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축산업 분야에서의 박테리오신의 산업적 이용 및 향후 전망
이나경,이주연,곽형근,백현동,Lee, Na-Kyoung,Lee, Joo-Yeon,Kwak, Hyung-Geun,Paik, Hyun-Dong 한국축산식품학회 2008 한국축산식품학회지 Vol.28 No.1
More safe and natural food was recently needed by consumers. Antimicrobials including sodium azide, penicillin, and vancomycin were used for therapeutic agents against pathogens such as Listeria monocytogenes, Staphylococcus aureus, Escherichia coli O157:H7 in dairy and meat industry. These antimicrobials and preservatives were prohibited in stock farm and food because they were caused resistant strain and side effects. Bacteriocins are proteinaceous compounds that may present antimicrobial activity towards important food-borne pathogens and spoilage-related microflora. Therefore, bacteriocins were reported as an alternative of antimicrobials. Due to these properties, bacteriocin-producing strains or purified bacteriocins have a great potential of use in biologically based food preservation systems. Despite the growing number of articles regarding on the isolation of bacteriocinogenic strains, genetic determinants for production, purification and biochemical characterization of these inhibitory substances, there are only limited reports of successful application of bacteriocins to dairy and meats. This review describes bacteriocins related to dairy and meat products for the further use.
이나경,박여랑,김현욱,박용호,임성렬,김종만,김재명,남향미,정석찬,백현동,Lee, Na-Kyoung,Park, Yeo-Lang,Kim, Hyoun-Wook,Park, Yong-Ho,Rhim, Seong-Lyul,Kim, Jong-Man,Kim, Jae-Myung,Nam, Hyang-Mi,Jung, Suk-Chan,Paik, Hyun-Dong Korean Society for Food Science of Animal Resource 2008 한국축산식품학회지 Vol.28 No.4
Lactococcus lactis NK34, isolated from jeotgal (Korean traditional fermented fish), produces bacteriocin against bovine mastitis pathogens such as Staphylococcus aureus 7, S. aureus 8, Staphylococcus chromogenes 10, S. chromogenes 19, Staphylococcus hominis 9, Streptococcus uberis E290, Enterococcus faecium E372, Streptococcus agalactiae ATCC 13813, Pseudonocardia autotrophia KCTC 9455, and Staphylococcus simulans 78. Lacticin NK34 was inactivated by protease XIV but not by protease IX, protease XIII, proteinase K, $\acute{a}$-chymotrypsin, trypsin, and pepsin. Also, lacticin NK34 was stable over a pH range of 2 to 9 for 4 hr and withstood exposure to temperatures of 30-$100^{\circ}C$ for 30 min. Lacticin NK34 showed bactericidal effects against S. simulans 78. This bacteriocin was purified using ammonium sulfate precipitation, ion exchange chromatography, ultrafiltration, and hydrophobic chromatography. Tricin-SDS-PAGE of purified bacteriocin gave the same molecular weight (3.5 kDa) as nisin. The gene encoding this bacteriocin was amplified by PCR using nisin gene-specific primers. It showed similar sequences to this nisin Z gene. These results indicate that lacticin NK34 is a nisin-like bacteriocin, and could be used as an antimicrobial alternative for livestock.
