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Pseubomonas aeruginosa의 Temperature Sensitive 突然變異體의 유도와 분리
유관희,정기흥,정순정,이형환 건국대학교 1983 論文集 Vol.16 No.1
Sixty-one temperature-sensitive mutants of Pseudomonas aeruginosa have been isolated from the mutagenized culture with N-methyl-N'-nitro-Nitrosoguanidine. The mutagen was treated at 36℃ and the ts-mutants were isolated at the non-permissive temperature of 42℃ by size of colony and growth. Twenty-five mutants were smaller in the size of colonies at 42℃ than those of 36℃, and thirty-sin mutants were not growed at 42℃.
Bacillus sphaericus ts - D1290 치사돌연변이주의 단백질 패턴
이형환,유관희,서정희 한국유전학회 1990 Genes & Genomics Vol.12 No.3
Bacillus sphaericu; ts-D1290 lethal mutant was comparatively characterized with the wild type strain 1593 by the measurements of the biosynthesis of total proteins on the temperature-sensitive cultures at permissive temperature of 30℃ and at nonpermissive-temperature of 42℃. The protein syntheses in the spores of the ts-D1290 and the wild type strain at 30℃ occurred in 6 to 8 hours after inoculation. When the spores of the mutant were cultured at 42℃, they germinated, but the protein synthesis almost did not occur, and the number of viable cells did not increase at 42℃. Vegitative cells after the germination of the wild type spores at 30℃, proteins of 140 and 150 kilodaltons produced within 1 or 2 hours in the cells; however, after a 2 h culture, the amounts of the 150 Kd protein increased. While the 140 kd protein did not produced in the mutant, only the 150 Kd protein produced in the cell, The 155 kd protein produced after a 4 h culture at 30℃.
Flavobacterium multivorum HL-1이 생산하는 Dextran 분해효소의 특성
서은숙,유관희,이형환 한국산업미생물학회 1995 한국미생물·생명공학회지 Vol.23 No.1
Flavobacterium multivorum HL-1이 생성하는 dextranase의 특성과 이 효소의 활성에 미치는 영향을 조사하였다. 덱스트란 분해효소의 활성은 Somogyi 방법에 의한 유리 환원당 측정과 3,5-dinitrosalicylic acid(DNS)에 의한 환원당 측정방법을 이용하였다. 상기 균주는 배지에 1.0%의 덱스트란이 첨가되었을 때에 생산이 최고치에 달했고, 이 배지에 ammonium nitrate와 beef extract를 첨가했을 때에 효소의 활성은 각각 570과 680 units/㎖로서 더욱 높았다. 효소의 활성은 Mg^(++) 첨가했을 때에 124%의 증가를 보였고, 반면에 Ca^(++), Co^(++), Hg^(++), Zn^(++)는 효소의 활성을 저해했다. 효소이 활성은 pH 4∼10에서 거의 비슷한 활성도를 보였으며, 최적 온도는 45∼55℃ 였다. Dextranase produced by Flavobacterium multivorum HL-1 isolated from soil were characterized. Optimum growth condition for the production of the enzyme by the strain appeared to be at the 1.0% concentration of dextran. When NH_4NO_3 and beef extract was added to the culture media, the enzyme activity increased upto 570 and 680 units per ㎖ respectively, and other nitrogen sources did not increase the activity. Urea, casamino acid, (NH_4)_2SO_4 and KNO_3 inhibited the activity. In the presence of Mg^(2+) in culture media, the enzyme activity increased 124%, but its activity was inhibited in the presences of Ca^(2+), Co^(2+), Hg^(2+) and Zn^(2+). The optimum temperature for the enzyme activity was 45∼55℃. In the ranges of pH 4 to 10, the activity of the enzyme appeared approximately similar.
Corynebacterium glutamicum ATCC 13058 의 Histidine 생합성 유전자 hisC 의 클로닝과 염기서열 분석
김수영,김경아,김현수,이형환,이승현,유관희,박갑주,이명석 한국유전학회 1997 Genes & Genomics Vol.19 No.4
The chromosomal DNA fragments of Corynebacterium glutamicum ATCC 13058 containing genes involved in histidine biosynthesis were cloned in pBluescript SK(+). To investigate the structural organization and the regulation of these genes, recombinant clones complementing E. coli hisA, hisB, hisC, hisI and hisF mutants were isolated from genomic library. A recombinant plasmid containing a 5.6-kb fragment of genomic DNA was able to complement E. coli hisA, hisB and hisF. But pCGH 13, carrying insert of 1.7-kb complemented only E. coli hisC. The genetic data indicate that these genes are clustered in a small region of the chromosome. Nucleotide sequence analysis of the 1.7-kb fragment of genomic DNA revealed one putative open reading frame which showed significant homology with the hisC gene of E. coli K-12 and Lacctococcus lactis.