고농도 포도당에 노출된 INS-1세포와 백서 췌도 세포에서 포도당 산화 및 Reactive Oxygen Species (ROS) 생성

윤지성,원규장,이형우 대한당뇨병학회 2006 Diabetes and Metabolism Journal Vol.30 No.4

-Background: Chronic exposure of pancreatic islets to supraphysiologic concentrations of glucose causes beta cell dysfunction that is a process known as glucose toxicity. It has been reported that hyperglycemia increases the production of reactive oxygen species (ROS) in human islets and that ROS accumulation causes beta cell dysfunction associated with low capacity of intrinsic antioxidant enzymes. Also it has been postulated that this increase in ROS is prevented by an inhibitor of electron transport chain complex. The purpose of this study were to determine whether prolonged exposure of pancreatic islets to supraphysiologic glucose concentrations disrupts the intracellular balance between ROS thereby causing defective insulin secretion and to evaluate the site of hyperglycemia- induced ROS production.Methods: INS-1 cells & rat islets were incubated in increasing concentrations of glucose and either an inhibitor of complex I & II (TTFA), an uncoupler of oxidative phosphorylation (CCCP), aCCA, etc and also incubated in increasing concentration of glyceraldehyde and N-acetylcystein. Then intracellular peroxide levels by flow cytometric analysis and glucose induced insulin secretion were detected. Results: We observed that incubation with 30 mM glucose increased intracellular peroxide levels but decreased glucose-stimulated insulin secretion (GSIS) (P < 0.05). Exposure to TTFA, CCCP, aCCA did not reduce this increased intracellular peroxide levels, and did not increase GSIS (P < 0.05). 24-h incubation with glyceraldehyde at 5.6 mM glucose increased intracellular peroxide levels and decreased insulin content. Conclusion: These observations indicate that there might be other origins in which ROS species are produced besides electron transport chain in mitochondria and glyceraldehyde may be a key molecule to produce ROS, and induce beta cell dysfunction. (J Kor Diabetes Assoc 30:246~253, 2006)

Understanding the Cardiovascular Effects of Incretin

윤지성,이형우 대한당뇨병학회 2011 Diabetes and Metabolism Journal Vol.35 No.5

Cardiovascular disease (CVD), a leading cause of death in patients with diabetes mellitus, has several pathogenic mechanisms that are well established. However, the traditional hypoglycemic agents do not have proven positive effects on macrovascular disease. Novel therapeutic agents target the incretin pathway including the glucagon-like peptide 1 (GLP-1) receptor (GLP-1R) agonists and the dipeptidyl peptidase-4 inhibitors. The glucose-regulatory actions of these agents function by increasing insulin secretion and suppressing glucagon. They also act to increase weight loss not only by inhibiting gastric emptying, but also by reducing appetite. Although GLP-1 and GLP-1R agonists have demonstrated beneficial effects on myocardium and vascular endothelium including coronary and peripheral mouse vessels, they also have anti-inflammatory and anti-atherogenic actions. These agents also have positive effects on the lipid profile and blood pressure. Although these cardioprotective actions seem to be beyond the effects of glucose control and weight loss, they are mediated through GLP-1R- or GLP-1R-independent actions of cleaved GLP-1 (9-36). Larger randomized controlled trials are necessary to elucidate the clinical promise of these beneficial CVD effects.

체외수정시술시 난포자극호르몬 수용체 유전자 다형성이 과배란유도 및 임신 결과에 미치는 영향

윤지성,최영민,임경실,허창영,강영제,정재훈,이원돈,임진호,황규리,지병철,구승엽,서창석,김석현,김정구,문신용,Yoon, Ji-Sung,Choi, Young-Min,Lim, Kyung-Sil,Hur, Chang-Young,Kang, Young-Je,Jung, Jae-Hoon,Lee, Won-Don,Lim, Jin-Ho,Hwang, Kyu-Ri,Jee, Byun 대한생식의학회 2004 Clinical and Experimental Reproductive Medicine Vol.31 No.2

