간경변증(肝硬變症)의 치료(治療)

윤종만 대한소화기학회 1970 대한소화기학회지 Vol.2 No.2

Genetic Distances between Two Echiuran Populations Discriminated by PCR

윤종만 한국발생생물학회 2019 발생과 생식 Vol.23 No.4

Genomic DNA extracted from representatives of two populations, Gunsan and Chinese, of Urechis spp. was amplified using PCR with several primers. The band-sharing (BS) value between individuals no. 05 from the Gunsan population and no. 22 from the Chinese population was 0.206, which was the lowest recognized value. Oligonucleotides primer OPC-04 revealed 44 unique loci, which distinguished the Chinese population. Primer OPB-17 allowed the discovery of 22 loci shared by the two populations, which were present in all samples. Based on the average BS results, individuals from the Gunsan population demonstrated lower BS values (0.661±0.012) than did those from the Chinese population (0.788±0.014; p<0.05). The shortest genetic distance (GD) displaying a noteworthy molecular difference was between individuals CHINESE no. 12 and no. 13 (GD=0.027). Individual no. 06 from the Gunsan population was most distantly related to CHINESE no. 22 (GD=0.703). A group tree of the two populations was constructed by UPGMA Euclidean GD analysis based on a total of 543 fragments generated using six primers. The explicit markers recognized in this study will be used for genetic analysis, as well as to evaluate the species security and proliferation of echiuran individuals in intertidal regions of the Korean Peninsula.

틸라피아의 해수순치시(海水馴致時) 아가미와 신장(腎臟)의 광학(光學) 및 전자현미경적(電子顯微鏡的) 관찰(觀察)

윤종만,조강용,박홍양,Yoon, Jong-Man,Cho, Kang-Yong,Park, Hong-Yang 한국현미경학회 1993 Applied microscopy Vol.23 No.2

