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돼지 회충의 Sorbitol Dehydrogenase 에 관하여
채규주,이희성,이근배 ( Kyu Ju Chai,Hi Sung Lee,Keun Bai Lee ) 생화학분자생물학회 1978 BMB Reports Vol.11 No.4
The distribution of sorbitol dehydrogenase activity was studied in muscle, intestine and ovary of Ascaris strum. The tissues were fractionated by the method of Schneider and Hogeboom and the enzyme activity was measured using Gerlach and Hiby`s procedure. The results obtained were as follows: 1. The enzyme activity intrude preparations of muscle, intestine and ovary was found to be 1,152 units, 2,647 units and 3,366 units per g wet tissue, respectively. 2. Muscle showed the lowest enzyme activity among the tissues tested. Sventy-eight per cent of the activity was present in the cytosolic fraction(912 units/g wet weight). Twenty per cent of the activity (235 units/g wet tissue) was recovered in the mitochondria) fraction. 3. In the intestine, the activity was twice higher than that of muscle and 72% of the total activity was found in cytosolic fraction. 4. Ascaris ovary had the highest enzyme activity and contained 92% of the activity in the cytosolic fraction (3,400 units/g wet tissue).
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돼지 회충의 Sorbitol Dehydrogenase에 관하여
채규주,이희성,이근배,Chai, Kyu-Ju,Lee, Hi-Sung,Lee, Keun-Bai 생화학분자생물학회 1978 한국생화학회지 Vol.11 No.4
돼지회충(Ascaris suum)의 근육, 장관 및 난소조직을 분별 원심분리하여 핵, mitochondria 및 세포질을 분리하였으며, NAD 의존성인 sorbitol dehydrogenase의 활성도를 Gerlach and Hiby(1974)의 방법으로 측정하여 이 효소의 존재 유무 및 분포를 관찰하여 다음과 같은 결과를 얻었다. 1. 돼지회충의 근육, 장관 및 난소등의 조직 1g당 이 효소의 활성도는 각각 1152 unit, 2647 unit, 및 3666 unit 이다. 2. 근육조직의 세포질에는 79%인 912unit, mitochondria에는 20%인 235 unit 가 함유되어 있으며 비활성도는 3.84 및 3.56으로 작용능력은 거의 같다. 3. 장관조직에는 세포질에 72%인 1921 unit, mitochondria에는 13%인 352 unit 및 핵분획에 14%인 388 unit가 각각 함유되어 있다. 4. 난소조직에 이 효소가 가장 많이 함유되에 있으며, 92%인 3400 unit가 세포질에 함유되어 있으며 비활성도가 16.7로 이 효소의 작용능력이 다른 조직에 비하여 가장 활발하다. The distribution of sorbitol dehydrogenase activity was studied in muscle, intestine and ovary of Ascaris strum. The tissues were fractionated by the method of Schneider and Hogeboom and the enzyme activity was measured using Gerlach and Hiby's procedure. The results obtained were as follows: 1. The enzyme activity intrude preparations of muscle, intestine and ovary was found to be 1,152 units, 2,647 units and 3,366 units per g wet tissue, respectively. 2. Muscle showed the lowest enzyme activity among the tissues tested. Sventy-eight per cent of the activity was present in the cytosolic fraction(912 units/g wet weight). Twenty per cent of the activity (235 units/g wet tissue) was recovered in the mitochondria) fraction. 3. In the intestine, the activity was twice higher than that of muscle and 72% of the total activity was found in cytosolic fraction. 4. Ascaris ovary had the highest enzyme activity and contained 92% of the activity in the cytosolic fraction (3,400 units/g wet tissue).
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