시알릴락토즈의 대량생산 및 사업화

우진석 한국생물공학회 2010 한국생물공학회 학술대회 Vol.2010 No.10

TRPC3 cation channel plays an important role in proliferation and differentiation of skeletal muscle myoblasts

우진석,Chung-Hyun Cho,Do Han Kim,이은희 생화학분자생물학회 2010 Experimental and molecular medicine Vol.42 No.9

During membrane depolarization associated with skeletal excitation-contraction (EC) coupling, dihydropyridine receptor [DHPR, a L-type Ca2+ channel in the transverse (t)-tubule membrane] undergoes conformational changes that are transmitted to ryanodine receptor 1 [RyR1, an internal Ca2+-release channel in the sarcoplasmic reticulum (SR) membrane] causing Ca2+ release from the SR. Canonical-type transient receptor potential cation channel 3 (TRPC3), an extracellular Ca2+-entry channel in the t-tubule and plasma membrane, is required for full-gain of skeletal EC coupling. To examine additional role(s) for TRPC3 in skeletal muscle other than mediation of EC coupling,in the present study, we created a stable myoblast line with reduced TRPC3 expression and without α1SDHPR (MDG/TRPC3 KD myoblast) by knock-down of TRPC3in α1SDHPR-null muscular dysgenic (MDG) myoblasts using retrovirus-delivered small interference RNAs in order to eliminate any DHPR-associated EC coupling-related events. Unlike wild-type or α1SDHPR-null MDG myoblasts, MDG/TRPC3 KD myoblasts exhibited dramatic changes in cellular morphology (e.g., unusual expansion of both cell volume and the plasma membrane, and multi-nuclei) and failed to differentiate into myotubes possibly due to increased Ca2+ content in the SR. These results suggest that TRPC3 plays an important role in the maintenance of skeletal muscle myoblasts and myotubes.

Fe에 의해 활성화된 목질계 바이오차를 혼입한 모르타르의 전도성능

우진석,김애화,최원창,서수연,윤현도 한국구조물진단유지관리공학회 2024 한국구조물진단유지관리공학회 논문집 Vol.28 No.2

본 연구는 시멘트 복합체 기반 변형감지 센서 제조를 위한 전도성 충진재로 Fe 활성화된 목질계 바이오차를 사용하는 타당성을 조사하기 위해 진행되었다. Fe에 의해 활성화된 바이오차를 3% 혼입한 시멘트 복합체의 압축강도 및 전기적 특성을 평가하기 위해50×50×50mm³ 크기의 입방체 시험체 3개와 40×40×80mm³ 크기의 각기둥의 시멘트 복합체 기반 센서 3개를 각각 준비하였다. 시멘트 복합체기반 센서에는 전기저항 측정의 4탐침 방식이 사용되었다. Fe 활성화된 바이오차를 혼입한 시멘트 복합체 기반 센서의 경우 다양한 함수율 및반복압축하에서 임피던스와 같은 전기저항과 전도성을 조사하였다. 그 결과 시멘트 복합체 기반 센서의 직류 회로에서의 전기저항률이 시간이 지남에 따라 증가하였고, 저항률의 최대 부분 변화가 900초 동안 함수율이 증가함에 따라 감소하였다. 함수율 7.5% 구간에서 전도성 충진재로 Fe에 의해 활성화된 바이오차를 3% 혼입한 시멘트 복합체의 전도성이 가장 안정적이지만, 시멘트 복합체 기반 변형감지 센서의 압축변형과 비저항의 변화비(FCR)에서 반복압축응력이 증가함에 따라 변형감지능력에 있어서 다소 떨어지는 경향을 나타냈다. This study was conducted to examine the feasibility of using Fe-activated wood-derived biochar as a conductive filler for manufacturing cement-based strain sensor. To evaluate the compressive and electrical properties of cement composite with 3% Fe-activated biochar, three cubic specimens of size 50 x 50 x 50mm³ and three prismatic cement-based sensors of size 40 x 40 x 80mm³ were prepared respectively. The four-probe method of electrical resistance measurement was used for cement-based sensors. For cement-based sensors with FE-activated biochar, the conductive performance such as electrical resistance and impedance under different water content and repeated compression was investigated. Results showed that the fractional changes in the DC electrical resistivity of cement-based sensors increase with increasing time and the maximum fractional changes in the resistivity decrease with increasing the moisture contents during 900s. At moisture content of 7.5% range, the conductive performance of cement composite including 3% Fe-activated biochar as a conductive filler showed the most stable, while the strain detection ability tended to decrease somewhat as the repeated compressive stress increased between repeated compressive strain and fractional change in resistivity (FCR).

