변계량 시의 변모와 그 문학사적 의미

유호진 ( Yu Ho Jin ) 한국시가학회 2003 韓國 詩歌硏究 Vol.14 No.-

오미자 리그난 화합물의 베타-아밀로이드간 결합 억제 및 해리 효과

유호진(Ho Jin Yu),윤미소(Mi So Yoon),김도윤(Do Yoon Kim),원경종(Kyung-Jong Won),김보경(Bokyung Kim),장상희(Sang-Hee Jang),이환명(Hwan Myung Lee) 대한약학회 2012 약학회지 Vol.56 No.5

The present study tested the effect of Schizandra chinensis lignan compounds, Gomisin A and Schizandrin, on the aggregation and dissociation of beta-amyloid (Aβ)1-42 to explore a possible therapeutic target for Alzheimer's disease. Gomisin A significantly inhibited the Aβ1-42 aggregation in a dose dependent manner, but did not induced the dissociation of aggregated Aβ1-42. On the other hand, Schizandrin significantly suppressed the aggregation and dissociation of Aβ1-42. These results suggest that Gomisin A and Schizandrin, which are known as biologically active ingredients from Schizandra chinensis, may be potentially useful target molecules to develop a drug for the prevention or treatment of Alzheimer's disease.

고려 후기 사대부 한시에 나타난 정신지향에 대한 연구 -최해, 안축, 이제현의 시문을 중심으로-

유호진 ( Yu Ho Jin ) 고려대학교 민족문화연구원 2003 민족문화연구 Vol.39 No.-

이달(李達) 시(詩)에 나타난 소멸(消滅)과 생성(生成)의 상상력에 대하여

유호진 ( Yu Ho-jin ) 한국시가학회 2020 韓國 詩歌硏究 Vol.51 No.-

Lee Dal(李達)'s poems have a couple of expressive features, including his frequent uses of depiction and often borrowing the narrative structure. The images of birds and insects, female figures, and fall that often appeared in his descriptions implied the poet himself gradually losing energy and vitality. He conveyed his awareness that his life force decreased little by little in the world of his sickness with a series of such images displaying his imagination of extinction. The poet began his inquiry into the essence of being as he developed a sense of emptiness in this awareness. In his works, the poet embodied plants with wounds to imply that the essence of being lied in the persistent will for life. Furthermore, he created a symbol of flower bundles to imply that life`s essence was the harmonious state of life force and beauty. Ideal figures that achieved the state were depicted in the images of generals, Taoist hermits with miraculous power, ascetics, hermits, and Yeins. This series of images that appeared in combination with the rise images attract attention as they show his imagination of creation. The poet paid attention to ordinary people who shared the same pain as him in his wandering life. He depicted ordinary people`s suffering in his poems in a short narrative structure, expressing his deep sympathy and compassion for them. Moreover, he also talked about his bond and consciousness of kind for them in his poems. He delivered the message of true happiness in life by describing the healthy and lively aspects of ordinary people. Lee Dal's poems imply a profound and broad spiritual world that he achieved by delving into the essence of being based on his hardship and exhibiting his spiritual journey of resonance with ordinary people`s pain and grief. He especially expressed his spiritual orientation with various images and colorful figures based on his imagination of extinction and creation, which is why his poetry in the Tang style(唐詩風) has been praised.

아라미드섬유를 적용한 철근콘크리트 구조물의 보수.보강 공법소개

황보석,노광근,유호진,Hwang, Bo-Seok,No, Gwang-Geun,Yu, Ho-Jin 한국건축구조기술사회 2008 건축구조 Vol.15 No.1

각질형성세포 유래 엑소좀이 피부각질형성세포의 증식과 이주에 미치는 영향

김도윤 ( Do Yoon Kim ),유호진 ( Ho Jin Yu ),황대일 ( Dae Il Hwang ),장상희 ( Sang Hee Jang ),이환명 ( Hwan Myung Lee ) 대한화장품학회 2016 대한화장품학회지 Vol.42 No.4