1990년(年) 6월(月) 30일(日)부터 10월(月) 31일(日)까지 또한 1991년(年) 9월(月) 30일(日)부터 1992년(年) 4월(月) 31일(日)까지 본 실험실의 양어장(養魚場)에서 염분(鹽分) 농도(濃度)가 각각 10%o, 20%o, 30%o의 $1.5m^3$인 탱크에서 사육된 100-350g(평균 250g)인 틸라피아(Oreochromis niloticus)의 아가미 및 신장(腎臟)의 미세구조적(微細構造的) 변화(變化)를 알기 위해서 광학현미경(光學顯微鏡)과 주사(走査) 및 투과전자현미경(透過電子顯微鏡)으로 관찰하여 얻어낸 결과는 다음과 같다. 본 연구에서 얻어진 결과는 다음과 같이 나타났다. 해수(海水)에 적응(適應)된 Oreochromis nilotirus의 경우 모두 아가미엽 비대, 아가미엽 상피세포의 분리, 신사구체의 수축, 신장(腎臟)의 울혈(鬱血) 등(等)의 조직학적(組織學的) 변화(變化)가 있었다. 가장 높은 저삼투압(低渗透壓) 능력(能力)은 10%o에서 채취된 틸라피아에서 확인되었고, 이러한 현상은 외형적인 형태를 통해서 확인된 염분순치화(鹽分馴致化)(smoltification)와 일치(一致)되었으며, 실험군 중 틸라피아 저삼투조절능력(低渗透調節能力)은 10%o에서 떨어지기 시작하였다. Oreochromis niloticus의 각 군의 삼투압(渗透壓)은 모두 담수(淡水)의 수준보다 높았고, 모두 300m Osmol 이상으로 나타났다. 아가미엽의 염분세포의 발생빈도와 그 정도는 염분농도(鹽分濃度)의 증가와 더불어 빠르게 증가되었고, 염분세포의 수도 광학(光學) 및 투과전자현미경(透過電子顯微鏡)을 통해서 볼 때 NaCl의 증가와 함께 증가하였다. 삼투압(渗透壓)의 점차적인 증가에 대해서 틸라피아의 체내에 축적된 NaCl과 무기성분(無機成分)을 배출시키기 위해서 혹은 체내의 삼투압(渗透壓)의 균형을 위해서 이러한 결과가 이루어지는 것으로 사료된다. 아가미엽(葉)의 표피(表皮)는 염분농도(鹽分濃度)가 높을수록 요철(凹凸)상태가 심하게 나타났다. 이러한 생리학적 현상들은 모든 경골어류(硬骨魚類)에서 일어나는 것으로 사료되며, 틸라피아와 무지개 송어(松魚)는 조직병리학적(組織病理學的) 변화(變化)에도 불구하고 해수(海水)에 견디어내어 순치(馴致) 적응(適應)하는 것으로 나타났다. 주사전자현미경(走査電子顯微鏡)에 의해서 해수(海水)에 적응(適應)된 틸라피아의 제 2차(次) 아가미엽(葉)의 미세구조(微細構造)는 적응기간(適應期間) 동안 표면이 울퉁불퉁한 특징을 나타내었고, 투과전자현미경(透過電子顯微鏡)에 의해서는 해수(海水)에 노출된 염분세포내(鹽分細胞內)에 길게 변형된 미토콘드리아가 관찰되었으며, 잘 발달된 cristae가 확인되었고, 담수(淡水)에 서식하던 개체의 염분세포(鹽分細胞)보다 그 수가 증가하였다. 이와같이 미토콘드리아가 풍부한 염분세포의 존재를 통해서 해수(海水)에 적응하는 동안 일어나는 저삼투압조절(低渗透壓調節) 작용(作用)에 중요한 역할을 하는 것이라 사료된다. 해수(海水)에 적응(適應)된 대부분의 틸라피아는 신장(腎臟)의 보우만 주머니를 가득 채운 신사구체(腎絲球體)를 가지고 있으며, 신사구체(腎絲球體)의 수축은 담수(淡水)에 적응된 개체보다 10%o, 20%o, 30%o에 적응된 개체에서 훨씬 더 많이 발생되었고, 울혈현상(鬱血現象)은 10%o보다 20%o, 30%o에 적응(適應)된 개체의 신장조직(腎臟組織)에서 더많이 발생되었다. 틸라피아의 신사구체(腎絲球體)는 담수(淡水)에서 10%o의 해수(海水)로 이주된지 14일(日) 이후에 신장(腎臟)에서 수축된 것으로 나타났다. 30%o의 해 This study was taken to examine the light microscopic and ultrastructural changes of gill and kidney of female tilapia{Oreochromis niloticus) adapted in 0%o, 10%o, 20%o, and 30%o salt concentrations, respectively, by light, scanning and transmission electron microscope. The results obtained in these experiments were summarized as follows: Gill chloride cell hyperplasia, gill lamellar epithelial separation, kidney glomerular shrinkage, blood congestion in kidneys and deposition of hyalin droplets in kidney glomeruli, tubules were the histological alterations in Oreochromis niloticus. Incidence and severity of gill chloride cell hyperplasia rapidly increased together with increase of salinity, and the number of chloride cells in gill lamellae rapidly increased in response to high external NaCl concentrations. The ultrastructure by scanning electron microscope(SEM) indicated that the gill secondary lamella of tilapia(Oreochromis niloticus) exposed to seawater, were characterized by rough convoluted surfaces during the adaptation. Transmission electron microscopy(TEM) indicated that mitochondria in chloride cells exposed to seawater, were both large and elongate and contained well-developed cristae. TEM also showed the increased chloride cells exposed to seawater. The presence of two mitochondria-rich cell types is discussed with regard to their possible role in the hypoosmoregulatory changes which occur during seawater-adaptation. Most Oreochromis niloticus adapted in seawater had an occasional glomerulus completely filling Bowman's capsule in kidney, and glomerular shrinkage was occurred higher in kidney tissues of individuals living in 10%o, 20%o, 30%o of seawater than in those living in 0%o of freshwater, and blood congestion was occurred severer in kidney tissues of individuals living 20%o, 30%o of seawater than in those living in 10%o of seawater. There were decreases in the glomerular area and the nuclear area in the main segments of the nephron, and that the nuclear areas of the nephron cells in seawater-adapted tilapia were of smaller size than those from freshwater-adapted fish. Our findings demonstrated that Oreochromis niloticus tolerated moderately saline environment and the increased body weight living in 30%o was relatively higher than that living in 10%o in spite of histopathological changes.

