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봄철 수벌 유충 트랩에 의한 꿀벌(Apis mellifera L.) 봉군의 꿀벌응애(Varroa destructor) 방제 효과
이명렬(Myeong-Lyeol Lee),박영미(Young-Mi Park),이병일(Bing-Ri Li) 한국양봉학회 2004 韓國養蜂學會誌 Vol.19 No.2
As one of the alternative control methods of Varroa mites to synthetic pyrethroid chemicals, the drone brood trapping was evaluated. Normal worker comb frames were divided into three parts, then, in the center or both sides of which natural drone cells were constructed and drone larvae were reared as attracting traps of Varroa mites. In the first trial carried out in early June, the numbers of bee mite females entered to the 100 capped drone larvae were 5.7 ± 3.2 in center traps and 8.0 ± 5.6 in sides traps; however, there was only one mite found in 900 worker larvae. From the second trial in late June, the colonies where drone traps were installed in center part of frame attracted 17.0 ± 3.0 mites into drone larvae and 1.7 ± 1.5 mites into worker larvae in normal comb. Bee mites of 8.0 ± 4.0 and 1.0 ± 1.7 were found on drone and worker larvae in sides traps. Drone larvae were 8 to 10 times more attractive to Varroa mites than worker larvae. When the number of mites in worker cells was simply compared, drone trapping colonies showed 21% ~ 36% of the mite density in un-trapped colonies(4.7 ± 4.0 mites). The number of mites monitored from worker larvae in the 40th day after the second trial ranged 4.7 ~ 12.3 in experimental groups, which did not show any significant differences. If the colonies without brood area like swarming or mating colonies can be applied by these drone traps, more reliable effects against bee mites should be expected.
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백묵병 감염유충으로부터 병원균의 분리 및 형태학적 특성
남성희(Sung Hee Nam),이명렬(Myeong Lyeol Lee),이만영(Man Young Lee),김영수(Young Soo Kim),이병일(Ri Bing Li),장승종(Seung Jong Chang),윤병수(Byoung Su Yoon) 한국양봉학회 2004 韓國養蜂學會誌 Vol.19 No.1
Chalkbrood-infected larvae of honeybee were obtained from 8 apiaries in Korea. Total 548 samples varied in the rate of black and white mummies of honey bee. Among them, 306 black mummies were examined to identify species. The whole larvae were proved to be infected with Ascosphaera apis(Maasen ex Claussen) Olive & Spiltoir. In the early stage of chalkbrood diseases, the white/grey mass was formed on the surface of larvae. The larvae were soon shrunken to mummy, getting hard, and forming hard mummies. In the late stage of chalkbrood diseases, the mycelium grew densely and covered the larvae to the extent that it filled the whole cell. When the larvae were infected with one sexual type of A. apis, they became white mummies while the grey-black mummies were developea by the invasion of both sexual types. A. apis (Aaj623) was heterothallic and mostly grown 57.6 ㎜ in diameter in 7 days on potato dextrose agar(PDA). Spore cysts were globose, nearly smooth on outer surface and evenly verrucate on inner surface, 45~95 ㎛ in diameter(average 74 ㎛ diameter). Spore balls were globose, 9~17 ㎛ in diameter(average 12.9 ㎛ in diameter) and lacking a conspicuous granular coating. Ascospores were hyaline, ellipsoid and smooth, 1 cell, 2.3~3.0 × 1.0~1.5 ㎛(average 2.49 × 1.35 ㎛ in diameter).
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