RIA 와 EIA 에 의한 C 형 간염항체 측정법과 성적보고

민경선,조만익,원우재,서일택 대한임상검사과학회 1992 대한임상검사과학회지(KJCLS) Vol.24 No.1

종양 표지자 CA19-9의 Hook effect와 Quality control에 대한 개선안

김지나,김진희,원우재 대한핵의학기술학회 2004 핵의학 기술 Vol.9 No.1

종양 표지자 CA 19-9의 Hook effect와 Quality contr이에 대한 개선안

김지나,김진희,원우재 대한핵의학기술학회 2004 핵의학 기술 Vol.9 No.2

장비정도관리에 Calibrator[I-125] Set 적용

김지나,안재석,원우재,Kim, Ji-Na,An, Jae-seok,Won, Woo-Jae 대한핵의학기술학회 2015 핵의학 기술 Vol.19 No.2

Purpose Lately, in accordance with the increasing interest about Healthcare accreditation program and International laboratory accreditation scheme, requirements about the instrument quality management are gradually taking shape. In nuclear medicine In vitro laboratory, the most typical instruments are multi detector gamma counter and automatic dispensing system. Each laboratory continue with the quality control adequate for circumstances. The purpose of this study is to application and establish the new Calibrator[I-125]Set which is efficient at standardization of equipment quality management. Materials and Methods Deviation between detectors were measured with 12 solid samples of the Calibrator[I-125]Set. their activities differ from each other by less than 1%. Multi detector gamma counters are GAMMA-10;Shinjin medics. Inc, Goyansi, Korea(Gamma counter A), SR300;Stratec biomedical systems AG, Gewerbestr, Germany(Gamma counter B) and COBRA II; Packard Instrument Co. Inc, Meriden, USA(Gamma counter C). Evaluation of two automatic dispensing system used A, B liquid tracer of the Calibrator[I-125]Set. After dispensing and counting, calculated using the ratio of the measured value and proposed value. We used solution A for 20, 25ul and solution B for 50, 100ul. Method of data analysis and reference range was provided by kit documentation. Furthermore, we could calculate our counter efficiency indirectly. Results The CV(%) of measured values by Gamma counter A, B, C are 0.34, 0.70, 1.30. Calculated value are 1.05314, 2.10419, 4.08485. Provided reference range is less than 3. A dispensing system's calculated values are 0.986, 0.989, 1.023, 1.017 and B are 0.874, 0.725, 1.021, 0.904. Provided reference range is from 0.95 to 1.05. Also, counter's efficiency are 74.18, 72.79, 74.32% at counter A, B, C and efficiency of the one detector counter is 79.26%. Conclusion If using this Calibrator[I-125]Set after verifying whether quality assurance, is applicable to equipment quality management on behalf of the role of gold standard.

Thyroglobulin 검사 시 재검선별 및 결과보고절차마련

김지나,박광서,원우재,Kim, Ji-Na,Park, Kwang-Seo,Won, Woo-Jae 대한핵의학기술학회 2014 핵의학 기술 Vol.18 No.1

Purpose: Serum thyroglobulin (Tg) determination has been reported to be a sensitive indicator of persistent or recurrent differentiated thyroid cancer (DTC) after total thyroidetectomy. In patients free of metastasese and recurrences after a complete thyroidectomy and radioiodine removal for DTC, the Tg is usually <1 ng/mL or can no longer be detected even with TSH stimulation. Therefore, report in low Tg levels and selecting criteria for retest is very important. The purpose of this study was to establish selecting criteria for retest which is efficient at reliability improvement and Turn around time (TAT). Materials and Methods: Sera from 137 patients with DTC were divided into two groups as first(<1.0 ng/mL or >4.0 ng/mL) and second(1.0-4.0 ng/mL). In case of group(<1.0 ng/mL) is in patient free of metastases and recurrences, >4.0 ng/ml is low coefficient of variation (CV%) at internal quality control and good linearity at standard curve. Therefore first group began with Delta/Panic check (D/P) and second group surveyed with the latest results. In that the latest results were <1.0 ng/ml, we checked the thyroxin withdrawal. Finally selected specimen retested with raw specimen. Results: In first group, we was able to reduce the retest rate(30.8% to 7.7%). and In second group, 40% to 5%. The total retest rate was 7.3%. Conclusion: If using the selecting criteria for retest, is helpful to accuracy and quickness of the result report.

