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우렝쉥이 껍질성분 및 색소를 이용한 양식소재 개발 - 2. 우렝쉥이 껍질 중 부분정제한 황산다당의 화학적 특성 -
홍병일(Byeong Il Hong),정병천(Byung Chun Jung),정우진(Woo Jin Jung),육지희(Ji Hee Ruck),최병대(Byeong Dae Choi),이강호(Kang Ho Lee) 한국수산과학회 2001 한국수산과학회지 Vol.34 No.6
우렁쉥이 껍질에서 분리한 황산다당을 부분정제하여 몇가지 화학조성과 전기영동을 하였다. 추출된 황산다당의 화학적 조성은 sulfate, uronic acid, protein, chondroitin sulfate, amino sugar, hexosamine 등으로 이루어져 있었다. 전기영동결과 autoclave 처리에 의해 추출된 조다당류는 폭넓은 단일 band를 나타내었으나, neutrase 처리구는 세 개의 band로 구분되었다. Autocalve 처리구의 분자량은 약 40,000 정도로 추정되며, neutrase 처리구의 경우 고분자 band는 100,000 이상, 두 개의 저분자 band 중 하나는 약 22,000 정도, 또 하나의 band는 약 5,000 정도인 것으로 추정되었다. 이온교환수지 및 겔 여과하여 얻은 전당획분의 화학적 조성은 부분정제 전과 거의 유사하였다. Sephadex G-25로 gel 여과한 시료의 조성당은 arabinose, xylose 및 glucose는 흔적량 정도이었으나 galactose는 71.4%로 대부분을 차지하였고, N-acetylgalactosamine 함량이 14.5% 정도이었고, D-glucuronic acid가 6.3% 정도 검출되었다. Sephadex G-100과 G-100-25로 정제한 당의 전기영동 패턴은 거의 유사하게 단일 band의 양상이나 G-25 처리구가 다소 좁은 band를 나타내었다. 부분정제한 황산다당과 표준품인 chondroitin sulfates의 Ff-IR spectrometer 분석 결과, 거의 유사한 결과를 나타내었다. 황산기나 에스테르 등 몇몇 중요한 원자단의 신축 진동수가 IR spectrum의 지문영역 (1,500∼700㎝^(-1)에서 나타났다. 황산기 중의 S=O 신축진동이 1,240 ㎝^(-1)에서, C-O-S 신축진동의 경우 수평향배는 820 ㎝^(-1)에서 나타났다. Components of polysaccharides isolated from ascidian tunic were measuerd by gel filtration, electrophoresis and chemical analyses. The sulfated polysaccharides consisted in sulfate, protein, uronic acid and amino sugars. Hexosamines were composed of arabinose, xylose, glucose, galactose, glucuronic acid, N-acetylgalactosamine and N-acetylglucosamine by gas chromatography analysis. The galactose was predominant hexose after autoclave and nutrase digestion followed by DEAE-cellulose ion exchange chromatography and gel-permeation chromatography on Sephadex G-100 and G-25. FT-IR spectra of isolated polysaccharides from ascidian tunic and standard chondroitin sulfates have similar functional groups of the type of vibration and frequency. Molecular weight of isolated polysaccharides by autoclave represented more than 40 kDa by polyacrylamide gel electrophoresis. But the neutrase treatment group divided into three band. The highest molecular band group was shown more than 100 kDa, and the two low molecular baud group were shown about 22 kDa and 5 kDa, respectively, compare to standard materials.
우렁쉥이 껍질성분 및 색소를 이용한 양식소재 개발 1 . 우렁쉥이 껍질 다당류의 추출방법
이강호(Kang Ho Lee),홍병일(Byeong Il Hong),최병대(Byeong Dae Choi),강석중(Seok Joong Kang),육지희(Ji Hee Ruck),정병천(Byung Chun Jung) 한국수산과학회 1998 한국수산과학회지 Vol.31 No.3
The effective extraction methods and chemical components of crude polysaccharides of ascidian tonics were investigated. Tew extraction conditions, autoclaving or enzyme treatment, were applied. The proximate composition of ascidian tunics was not much different between those dried in raw (containing pigments) and those acetone treated and dried (decolorized), showing 50% of carbohydrate and 40% of protein. It was possible to extract up to 10% of crude polysaccharides from ascidian tunics regardless of the extraction methods, autoclaving or enzyme treatment. In case of the latter the extraction yield by neutrase was higher than that with alkalase (Novo co.) or mixture 2000 (Pacific chemical co.), The mast effective enzyme concentration and extraction time appeared to be 24 hrs of extraction with 3% neutrase. On the other hand, in autoclave treatment, 6 hrs extraction showed mast desirable extraction yield, about 7.7%. The compositions of amino acid of decolorized ascidian tunic (acetone treated group) and the crude polysaccharide from the autoclaving (water solubles) or neutrase treatment (enzyme digestibles) were similar to each other. Histidine was the highest both in the neutrase and autoclave treatment group and the yield were 29.2%, 20.4%, respectively, followed by aspartic acid and glutamic acid. Among the minerals, the content of Ca was significantly high, followed by Mg and Na.