Exosome, a small vesicle secreted from cells, has diverse functions depending on cell origins and tissue types and plays a important role in cell viability and intercellular communication. Recently, many researchers have demonstrated the use of exosomes for the treatment of cancers and immune diseases, and the development of diagnostic biomarker. However, the secretion mechanism of exosome from skin cell and its physiological functions in skin remain unclear. Thus, this study aimed to explore whether keratinocyte-derived exosome affects proliferation and migration in HaCaTs. Exosomes were isolated from HaCaTs by ExoQuick-TC and then boiled or unbolied. Boiled and unboiled exosome induced proliferation in HaCaTs in a dose-dependant manner (0.1 ∼ 20 μg/mL), respectively. Boiled and unboiled exosome at concentration of 20 μg/mL increased proliferation level in HaCaTs by 186.96 ± 3.87% and 193.48 ± 10.48% compared with control group. Unboiled exosome stimulated migration in HaCaTs in a dose-dependent manner (0.1 ∼ 20 μg/mL), which reached a maxium at concentration of 20 μg/mL (179.39 ± 4.89% of control), but boiled exosome did not affect HaCaT migration. In addition, unboiled exosome (0.1 ∼ 20 μg/mL) dose-dependently stimulated sprout outgrowth in HaCats. These results demonstrate that in exosome from HaCaTs, heat-stable components such as lipid may induce HaCaT proliferation and heat-unstable components such as protein may stimulate migration and sprout outgrowth in HaCaTs, thereby leading to reepithelialization and skin-wound healing activities. It is concluded that exosomes from HaCaTs may be used as cosmetic materials.

Gomisin A Inhibits Tumor Growth and Metastasis through Suppression of Angiogenesis

Do-Yoon Kim(김도윤),Ho Jin Yu(유호진),Mi So Yoon(윤미소),Joo Hoon Park(박주훈),Sang-Hee Jang(장상희),Hwan Myung Lee(이환명) 한국생명과학회 2012 생명과학회지 Vol.22 No.9

항암화학요법제는 의약품 시장 중 가장 큰 시장의 하나이며, 세계적으로 수많은 연구와 신약의 개발이 진행되고 있다. 그러나 암 세포 뿐만 아니라 정상세포와 조직에 대한 독성과 내성으로 인해 심각한 부작용이 지속되어, 최근 신혈관형성 억제와 천연물 유래의 암 치료제 개발이 많은 관심을 얻고 있다. 본 연구에서는 오미자의 활성 성분 중 하나인 Gomisin A의 종양성장 및 전이억제 효과와 이들의 작용기전에 대해 확인하고자 하였다. Gomisin A의 종양성장 억제활성은 B16BL6 cell line을 C57/BL6 mice의 피하에 주입하여 유도된 종양모델을 활용 하였으며, Gomisin A 10 μg/ml, 100 μg/ml에서 양성 대조군에 비해 각각 80.5±8.1%, 96.2±2%의 유의한 억제활성을 나타내었다. Gomisin A의 암세포에 대한 직접적인 독성은 MTT assay를 통해 확인하였고, 정상세포(HUVECs)와 종양세포주(A549, B16BL6, Du145, Huh7)에 대한 유의한 독성은 관찰되지 않았다. 종양조직에 의해 유도되는 신혈관형성 억제능은 C57/BL6 mice에 배양된 B16BL6 cell line을 피하주사하여 종양조직을 형성하고, 이들이 유도하는 신생혈관에 대한 Gomsin A의 억제활성을 확인하였다. Gomisin A를 10 μg/head와 100 μg/ head에서 신혈관형성 억제활성은 양성 대조군에 비해 각각 151±16.9%, 98.5±29.5%의 유의한 감소활성을 나타내었다. 종양전이 억제활성은 B16BL6 cell line을 C57/BL6 mice 미정맥에 주입하여 in vivo 폐전이 모델을 만들고 폐에 전이된 종양의 colony를 측정하여 확인하였다. Gomisin A의 농도 10 μg/head, 100 μg/head에서 각각 양성 대조군에 비해 13.5±8.56%, 58.3±9.12%의 전이 억제활성을 나타내었다. 또한 Gomisin A는 B16BL6 cell line이 세포외기질에 대한 부착능을 유의하게 감소시킴을 확인하였다. 이러한 결과는 Gomisin A의 종양성장 억제활성이 암세포에 대한 직접적인 독성에 의한 것이 아닌 신혈관형성 억제에 의한 것이며, 또한 종양 전이 억제 활성을 나타냄으로서 향후, 암 예방 및 치료제 개발의 선도물질을 제공할 수 있음을 확인하였다. 본 연구는 오미자의 주요 생리활성 물질인 Gomisin A의 종양성장 억제 활성과 전이억제 활성을 규명하고자 하였다. Gomisin A가 종양성장 억제활성이 있음을 확인하였고, 이러한 효과는 종양세포에 대한 직접적인 독성에 의한 것이 아니라, 종양조직에 의해 유도되는 신혈관형성 저해 활성에 의해 나타나는 것을 규명하였다. 이러한 결과는 대부분의 항암화학요법제가 가지는 독성작용을 극복하고, 선택적인 종양성장 억제제로서의 개발 가능성을 시사하여 주고 있다. 또한 Gomisin A는 종양세포의 세포외기질 부착억제활성을 통해 암 전이 억제활성을 나타냄을 확인하였다. 지금까지의 결과는, Gomisin A를 활용한 부작용이 적으며 효과적인 암 치료제 개발의 선도 물질로서 활용 가능성을 나타내 주고 있으며, 또한 고 기능성 식품 및 화장품의 개발에 활용이 가능할 것이다. 그러나, 향후 종양 면역활성, 암세포 자살 유도능과 같은 다양한 부분의 연구가 수반되어야 할 것이다. Cancer chemotherapy drugs command a large share of the market, and the development of new therapeutics with high efficacy and specificity is an active area of study. Recently, the development of cancer therapeutics from natural products targeting angiogenesis has drawn attention due to conventional chemotherapeutics showing serious side effects and resistance in cancer cells. In this study, we investigated the pharmacological efficacy of Gomisin A, an active ingredient of Schizandra chinensis baillon, on tumor growth and metastasis. Administration of Gomisin A at 10 and 100 μg/ml reduced tumor growth in vivo by 80.5±8.1% and 96.2±2%, respectively, compared with positive tumor controls. Treatment of Gomisin A in normal and various tumor cell lines did not exert significant toxicity. Mice treated with Gomisin A at a concentration of 10 and 100 μg/head showed a significant reduction in tumor-induced angiogenesis of 151±16.9% and 98.5±29.5%, respectively. Furthermore, tumor metastasis analysis revealed that the administration of Gomisin A at a concentration of 10 and 100 μg/head inhibited tumor metastasis by 13.5±8.56% and 58.3±9.12%, respectively. In addition, Gomisin A significantly decreased cell adhesion of the B16BL6 cells to the extracellular matrix. These results demonstrate that Gomisin A inhibits tumor growth via suppression of angiogenesis and tumor metastasis inhibition, without cellular toxicity. The pharmacological efficacy of Gomisin A suggests that it may be a potential candidate for the development of cancer drugs.