갑상선암에서의 혈중 Thyroglobulin 농도와 침생검 검체 Washout Solution의 Thyroglobulin 농도와의 상관관계

안재석,김지나,원우재,An, Jae-Seok,Kim, Ji-Na,Won, Woo-Jae 대한핵의학기술학회 2009 핵의학 기술 Vol.13 No.3

목적: 혈중 thyroglobulin 농도 측정(혈중 TG)은 갑상선암 수술 후 재발 및 전이여부를 판단하는데 중요한 지표이다. 최근엔 혈중 TG와 함께 침생검 검체의 washout solution을 이용한 TG 농도 측정(washout TG)이 추적 검사에 빈번히 사용되고 있다. 본 연구는 혈중 TG와 washout TG간에 어떠한 연관성이 있는지 알아보고자 한다. 대상 및 방법: 2007년 1월부터 2008년 2월까지 국립암센터에 내원한 갑상선암 환자 중 washout TG 와 혈중 TG를 함께 측정한 47명 중 혈중 Anti-Tg Ab 양성(${\geq}$100 U/mL)인 환자 6명을 제외한 41명을 대상으로 하였다. 혈중 TG와 washout TG와 세포검사결과와의 연관성도 조사하였다. 결과 및 결론: Washout TG는 혈중 TG보다 현저하게 높았으며 두 경우의 검사결과 간에 유의한 차이를 보였다(p=0.0394). washout TG와 세포검사결과와의 일치도는 87.8%, 혈중 TG와의 일치도는 56.1%였다. washout TG가 양성인 경우는 28명(세포검사결과 양성: 24, 양성의심: 4), 음성인 경우는 13명(세포검사결과 양성의심: 1, 음성: 12)이었다. serum TG가 양성인 경우는 26명(세포검사결과 양성: 17, 양성의심: 3, 음성: 6), 음성인 경우는 15명(세포검사결과 양성: 8, 양성의심: 1, 음성: 6)이었다. 갑상선암에서의 추적 검사로써 washout TG와 혈중 TG가 상호보완적으로 시행되어질 때 재발과 전이를 찾아내는데 유용하다. Purpose: The most widely accepted tool for follow up management of thyroid cancer patients is serum thyroglobulin (Tg) measurement, but its value is limited by the interference of anti-thyroglobulin antibodies (anti-Tg Ab). Recently thyroglobulin measurement in the wash out of fine-needle aspiration biopsy specimens (Tg-FNAB) is frequently used for differential diagnosis of recurrences/metastases. The aim of this study was the investigation of the diagnostic utility of Tg-FNAB compared with serum Tg. Materials and Methods: We enrolled 41 consecutive patients with thyroid cancer who were evaluated for Tg-FNAB between January 2007 and February 2008 retrospectively. We ruled out 6 patients who anti-Tg Ab positive (${\geq}$100 U/mL) in the RIA (BRAHMS anti-Tgn RIA 100Det; BRAHMS Aktiengesell schaft, Berlin, Germany). Serum Tg and Tg-FNAB were measured by immunoradiometric assay (BRAHMS Tg pluS RIA 100 Det; BRAHMS Aktienge sellschaft, Berlin, Germany). We evaluated for Tg-FNAB compared with serum Tg and corresponding cytological smear. To compare the values of the two the t-test was used. Results: Tg-FNAB values were significantly higher (median 1,060 ng/mL, range 0.2~434,000 ng/mL) than serum Tg (median 2.5 ng/mL, range 0.9~131 ng/mL) (p=0.0394). The rate of correspondence with Tg-FNAB between cytological result was 87.9% and 65.9% in the case of serum Tg. Tg-FNAB was positive in 28 (24 with positive and 4 with suspicious cytology). Of the remaining 13 patients with negative Tg-FNAB, 1 had suspicious and 12 had unsuspicious cytology. serum Tg was positive in 26 (17 with positive and 3 with suspicious and 6 with unsuspicious cytology), Of the remaining 15 patients with negative serum Tg, 8 was positive in cytological result and 1 had suspicious and 6 had unsuspicious cytology. Conclusions: Tg-FNAB measurement is more accurate with high sensitivity (87.9%) than serum Tg (65.9%). The Tg-FNAB was a useful predictor for detecting recurrences/metastases with serum Tg.

