액상 정액의 보존 기간이 정자 기능 및 정자염색질 구조 분석에 미치는 영향과 인공수정 분만율과의 상관관계

유재원 ( J. W. Ryu ),이주형 ( J. H. Lee ),김인철 ( I. C. Kim ),이일주 ( I. J. Lee ),강권 ( K. Kang ),민동수 ( D. S. Min ),윤희진 ( H. J. Yun ),윤종택 ( J. T. Yoon ),방명걸 ( M. G. Pang ),류범용 ( B. Y. Ryu ),정영채 ( Y. C. Chung ),김 한국동물자원과학회 2006 한국축산학회지 Vol.48 No.6

체외수정 및 미세조작에 의한 가축배의 생산과 효율적 이용에 관한 연구 6. 소 핵이식배의 체외배양 , 동결보존 및 성판정에 관한 연구

정영채,김창근,윤종택,나광빈,오성종,이종완,김흥률,김광식,박선애,유영아 ( Y . C . Chung,C . K . Kim,J . T . Yoon,G . B . Luo,S . J . Oh,J . W . Lee,H . R . Kim,K . S . Kim,S . A . Park,Y . A . You ) 한국축산학회 1997 한국축산학회지 Vol.39 No.3

This study was carried out to establish an effective method for the improvement of subsequent development after nuclear transfer(NT) in bovine embryo, for the extension of utility through freezing and sexing of NT embryos. The rates of cleaved and developed embryos upto morula-blastocyst stages after the NT in in vitro produced embryos were 60.6 and 3.7%, respectively. Whereas, in in vivo embryos, slightly higher developments were obtained(70.8 and 5.6%), respectively. No difference was found in embryonic development upto morulablastocyst stages among the different levels of EGF added to the serum containing medium. The rates of cleaved and developed embryos upto morula-blastocyst were higher when electrostimulation was performed either before fusion or after addition at 21h IVM. The survival rate of frozen NT embryos was higher in late morula than that of earlier embryos. PCR-based sexing of NT embryo demonstrated that higher proportion of embryos was male(1.9:1), instead of normal ratio(1:1).

한국재래산양에서 계절이 정소기능, 정자의 내동성 및 수정능력에 미치는 영향 I. 정액성상과 정자의 내동성 및 난자침입능력의 계절적 변화

김창근,정영채,김광식,윤종택,이장희,정영호,최선호,김흥률,김수,권처진,Kim, C.K.,Chung, Y.C.,Kim, K.S.,Yoon, J.T.,Lee, J.H.,Chung, Y.H.,Choi, S.H.,Kim, H.Y.,Kim, S.,Kwon, C.J. 한국동물번식학회 1994 Reproductive & developmental biology Vol.17 No.4

This study was conducted to observe seasonal and individual changes in semen characteristics and sperm freezability, and sperm penetration into zona-free hamster eggs in Korean native goats. Buck response and change in semen characteristics to electrical stimulations was evaluated for four seasons throughout 2 years and percentage of motile sperm and normal apical ridge acrosome was investigated after equilibration and thawing for 4 seasons with 5 bucks. Sperm penetration rate was evaluated for 4 bucks. 1. Probe insertion at depth of 7cm and repeated stimulation for 3 sec was more effective(P<0.05) in buck response and semen collection than those of other conditions. 2. Semen characteristics from electrojaculation was signficantly(P<0.005) higher in spring and fall for semen volume, in spring and summer for sperm concentration and in fall for sperm motility than those in other seasons, respectively. However, there were no differences in total sperm among seasons. 3. Buck response to electrical stimulation showed significant difference(P<0.05) among individuals in all 3 seasons except winter. Significant individual difference in semen volume was only in spring and summer, but there was no indivudual difference in sperm concentration and total sperm in all season. 4. Washing of semen before freezing treatment was greatly(P<0.05) beneficial to sperm motility after thawing, no matter whether ejaculates exhibit egg yolk coagulation or not. 5. Sperm motility after glycerol equilibration was significantly(P<0.05) low in summer semen and motility after thawing was greatly(P<0.05) higher in winter semen than in other seasons. Freezability of unwashed sperm was significantly difference among bucks, but a yearly freezability of washed sperm after chilling and thawing were no differences among bucks and percentage of normal apical ridge acrosome were not different among seasons and bucks. 6. There was no significant difference in sperm motility after thawing between egg yolk levels in summer, although 20% level gave more higher motility than 5% level. 7. In summer, 3.2% glycerol and 3-h equilibration gave greatest percentage(P<0.05) of sperm motility and normal apical ridge acrosome after thawing. 8. Sperm penetration rate into zona-free hamster eggs was not different between bucks and seasons. Overall, it is concluded that to obtain maximum sperm output and successive semen freezing by electrojaculation method, buck selection with good response in all season could be basically considered and that seasonal effect on sperm freezability was more greater than that of individual bucks.