이강호(Kang Ho Lee),최병대(Byeong Dae Choi),홍병일(Byeong Il Hong),정병천(Byung Chun Jung),육지희(Ji Hee Ruck),정우진(Woo Jin Jung) 한국수산과학회 1998 한국수산과학회지 Vol.31 No.3
Functional properties such as anti-blood coagulation, angiotensin converting enzyme(ACE) inhibitory activity, fat binding capacity, foaming properties, emulsifying properties and chemical components of sulfated polysaccharides isolated from ascidian tunics were investigated. The sulfated polysaccharide mainly consisted of sulfate, uronic acid, protein, and chondroitin sulfate, among which chondroitin sulfate showed higher concentration while sulfate arid ironic acid did lower. Compositional monosaccharides were arabinose, xylose, glucose, galactose, glucuronic acid, N-acetylgalactosamine and N-acetyglucosamine. Especially, galactose content was dominant among them. And emulsifiability and foaminess of the sulfated polysaccharide was higher than the control group. Anti-blood coagulation of sulfated polysaccharide showed with respect to APTT (Activated partial thromboplastin time). ACE inhibitory activity showed about 16.7%,
우렁쉥이 껍질성분 및 색소를 이용한 양식소재 개발 - 3. 우렁쉥이 껍질 유래 황산다당의 기능적 특성
홍병일 ( Byeong Il Hong ),정병천 ( Byung Chun Jung ),손병일 ( Byung Yil Son ),정우진 ( Woo Jin Jung ),육지희 ( Ji Hee Ruck ),최병대 ( Byeong Dae Choi ),이강호 ( Kang Ho Lee ) 한국수산과학회 2002 한국수산과학회지 Vol.35 No.6
우렁쉥이 껍질에서 추출한 autoclave 추출구와 neutrase 추출구는 지방흡수력의 경우 대조시료인 chitin과 chitosan에 비해 떨어지는 대략 200~300% 정도이었으나, 포말성은 각각 0.70과 0.67로, 포말안정성은 0.50과 0.38로 우수하였다. Autoclave 추출구 및 neurase 추출구의 유화능은 각각 49.2%와 47.1%, 유화안정성의 경우 각각 48.3%와 44.8% 정도였으며, 특히 색소흡착능은 각각 85.2%와 85.6%로 대조시료에 비해 다소 우수하였다. 항혈액응고능은 모든 시료에서 APTT 저지효과는 나타났으나 PT값은 약 14.5초로 일정하여 혈액응고저지 효과가 거의 없었다. 특히 autoclave 처리구를 DEAE-cellulose ion exchange chromatography하여 얻은 F_4 (획분 4) 는 APTT가 75초로 가장 효과적이었으며, Sephadex G-100의 F_2가 70초 정도였다. ACE저해능은 모든 시료에서 ACE 저해효과가 있었으며, 채택된 실험방법에 따라 동일시료 경우에 있어서도 저해효과 값이 차이가 났으며, 특히 DEAE-cellulose 이온교환 처리한 F_4 (획분 4)가 35.75로 가장 효과적이었다. The present study was conducted to elucidate functional properties of sulfated polysaccharides from ascidian tunics. In physical properties of the crude polysaccharides, emulsion ability and foaminess were more excellent compared with chitin and chitosan, particular dye binding capacity was prominent. Anti-blood coagulation of partially purified sulfated polysaccharides showed with respect to APTT (Activated partial thromboplastin time). Especially, active fraction (F_4) obtained by DEAE-cellulose ion exchange chromatography showed highest activity, which was approximately 20% of the activity of heparin. ACE inhibitory activity also similar to anticoagulant activity. Active fraction (F_4) obtained by DEAE-cellulose ion exchange chromatography showed about 34%, in ACE inhibitory activity.