家蠶의 Vitellogenin에 관한 硏究

尹馨珠,李相夢,文在裕 한국잠사학회 1987 한국잠사곤충학회지 Vol.29 No.1

In the hemolymph proteins of the silkworm reared with mulberry leaves and artifcial diet, electrophoretic patterns of Storege Protein 1 (SP1) and Vitellogenin(VG), changes of relative concentration of SP1, JHA effects during the developmental stages, and estimation of the molecular weight of vitellin subunits were investigated in the present study. 1. Storage Protein 1 (SP1) showed female specificity from the middle stage of the fifth instar to the end of spinning stage in the silkworm reared with mulberry leaves and artificial diet. 2. Vitellogenin (VG) showed female specificity just after pupation in the silkworm reared with mulberry leaves and artificial diet. 3. Storage Protein 2 (SP2) without female specificity was detected from the early stage of the fifth instar to the first or second day after pupation. 4. Vitellin was composed of two non-identical subunits (vitellin·heavy chain; VTL-H, vitellinlight chain; VTL-L) with molecular weight of 186,000 and 41,000. Also, there were no differences between the molecular weights of vitellin subunits obtained from silkworms reared with mulberry leaves and artificial diet. 5. Relative concentration of Storage Protein 1 (SP1) after topical application - 0, 5, and 10μg per body weight - of JHA on the 60th hour of the fifth instar showed the highest increase with the treatment of 5μg and a higher increase with the treatment of 10μg compared with the control(no topical application of JHA).

연소도이득을 적용한 핵임계안전성평가를 위한 Depletion 계산코드의 검증

윤형주,홍서기,박광헌 한국방사성폐기물학회 2015 한국방사성폐기물학회 학술논문요약집 Vol.2015 No.05

천잠알의 수시부화 및 최청법 개선

윤형주,강필돈,이상몽,김삼은,김기영,Yoon, Hyung-Joo,Kang, Pil-Don,Lee, Sang-Mong,Kim, Sam-Eun,Kim, Ki-Young 한국응용곤충학회 2007 한국응용곤충학회지 Vol.46 No.2

We investigated starvation of hatching larvae, occasional artificial hatching and incubation method to establish year-round rearing of the wild silkmoth, Antheraea yamamai. In the test of starvation of hatching larvae for brushing at a time, the survival rate of the fourth instar of larvae starved for 1 day after hatching in $25^{\circ}C\;and\;5^{\circ}C$ was 83.3% and 96.0%, respectively. The result represents that the survival rate is high at low temperature during starvation. In the occasional artificial hatching test for multi-times rearing of A. yamamai, the useful hatchability is high at $5^{\circ}C$ in case of preserving eggs for 2 months from incubation time, and at both $2.5^{\circ}C\;and\;0^{\circ}C$ in case of over 6 months. A new incubation method with pre-incubation at $15^{\circ}C$ and 24 D photoperiod showed high hatchability about 80% for only 2 days compared with hatching for 5-6 days in traditional incubation method with the preservation at $25^{\circ}C$. 천잠의 연중 실내사육법을 확립하고자 천잠의 의잠절식, 수시부화 및 최청법을 조사하였다. 천잠의 일시소잠을 위하여 의잠을 절식 실험한 결과, $25^{\circ}C$ 와 $5^{\circ}C$ 에서 1일간 보호한 4령기잠기 생존율이 각각 83.3% 및 96.0% 로 의잠 절식기간의 보호온도가 낮을수록 생존율이 높았다. 연중 다회사육을 위해 수시부화실험을 한 결과, 천잠알을 2개월 냉장할 경우에는 $5^{\circ}C$가 좋았으나 6개월 이상 냉장하여 사용할 경우에는 $2.5^{\circ}C$또는 $0^{\circ}C$에 보호하는 것이 실용부화비율이 높은 것으로 나타났다. 또한 일시부화에 적합한 천잠알의 보호조건을 조사한 결과, 관행적 최청방법 ($25^{\circ}C$ 보호)으로는 5-6일 걸리는 천잠알의 부화가 $15^{\circ}C$ 예비최청과 암최청 방법의 도입으로 2일간에 80% 가 부화하는 좋은 성적을 얻을 수 있었다.