반추가축 (反芻家畜) 조사료 이용성 증진을 위한 한국 재래 산양 섬유소 분해 효소에 관한 연구

장문백(M . B . Chang),맹원재(W . J . Maeng),민병열(B . R . Min),이상석(S . S . Lee),윤종택(J . T . Yoon) 한국영양사료학회 1994 韓國營養飼料學會誌 Vol.18 No.4

한국재래산양에서의 계절이 정소기능, 정자의 내동성 및 수정능력에 미치는 영향 II. 혈청내 Testosterone수준의 계절 및 하루중 변화

김창근,정영채,김광식,김수,권처진,한기영,윤종택,정영호,이장희,Kim, C.K.,Chung, Y.C.,Kim, K.S.,Kim, S.,Kwon, C.J.,Han, K.Y.,Yoon, J.T.,Chung, Y.H.,Lee, J.H. 한국동물번식학회 1994 Reproductive & developmental biology Vol.17 No.4

Seasonal and diurnal variation in serum testosterone was measured by radioimmunossary in 3 mature Korean native male goats. Blood was collected at 3-h intervals for 24h in each season. Mean levels of 4 seasons ranged from 0.82 to 2.06ng/ml and no difference was among seasons, although level in fall and winter was slightly higher. Diurnal level tended to increase at daytime in all season and when diurnal variation in level was compared to daily mean of each season, level of daytime greatly(P<0.05) increased in summer. However, variation between light and dark period in other seasons did not show significant difference. Daytime increase of testosterone was found 3 to 4 after dawn and level increased 1 to 2h ahead in summer and fall than that in others. These results in serum testosterone suggest that there was no great seasonal variation in endocrine function of goat testis.

미경산돈에서 교배전 자궁내 정액항원 주입에 의한 산자수 증가에 관한 연구

한기영(K . Y . Han),김창근(C . K . Kim),윤종택(J . T . Yoon),김형태(H . T . Kim),정영채(Y . C . Chung),서경덕(K . D . Seo),연정웅(J . W . Youn),이용우(Y . W . Lee) 한국축산학회 1988 한국축산학회지 Vol.30 No.9

