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한국산 고등균류에 관한 연구(제 5보) -능이 중 단백분해효소의 특성과 N-말단 아미노산배열-
은재순,양재헌,이태규,최동성,Eun, Jae-Soon,Yang, Jae-Hean,Lee, Tae-Kyu,Choi, Dong-Seong 대한약학회 1989 약학회지 Vol.33 No.6
The alkaline protease produced by Sarcodon aspratus(Berk) S. Ito. was purified from its fruit bodies. The enzyme was purified by using ammonium sulfate fractionation, tris-acryl CM-cellulose column chromtography and chromatofocusing. The protease migrated as one major band with a molecular weight of about 29,000 dalton on sodium dodecylsulfate-polyacrylamide gel electrophoresis. The amino acid sequence of the N-terminal residues(21) of the enzyme was determined by automated sequence analysis. The sequence was Val-Thr-Thr-Lys-Gln-Thr-Asn-Ala-Pro-Trp-Gly-Leu-Gly-Asn-Ile-Ser-Thr-Thr-Asn-Lys-Leu. Comparison of this sequence with the N-terminal sequence of the p-roteinase K from Tritirachium album showed high similarity, i. e. 57.8% identical residues. The protease displayed a relatively high stability in sodium dodecyl sulfate.
은재순,염정렬,오석흥,권진,강성룡,오찬호,소준노,전훈,Eun, Jae-Soon,Yum, Jeong-Yul,Oh, Suk-Heung,Kweon, Jin,Kang, Sung-Young,Oh, Chan-Ho,So, Joon-No,Jeon, Hoon 대한약학회 1995 약학회지 Vol.39 No.5
The purpose of this research was to investigate effects of glycyrrhizin on the differentiation of preadipocytes, 3T3-Ll cells and to characterize the action of glycyrrhizin that affect the responses of 3T3-Ll cells during differentiation. The differentiation of 3T3-Ll cells was stimulated by glycyrrhizin, and triglyceride contents was increased in the differentiated 3T3-LI cell extracts. Total protein contents was increased by glycyrrhizin or inductive agents in the differentiated 3T3-Ll cell extracts. Calmodulin contents was increased by inductive agents, but the contents was not affected by glycyrrhizin in the differentiated 3T3-Ll cell extracts. The results suggest that glycyrrhizin has a stimulating activity of adipose conversion, but the activity is not related to calmodulin contents during the process of differentiation of 3T3-LI cells.
은재순,염정열,Eun, Jae-Soon,Yum, Jung-Yul 한국생약학회 1998 생약학회지 Vol.29 No.3
The oral administration of Aurantii nobilis pericarpium (ANP) extract and Aurantii immaturi pericarpium (AIP) extract suppressed the cell viability of both thymocytes and splenocytes in BALB/c mice. The ANP extract (500 mg/kg) enhanced the population of $B220^+$ cells, and the AIP also enhanced the population of B220+ and Thy-1+ cells in splenocytes. The AIP extract enhanced the population of $CD4-CD8^+$ cells in splenic T-lymphocytes. However, the ANP did not affect, whereas the AIP enhanced the phagocytic activity and the nitric oxide production in peritoneal macrophages.
한국산 고등균류에 관한 연구(제4보)-송이엑스가 백서의 고지혈증에 미치는 영향-
은재순,양재헌,김대근,Eun, Jae-Soon,Yang, Jae-Heon,Kim, Dae-Geun 한국약제학회 1989 Journal of Pharmaceutical Investigation Vol.19 No.3
This study was attempted to investigate the effect of Tricholoma matsutake on experimentally induced hyperlipemia in rats. The levels of cholesterol, triglyceride and phospholipid were measured. Diagnostic attention has been paid to the cholesterol concentration associated with high density lipoprotein (HDL), which appears to be inversely related to the incidence of coronary artery disease. For these reasons, determination of HDL-cholesterol has significant meaning. Total cholesterol was determined by Abell-Kindall method, and to measure HDL-cholesterol, serum low density lipoproteins (LDL) were first selectively precipitated by HDL-precipitating reagent. The results obtained were as follows : 1) The level of total cholesterol in serum of hyperlipemic rats was decreased by oral administration of Tricholoma matsutake extract. 2) The level of HDL-cholesterol was increased by the mushroom extract. 3) The level of triglyceride was significantly decreased by the mushroom extract. 4) The level of phospholipid was slightly decreased by the mushroom extract.