FRAPCON 3.5 코드를 이용한 PWR 고연소도사용후핵연료의 열화거동 평가

윤형주,박광헌 한국방사성폐기물학회 2015 한국방사성폐기물학회 학술논문요약집 Vol.2015 No.10

실내 인공사료육에 의한 뽕나무하늘소(Apriona germari Hope) 유충의 발육

윤형주,박인균,마영일,설광열 한국응용곤충학회 1997 한국응용곤충학회지 Vol.36 No.4

실내 인공사료육에 의한 뽕나무하늘소(Apriona germari Hope) 유충의 발육특성을 조사하기 위하여 야외 뽕밭에서 채집된 뽕나무하늘소 부화유충을 14L:10D 광조건의 $25^{\circ}C$ 항온기에서 애누에용 인공사료와 뽕나무 가지 분말을 동량비로 석은 인공사료에 의하여 실내사육한 결과, 유충의 1령에서 12령까지의 두폭 범위는 0.12~0.69 cm 내에 있었고 각 영기의 성장비는 1령과 2령간이 가장 컸다. 체중의 증가에 있어서 1령에 비해 8령의 경우는 약 176배, 12령의 경우와는 약 354배의 성장도 차이를 보였다. 각 영별 경과일구는 영이 진전될수록 경과일수가 길어졌으며 1령에서서 9령까지의 경과일수는 186.03일, 12령까지의 경과일수는 304.58일이었다. 인공사료육에 의한 1세대 유충 생존율은 3령기에 걸쳐 약 45%로 매우 낮았으나 그 이후 안정되어 8령까지의 유충 생존율은 40.8%이었다. 용화율은 32.4% 이었으며, 용화 시기는 7령에서부터 11령에 걸쳐 나타났지만, 8령과 9령째가 대부분이었다. Hatched-larvae of the mulberry longicorn beetle, Apriona germari Hope, collected from mulberry fields were reared on artificial diet at 25$^{\circ}$C with 14 h light and 10 h dark to study the larval developmental characteristics. Artificial diet developed for rearing silkworm was used with minor modification adding mulberry branch powder. In case of artificial diet rearing, the head width of larval instar from the I st to the 12th instars was ranged from 0.12 to 0.69cm, and growth rate of each instar was significantly high between the I st and the 2nd instars. In addition, the weight of the 8th instar larvae was increased approximately 176-fold in comparision with that of the 1st instar larvae. Larval duration of each instar took long with larval developmental stages, and that of the 1st to the 9th or the 12th instars was 186.03 or 304.58 days, respectively. The survival rate of larvae was 40.8% by the 8th instar. The pupation rate was approximately 32.4%. Furthermore, although pupation stage was broadly appeared from the 7th to the I lth instars, pupation was majorly observed at the 8th and the 9th instars.

왕지네(Scolopendra subspinipes) 먹이 및 활동시간

윤형주,이경용,이영보,신민지,김선영 한국응용곤충학회 2016 한국응용곤충학회 학술대회논문집 Vol.2016 No.10

왕지네는 전통 생약중 하나로 유효물질 탐색 및 약리활성 연구 등의 주요한 생물자원으로 한방에서 중풍, 해독, 관절염 등의 민간 약제로 이용되고 있다. 이에 왕지네를 실내 사육하기 위하여 먹이 및 활동특성을 조사하였다. 먹이를 갈색거저리구, 닭고기구, 돼지고기구, 닭고기+돼지고기 혼합구, 닭고기+ 돼지고기+갈색거저리 혼합구로 나누어서 실험한 결과, 성충 1마리당 먹이량, 체중증가량 등으로 볼 때, 갈색거저리보다 닭고기, 돼지고기, 닭고기+돼지 고기 혼합구의 발육이 우수하여 왕지네 먹이로는 닭고기, 돼지고기 등이 적합한 것으로 생각된다. 또한 카메라 영상장치를 이용하여 1개월 동안 왕지네의 활동시간대를 조사한 결과, 분산유충은 낮 12시에 가장 활발하고 먹이활동 은 오후 4시-8시 사이에 이루어졌다. 성충은 활동 시간대가 저녁 9시-새벽 6시이었고, 새벽 0시-2시가 가장 활발하였다. 먹이활동은 오후 9시-새벽 2시에 먹는 것으로 나타나, 지네 먹이를 줄 때는 가능한 저녁시간에 주는 것이 먹이의 신선도 유지와 부패를 막는 방법이라고 판단된다.

