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      • KCI등재

        동물 및 인체시험을 통한 Nattokinase의 항응고 작용 및 섬유소 용해능 평가

        김재범(Jae-Bum Kim),유철배(Chul-Bae Yoo),신현만(Hyun-Man Shin),정준기(Joon-Ki Jung),장형욱(Hyung-Wook Jang) 한국생물공학회 2011 KSBB Journal Vol.26 No.5

        Bacillus subtilis natto producing high level of a fibrinolytic enzyme was selected and Ultra Nattokinase<SUP>ⓡ</SUP> was manufactured by fermentation and purification. It was performed the evaluation of the antithrombotic effect of Ultra Nattokinase<SUP>ⓡ</SUP> (20,000 FU/g) with rat blood plasma. The maximum aggregation (inhibition ratio) was 71% (0%), 69% (2.8%), 62% (12.7%), 16% (77.5%) and 9% (87.3%), respectively, in the order of 0, 5, 10, 50 and 100 mg/mL of Ultra Nattokinase<SUP>ⓡ</SUP> solutions. Ultra Nattokinase<SUP>ⓡ</SUP> had antithrombotic effect, which was associated with the suppression of collageninduced platelet aggregation. Ultra Nattokinase<SUP>ⓡ</SUP> in the topic of the FDP (fibrinogen degradation products) in blood coagulation tests showed a significant increasing trend. And based on the daily record of meal 39 people of ITT (what ?) group consisted with 19 people of NP (what ?) group and 20 people of PN (what ?) group except four people, two people who took vitamin K affecting the experiment and two people who took alcohol, finding to be taken Ultra Nattokinase<SUP>ⓡ</SUP> showed an increase in the FDP value after four weeks. In addition, FDP value of 41 people of ITT group except two people having metabolic syndrome was increased by Ultra Nattokinase<SUP>ⓡ</SUP>.

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        Bacillus subtilis Natto가 생산하는 Nattokinase의 항혈전 및 피브린 용해능 효능평가

        이다령(Da-Lyung Lee),홍성유(Sung-Yu Hong),장양수(Yang-Su Jang),장형욱(Hyung-Wook Jang),맹창재(Chang-Jae Maeng),유철배(Chul-Bae Yoo),백대헌(Dae-Heoun Baek) 한국생물공학회 2012 KSBB Journal Vol.27 No.6

        We previously reported that Ultra nattokinase<SUP>ⓡ</SUP> showed high fibrinolytic activity and revealed antithrombotic effect in rat blood plasma based on its ability to suppress collagen-induced platelet aggregation. This research was carried out to verify the clot lysing activity and blood flow enhancing effects of Ultra nattokinase<SUP>ⓡ</SUP> via monitoring and comparing the antithrombotic effects in rat artery between oral administration of Ultra nattokinase<SUP>ⓡ</SUP> and maltodextrin. SD rats were fed with 1.11 mg/kg of Ultra nattokinase<SUP>ⓡ</SUP> for 4 weeks. The effect on arterial thrombosis was then evaluated using an antithrombotic model after induction by FeCl₃. Detected fibrinolytic activity was proportional to the content of Ultra nattokinase<SUP>ⓡ</SUP> and statistical extents of the antithrombotic activity was enhanced strongly twice rather than control group. The PT and the aPTT, however, showed only a small difference between two groups. The results suggest that Ultra nattokinase<SUP>ⓡ</SUP> can effectively treat thromboembolism and enhance blood flow, and that Ultra nattokinase<SUP>ⓡ</SUP> can also prevent venous occlusion by aiding clot lysis.

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