This experiment was conducted to evaluate effect of a single intrauterine infusion of semen or seminal plasma at estrus before mating on subsequent litter traits of crossbred gilts. In addition, presence and level of early pregnancy factor activity (rosette inhibition titre, RITZ in serum of treated gifts and in vitro immunosuppressive activity of these antigens from boars used were tested immunologically to support the results in this experiment. The results obtained were as follows: 1. Semen-infused gifts significantly had a larger litter size (11.4) at birth than controls (9.6), but no significant differences were detected between others. 2. There was a tendency toward increased total pigs born alive, litter weight at birth and number of pigs suckling in antigen-infused gifts, but the differences between treated-gifts and controls were not statistically significant. 3. EPF activity (RIT) before and after mating in previously infused-gifts was 10.7±2.8 in the serum of nonpregnant gifts and 19.3±3.8 in the serum of pregnant gifts. RIT $gt; 14 could be considered evidence of presence of EPF in pregnant gifts. 4. Seminal plasma infusion before mating induced on obvious rise of RIT. RIT at 24 h and 48 h after mating was much higher (18-23 and 21-22) :in semen-and seminal plasma-infused gifts than in controls. RIT at 24 h after mating was found to be positively correlated (r=0.84) to litter size born in 6 gifts. 5. Dialyzed seminal plasma from 4 boars was significantly immunosuppressive at a concentration of 500 ㎍/㎖ than controls and degree of immunosuppression varied among individuals when the concentrations was under 500 ㎍/㎖. Undialyzed semen samples from 3 boars resulted in loss of immunosuppressive activity. These results indicate that infusion of gifts with semen at estrus before mating can be improved reproductive efficiency because of more litter size at birth and sukling pigs than controls and that these antigens had immunosuppressive potential of antilymphocyte serum and immunosuppressive activity to porcine lymphocytes that generate an immune response.

소의 체외성숙난포란의 체외수정과 발생에 관한 연구

김창근(C . K . Kim),정영채(Y . C . Chung),윤종택(J . T . Yoon),최선하(S . H . Choi),류범용(B . Y . Ryu),정광조(K . J . Chung),김흥율(H . R . Kim),송해범(H . B . Song) 한국축산학회 1990 한국축산학회지 Vol.32 No.1

Effects of adding 10 to 20% fetal calf serum(FCS) and estrous cow serum(ECS), and hormones(GTH or GTH plus E₂) to maturation medium on in vitro maturation(IVM) and in vitro fertilization(IVF) of bovine follicular oocytes and effects of sperm treatment and bull on IVF and embryo development were studied. Finally, developmental potential these in-vitro fertilized embryos at 2-to 8-cell stage(44h postinsemination) was investigated under in vivo(rabbit oviduct) and in vitro(Ham`s Fl0 plus 10% FCS with or without bovine oviduct epithelial cells, BOEC) 4 day-culture system. 1. Addition of 10 to l5% FCS to mKRB and DM(-BSA) and of 20% FCS to Ham`s F10 increased(65%) IVM, respectively. IVM of these media supplemented with hormones was higher(70∼72%) than that of media without hormone. Addition of ECS showed higher IVM at 10% level for mKRB and 10 to 15% for TCM199 and the highest IVM(88%) was obtained in TCM199 containing 10% ECS and LH. 2. Higher IVF rate (based on formation of both pronuclei) in Exp I was obtained from oocytes matured in mKRB plus 10% FCS and HIS-sperm treatment. In Exp 2. IVFrate (based on cleavage. 2-3 cells) of oocytess matured in TCM199 was higher than in other media and IVF using fresh semen was significantly higher but was similar between sperm treatments. In Exr 3, IVM in TCM199 containing 10% ECS and LH and IVF by C+H-sperm treatment significantly increased early cleavage(2-to 8-cell). 3. In vitro-acrosome reaction(AR) among individual bulls in Exp 1 ranged from 16 to 45% and bull group having lower AR showed low IVF rate than group having higher AR. In Exp 3, cleavage rate by sperm treatment with both chemicals(C+H) was higher than that by H alone. 4. In IVF with in vivo-capaciated sperm, development from 2-to 3-cell to above 8-cell was higher under in vivo culture(54.5%) than under in vitro culture(31.3%) and higher in oocytes matured in TCM199 plus to ECS. In IVF with in vitro-capacitated sperm, development into above 8-cell stage was different between initial stage(2-to 3-and 4-to 8-cell) of embryos(26.7% : 41.7%) and significantly lower under in vitro culture from 2-to 3 cell stages(l0% : 29.4%). Under in vitro co-culture with BOEC, embryo development from 4-to 8-cell was significantly improved(53.3%) and 3(20%) of 15 cultured embryos developed into morula. The present results indicate that addition of ECS and hormones can improve IVM, IVF and development of bovine follicular oocytes, sperm factor may be important for IVF and further development, and co-culture with BOEC is an effective means for development into above 16-cell stage.