내시경 영역

윤종만 大韓消化器病學會 1988 大韓消化器病學會誌 Vol.20 No.3

韓國産 미꾸리에 關한 育種·繁殖學的 硏究 - Ⅳ. 卵母細胞의 卵黃 形成 및 成熟에 관한 電子顯微鏡的 觀察

尹鍾萬,李宗映,李慶鎬,朴弘陽 한국동물생명공학회(구 한국동물번식학회) 1992 Reproductive & developmental biology Vol.16 No.3

發達 段階에 따른 韓國産 메기(Silurus asotus) 卵母細胞의 微細構造的 變化

윤종만,김계웅,신호철,장계남,류동석,박홍양 한국동물생명공학회(구 한국동물번식학회) 1995 Reproductive & developmental biology Vol.19 No.2

Genetic Distances of Paralichthys olivaceus Populations Investigated by PCR

윤종만 한국발생생물학회 2018 발생과 생식 Vol.22 No.3

The author carried out PCR-based genetic platform to investigate the hierarchical polar dendrogram of Euclid-ean genetic distances of one bastard halibut population, particularly for Paralichthys olivaceus, which was further connected with those of the other fish population, by involving with the precisely designed oligonucleotide primer sets. Eight oligonu-cleotides primers were used generating excessively alterating fragments, ranging in size of DNA bands from larger than ap-proximately 100 bp to less than 2,000 bp. As regards average bandsharing value (BS) results, individuals from Hampyeong population (0.810) displayed lower bandsharing values than did individuals from Wando population (0.877). The genetic dis-tance between individuals approved the existence of close relationship in the cluster II. Relatively, individuals of one bastard halibut population were fairly related to that of the other fish population, as shown in the polar hierarchical dendrogram of Euclidean genetic distances. The points of a noteworthy genetic distance between two P. olivaceus populations demonstrated this PCR procedure is one of the quite a few means for individuals and/or populations biological DNA investigates, for spe-cies security and proliferation of bastard halibut individuals in coastal region of the Korea.

경골어류의 성전환에 관한 연구 II. $17\beta$-Estradiol 및 $17\alpha$-Methyltestosterone을 이용한 Guppy 및 Tilapia의 성전환에 관한 연구

윤종만,박홍양 한국동물번식학회 1989 Reproductive & developmental biology Vol.13 No.1

Guppy fry were treated for the first 40 days of life with 0, 20, 40, 60 & 100$\mu\textrm{g}$ of estradiol per gram of food in order to change the sex of normal males to functional females(genetic male). The present investigation deals with the effects of steroid hormones, such as $\beta$-estradiol and testosterone, on the sex differentiation in guppy and tilapia. The results obtained were summarized as follows. 1. In B (20$\mu\textrm{g}$/g diet) group 17$\beta$-estradiol-treated, 67.8% of male offsprings were produced. 2. In D (60$\mu\textrm{g}$/g diet) group 17$\beta$-treated, 67% of female offsprings were produced. 3. B, D groups of genetic male brooders had significantly different effects (P<0.01) upon sex ratios of their progeny. 4. This strongly indicates that sex direction has been achieved and that the male is the heterogametic sex. 5. The group that produced the highest percentage of male offspring(male percentage of observed number to expected number was 91%) contained only full-sibling male brooders to the sex-reversed female brooders. 6. After 7 months following treatment, the sex-reversed males had ovarian portion in the anterior region and a testicular portion in the posterior region of the same intersexual gonad, respectively. 7. At 7 months after treatment, the ovareis revealed a complete arrest of the ovarian formation, and appearances of spermatogenetic cell cysts among surviving auxocytes. 8. In most of sex-reversed fish, anterior portion of test is was devoid of sperm ducts including the seminal vesicle and vas deferens. 9. The male transferrin showed two strong bands, while the female transferrin showed a single weak band. 10. One of the two bands of male transferrin showed the same mobility with band of female transferrin.

연어(Oncorhynchus keta) 정자의 미세구조와 성분화

윤종만,오양수,김계웅,박홍양 한국동물생명공학회(구 한국동물번식학회) 1997 Reproductive & developmental biology Vol.21 No.3

무지개 松魚의 遺傳育種學的 硏究 - Ⅷ. 卵母細胞의 光學 및 微細構造的 變化

尹鍾萬,金映坤,朴弘陽 한국동물생명공학회(구 한국동물번식학회) 1991 Reproductive & developmental biology Vol.15 No.3