인간포배기 배아의 효과적인 유리화 동결법의 개발을 위한 연구

이상민,이주희,이상원,이승민,윤산현,임진호,박흠대,이성구,Lee, Sang-Min,Lee, Ju-Hee,Lee, Sang-Won,Lee, Seoung-Min,Yoon, San-Hyun,Lim, Jin-Ho,Park, Huem-Dai,Lee, Seong-Goo 대한생식의학회 2003 Clinical and Experimental Reproductive Medicine Vol.30 No.3

Objective: The purpose of this study was to evaluate the survival rate of vitrified blastocyst according to the freezing vessels, equilibration time in cryoprotectant and artificial dehydration method. Methods: Human blastocysts were vitrified after loading onto the plastic straw, open-pulled straw (OPS), electron microscopy grid (EM grid) for 1.5 min or 3 min. They also were directly plunged into LN2 within 30sec. For artificial shrinkage of blastocysts, 36 gauge fine needle was pushed at the cellular junction of the trophectoderm into the blstocoele cavity until it shrank without damage of inner cell mass. Results: The survival rate of vitrified blastocysts on plastic straw, OPS, EM grid as freezing vessels were 26.7, 13.0 and 60.5%, respectively. The survival rate of EM grid was significantly higher than that of plastic straw and OPS (p<0.05). For 1.5 min equilibrium, the survival rates of early blastocyst (EB), middle blastocyst (MB) and late blastocyst (LB) were 64.4, 81.0, and 20.0% respectively. For 3 min equilibrium, the survival rates of EB, MB, and LB were 69.9, 50.0 and 57.5% respectively. The survival rates of EB and MB were significantly higher than that of LB in 1.5 min equilibrium group (p<0.05), however, the significance was not observed in 3 min equilibrium groups. In cytoplasmic shrinkage before vitrification, the survival rates of EB, MB and LB were 92.9, 100 and 75.9% respectively. The survival rate of MB was significantly higher than that of LB (p<0.05). The survival rates of vitrified blastocysts by artificial dehydration and slow-frozen blastocysts were not significantly different as 88.9 and 66.7%, respectively. Conclusion: This study showed that the vitrification of human blastocysts using EM grid and artificial dehydration is an effective method. Therefore, these methods would be an useful techniques for blastocyst cryopreservation.

정적 O/D자료와 실시간 동적 교통정보의 생성과 관리

이상민,신희철,유재광,Lee, Sang-Min,Sin, Hui-Cheol,Yu, Jae-Gwang 대한교통학회 2005 교통 기술과 정책 Vol.2 No.1

교육에서의 소진에 관한 이론적 고찰

이상민,안성희,Lee, Sang Min,An, Sunghee 연세대학교 의과대학 2014 의학교육논단 Vol.16 No.2

Burnout research has mostly been performed in human service fields. However, it has recently been expanded to cover diverse settings and even non-occupational samples, such as students. In this study, we defined the construct of academic burnout, which is the burnout experienced by students. Next, we described the concept of academic burnout by introducing measurements for assessing academic burnout. On the basis of the demand-control model (DCM) and effort-reward imbalance model (ERIM), which are the most predominant theoretical burnout models, we described the causal factors and the pathway to experiencing academic burnout symptoms. The ERIM was a more influential model than the DCM when explaining the academic burnout of Korean students. Based on the results of previous longitudinal studies on academic burnout, we recognized emotional exhaustion and academic inefficacy as the initial symptoms of academic burnout. Finally, we discussed the prevention and intervention programs with specific components that should be included in those programs.

