현수교 앵커구체의 수화열 관리

이두재,이성범 한국콘크리트학회 2000 콘크리트학회지 Vol.12 No.5

Reactive Oxygen Species and Tumor Metastasis

이두재,강상원 한국분자세포생물학회 2013 Molecules and cells Vol.35 No.2

The migration and invasion of cancer cells are the first steps in metastasis. Through a series of cellular re-sponses, including cytoskeletal reorganization and degradation of the extracellular matrix, cancer cells are able to separate from the primary tumor and metastasize to distant locations in the body. In cancer cells, reactive oxygen species (ROS) play important roles in the migration and invasion of cells. Stimulation of cell surface receptors with growth factors and integrin assembly generates ROS, which relay signals from the cell surface to important signaling proteins. ROS then act within cells to promote migration and invasion. In this review, we collect recent evidence pointing towards the involvement of ROS in tumor metastasis and discuss the roles of ROS at different stages during the process of cancer cell migration, invasion and epithelial-mesenchymal transition.

Retraction Note: Reactive Oxygen Species and Tumor Metastasis

이두재,강상원 한국분자세포생물학회 2013 Molecules and cells Vol.35 No.4

The migration and invasion of cancer cells are the first steps in metastasis. Through a series of cellular responses, including cytoskeletal reorganization and degradation of the extracellular matrix, cancer cells are able to separate from the primary tumor and metastasize to distant locations in the body. In cancer cells, reactive oxygen species (ROS) play important roles in the migration and invasion of cells. Stimulation of cell surface receptors with growth factors and integrin assembly generates ROS, which relay signals from the cell surface to important signaling proteins. ROS then act within cells to promote migration and invasion. In this review, we collect recent evidence pointing towards the involvement of ROS in tumor metastasis and discuss the roles of ROS at different stages during the process of cancer cell migration, invasion and epithelial-mesenchymal transition.

초탄성 형상기억합금 능동제어 가새시스템을 이용한 중 심가새프레임 구조물의 지진거동 및 복원성능 평가

허종완,이두재,조양희 한국지진공학회 2012 한국지진공학회논문집 Vol.16 No.6

The researches related to active control systems utilizing superelastic shape memory alloys (SMA) have been recently conducted to reduce critical damage due to lateral deformation after severe earthquakes. Although Superelastic SMAs undergo considerable inelastic deformation, they can return to original conditions without heat treatment only after stress removal. We can expect the mitigation of residual deformation owing to inherent recentering characteristics when these smart materials are installed at the part where large deformation is likely to occur. Therefore, the primary purpose of this research is to develop concentrically braced frames (CBFs) with superelastic SMA bracing systems and to evaluate the seismic performance of such frame structures. In order to investigate the inter-story drift response of CBF structures, 3- and 6-story buildings were design according to current design specifications, and then nonlinear time-history analyses were performed on numerical 2D frame models. Based on the numerical analysis results, it can be comparatively verified that the CBFs with superelastic SMA bracing systems have more structural advantages in terms of energy dissipation and recentering behavior than those with conventional steel bracing systems.

3-nitropropionic Acid 투여 후 마우스 선조체에서 Apurinic/Apyrimidinic Endonuclease의 발현과 신경세포고사

조경주,이두재,이병인,김경환 대한신경과학회 2005 대한신경과학회지 Vol.23 No.4

Background: 3-Nitroporpionic acid (3-NP) is an irreversible inhibitor of succinate dehydrogenase in mitochondria and can induce apoptosis-like cell death in the striatum. It has been reported that oxidative stress plays a role in the 3-NP induced neuronal damage. 3-NP induced striatal damage is implicated in the pathogenesis of several neurological diseases, such as chronic neurodegenerative diseases and stroke. The DNA repair enzyme, apurinic/apyrimidinic endonuclease (APE), is a multifunctional protein in the DNA base excision repair (BER) pathway. To clarify the relationship between APE and neuronal cell death associated with the apoptosis in the striatum was induced by 3-NP in vivo. Methods: After intra-striatal injection of 3-NP, expression of the APE protein and mRNA were evaluated by Western blot, immunohistochemistry, RT-PCR and DNA fragmentation patterns. Oxidative DNA damage was investigated by detection of oxidized DNA, AP site and superoxide. Results: Expression levels of APE was rapidly reduced as early as 1hr after injection of 3-NP. DNA fragmentation was observed 24 hours after 3-NP treatment but not 4 hours. APE gene expression was increased to 1hr after 3-NP treatment. The number of AP sites were reduced and the reduction of APE proteins were blocked by a superoxide scavenger, MnTBAP-treatment. Conclusions: These results suggest that the reduction of APE is the preceding event of DNA fragmentation that causes apoptosis and a decrease of APE may be induced by ROS after 3-NP treatment.

