by a vaginal bleeding and ultimately had a third abortion. Statistical analysis was used student t test. Results: Serum IL-2 levels were determined respectively 11.0 22.5pg/mL and 66.2 47.5pg/mL in control and normal pregnant women. The peak IL-2 level was reached as early as the first month and was stable during pregnancy. Serum TNF-α levels were determined respectively 11.6 28.1pg/mL and 11.4 21.8pg/mL in control and normal pregnant women. Serum TNF-α levels in women with unexplained recurrent abortion were 29-34pg/mL. Conclusions: In normal pregnant women we found an increase in serum levels of IL-2 compared to the non-pregnant women. As regards serum levels of TNF-α, no differences could be observed between normal pregnant and non-pregnant women. In women with URA an increase in serum levels of TNF-α were found compared to the normal pregnant women.

Flow cytometry를 이용하여 자궁내막암에 대한 단주항체 MSN-1의 반응성에 관한 연구

김의홍(EH Kim),이순곤(SG Lee),조태호(TH Cho) 대한산부인과학회 1998 Obstetrics & Gynecology Science Vol.41 No.6

In the cytology, we sometimes have difficulties to make definitive diagnosis of endometrial cancers. In order to devise a new supplementary method, we used anti-endometrial adenocarcinoma monoclonal antibody MSN-1. In immunohistochemistry, MSN-1 reacted with more than 80% of endometrial adenocarcinomas. So, we analyzed the reactivities of MSN-1 with normal endometrium and endometrial adenocarcinoma by flowcytometry. First, in order to find out optimal conditions of sample treatment, we carried out some fundamental experiments using cultured SNG-II cell line which was the immunogen of MSN-1. They were clarified that mouse IgM had to be used as a control in every sample in order to subtract nonspecific reaction, and that the cell damage might be reduced by using collagenase dissolved in tissue culture medium. In clinical samples, 0.1% collagenase was used to disaggregate tissues, and cell suspensions were stained by indirect immunofluorescence technique. After filtered through 50 ㎛ nylon mesh, cells were analyzed by the flowcytometer FACscan (Becton Dickinson). All of 6 cases of endometrial adenocarcinomas showed positive rate of more than 30%, while 8 of 9 normal endometrium cases were under 30%, indicating that the application of MSN-1 to flowcytometry might be useful to differentiate endometrial cancer cells from normal ones.

인슐린과 표피성 성장인자가 자궁내막암세포주의 증식에 미치는 영향에 관한 연구

김주영(JY Kim),이순곤(SG Lee),조태호(TH Cho) 대한산부인과학회 1998 Obstetrics & Gynecology Science Vol.41 No.6

Endometrial adenocarcinoma is the common gynecologic malignancy in the U.S. Evidence is accumulating that links peptide growth factors with malignant proliferation. Epidermal growth factor (EGF) and insulin-like growth factor I (IGF-I) are known mitogen for endometrial adenocarcinoma in vitro. However, the biological activity of other growth factors in this malignancy is unclear. This study was undertaken to determine the influence of growth factors on the growth of the human endometrial adenocarcinoma cell line SCHE-1. Incubation with EGF and insulin stimulated the growth of SCHE-1 in time-dependent fashion. The number of SCHE-1 cells was increased 3.5-fold by EGF (100 ng/ml) and 2.2-fold by insulin (1000 ng/ml) when compared to untreated controls (p<0.05). These findings suggest that EGF and insulin may play a regulatory role in the proliferation of endometrial adenocarcinoma cell line.

Tamoxifen에 의해 증식이 억제된 자궁내막암 세포주의 가역성에 관한 시험관내 연구

원성윤(SY Won),이순곤(SG Lee),이순미(SM Lee),서희승(HS Seo) 대한산부인과학회 1996 Obstetrics & Gynecology Science Vol.39 No.11

Tamoxifen, which is often used in breast cancer therapy, has also been used in the treatment of patients with advanced and recurrent endometrial carcinoma. The growth inhibitory effects of tamoxifen were tested on the long-established endometrial carcinoma cell lines(RL 95-2) and on SCHE-1, a new endometrial carcinoam cell lines established in our laboratory. The MCF-7 breast cancer cell line was used as a control. Ten days of feeding with 5μM tamoxifen produced a 96% reduction in cell number in MCF-7 and a 83% reduction in SCHE-1 cultures. No viable cells remained in RL 95-2 cultures after 10 days of feeding with a 5μM tamoxifen. Tamoxifen-induced growth inhibition was reersible. All cell lines resumed logarithmic growth when tamoxifen was removed from the culture medium. In RL 95-2 and SCHE-1 cultures, addition of 17 β-estradiol to the culture medium did not accelerate recovery. Reversal of tamoxifen-induced growth inhibition was not seen when tamoxifen and 17 β-estradiol were added simultaneously. In MCF-7 cultures, tamoxifen-induced growth inhibition has been shown to be partially reversed by simultaneous use of 17 β-estradiol and 17 β-estradiol has been shown to increase the rate of recovery from tamoxifen blockade. Our results show that a potent growth inhibitory effect of tamoxifen is not reversed by estrogen and may thus be mediated through a mechanism different from blockade of estrogen receptor.