Objective: To investigate the association of FSH receptor (FSHR) polymorphism at position 680 with outcomes of controlled ovarian hyper-stimulation for IVF-ET in Korean women. Design: Genetic polymorphism analysis. Materials and Methods: The FSHR polymorphism was analyzed by PCR-RFLP in 172 ovulatory women below the age of 40 year. Patients with polycystic ovary syndrome, endometriosis, or previous history of ovarian surgery were excluded. Results: Genotype distribution was 41.9% for the Asn/Asn, 47.7% for the Asn/Ser, and 10.5% for the Ser/Ser FSHR genotype group. There was no difference in age of subjects and infertility diagnosis between genotype groups. When the patients were grouped according to their FSHR genotype, the basal levels of FSH (day 3) were significantly different among the three groups ($6.0{\pm}0.3\;IU/L$ (mean $\pm$ SEM), $5.8{\pm}0.3\;IU/L$, and $8.6{\pm}1.2\;IU/L$ for the Asn/Asn, Asn/Ser, and Ser/Ser groups, respectively, p=0.002). The Ser/Ser group showed a higher total doses of gonadotropins required to achieve ovulation induction, and a lower serum estradiol levels at the time of hCG administration compared with other two groups, but the differences were of no statistical significance. The numbers of oocytes retrieved were significantly different among the three groups ($8.6{\pm}0.8$, $9.9{\pm}0.6$, and $6.3{\pm}0.9$, for the Asn/Asn, Asn/Ser, and Ser/Ser groups, respectively, p=0.049). Clinical pregnancy rates were 42.4%, 25.9%, and 29.4% for the Asn/Asn, Asn/Ser, and Ser/Ser groups, respectively. Conclusion: Homozygous Ser/Ser genotype of FSHR polymorphism at position 680 was associated with decreased ovarian response to gonadotropin stimulation for IVF-ET.

Dendrimer를 이용한 금 표면 위의 단백질 고정화 연구

윤지성,강정식,박진호,김재호 한국공업화학회 2003 한국공업화학회 연구논문 초록집 Vol.2003 No.-

우리 교회 명교사를 소개합니다 : 김혜란 선생님

윤지성 장로회신학대학교 기독교교육연구원 2010 교육교회 Vol.393 No.-

지역주의 투표는 변화했는가? : 제14~20대 대통령 선거를 중심으로

윤지성 경북대학교 사회과학기초자료연구소 2023 연구방법논총 Vol.8 No.1

This study verifies the changes in regional voting over the 30 years since democratization, from the 14th presidential election in 1992 to the 20th presidential election in 2022, by using cross-pressure standard scores through time-series comparison. Demographic factors that influenced vote choices were age and residential area, and it was confirmed that regional cleavages were reduced from the Honam and Yeongnam regions to the Honam and Daegu-Gyeongbuk regions. In addition, the voting propensity of voters in Busan-Ulsan-Gyeongnam region is not different from that of voters in regions other than Yeongnam and Honam regions, suggesting that they are in a very flexible state. 본 연구는 1992년 제14대 대통령 선거부터 2022년 제20대 대통령 선거까지 민주화 이후 30년동안 나타난 지역주의 투표의 변화 양상을 교차압력 표준점수를 사용하여 시계열 비교로 검증한다. 유권자들의 투표선택에 영향을 미친 인구사회학적 요소는 연령과 거주지역이었으며, 지역 균열은 호남지역과 영남지역에서 호남지역과 대구·경북 지역으로 축소되었음을 확인했다. 또한 부산·울산·경남 지역 유권자들의 투표성향은 영호남 이외 지역 유권자들의 그것과 차이가 없어져서 매우 유동적인 상태임을 시사한다.

The Glucotoxicity Protecting Effect of Ezetimibe in Pancreatic Beta Cells via Inhibition of CD36

윤지성,문준성,김용운,원규장,이형우 대한의학회 2016 Journal of Korean medical science Vol.31 No.4

Inhibition of CD36, a fatty acid transporter, has been reported to prevent glucotoxicity and ameliorate high glucose induced beta cell dysfunction. Ezetimibe is a selective cholesterol absorption inhibitor that blocks Niemann Pick C1-like 1 protein, but may exert its effect through suppression of CD36. We attempted to clarify the beneficial effect of ezetimibe on insulin secreting cells and to determine whether this effect is related to change of CD36 expression. mRNA expression of insulin and CD36, intracellular peroxide level and glucose stimulated insulin secretion (GSIS) under normal (5.6 mM) or high glucose (30 mM) condition in INS-1 cells and primary rat islet cells were compared. Changes of the aforementioned factors with treatment with ezetimibe (20 μM) under normal or high glucose condition were also assessed. mRNA expression of insulin was decreased with high glucose, which was reversed by ezetimibe in both INS-1 cells and primary rat islets. CD36 mRNA expression was increased with high glucose, but decreased by ezetimibe in INS-1 cells and primary rat islets. Three-day treatment with high glucose resulted in an increase in intracellular peroxide level; however, it was decreased by treatment with ezetimibe. Decrease in GSIS by three-day treatment with high glucose was reversed by ezetimibe. Palmitate uptake following exposure to high glucose conditions for three days was significantly elevated, which was reversed by ezetimibe in INS-1 cells. Ezetimibe may prevent glucotoxicity in pancreatic β-cells through a decrease in fatty acid influx via inhibition of CD36.