EIS와 SEM을 활용한 골재별 시멘트 경화체의 계면 특성 분석

우진석(Woo, Jin-Seok),성홍경(Sung, Hong-Kyeong),정보상(Jung, Bo-Sang),정수미(Jeong, Su-Mi),박선규(Park, Sun-Gyu) 대한건축학회 2023 대한건축학회 학술발표대회 논문집 Vol.43 No.2

Mitochondrial Transplantation Ameliorates the Development and Progression of Osteoarthritis

이아람,우진석,이선영,나현식,조권형,이연수,이정수,김선애,박승환,김석중,조미라 대한면역학회 2022 Immune Network Vol.22 No.2

Osteoarthritis (OA) is a common degenerative joint disease characterized by breakdown of joint cartilage. Mitochondrial dysfunction of the chondrocyte is a risk factor for OA progression. We examined the therapeutic potential of mitochondrial transplantation for OA. Mitochondria were injected into the knee joint of monosodium iodoacetate-induced OA rats. Chondrocytes from OA rats or patients with OA were cultured to examine mitochondrial function in cellular pathophysiology. Pain, cartilage destruction, and bone loss were improved in mitochondrial transplanted-OA rats. The transcript levels of IL-1β, TNF-α, matrix metallopeptidase 13, and MCP-1 in cartilage were markedly decreased by mitochondrial transplantation. Mitochondrial function, as indicated by membrane potential and oxygen consumption rate, in chondrocytes from OA rats was improved by mitochondrial transplantation. Likewise, the mitochondrial function of chondrocytes from OA patients was improved by coculture with mitochondria. Furthermore, inflammatory cell death was significantly decreased by coculture with mitochondria. Mitochondrial transplantation ameliorated OA progression, which is caused by mitochondrial dysfunction. These results suggest the therapeutic potential of mitochondrial transplantation for OA.

A focus on extracellular Ca2+ entry into skeletal muscle

조정현,우진석,Claudio F Perez,이은희 생화학분자생물학회 2017 Experimental and molecular medicine Vol.49 No.-

The main task of skeletal muscle is contraction and relaxation for body movement and posture maintenance. During contraction and relaxation, Ca2+ in the cytosol has a critical role in activating and deactivating a series of contractile proteins. In skeletal muscle, the cytosolic Ca2+ level is mainly determined by Ca2+ movements between the cytosol and the sarcoplasmic reticulum. The importance of Ca2+ entry from extracellular spaces to the cytosol has gained significant attention over the past decade. Store-operated Ca2+ entry with a low amplitude and relatively slow kinetics is a main extracellular Ca2+ entryway into skeletal muscle. Herein, recent studies on extracellular Ca2+ entry into skeletal muscle are reviewed along with descriptions of the proteins that are related to extracellular Ca2+ entry and their influences on skeletal muscle function and disease.