암세포주에 대한 유근피 n-BuOH 분획과 항암제의 병용효과
은재순,송원영,Eun, Jae-Soon,Song, Won-Young 한국생약학회 1994 생약학회지 Vol.25 No.2
The combined effects of Ulmi Cortex and some anti-cancer drugs on the proliferation of HeLa cells, Hep G2 cells and S 180 cells were estimated by MTT calorimetric assay. The n-BuOH fraction(UBF) of Ulmi Cortex inhibited the proliferation of HeLa cell at $10^{-3}\;g/ml$, Hep G2 cell at $10^{-5}\;g/ml$ and S 180 cell at $10^{-3}\;g/ml$. The inhibitory effects of mitomycin C(MMC), cisplatin(CPT) and 5-fluorouracil (5-FU), respectively, on Hep G2 cell was increased by the UBF. The UBF did not influence the proliferation of Balb/c 3T3 cells at concentrations of $10^{-6}$ to $10^{-4}\;g/ml$, but increased the proliferation of T cells at concentrations of $10^{-5}$ to $10^{-4}\;g/ml$. The UBF did not influence the number of leukocyte, and on the thymus weight of mice. The UBF increased the number of total-peritoreal cells of mice. In conclusion, the results suggest that the UBF have anti-cancer activity without the side effect, such as leukopenia and immunosuppresion, and increase the inhibitory activity of the anti-cancer drugs on Hep G2 cells.
3T3-L1 세포(細胞)의 증식(增殖) 및 분화(分化)에 미치는 백복령(白茯笭), 택사(澤瀉) 및 창출(蒼朮)의 영향(影響)
은재순,홍종성,소준노,Eun, Jae-Soon,Hong, Jong-Sung,So, June-No 한국생약학회 1993 생약학회지 Vol.24 No.2
These studies were conducted to investigate the effects of the extracts from Hoelen alba, Alismatis Rhizoma and Atractylodes Rhizoma on the proliferation and differentiation of 3T3-L1 cells. The results were summerized as follows: Hoelen alba and Alismatis Rhizoma extracts inhibited the proliferation of preadipose 3T3-L1 cells. In inductive differentiation, all three extracts inhibited the adipose conversion in 2 days of initial-culture, Atractylodes Rhizoma extract inhibited the adipose conversion in 5 days of final-culture and Hoelen alba and Alismatis Rhizoma inhibited adipose conversion in treatment of whole term of culture. In spontaneous differentiation, Atractylodes Rhizoma extract increased the adipose conversion in 2 days of initial-culture, Hoelen alba and Alismatis Rhizoma increased the adipose conversion in 5 days of final-culture, all three extracts increased adipose conversion in treatment of whole term of culture. The 10% serum of mice treated with each sample did not affect, but the 5% serum of them inhibited the proliferation of 3T3-L1 cells. In inductive differentiation, the 10% serum of them inhibited the adipose conversion in treatment of whole term of culture.
섬유아세포(纖維芽細胞)(Balb/c 3T3)의 증식(增殖)에 미치는 신효탁리산(神效托裡散)의 영향(影響)
은재순,전용근,염정열,서은실,소준노,오찬호,Eun, Jae-Soon,Jeon, Young-Keun,Yum, Jeong -Yul,Suh, Eun-Sil,So, June-No,Oh, Chan- Ho 한국생약학회 1993 생약학회지 Vol.24 No.2
The studies were conducted to investigate the effect of Sinhyo-Taklee-San(STS), which is composed of Astragali Radix(AR), Lonicerae Flos(LF), Angelicae gigantis Radix(AGR) and Glycyrrhizae Radix(GR), on the proliferation of fibroblast cell(Balb/c 3T3). STS, GR and glycyrrhizin increased the proliferation of 3T3 cells. The 10% serum obtained from STS, AR, LF, AGR and GR treated mice also increased the proliferation of 3T3 cells markedly. GR, glycyrrhizin and glycyrrhetinic acid inhibited protein synthesis, but did not affect on DNA synthesis.
한국산 고등균류에 관한 연구(제 1보) -능이버섯의 단백분해효소 활성-
은재순,양재헌,조덕이,이태규,Eun, Jae-Soon,Yang, Jae-Heon,Cho, Duck-Yee,Lee, Tae-Kyu 한국약제학회 1988 Journal of Pharmaceutical Investigation Vol.18 No.3
This study was undertaken to investigate the proteolytic enzyme from Neungee mushroom [Sarcodon aspratus (Berk) S. Ito]. The proteolytic activity of Neungee was higher than other several edible mushrooms under various pHs. The potency of proteolytic enzyme of Neungee was same as the digestive drugs containing protease. So the proteolytic activity of the enzyme was increased in neutral or weak alkaline pH, whose characteristics would be alkaline protease. The specific activity of the purified enzyme obtained by using Tris acryl CM-cellulose ion exchange increased 20 times as compared with that of the crude extract. The proteolytic enzyme was stable at room temperature, but decomposition was fast when incubated at higher temperature more than $40^{\circ}C$. The half life of the enzyme was longest in neutral pH and rate constant was increased in acidic or alkaline solution.