편측 척추경외 접근에 의한 추체 성형술의 유용성과 술기

안성준,송무호,김부환,유성호,이두재 대한척추외과학회 2011 대한척추외과학회지 Vol.18 No.4

Study Design: A retrospective study. Objectives: To evaluate the effectiveness of unilateral extrapedicular approach in the treatment of osteoporotic compression fracture,as compared to transbipedicular approach. Summary of Literature Review: There has been no comparative study assessing this topic. Materials and Methods: 115 patients presenting with percutaneous vertebroplasty between Mar. 2002 and Feb. 2009, were divided into three groups: Group A (43 vertebrae; 29 patients) who were treated with bipedicular approach, Group B (66 vertebrae; 47 patients)treated with early cases of unilateral extrapedicular approach, and Group C (43 vertebrae; 39 patients) treated with late cases of unilateral extrapedicular approach. We analyzed radiological test results including the volume of injected cement and the distribution of intravertebral body, cement leakage, height restoration and kyphosis correction. Statistical analysis was done using SPSS. Clinical results were analyzed using VAS scores. Results: The mean follow-up period varied from one year at minimum to seven years and six months at maximum. The mean volume of injected cement was 3.39cc/5.39cc/3.79cc for groups A, B and C respectively. Cement leakage was at 13.4/34.8/12.8% in each group. Cement leakage was higher in group B, but most leakage took place in early cases that we tried to inject more and more cement in early inexperienced period. Bilaterally well distributed cement in the vertebral body was at 85.7/76.9% in groups B and C respectively. VAS scores improved from 8.4/8.3/8.5 preoperatively to 2.0/2.0/1.6 postoperatively. Conclusions: Percutaneous vertebroplasty treated with unilateral extrapedicular approach can lessen perioperative operating 연구 계획: 후향적 분석목적: 편측 척추경외 접근법에 의한 추체 성형술의 술기와 양측 척추경 성형술과 비교하여 유용성을 알아보고자 하였다. 선행문헌의 요약: 이에 관한 비교 논문은 없었다. 대상 및 방법: 2002년 3월부터 2009년 2월까지 척추 압박골절로 내원한 446명 중 보존적 치료에 실패한 115명의 환자 152예의 경피적 추체 성형술을대상으로 하였다. 2002년 3월부터 2004년 9월까지 양측 척추경 접근법에 의해 추체 성형술을 시행한 군을 A군(29명, 43추체), 2004년 9월부터 2006년6월까지 편측 척추경외 접근법을 사용하여 추체 성형술을 시행한 군을 B군(47명, 66추체), 2006년 7월부터 2009년 2월까지 편측 척추경외 접근법을사용하여 추체 성형술을 시행한 군을 C군(39명, 43추체)으로 나누어 시멘트의 삽입량, 방사선학적 평가로서 추체높이의 복원과 교정력, 시멘트의 추체내 분포, 누출 등을 평가하였고 임상적 평가로서 술 전과 술 후 VAS를 사용하였다. 결과: 평균 추시기간은 최소 12개월-7년 6개월이었다. 삽입된 시멘트의 양은 A/B/C 각군ㅇ서 평균 3.39cc/5.39cc/3.79cc였다. 평균 시멘트 누출은 A군에서 13.4%, B군에서 34.8% 였으나 B군에서 시멘트 누출은 주로 초기 증례였고, C군에서 시멘트 누출은 12.8%였다. B군에서 추체내 양측에 시멘트가 골고루 분포한 경우는 85.7%였고, C군에서 추체내 양측에 시멘트가 골고루 분포한 경우는 76.9%였다. 임상평가로서 VAS score는 술 전 각 군에서8.4/8.3/8.5에서 1.8/2.1/3.1, 술 후 평균 최종 추시상 2.0/2.0/1.6으로 양군에서 모두 양호한 결과를 보였다. 결론: 편측 척추경외 접근법을 이용하여 추체 성형술을 시행하는 경우, 편측 도관 삽입과정으로 인한 수술시간의 단축과 더불어 기술적으로 세심하게시술한다면 방사선학적으로 추체내 좌 우 양측에 골고루 충분한 양의 시멘트의 삽입, 추체높이의 복원과 교정력도 양측 척추경 접근법에 의한 경우와비교하여 차이가 없었으며 임상결과도 양호하였다.