조기파수시 감염 예측도로서 태아호흡운동의 가치

최갑규(KK Choi),정구성(KS Jung),이순곤(SG Lee),유훈(H Yoo) 대한산부인과학회 1989 Obstetrics & Gynecology Science Vol.32 No.1

1986년 2월1일부터 1987년 1월31일까지 조기파수산모 73명을 대상으로 초음파를 사용하여 태아호흡운동을 관찰하여 다음과 같은 결론은 얻었다. 1. 감염군에서 태아호흡운동의 부재는 20명중 18명으로 감수성은 90.0%이다. 2. 비감염군에서 태아호흡운동의 존재는 53명중 34명으로 특이성은 64.2%이다. 3. 산모 73명중 태아호흡운동 부재는 37명이고 감염군에서는 20명중 18명으로 양성기대치는 48.6%이다. 4. 산모 73명중 태아호흡운동 존재는 36명이고 비감염군에서는 53명중 34명으로 음성기대치 는 94.4%이다. 이상과 같이 태아호흡운동 감지는 조기파수의 산모를 임상적으로 처치하는데 있어 결과를 향상시키는 비침투적이며 간결하고 확실한 진단법으로 사료된다. The antepartum fetal surveillance of patients with premature rupture of the membrane has not been accuratly defined. In the present study we examined the 73 patients with premature rupture of the membrane from Feb. 1986 to Jan.1978 The sensitivity and specificity of fetal breathing in predicting infection in patients with premature rupture of the membrane were 90.0% and 64.2% respectively. Presence of fetal breathing is a good predictor of noninfection outcome.

CA125를 생산하는 자궁내막선암 세포주의 수립과 동정 및 Dibutyl Cyclic Adenosine Monophosphate 에 의한 CA125 생산의 변화에 대한 연구

남계현 ( KH Nam ),이권해 ( KH Lee ),조태호 ( TH Cho ),김순덕 ( SD Kim ),이순곤 ( SG Lee ) 대한산부인과학회 1994 Obstetrics & Gynecology Science Vol.37 No.4

A new human endometrial carcinoma cell line, designated SCHE-1, was established from the endometrial adenocarcinoma of a 68-year-old woman. This cell line has grown well for 16 months and has been subcultured more than 50 times. Monolayer cultured cells are polygonal in shape, showing a pavement-lide arrangement and a piling up tendency without contact inhibition. The chromosomal number shows aneuploidy and the modal chromosomal number is in the diploid range. 1*10^5 SCHE-1 cells produced CA 125 (184-682U) during 19 days in cluture media. CA 125 was demonstrated immunohistocytochemically in the original tumor, and SCHE-1 cells. The cells contain no estrogen or progesterone receptors. We studied the effects of dibutyl cyclic adenosine monophosphate on celluiar growth and production of Ca125 in vitro. It is possible that the serum in the in vitro culture medium contains CA 125 and influences the activity of dibutyl cyclic adenosine monophosphate. Dibutyl cyclic adenosine monophosphate (which is said to have an influence upon gene expression) suppressed their proliferation and increased CA 125 levels. A dose-response relationship exists between the concentration of dbc AMP and CA 125 levels in the conditioned media, while the normal cells could not produce even after being administered dibutyl cyclic adenosine monophosphate. The above fact suggests that CA 125 gene expression was induced by dibutyl cyclic adenosine monophosphate.

산부인과 환자에서 악성 난소종양 발견을 위한 타액 및 혈액 CA 125 검출비교

남계현(KH Nam),전호용(HY Jeon),차상헌(SH Cha),이순곤(SG Lee),이권해(KH Lee),조태호(TH Cho) 대한산부인과학회 1993 Obstetrics & Gynecology Science Vol.36 No.2

Since Bast discovered the monoclonal antibody CA 125 for ovarian cancer, use of this antibody to estimate serum CA 125 has been established as a good tumor marker for detecting ovarian malignancy & follow-up after primary surgery. CA 125 is a glycoprotein complex that might also be found in saliva. So, we assaied the CA 125 levels from saliva and serum of 28 normal women, 38 benign pelvic mass patients and 34 gynecologic malignant patients and determined the correlation between saliva and serum CA 125 levels. The following results were obtained. 1. The CA 125 level of saliva was significantly higher by normal saline dilution than by free serum dilution. 2. The CA 125 level of saliva in normal control women (mean ±ISD: 1542±1468 U/ml) was significantly lower than those of benign pelvic mass patients(2823±2314U/ml) and gynecologic malignant patients(3142±2845U/ml). 3. The diagnostic efficiency of saliva CA 125 for detecting ovarian malignant tumors was equal to that of serum CA 125. 4. No correlation between saliva CA 125 and serum CA 125 was observed. 5. There were many cervical cancer patients who had normal serum CA 125 levels but had high saliva CA 125 levels over normal range.