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RISS 인기검색어
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- 저자 : 유영아(You, Young Ah) (검색결과 4 건)
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정자 운동성 및 수명 보존을 위한 최적 배양에 관한 연구
유영아,오신애,방명걸,You, Young-Ah,Mohamed, E.A.,Oh, Shin-Ae,Pang, Myung-Geol 대한생식의학회 2009 Clinical and Experimental Reproductive Medicine Vol.36 No.1
목 적: 본 연구는 정상 정액을 배양액으로 세척한 후 $4^{\circ}C$, $22^{\circ}C$, $37^{\circ}C$에서 5일 동안 보존하면서 정자의 운동성, 생존성을 관찰하여 정자의 운동성과 수명 유지를 위한 적정 배양 환경을 분석하고자 하였다. 연구방법: 정액검사 시 정상으로 판정된 남성의 정자를 HTALP 배양액으로 세척하여 정자의 최종 농도 $1{\times}10^6/ml$로 각 5 ml을 배양온도 $4^{\circ}C$, $22^{\circ}C$, $37^{\circ}C$에서 5일 동안 배양하였다. 1일, 3일 5일째에 CASA에 의해 운동성을 측정하였고, HOST로 정자막의 온전성을 분석하였으며, CTC pattern으로 수정능획득 상태 분석하여 최적의 배양 환경을 분석하였다. 결 과: 정자의 운동성, 생존성 및 수정능획득이 야기되지 않은 정자는 배양일에 따라 유의하게 감소하였다 (p<0.05). 또한 정자 배양 후 1일에는 정자의 운동성, 생존성 및 정자막의 온전성과 CTC pattern은 온도에 따라 차이가 없었으나, 배양 후 3일과 5일에서는 $22^{\circ}C$에서 배양된 정자가 다른 배양온도에 비해 가장 잘 보존되었다 (p<0.05). 결 론: HTALP로 세척된 정자를 $22^{\circ}C$에서 보존 시 5일까지 정자의 운동성과 수명을 보조생식술에 적합한 수준으로 유지시킬 수 있는 최적 배양 환경으로 제시할 수 있다. Objectives: To determinate the optimal culture condition to maintain lifespan in human sperm, we evaluated the effect of different temperature on sperm motility and viability up to 5 days in normal specimens. Methods: Ejaculated semen samples with normal semen parameters were gently washed in HEPES buffered Tyrod's-Albumin-Lactate-Pyruvate (HTALP) media. Each 5 ml of HTALP + 0.3% albumin with $1{\times}10^6$ sperm/ml was incubated for 5 days in $37^{\circ}C$, $22^{\circ}C$, and $4^{\circ}C$. The sperm motility and kinematics were analyzed using computer assisted sperm analysis (CASA), membrane integrity was assessed by hypoosmotic swelling test (HOST), and capacitation status was evaluated by chlorotetracycline (CTC) fluorescence pattern. Each parameter was measured on day 1, 3, and 5, respectively. Results: The motility, viability and live/uncapacitated pattern were demonstrated significantly in temperature- and time-dependent difference (p<0.05). While the sperm cultured for 1 day in each temperature was not significantly different, the sperm cell kept in $22^{\circ}C$ after 3 days were preserved sperm motility, viability, membrane integrity, and F pattern better than in other culture temperatures. Conclusions: HTALP can be used a basic medium for culture and longevity preservation, and sperm cell kept at $22^{\circ}C$ is beneficial for assisted reproductive techniques.
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秦漢시기 城市의 형성과정과 지리정보의 구축 : 서북지역 考古城址와 묘장의 분포양상을 중심으로
유영아(You, Young Ah) 한국외국어대학교 역사문화연구소 2021 역사문화연구 Vol.78 No.-
Under the goal of examining in what forms walled cities were built in northern regions, this study set out to conduct quantitative analysis by putting together the settlement relics of Han and adding tomb locations to the old archeological city sites mentioned as walled cities. That is, archeological city sites, tombs, and all kinds of relics that were treated separately were integrated to handle walled cities as settlements in a broader sense. In addition, the study would offer an overview of the formation processes of 郡and 縣in local areas for their application and review again the ways walled cities were formed based on the distribution patterns of settlements during Han. There is a need to consider tombs in research on the formation process of walled cities because they offer grounds to figure out changes to the concerned areas according to different periods. Since tombs are important elements to show the existence forms of settlements, the study will focus on the diversity of tomb styles in the cemeteries as well as tomb sizes. The distribution of different tomb styles will provide an indicator to show a society in northern regions, revealing that various tomb styles were created among different northern regions. The study examined the local characteristics of a society in northern regions by checking the locations of tombs in northern regions during Han and making their distribution map according to their attributes.