저가형무인항공기를 이용한 문화재 촬영 및 등고선 제작

김지현(Kim, Ji Hyun),유호진(Yu, Ho Jin),정지원(Jung, Ji Won),김승현(Kim, Seung Hyeon),최현(Choi, Hyun) 한국측량학회 2018 한국측량학회 학술대회자료집 Vol.2018 No.4

지능정보기술 활성화를 위한 데이터 인프라 구축과 활용지원 모델

김원확(Kim Won Hwak),유호진(Yu Ho Jin),이은수(Lee Eun Soo) 한국통신학회 2017 한국통신학회 학술대회논문집 Vol.2017 No.6

Schizandrin의 신혈관형성억제에 의한 항암효과

윤미소(Mi So Yoon),김도윤(Do Yoon Kim),유호진(Ho Jin Yu),박주훈(Joo-Hoon Park),장상희(Sang Hee Jang),원경종(Kyung-Jong Won),김보경(Bokyung Kim),이환명(Hwan Myung Lee) 한의병리학회 2012 동의생리병리학회지 Vol.26 No.5

Schizandra chinensis extract has been known to possess a variety of efficacy including antitumor. However, it remains unclear how schizandrin, which is a major biological active ingredient of Schizandra chinensis, exerts antitumor effect. This study was designed to investigate the mechanism by which schizandrin inhibits tumor growth and metastasis. In in vivo test using tumor model mice injected with B16BL6 cell line, mice treated with 10 and 100 μg/ml schizandrin showed a significant inhibition by 73.79 ± 6.43% and 90.46 ± 1.72%, respectively, compared with positive tumor controls. Schizandrin did not exert a significant toxicity for the normal cells (HUVECs) and tumor cell lines (A549, B16BL6, Du145, Huh7). Treatment with schizandrin at 10 and 100 μg/head significantly inhibited the tumor-induced angiogenesis by 68.04 ± 32.21% and 103.8 ± 34.99% compared with the positive control group, respectively. Using in vivo lung metastasis model, tumor metastasis assay revealed that 10 and 100 μg/head schizandrin significantly decreased the metastatic lung tumor by 37.51 ± 8.15% and 75.53 ± 4.38% compared with positive controls, respectively. On the other hand, schizandrin did not affect the adherence of B16BL6 cell line to extracellular matrix protein. These results demonstrate that schizandrin exerts inhibitory effect on tumor growth and metastasis by inhibiting angiogenesis. This study thus suggest that schizandrin may be a candidate molecule target for cancer drug development.