흡수성 봉합사를 이용한 무지외반증 Akin 절골술

송무호,김부환,안성준,유성호,이두재,Song, Moo-Ho,Kim, Bu-Hwan,Ahn, Seong-Jun,Yoo, Seong-Ho,Lee, Doo-Jae 대한족부족관절학회 2011 대한족부족관절학회지 Vol.15 No.3

Purpose: The Akin osteotomy which is a closing wedge osteotomy of the proximal phalanx widely used for the correction of hallux valgus has several methods of fixation. we tried to report the effects of the fixation using an absorbable suture material during the Akin osteotomy for the hallux valgus. Materials and Methods: This study was based on 448 cases of 346 patients who were able for follow-up more than 12 months among the patients who had an Akin osteotomy together with the surgery of hallux valgus between March of 2006 and May of 2010. Absorbable suture material had been used in all cases. Radiologically displacement and union of osteotomy site were observed after the surgery, and clinically postoperative complication such as skin irritation, pain and satisfaction were investigated. Results: Radiologically all cases had showed complete union and no case had the loss of an correction due to loss of fixation. Also, any case had no skin irritation due to a knot. Three cases had a medial cortical breakage due to a strong knot, and the initial one case among them had additionally fixed the osteotomy site for four weeks using K-wire, and the remaining two cases had fixed a suture on an articular surface without any fixation of an additional wire. If a medial cortical bone was lost by carrying out an ostectomy due to proximal protrusion of proximal phalanx, three cases could show union through the fixation of suture on an articular surface. Conclusion: This study considers that the fixation of the osteotomy site using an absorbable suture material in an Akin osteotomy was effective method and the advantage of this procedure was unnecessity of the material removal and no skin irritation.

AISC 2005 코드를 활용한 콘크리트 충전 합성기둥의 해석과 평가

박지웅 ( Ji Woong Park ),이두재 ( Doo Jae Rhee ),장성수 ( Suong Su Chang ),허종완 ( Jong Wan Hu ) 한국복합신소재구조학회 2012 복합신소재학회논문집 Vol.3 No.3

Concrete filled steel tube (CFT) columns have been widely used in moment resisting frame structures both in seismic zones. This paper discusses the design of such members based on the advanced methods introduced in the 2005 AISC Specification and the 2005 Seismic Provisions. This study focuses particularly on design following both linear and nonlinear methods utilizing equivalent static and dynamic loads for low-rise moment frames. The paper begins with an examination of the significance of pseudo-elastic design interaction equations and the plastic ductility demand ratios due to combined axial compressive force and bending moment in CFT members. Based on advanced computational simulations for a series of five-story composite moment frames, this paper then investigates both building performance and new techniques to evaluate building damage during a strong earthquake. It is shown that 2D equivalent static analyses can provide good design approximations to the force distributions in moment frames subjected to large inelastic lateral loads. Dynamic analyses utilizing strong ground motions generally produce higher strength ratios than those from equivalent static analyses, but on more localized basis. In addition, ductility ratios obtained from the nonlinear dynamic analysis are sufficient to detect which CFT columns undergo significant deformations.

Silencing of peroxiredoxin II by promoter methylation is necessary for the survival and migration of gastric cancer cells

홍수현,민성춘,전유경,이두재,김승화,박주현,정재호,박윤정,김수열,이상혁,강상원 생화학분자생물학회 2018 Experimental and molecular medicine Vol.50 No.-

Peroxiredoxin (Prx), a family of ubiquitous thiol peroxidases, functions as a redox signaling regulator that controls cellular H2O2 in mammalian cells and has recently received attention for being overexpressed in various cancer types. In this study, we show that Prx type II (PrxII) is rather silenced in gastric cancer cells. PrxII expression is severely downregulated in 9 out of the 28 gastric cancer cell lines. Strikingly, PrxII expression is completely lost in three cell lines, MKN28, MKN74 and SNU484. Loss of PrxII expression is due to DNA methyltransferase 1-dependent methylation at the promoter region of the PrxII gene. Restoration of PrxII expression using a retroviral system markedly reduces the colony-forming ability and migratory activity of both MKN28 and SNU484 cells by inhibiting Src kinase. Mechanistically, PrxII peroxidase activity is essential for regulating gastric cancer cell migration. Bioinformatics analysis from The Cancer Genome Atlas stomach cancer data (STAD) revealed significantly low PrxII expression in gastric cancer patients and a negative correlation between PrxII expression and methylation levels. More importantly, low PrxII expression also strongly correlates with poor survival in cancer patients. Thus our study suggests that PrxII may be the first thiol peroxidase that simultaneously regulates both survival and metastasis in gastric cancer cells with high clinical relevance.

폐선부지 철도자갈 재활용방안 연구

공병근(Kong Byeong-Keun),권진수(Kueon Jin-Su),이두재(Lee Doo-Jae),김수정(Kim Soo-Jeong) 한국철도학회 2010 한국철도학회 학술발표대회논문집 Vol.2010 No.10