인간포배기 배아의 효과적인 유리화 동결법의 개발을 위한 연구

이상민,이주희,이상원,이승민,윤산현,임진호,박흠대,이성구,Lee, Sang-Min,Lee, Ju-Hee,Lee, Sang-Won,Lee, Seoung-Min,Yoon, San-Hyun,Lim, Jin-Ho,Park, Huem-Dai,Lee, Seong-Goo 대한생식의학회 2003 Clinical and Experimental Reproductive Medicine Vol.30 No.3

Objective: The purpose of this study was to evaluate the survival rate of vitrified blastocyst according to the freezing vessels, equilibration time in cryoprotectant and artificial dehydration method. Methods: Human blastocysts were vitrified after loading onto the plastic straw, open-pulled straw (OPS), electron microscopy grid (EM grid) for 1.5 min or 3 min. They also were directly plunged into LN2 within 30sec. For artificial shrinkage of blastocysts, 36 gauge fine needle was pushed at the cellular junction of the trophectoderm into the blstocoele cavity until it shrank without damage of inner cell mass. Results: The survival rate of vitrified blastocysts on plastic straw, OPS, EM grid as freezing vessels were 26.7, 13.0 and 60.5%, respectively. The survival rate of EM grid was significantly higher than that of plastic straw and OPS (p<0.05). For 1.5 min equilibrium, the survival rates of early blastocyst (EB), middle blastocyst (MB) and late blastocyst (LB) were 64.4, 81.0, and 20.0% respectively. For 3 min equilibrium, the survival rates of EB, MB, and LB were 69.9, 50.0 and 57.5% respectively. The survival rates of EB and MB were significantly higher than that of LB in 1.5 min equilibrium group (p<0.05), however, the significance was not observed in 3 min equilibrium groups. In cytoplasmic shrinkage before vitrification, the survival rates of EB, MB and LB were 92.9, 100 and 75.9% respectively. The survival rate of MB was significantly higher than that of LB (p<0.05). The survival rates of vitrified blastocysts by artificial dehydration and slow-frozen blastocysts were not significantly different as 88.9 and 66.7%, respectively. Conclusion: This study showed that the vitrification of human blastocysts using EM grid and artificial dehydration is an effective method. Therefore, these methods would be an useful techniques for blastocyst cryopreservation.

녹비작물을 이용한 윤작이 잡초 제어에 미치는 영향

이상민,이병모,이연,이용환,성좌경,윤홍배,최현석,Lee, Sang-Min,Lee, Byung-Mo,Lee, Youn,Lee, Yong-Hwan,Sung, Jwa-Kyung,Yun, Hong-Bae,Choi, Hyun-Sug 한국유기농업학회 2012 한국유기농업학회지 Vol.20 No.2

This study was conducted to evaluate the effects of crop rotation on the weed occurrence in green onion and red pepper cultivation. Rye and hairy vetch were annually seeded for winter season. The reduction of weed germination was pronounced by about 50% in green onion field, but was not significant in red pepper field through rye cropping. Among weed species, the germination of Abutilon theophrasti, Cyperus iria and Digitaria ciliaris was distinctly inhibited by exudate of rye root, but not by that of hairy vetch in vitro experiment. The return of rye biomass into onion field decreased amount of weed biomass but increased index of weed diversity.

헤어리베치(Vice villosa Roth) 품종별 국내 월동성, Biomass 및 무기태 질소 함량비교

이상민,이연,이용환,성좌경,윤홍배,최현석,Lee, Sang-Min,Lee, Youn,Lee, Yong-Hwan,Sung, Jwa-Kyung,Yun, Hong-Bae,Choi, Hyun-Sug 한국유기농업학회 2013 한국유기농업학회지 Vol.21 No.1

This study was conducted to evaluate overwintering of hairy vetch (Vicia villosa Roth), biomass, and inorganic N concentration in soil to verify the adaptability of eight cultivars of hairy vetch into country. Winter survival rate was higher for the hairy vetch cultivars, such as Hungvillosa or Ostsaat, than those of Minnie, Oregon common, and TTF1, which affected amount of biomass of each hairy vetch, with higher biomass observed in Hungvillosa or Ostsaat cultivar. There were no significant difference for the mineral nutrients of each hairy vetch cultivar. Inorganic N concentration in soil was increased at 10 days after mowing by application of Hungvillosa and Ostsaat cultivars that had highly produced N content from the raw materials.