『동아시아의 아카이브 비교연구』 곽건홍 저, 도서출판 선인, 2016

이상민,Lee, Sang-Min 한국기록학회 2016 기록학연구 Vol.0 No.49

Brunnstrom Stage (II)

이상민,Lee, Sang-Min 대한물리치료사협회 1986 물리치료 Vol.42 No.-

대식세포주에서 인슐린이 $I{\kappa}B/NF-{\kappa}B$ 경로 활성화에 미치는 영향

이상민,장연실,이춘택,김영환,한성구,심영수,유철규,Lee, Sang-Min,Jang, Yeon-Sil,Lee, Choon-Taek,Kim, Young-Whan,Han, Sung-Koo,Shim, Young-Soo,Yoo, Chul-Gyu 대한결핵및호흡기학회 2010 Tuberculosis and Respiratory Diseases Vol.68 No.3

Background: Sepsis still has a high mortality rate despite adequate supportive care. Newer therapeutic modalities have been developed but they have generally ended in failure. Recently, insulin was reported to have an anti-inflammatory effect by inhibiting the $I{\kappa}B/NF-{\kappa}B$ pathway, and may have therapeutic potential in sepsis. However, the precise mechanism of the anti-inflammatory effect of insulin is unclear. This study examined the role of insulin in activating $I{\kappa}B/NF-{\kappa}B$ in macrophage. Methods: Raw 264.7 cells, a murine macrophage cell line, were used in this experiment. Western blotting using $I{\kappa}B$ Ab and phosphor-specific $I{\kappa}B$ Ab was performed to evaluate the degradation and phosphorylation of $I{\kappa}B$ cells. For the $I{\kappa}B$ Kinase (IKK) activity, an immune complex kinase assay was performed. The level of interleukin-6 (IL-6) was measured by ELISA to determine the level of proinflammatory cytokine. Results: $I{\kappa}B{\alpha}$ degradation began 30 min after lipopolysaccharide (LPS) treatment. However, an insulin pretreatment suppressed the $I{\kappa}B{\alpha}$ degradation caused by the LPS treatment. The phosphorylation of $I{\kappa}B{\alpha}$ and IKK activity was also inhibited by the insulin pretreatment. Finally, the insulin pretreatment showed a tendency to suppress the induction of IL-6 by LPS. Conclusion: Insulin might have an anti-inflammatory effect though partial inhibition of the $I{\kappa}B/NF{\kappa}B$ pathway in macrophage cell lines.

녹색건축 전문가 자격제도 개발 연구

이상민,이승민,Lee, Sang Min,Lee, Seung Min 한국교육녹색환경연구원 2014 교육·녹색환경연구 Vol.13 No.1

In an effort to resolve environmental pollution problems, developed countries enforce green building certification systems. The same is true in Korea. However compared professional accreditation system to certification systems did not systematically in Korea. Therefore, in accordance with development of green building related systems and technology the requirement for foster green building professionals is urgent. Accordingly this study is purposed to analyze and propose that green building professional accreditation system development and operation of the direction. Through data analysis domestic and international green building professional accreditation system for draw a development plan. And then conduct an expert Delphi survey reflected in the survey results to development plan.

정보통신기술(Information & Communication Technology)을활용한 모바일 기반 정신건강개입의 현황과 한계

이상민,김승준,임우영,백종우,Lee, Sang Min,Kim, Seung-Jun,Im, Woo-Young,Paik, Jong-Woo 한국정신신체의학회 2016 정신신체의학 Vol.24 No.1

다양한 정보통신기술(Information & Communication Technology, ICT)들이 정신건강 영역에서 성공적으로 활용되고 있다. 특히 모바일 애플리케이션은 효과적인 ICT 중 하나이며, 스마트폰이 널리 보급된 이후 여러 앱들이 개발되고 있다. 모바일 기반 정신건강 개입은 치료 접근성 향상, 실시간 모니터링을 통한 증상 및 일상 활동의 손쉬운 확인과 같은 여러 장점이 있다. 치료 경과에서의 더 나은 추적 관찰, 개별화된 피드백과 향상된 이동성으로 인하여 환자들의 순응도를 향상시킬 수도 있다. 하지만 정신건강과 관련된 모바일 기술에는 전기통신 오류, 데이터 안전성 문제, 개인 정보 유출 같은 기술적인 애로사항들이 제한점으로 존재한다. 따라서 기술의 개발과 제공 과정에서 이에 대한 충분한 고려가 필요하다. 무엇보다도 정신건강 전문가들이 근거에 기반을 둔 경험들을 결합하고, 양질의 임상적 수준을 보장하면서 개발과정에 적극적으로 참여해야 할 것이다. Currently, a variety of Information and Communication Technology(ICT) is being broadly utilized for mental health. Especially, mobile application is one of the effective ICT, and several applications have been developed after the spread of smartphones. The mobile-based mental health has several strengths, such as better treatment accessibility and easier check-ups of symptoms or daily activities by real-time monitoring. Better follow-ups of treatment course, more customized feedback and better transportability enable patients to be more adherent. However, there are some limitations of mobile technology about the mental health, such as technical troubles of electric errors, data safety problems and personal information extrusion. Therefore, full considerations should be given during the development and provision of the technology. Most of all, mental health specialists should actively participate in the development process by incorporation of evidence-based experiences and assurance of good clinical qualities.

