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소형 비닐하우스 내에서 훈연처리된 살균제 Procymidone 의 분포 특성
유주현,조광연,임희경,김정한 한국농화학회 2001 Applied Biological Chemistry (Appl Biol Chem) Vol.44 No.1
The distribution of procymidone in a small vinyl house containing cucumber plants or mimic plants was investigated after application of smoke generator. The deposition of procymidone on glass plate and filter paper was significantly similar to that on cucumber leaves, so that glass plate and filter paper could be substituted for cucumber leaves on deposition studies. The deposition of procymidone was proportional to the horizontally projected area of surface; the deposition on the horizontal surface was maximal, and the deposition was minimal for the perpendicular surface. The amount of deposition on the backside of leaf was less than 5% compared to that on the horizontal surface of leaf. The height of leaf from the floor was not a significant factor influencing on the deposition of procymidone whether leaves were overlapped or not. However, the deposition of procymidone on the overlapped leaves was relatively less than that on the unoverlapped leaves. And the deposition difference depending on the distance between leaves was not observable. Overall, the deposition of procymidone was proportionally increased with the application rate of smoke generator, but the deposition was inversely related to the sum of the total horizontal leaf area of mimic plants and the surface area of floor.
호알칼리성 Bacillus sp.가 생산되는 Bacteriolytic Enzyme을 이용한 Bacillus subtilis의 형질전환
유주현,이인숙,옥승호,박희경,염도영,배동훈 한국미생물 · 생명공학회 1993 한국미생물·생명공학회지 Vol.21 No.5
The extracellular bacteriolytic enzyme from alkalophilic Bacillus sp. YJ-451 was endopeptidase which hydrolyzes the peptide bond at the amino group of D-glutamic acid in the peptidoglycan. Protoplast transfomation system of B. subtilis by the lytic enzyme that differs, in mechanisms, from lysozyme which was used to transformation of B. subtilis was investigated. High protoplast yield was obtained from cells cultured in PAB at the late logarithmic growth phase.
유주현,조광연,이병회 한국농화학회 1992 Applied Biological Chemistry (Appl Biol Chem) Vol.35 No.2
The technicals of KC-7079, isoprothiolane, perfluidone and tricyclazole were granulated with a mixture of inorganic carrier and oil-soluble binder, that is, stearly alcohol or ethyl cellulose. The concentration of the released active ingredient from the granules was analyzed at several days intervals after immersion of these granules in water at 25℃. At the content of stearyl alcohol less than 80g kg^(-1), the granule kneaded with stearyl alcohol mixture and water disintegrated in water. But the granule kneaded with methanol disintegrated in water at the content of stearyl alcohol less than 30g kg^(-1). The less the KC-7079-stearyl alcohol granule disintegrated, the slower the release rate of KC-7079 was. No matter how was increased the stearyl alcohol content, the release rate of KC-7079 granule which did not disintegrate was not significantly changed. The sustained releasing effect of the granules was little in the other three pesticides of which the water solubility was higher than of KC-7079(21 ppm). The granule made of ethyl cellulose did not disintegrate even at 5g ㎏^(-1) of ethyl cellulose. With the increase of ethyl cellulose content and the decrease of active ingredient in the granules, the sustaining effect of the granules on releasing acitive ingredient was increased. The lower the water solubility of pesticide was, the release rate tended to be sustained except perfluidone.
99mTc-Liposomes 과 킬레이트제 표지반응의 효과
유주현,유용운,강태웅,김장휘 대한핵의학회 1985 핵의학 분자영상 Vol.19 No.1
Using chelating agents such as Nitrilotriaceticacid (N'rA), Diethylenetriaminepentaaceticacid (DTPA) and Ethylenediaminenitrilotetraceticacid (EDTA), effects of liposomes labelled with Tc- 99m were determined in vitro and in vivo. Methods of separation and determination of Tc-99m- liposomes added chelating agents were practiced by thin layer chromatogram scan and gel filtration. Biodistributions of Tc-99m-liposomes in normal and sarcoma 180 cells bearing mice were observed. The results were as follows: 1) Maximum amount of Sni+2 to reduction from pertechnetium (10∼20 μci) by adding 0, 1, 10 and 100 μg of SnC12 in 0.2 ml of oxygen free water was 10 μg. 2) The large amounts of SnC12 were not changed but the small amounts of SnC12 were much changed by labeling with Tc-99m to add chelating agents. EDTA in small amounts of SnC12 were reduced more strongly than DTPA or NTA. Using a hydrophilic chelate, DTPA, the uptake of liposomes could not accumulated in liver and spleen but a lipophilic chelate NTA were signant in vivo. 3) UPtake by tumor was achived 1.14% of injected dose per gram tissue and tumor to organ ratios were measured in low with Tc-99m-NTA-liposomes(+).