인산형 연료전지 분리판용 천연흑연-불소수지계 복합재료의 흑연입도에 따른 전기비저항 변화

이상민,백운경,김태진,노재승,Lee, Sang-Min,Beak, Un-Gyeong,Kim, Tae-Jin,Roh, Jae-Seung 한국재료학회 2017 한국재료학회지 Vol.27 No.12

A composite material was prepared for the bipolar plates of phosphoric acid fuel cells(PAFC) by hot pressing a flake type natural graphite powder as a filler material and a fluorine resin as a binder. Average particle sizes of the powders were 610.3, 401.6, 99.5, and $37.7{\mu}m$. The density of the composite increased from 2.25 to $2.72g/cm^3$ as the graphite size increased from 37.7 to $610.3{\mu}m$. The anisotropy ratio of the composite increased from 1.8 to 490.9 as the graphite size increased. The flexural strength of the composite decreased from 15.60 to 8.94MPa as the graphite size increased. The porosity and the resistivity of the composite showed the same tendencies, and decreased as the graphite size increased. The lowest resistivity and porosity of the composite were $1.99{\times}10^{-3}{\Omega}cm$ and 2.02 %, respectively, when the graphite size was $401.6{\mu}m$. The flexural strength of the composite was 10.3MPa when the graphite size was $401.6{\mu}m$. The lowest resistance to electron mobility was well correlated with the composite with lowest porosity. It was possible the flaky large graphite particles survive after the hot pressing process.

개선된 RBI 기법을 이용한 석유화학설비의 위험도평가

이상민,송기훈,장윤석,최재붕,김영진,한상인,최송천,Lee Sang-Min,Song Ki-Hun,Chang Yoon-Suk,Choi Jae-Boong,Kim Young-Jin,Han Sang-In,Choi Song-Chun 대한기계학회 2005 大韓機械學會論文集A Vol.29 No.10

API 581 guideline provides a methodology for calculating the risks of equipments in refinery or petrochemical plant. However, especially in part of the consequence of failure, there is a major limitation of its application to the petrochemical plant directly since only a representative material is considered in calculating the risk while the equipment is composed of numerous materials. The objectives of this paper are to propose an enhanced risk-based inspection (RBI) technique to resolve shortcomings inclusive of the above issue and to assess the risks of typical petrochemical equipments. In this respect, a program incorporating material database was developed to fully incorporate the characteristics of different materials. The proposed RBI program consists of qualitative, semi-quantitative and quantitative risk evaluation modules in which toxic materials as well as representative materials were selected automatically for comparison to those in the current guideline. It has been applied to assess the risks of equipments in ethylene facilities of petrochemical plants. Thereby, more realistic evaluation results were obtained and applicability of the proposed RBI program was proven.

액와부 내시경 갑상선 절제술 후 나타날 수 있는 합병증에 대한 고찰

이상민,김성흔,정갑중,Sang Min Lee,Sung Heun Kim and Ghap Joong Jung 대한갑상선-내분비외과학회 2010 The Koreran journal of Endocrine Surgery Vol.10 No.3

Essentiality of Dietary Eicosapentaenoic Acid and Docosahexaenoic Acid in Korean Rockfish, Sebastes schlegeli

이상민,이종윤,허성범,LEE Sang-Min,LEE Jong Yun,HUR Sung Bum The Korean Society of Fisheries and Aquatic Scienc 1994 한국수산과학회지 Vol.27 No.6

Feeding trials were conducted to determine the essentiality of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), and to compare the efficiency between EPA and DHA for juvenile Korean rockfish. Fish averaging 2.1 g were fed experimental diets containing different levels of EPA or DHA, and different combinations of EPA and DHA in two separate experiments. Graded levels ($0.0{\sim}l.75\%$) of dietary EPA or DHA as ethyl esters were substituted for a part of the $8\%$ beef tallow in the basal diet. After 5 weeks of the experimental period, weight gain, chemical composition of whole body, protein and lipid retention efficiency, hepatosomatic index, and fatty acid composition of liver were measured. Daily growth rate and feed efficiency were the lowest in fish fed the diets without EPA arid DHA. These responses were effectively improved by increasing EPA or DHA in the diets up to $1.0\%$ levels, and then reached a plateau between 1.0 and $1.75\%$ levels of either EPA or DHA. Protein and lipid retention efficiency were also improved with the high levels of dietary EPA or DHA. DHA was superior to EPA at the same level of each in weight gain, feed efficiency, and protein and lipid retention efficiency. Hepatosomatic index tended to decrease with increase of the dietary EPA or DHA levels. Lipid contents of whole body were increased with levels of EPA or DHA in the diets. Dietary EPA and/or DHA levels affected directly the fatty acid composition of liver polar lipid. EPA or DHA in the liver polar lipid were increased with levels of dietary EPA or DHA, respectively, whereas those in nonpolar lipid were not affected by the dietary levels of EPA and/or DHA. These finding indicate that either of the EPA or DHA is essential for normal growth of Korean rockfish, and the essential fatty acid requirement is $1.0\%$ of EPA and/or DHA in the diet. DHA is superior to EPA as essential fatty acid, and the dietary EPA/DHA ratio of less than 1.0 may be adequate for normal growth of Korean rockfish fed a diet enough n-3HUFA (EPA and DHA).