건강식생활 유형 및 건강증진 동기가 힐링체험 욕구에 미치는 영향

이상민,윤지영,정희선,Lee, Sang-Min,Yoon, Jiyoung,Jeong, Hee Sun 한국식품조리과학회 2015 한국식품조리과학회지 Vol.31 No.4

The purpose of this study was to examine the effects of a lifestyle consisting of a health oriented diet and personal motivation toward health improvement on an individual's desire to participate in condolence or "healing" -workshops and programs. In order to examine the influence of the "health diet" and motivation, the "health diet" lifestyle was classified into nutrition pursuit factors, psychological factors, food safety factors, and health conscious consumption factors. Personal motivation was categorized into perceived benefits, environmental factors and the individual's desire for happiness. Empirical analysis showed that nutrition pursuit factors had an impact on perceived benefits and the desire for happiness. Psychological aspect factors had a significant influence on perceived benefits, environmental factors and the desire for happiness. However, food safety factors and health conscious consumption factors did not have an effect on individual motivation to improve health. Regarding personal motivation toward health improvement on the desire for "healing", perceived benefits and environmental factors had significant influence. However, the individual's desire for happiness did not influence the demand for "healing". Regarding the effect of a "health diet" on the desire for "healing", only the psychological aspects of a "health diet" had any significant effects on the desire for "healing". The results from this study can help local organizations or service companies that provide "healing" programs with developing effective marketing strategies that could lead to greater customer satisfaction. Especially, the significant influence of psychological aspects of a "health diet" on the demand for "healing" is noteworthy in that this could play a key role in establishing a more effective marketing strategy to attract increasing numbers of consumers using health conscious diets.

폐 활성슬러지의 화학적 개질을 통한 비소의 생물흡착 특성

이상민,김동욱,Lee, Sang-Min,Kim, Dong-Wook 대한자원환경지질학회 2005 자원환경지질 Vol.38 No.5

비소를 제거하기 위한 경제적이고 친환경적인 생물흡착제 제조를 위해 폐 활성슬러지를 사용하여 생물흡착제로서의 적용성을 검토하였다. 생물흡착제로 사용된 폐 활성슬러지는 비소 흡착능 향상을 위해 각각 9시간과 24시간 메틸화(methylation) 되었고 흡착된 비소는 24시간 메틸화한 활성슬러지가 9시간보다 향상된 결과를 나타내었다. 24시간 메틸화한 활성슬러지의 비비소흡착량(specific As adsorption)은 0.06mmol As(V)/g biomass이고 용액의 pH가 비소에 미치는 영향은 생물흡착제로 사용된 활성슬러지가 메틸화된 후에도 지속되었다. 비소 제거에 대한 pH 영향은 5, 7, 9 세가지 경우에 대해서 조사되었고 이중 pH 5일때 비비소흡착량(specific As adsorption)이 pH 9인 경우보다 3배 큰 비흡착량을 나타내었다. Cheap and environmental sound biosorbent was made for the adsorption of arsenate using an waste activated sludge. The biosorbents were methylated in 9hours and 24 hours respectively for the better adsorption of arsenate. The amount adsorbed of arsenate(V) increased with increasing methylation time. The specific arsenate adsorption was 0.06mmol As(V)/g biomass when the biosorbent was methylated in 24 hours. The methylated biosorbents were also studied with pH 5, 7 and 9. The pH of the solution affect the amount of adsorption of arsenate of the biosorbent even though it was methylated. The specific arsenate adsorption of the biosorbent at pH 5 was best and it was three times greater than the amount of arsenate adsorbed at pH 9.