Diabetogenic Effect of Statins: A Double-Edged Sword?

윤지성,이형우 대한당뇨병학회 2013 Diabetes and Metabolism Journal Vol.37 No.6

Statins are widely prescribed cholesterol-lowering agents, which have been demonstrated to significantly reduce cardiovascular morbidity and mortality. However, recent trials have reported that statins cause worsening of hyperglycemia and increase the risk of new-onset diabetes. The association between the diabetogenic effect of statins with intensive dose and accompanying major risk factors for diabetes has been demonstrated. However, statins do not appear to have a class effect on insulin sensitivity in non-diabetic patients. Numerous mechanisms have been suggested to explain how statins cause β-cell insulin secretory dysfunction and peripheral insulin resistance leading to incident diabetes. According to findings from an aggregate of large clinical trials,the benefits of statin treatment appear to outweigh the risk of new-onset diabetes. Therefore, it would be inappropriate to discontinue the use of statins for prevention of cardiovascular events because of its potential risk for development of incident diabetes. This review addresses the currently available evidence related to statin use and new-onset diabetes from a clinical perspective.

자폐성장애 중·고등학생 자녀를 둔 부모의 양육 스트레스 연구

윤지성,정은희 한국특수아동학회 2017 특수아동교육연구 Vol.19 No.2

Purpose: This research was to investigate the parenting stress of the parents of autistic children attending to middle and high schools. Method: Total number of 114 parents were tested who has middle and high school students with Autism Spectrum Disorder in a special schools and special classes in Gyeonggido. One-way ANOVA and t-test were used. Result: First, the parenting stress was slightly lower than the middle level. Second, the parents of male students were more stressful than those of female students, and the stress of parents with middle school students was higher than that of parents with high school students. In addition, as a child's disability grade was higher, the higher the stress was in adaptation, acceptance, demand, and mood. In the parental domain, the higher the degree of disability, the higher the stress on attachment, role limitation, competence, and isolation. The parenting stress of the parents was generally lower when their children were in an integrated education environment. Third, in terms of parental gender, mothers' stress was higher than father's in mood, and distractibility/hyperactivity and the latter was higher than the former in attachment and isolation. The parents group of college graduates and the less than middle school graduates had higher stress than that of high school graduates. Conclusion: In order to reduce the parenting stress of children with autistic disorder, family support is needed considering the characteristics of each life cycle, sex, and degree of disability. Support for adolescent problem behavior management is particularly needed for families with adolescent children. 연구목적: 본 연구는 자폐성장애 중․고등학생 자녀를 둔 부모의 양육 스트레스를 알아보고자 한 것이다. 연구방법: 이를 위하여 경기도지역 특수학교와 특수학급의 자폐성장애 중․고등학생 자녀를 둔 부모 114명을 대상으로 설문을 실시하고 수집된 자료는 t검정, 일원배치분산분석을 사용하여 처리하였다. 연구결과: 첫째, 부모의 양육 스트레스는 중간 수준보다 다소 낮은 정도였다. 둘째, 자녀의 특성에 따른 부모의 양육 스트레스를 알아본 결과 남학생의 부모가 여학생의 부모보다 스트레스가 더 높았고, 고등학생보다 중학생을 둔 부모의 스트레스가 높았다. 또한 자녀의 장애등급이 높을수록 적응, 수용, 요구, 기분 영역에서 스트레스가 높게 나타났으며 자녀가 통합교육 환경인 경우에 양육 스트레스가 더 낮았다. 부모영역에서는 장애등급이 높을수록 애착, 역할제한, 유능감, 고립에서 높은 스트레스를 보였다. 셋째, 양육자별로 볼 때 기분, 주의산만/과잉행동에서 아버지보다 어머니의 스트레스가 더 높았고, 애착과 고립에서는 어머니보다 아버지의 스트레스가 더 높게 나타났다. 또한 대졸과 중졸 이하 집단이 고졸집단보다 스트레스가 더 높았다. 결론: 자폐성장애 자녀의 양육스트레스를 감소시키기 위해서는 생애주기별, 성별, 장애정도별 특성을 고려한 가족지원이 필요하다. 특히 청소년기 자녀를 둔 가족에게는 사춘기 문제행동 관리 방법에 대한 지원이 필요하다.

패션 컬렉션에 표현된 헤어스타일과 의상의 부조적 장식기법에 관한 연구

윤지성 한국피부미용향장학회 2010 한국피부미용향장학회지 Vol.5 No.3