韓國在來山羊 反芻胃內 Cellulolytic Bacteria의 酸素活性에 關한 硏究

尹鍾澤,裵貴石,孟元在,張文伯,李相碩 中央大學校 食糧資源硏究所 1993 食糧資源硏究所 論文集 Vol.5 No.1

本 實驗은 反芻胃微生物의 纖維素 分解作用 대한 基礎資料를 얻기 위해 韓國在來山羊 反芻胃內 纖維素分解微生物을 分離·同定하여 醱酵産物, 電子顯微鏡的 檢定을 실시하였다. 그 結果는 다음과 같다. 1. pH水準은 醱酵期間동안 反芻胃 最適 範圍인 6.52∼6.68으로 正常範委로 維持되었다. 2. 보리짚을 substrate로 한 乾物 消化率은 公培養에서 높앗다. 3. 微生物 乾物生成量을 Mixed group에서 낮은 傾向을 보였다. 4. NDF 消化率은 monoculture구에서 높은 結果를 나타내었으며 72時間 培養시 coculture구에서 높은 結果를 보였다. 5. Ammonia-N 濃度는 72시간까지 漸次的으로 증가하는 傾向이었으며, coculture group에서는 48時間帶에 다소 낮아졌다. 6. 전자현미경적 관찰을 통해 분해가 활발히 일어남을 보여 주었다. The isolated ruminal celluloytic bacteria in the rumen of Korean native goat, Fibrobacter succinogenes, Butyrivibrio fibrisolvens, Ruminococcus fibrisolvens were incubated on barley straw in mono-and coculture. The microbes were examined for its ability to degrade barely straw and compared with synergy between mono- and coculture. This experiment was conducted to study the characteristics of predominant cellulolytic bacteria in the rumen of Korean Native Goat on the regulation of enzyme activiy, and undertaking to isolate and identification the bacterial flora and to determine fermentation factor, degradation of fiber source. The results obtained in this study are as follows: 1. Morphology in the light microscope, they are grown on defined substrates and no difference was observed. 2. pH level was maintained with cellulolytic ruminal optimum range (6.52∼6.68) during fermentation period. 3. Dry matter digestibility was the highest for tri-culture with barely straw substrate. In the case of Mixed group, similiar digestibility were observed. 4. NDF digestibility was the highest for B. fibrisolvens but the substrate was more rapidly de-graded after 48 h by F. succinogenes. 5. Nicrobial dry matter yield of coculture were lower than monoculture group. 6. The coculture of these microorganisms on barely straw was less effective than monocultures in concentration of ammonia nitrogen. 7. Observations by scanning electron microscopy showed that barely straw were degraded by microbes.

逆流浸降 電流法에 依한 돼지의 X-, Y-精子分離 關한 硏究

李用斌,呂政秀,鄭用基,尹種擇,徐國聖 서울大學校 農科大學 1980 서울대농학연구지 Vol.5 No.2

This experiment was carried out to separate X- and Y- bearing sperm of boar semen from 4 boars raising at the College of Agriculture, Seoul National University. The boar semen collected was treated by the counter streaming sedimentation and galvanization. The results obtained were as follows; Average appearance of B-body from control was 45.2%, when the experiment was performed with counter streaming sedimentation alone appearance of B-body from top and bottom fraction were 59.4% and 36.3%, respectively. The frequency of appearance of B-body from 30(24%) and 40(19.6%) minutes among treatments were statistically significant differences. Appearance of B-body from anode was 54% and from cathode was 37.9% after counter streaming sedimentation and galvanization. The difference between anode and cathode was significant differences at the 30(14.7%), 40(19.4%) and 50(19.9%) minutes. The frequency of appearance of B-body from counter streaming galvanization was significantly higher than that from counter streaming sedimentation. Therefore, the result from counter streaming sedimentation(30 minutes) and galvanization(30 minutes) was the most useful.