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戰國·秦漢시대 城市의 형성원인에 대한 연구: GIS를 이용한 考古城址의 분포와 입지분석을 중심으로
유영아(You, Young Ah) 한국외국어대학교 역사문화연구소 2019 역사문화연구 Vol.70 No.-
본 논문에서는 고고성지를 중심으로 戰國·秦漢시대의 성시의 형성원인을 고찰하기 위하여 유적정보에 대한 데이터베이스 정보를 작성하였다. 고고유적에 대한 데이터베이스 구축은 주변 환경과의 관계를 분석하기 위하여 우선되어야 할 과제이다. 그 정보를 기반으로 시기와 지역, 규모별로 분포도를 작성하였으며 규모와 기존의 군현성 등급간의 불일치 정도를 확인하였다. 이 문제를 해결하기 위해 기존의 군현내의 등급과 밀접하게 관련시켜 논의되어 왔던 성지의 규모를 성지가 축조되는 공간과의 관계 속에서 고찰해 볼 수 있는 방법들을 제시해 보았다. 황하중하류 지역에서 고고성지가 밀집되어 있는 하남성 일대를 중심으로 지리 정보를 작성하였다. 지리정보는 20미터 간격의 등고선을 기준으로 한 DEM(Digital Elevation Model)을 사용하였다. 그리고 일정한 水系의 범위를 기준으로 고고성지가 매우 밀집되어 있는 곳을 확인하였으며 전한시대 군경계를 중첩한 결과 고고성지들이 비교적 수계에 밀접하며 이것은 군의 경계지역과 관련이 높다는 것을 확인할 수 있었다. 이 때 고고성지의 규모는 북방지역의 성보 정도의 규모를 가지고 있다는 것도 확인할 수 있었다. In this study, the Database information on the cultural remains was prepared in order to find out why the city site was formed in the archaeological city states during Warring State period and Qin and Han dynasties. The construction of database on the ancient remains shall be first made in order to analyze the relation with the surrounding environments. Based on that information, the distribution maps for each period, region, and size were prepared and the degree of inconsistence between the size and the conventional grades in the local counties and prefectures was checked. To solve the problem, the method of considering the relation between the size of city site and the space where the city site was constructed, which has been discussed in close relation with the conventional grade in the existing counties and prefectures was suggested. The geological information has been prepared focusing on the area around Henan Prefecture where the ancient city site were concentrated in the area of lower and middle stream of Yellow river. As for the geological information, the contour with 20-meter distance was acquired using DEM(Digital Elevation Model)and thegradient(slope) was made using this information. Through this information, the place where the ancient city sites were closely concentrated was found to be around the rivers. When it was overlaid with the boundary of counties constructed during the Qin and Han dynasties, the ancient city sites were found to closely exist around the rivers, thus showing that they are closely related with the boundary of counties. At this time, the size of the ancient city sites had the size equivalent to that of a fortress lacated in the northern area.
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체외수정 및 미세조작에 의한 가축배의 생산과 효율적 이용에 관한 연구 6. 소 핵이식배의 체외배양 , 동결보존 및 성판정에 관한 연구
정영채,김창근,윤종택,나광빈,오성종,이종완,김흥률,김광식,박선애,유영아 ( Y . C . Chung,C . K . Kim,J . T . Yoon,G . B . Luo,S . J . Oh,J . W . Lee,H . R . Kim,K . S . Kim,S . A . Park,Y . A . You ) 한국축산학회 1997 한국축산학회지 Vol.39 No.3
This study was carried out to establish an effective method for the improvement of subsequent development after nuclear transfer(NT) in bovine embryo, for the extension of utility through freezing and sexing of NT embryos. The rates of cleaved and developed embryos upto morula-blastocyst stages after the NT in in vitro produced embryos were 60.6 and 3.7%, respectively. Whereas, in in vivo embryos, slightly higher developments were obtained(70.8 and 5.6%), respectively. No difference was found in embryonic development upto morulablastocyst stages among the different levels of EGF added to the serum containing medium. The rates of cleaved and developed embryos upto morula-blastocyst were higher when electrostimulation was performed either before fusion or after addition at 21h IVM. The survival rate of frozen NT embryos was higher in late morula than that of earlier embryos. PCR-based sexing of NT embryo demonstrated that higher proportion of embryos was male(1.9:1), instead of normal ratio(1:1).
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