갑상선암에서의 혈중 Thyroglobulin 농도와 침생검 검체의 Washout solution의 Thyroglobulin농도와의 상관관계

안재석,김지나,원우재 대한핵의학기술학회 2008 핵의학 기술 Vol.12 No.4

Thyrogen 투여 후 측정된 Thyroglobulin 농도변화

김지나,안재석,원우재,Kim, Ji-Na,An, Jae-seok,Won, Woo-Jae 대한핵의학기술학회 2018 핵의학 기술 Vol.22 No.1

Purpose Assessment of Serum Thyroglobulin (sTg) value in total thyroidectomy patients having an ablation dose of radioactive iodine indicates remaining cancer or metastasis. Especially, sTg in patients on withdrawal thyroxine or thyrogen administration for radioiodine ablation is an important indicator to determine the direction of further treatment and prognosis. Current guidelines suggest measurement of sTg is performed at 72 hours after the last injection of thyrogen. and assumes that sTg reaches maximum serum levels at that time. The purpose of this study is to evaluate the variation of sTg measured after thyrogen administration. Materials and Methods We compared with sTg performed at 24hours(D0) and 72hours(D2) after the last injection of thyrogen. We reviewed D0 and D2 from 276 patients were divided them into three groups according to ablation dose of radioactive iodine, 5mCi(A group), 30~80mCi(B group) and 100~200mCi(C group). We used T-test for comparison between D0 and D2. sTg was measured in serum using immunoradiometric assay (Tg-plus RIA; BRAHMS, Berlin, Germany). Results There is no critical variation between D0 and D2 in A group(n=100)(P=0.32), The case of increase(D2>D0) is 45, no change(D2=D0) is 23, decrease(D2<D0) is 32. The biggest increase is the 3.0 ng/mL from 2.9 to 5.9 in A group. In B group(n=155, 30mCi:154, 80mCi:1), the difference is meaningful(P<0.05) and D2>D0 is 91, D2=D0 is 28, D2<D0 is 36. The biggest increase is 2.6 ng/mL from 3.5 to 6.1. In C group(n=21, 100mCi:19, 200mCi:2), D2>D0 is 19, D2=D0 is 2. The biggest increase is 143.6 ng/mL from 98.4 to 242. Conclusion There was a significant difference in the group over 30mCi. and the case of D2>D0 is 45%, 58.7%, 90.5% for each group. therefore, D2 increased as the dose of radioactive iodine increased. Furthermore, the most sTg values of D0 and D2 are variation under 2.0 ng/mL, so reproducibility as well as sensitivity of sTg will be important at values below 2ng/mL.

방사면역측정법에 의한 랩시노겐의 측정실험

신금철 ( C H Shin ),원우재 ( U J Won ),서일택 ( I T Seo ) 대한임상검사과학회 1991 대한임상검사과학회지(KJCLS) Vol.23 No.1

Serum pepsinogen assay by radioimmunoassay is necessary test to diagnostic of the digestive system disease . Assay result is 20-SOng/ml in normal serum, display more than 80ng/ml in patient``s serum. Recovery test is 96-107 percent and dilution test display almost a straight line. In the precision, coefficient of variation of within-assay is 6. 9% in the low level, 4. 8% in the middle level and 3. 5% in the high level. Coefficient of variation of between-assay display 11.7% in the low, 7.4% in the middle and 5.4% in the high level.

방사성동위원소 및 방사성 의약품 분주장치의 자체제작

홍성탁,박광서,김석기,원우재,전흥기,강세훈,최낙빈 대한핵의학기술학회 2009 핵의학 기술 Vol.13 No.4