Brunnstrom Stage (II)

이상민,Lee, Sang-Min 대한물리치료사협회 1986 물리치료 Vol.42 No.-

조피볼락 사료의 어분 대체 단백원으로서 대두박 평가

이상민,전임기,LEE Sang-Min,JEON Im-Gi 한국수산과학회 1996 한국수산과학회지 Vol.29 No.5

조피볼락 사료의 어분 대체 단백원으로서 대두박의 이용성을 검토하기 위해, 북양어분을 주단백원으로 한 대조사료, 0.25mm로 분쇄한 대두박을 $5\%$ 간격으로 $20\%$까지 첨가한 사료, 대두박 $20\%$ 첨가 사료에 아미노산 (met+lys) 보충 사료, 0.5mm로 분쇄한 대두박 $20\%$ 첨가 사료 및 대두박 $10\%$에 콘글루텐 밀과 육골분을 각각 $10\%$ 혼합 첨가한 사료를 제조하여, 22.9g 전후의 조피볼락을 9주간 사육 실험하여 성장 효과, 체성분 조성 및 외견상 소화율을 조사하였다. 0.25mm로 분쇄한 대두박을 $20\%$까지 단독으로 첨가하여도 최종 평균체중, 증체율, 사료효율, 일일사료 섭취율 및 단백질축적율이 대조구와 유의차없이 (P<0.05) 좋은 결과를 보였고, 대두박 $20\%$ 첨가구에 methionine 및 lysine를 각각 $0.5\%$씩 보충한 실험구에서도 성장효과는 개선되지 않았다 대두박의 분쇄 입자에 따른 효과를 검토하기 위해 0.5mm로 분쇄한 대두박을 $20\%$ 첨가한 실험구는 사료효율, 일일사료섭취율 및 단백질축적율에서 0.25mm로 분쇄한 대두박 $20\%$ 첨가구와는 유의차가 없었지만 (P>0.05), 최종 평균체중 및 증체율은 대조구와 대두박 $5\%$ 첨가구에 비해 낮은 값을 보였다 (P<0.05). 또한, 대두박 $10\%$+콘글루텐 밀 $10\%$+육골분 $10\%$ 첨가구도 최종 평균체중, 증체율, 사료효율, 일일사료섭취율 및 단백질축적율에서 대조구와 유의차가 없어 (P<0.05), 여러 가지 원료를 적절히 혼합 첨가하는 것이 대두박을 단독으로 첨가하는 것 보다 더 효율적으로 어분을 대체할 수 있을 것으로 판단된다.

조피볼락 육성용 사료의 어분 대체원으로서 식물성 및 동물성 단백질 혼합 첨가 효과

이상민,전임기,이종윤,박승렬,강용진,정관식,LEE Sang-Min,JEON Im-Gi,LEE Jong-Yun,PARK Sung-Real,KANG Yong-Jin,JEONG Kwan-Sik 한국수산과학회 1996 한국수산과학회지 Vol.29 No.5

A 15-week growth trial was conducted in flow-through aquarium system to develop practical feeds for growing Korean rockfish. Two replicate groups of the fish averaging 114 g were fed one of nine diets containing $45\~50\%$ crude protein from various practical ingredients such as fish meal, meat meal, feather meal, blood meal, soybean meal, corn gluten meal, and wheat flour with or without supplemental essential amino acids (EAA) or enzyme mixture. The dietary EAA were adjusted by considering EAA composition of each dietary protein source, A/E ratio (each essential amino $acid\times1000/total$ essential amino arid including Cys and Tyr) calculated using Ah composition of the Korean rockfish whole body and the EAA requirement of other fish. Results indicate that animal and plant protein sources could substitute for fish meal up to $50\%$ in the diets, and the supplementation of amino acids and enzyme mixture have no beneficial effects on fish performance. Fish growth, body composition, nutrient utilization, and cost of fish production are discussed in relation to nutritional values of the protein sources used in diets.