여러 분리원으로부터 유방염 원인균에 대한 항균력을 가진 유산균의 분리
이나경,최인애,박용호,김종만,김재명,정석찬,백현동,Lee, Na-Kyoung,Choi, In-Ae,Park, Yong-Ho,Kim, Jong-Man,Kim, Jae-Myung,Jung, Suk-Chan,Paik, Hyun-Dong 한국축산식품학회 2007 한국축산식품학회지 Vol.27 No.4
본 연구에서는 원유와 사일리지, 전통발효식품 등으로부터 유방염 원인균에 대한 항균성을 가지는 유산균을 분리하였고, 이들 중 원유에서는 79, 91-3 균주와 전통발효식품에서는 SA131, NK24, NK34, 32, 44-1 균주, 사일리지에서는 253, 261, 262, 263, 265 균주, 닭분변에서 B32, C23 균주에서 유방염 원인균에 대한 비교적 높은 항균력을 확인할 수 있었다. 이를 통해 새로운 항생제 대체물질의 가능성을 확인할 수 있었다. Bovine mastitis is costly infectious disease of dairy cattle, being responsible for significant economic losses all over the world. Also, mastitis has troubled about resistance to antibiotics. The purpose of this study was to screen a novel antimicrobial strain from various sources (raw milk and feeds (from farm of Paju, Dangjin, and Hwasung), commercial milk, Korean traditional fermented foods, and chicken feces). The isolate was screened using triple agar layer method and deferred method was used for confirmation of antimicrobial effect. Seventy six of isolates were screened using triple agar layer method. In these strain, 42 isolates were shown a broad spectrum of autimicrobial activity against mastitis pathogens. Especially, fourteen isolates were shown over 20 mm inhibition zone against S. aureous ATCC 25923. These results suggest that these novel antimicrobial strains could be used for the alternative of antibiotics.
Ovotransferrin 가수분해물의 Angiotensin-converting Enzyme 활성억제 효과 및 생산 최적화
이나경,안동욱,박근규,백현동,Lee, Na-Kyoung,Ahn, Dong-Uk,Park, Keun-Kyu,Paik, Hyun-Dong 한국축산식품학회 2010 한국축산식품학회지 Vol.30 No.2
Angiotensin-converting enzyme (ACE) inhibitory activity and production optimization of ovotransferrin hydrolysates were studied. Ovotransferrin was hydrolyzed by several enzymes (protamex, alcalase, trypsin, pepsin, neutrase, and flavorzyme) and acid (0.03 N HCl). Ovotransferrin hydrolysate reduced ACE activity by 60.2%, 55.8%, and 42.6% when treated with trypsin, acid, and pepsin, respectively. Trypsin was selected for production of peptide having maximum AC inhibitory effect, which was greatest with 7 h hydrolysis. Central composite design determined that optimum composition of ACE inhibitory substances using substrate concentration of 20-35%, temperature of $35-55^{\circ}C$, and pH of 6.0-8.0. The optimum composition was 1% trypsin, substrate concentration of 26.32%, $51.29^{\circ}C$, and pH 6.32. Under this conditions, a maximum ACE inhibitory effect of 69.1% was evident, similar to the predicted value.
경구투여한 V. vulnificus 백신의 면역원성 및 감염방어효능
이나경(Na Gyong Lee),정상보(Sang Bo Jung),안보영(Bo Young Ahn),김영지(Young Gi Kim),이윤하(Youn Ha Lee),전영중(Young Joong Jeon),박완제(Wan Je Park) 한국응용약물학회 1998 Biomolecules & Therapeutics(구 응용약물학회지) Vol.6 No.2
Vibrion vulnificus is an estuarine gram0negative human pathogen that affects people with chronic hepatitis, alcoholic cirrhosis, diabetes mellitus or other underlying diseases, V. vulnificus infection is mediated primarily by consumption of raw fish or by exposure of pre-existing wounds to seawater, causing permanent tissue damages or fatal septic shock. We have been developing a vaccine against V. vulnificus composed of whole cell lysate of a V. vulnificus O-antigen serotype 4 strain. Oral administration of the V. vulnificus vaccine elicited a high serum antibody response in rabbits. The induced antibodies were reactive not only to the homologous strain but also to heterologous O-antigen serotype strains, indicating cross-reactivities among serotypes. Western blot analysis revealed that the antibodies are mainly specific for outer membrane proteins (OMPs) and reacted equally well with OPMs purified from 9 O-antigen serotypes The rabbit antisera showed opsonophagocytic killing activity against heterologous strains as well as the homologous strain. Passively transferred rabbit antisera into mice were protective against a lethal V. vulnificus infection. These data demonstrate that oral administration of the V. vulnificus infections, suggesting that this vaccine preparation could be used to develop an oral vaccine against V. vulnificus. Keywords □ V. vulnificus. oral vaccine, immunogenicity, protective efficacy