복소변수를 이용한 스핀 로켓의 자세 제어 기법

윤형주 空軍士官學校 1997 論文集 Vol.39 No.-

The thrust misalignment of sounding rockets can cause the attitude errors and make the system unstable. Especially, since the multi-staged rockets may have large attitude errors during the stage separation process, the attitude control system is indispensible. In the study, the longitudinal rolling spin is considered to reduce the effect of thrust misalignment. When a rocket has a rolling spin, the effect of misalignment can be averaged out. But the pitch and the yaw dynamics are cross coupled since rolling spin causes the gyroscopic effect. Therefore, the attitude dynamic system becomes MIMO(Multi Input, Multi Output) system.

Effect of Photoperiodic Regimes on Oviposition and Colony Development of the Bumblebee, Bombus ignitus

윤형주,김삼은 한국잠사학회 2003 International Journal of Industrial Entomology Vol.7 No.2

Oviposition and Colony Development of the Bumblebees, Bombus ignitus and B. terrestris depending on Different Pollen

윤형주,김삼은,이경용,이상범,박인균 한국잠사학회 2005 International Journal of Industrial Entomology Vol.11 No.2

We investigated oviposition and colony development of Bombus ignitus and B. terrestris depending on different pollen. In five kinds of pollen blend, the colony development in fresh-freezing pollen blend produced in Korea (Korea-FFP) was the best performance,although egg-laying characteristics is lower than that of pollen blend for oviposition imported from Korppert company (Kopport-FOP). The Kopport-FOP proved that it was suitable to use for oviposition of bumblebees. The Korea-FFDP, freezing dried-fresh pollen blend produced in Korea, is lower rather than the Korea-FDP although it is similar to the Kopport- FOP in colony development. It is not efficient to use commercial pollen for bumblebee because it is expensive in cost. The dried pollen blend for honeybee feeding imported from China (China-DP) was not suitable for rearing of bumblebee because it did not form colony although the worker emerged. In types of pollen,the oviposition and colony development of B. ignitus were not affected by the fresh-freezing pollen and dried-freezing pollen. This result also indicated that dried pollen, dried in the shade for 5 − 6 days, is possible to use as commercial pollen for bumblebee reproduction

Colony Developmental Characteristics of the Bumblebee Queen, Bombus ignitus by the First Oviposition Day

윤형주,이상범,김삼은,김영수 한국잠사학회 2004 International Journal of Industrial Entomology Vol.8 No.2

It was investigated whether developmental characteristics of foundation queens of Bombus ignitus collected in the 4 localities in Korea would be affected by the first oviposition days of them. The first ovipostion day was classified as 1 - 4 days (immediate early), 5 - 6 days (early), 7 - 10 days (delayed early), 11 - 20 days (medium), 21 - 40 days (late), and above 41 days (very late). The queen that had the early first oviposition day, i.e., laid eggs so early after starting to be raised indoors, showed much higher rate of colony foundation and progeny-queen production and much shorter period of colony foundation and worker emergence. Besides, the numbers of worker and progeny-queen emerged from the queen that had the early first oviposition day were higher than those of the queen that had the late first oviposition. In results, the queen that had the early first oviposition day could make colony stronger and could make colony formation period shorter, therefore, the first oviposition day of foundation queen was proved to be a criterion for the selection of super colonies when B. ignitus is raised indoors.