Streptomyces sp. GCA0001로 부터의 신규 항생물질 Cystocin의 구조분석, 생물활성 및 유도체제조

김자용,이희찬,우진석,송재경 호서대학교 반도체제조장비국산화연구센터 2001 반도체장비학술심포지움 Vol.2001 No.-

본 발명의 Puromycin 유도체인 Cystocin 화합물은 유기 합성에 의해 제조된 물질이 아니라 방선균계열의 신균주인 Streptomyces sp GCA0001로부터 추출된 신규 물질로서, 항박테리아, 항종양 및 항바이러스 활성 등의 생물학적 미생물 활성면에서 종래의 Puromycin 화합물에 비해 현저히 뛰어난 효과를 지니고 있고 Streptomyces sp GCA0001로부터 추출, 분리 및 정제 과정을 통해 제조된 자연의 선택의 과정을 거친 화합물이므로, Puromycin을 대체할 수 있는 획기적인 물질로 볼 수 있다. Cystocin, a derivative of Puromycin, is a new material derived from Streptomyces sp GCA0001, new strain of Actinomycetes spiecies.This compound has outstanding biological activities in anti-bacteria, anti-tumor and anti-virus than former Puromycin compounds.And it is chosen by natural selection processing through extraction, isolation and purification from, so it may replace old Puromycins in most applications.

Streptomyces sp GCA0001로부터 Cystocin의 생산 및 분리 정제

김동현,이희찬,우진석,송재경,류광경,김대희,강선엽 호서대학교 반도체제조장비국산화연구센터 2001 반도체장비학술심포지움 Vol.2001 No.-

Cystocin을 생산하는 Streptomyces sp GCA0001균주를 screenig하였으며, Streptomyces sp GCAO001를 배양하고 분리정제를 통하여 흰색 분말의 Cystocin을 얻을 수 있었다.Cystocin은 Streptomyces alboniger에서 생산되는 Puromycin과 매우 유사한 구조의 항생물질로 확인되었다.Cystocin은 Puromycin과 마찬가지로 단백질 합성시 폴리펩티드 사슬의 elongation을 조기 종결함으로써 생체내에서 항박테리아, 항종양 및 항바이러스 활성을 나타낸다. Cystocin is identified from the screening of Streptomyces sp GCAO001.It is isolated from the culture of Streptomyces sp GCAO001.A white coloured cystocin is structurally similar to puroinycin, an antibiotic produced by streptomyces alboniger.Like puromycin, cystocin possesses an antibacterial, antitumour and antiviral activity.It impairs the protein synthesis by inhibiting polypeptide chain elongation.

Vitamin D Attenuates Pain and Cartilage Destruction in OA Animals via Enhancing Autophagic Flux and Attenuating Inflammatory Cell Death

Jhun JooYeon,우진석,Kwon Ji Ye,Na Hyun Sik,Cho Keun-Hyung,김선애,Kim Seok Jung,문수진,Park Sung-Hwan,조미라 대한면역학회 2022 Immune Network Vol.22 No.4

Osteoarthritis (OA) is the most common form of arthritis associated with ageing. Vitamin D has diverse biological effect on bone and cartilage, and observational studies have suggested it potential benefit in OA progression and inflammation process. However, the effect of vitamin D on OA is still contradictory. Here, we investigated the therapeutic potential of vitamin D in OA. Six-week-old male Wistar rats were injected with monosodium iodoacetate (MIA) to induce OA. Pain severity, cartilage destruction, and inflammation were measured in MIA-induced OA rats. Autophagy activity and mitochondrial function were also measured. Vitamin-D (1,25(OH)2D3) and celecoxib were used to treat MIA-induced OA rats and OA chondrocytes. Oral supplementation of vitamin D resulted in significant attenuations in OA pain, inflammation, and cartilage destruction. Interestingly, the expressions of MMP-13, IL-1β, and MCP-1 in synovial tissues were remarkably attenuated by vitamin D treatment, suggesting its potential to attenuate synovitis in OA. Vitamin D treatment in OA chondrocytes resulted in autophagy induction in human OA chondrocytes and increased expression of TFEB, but not LC3B, caspase-1 and -3, in inflamed synovium. Vitamin D and celecoxib showed a synergistic effect on antinociceptive and chondroprotective properties in vivo. Vitamin D showed the chondroprotective and antinociceptive property in OA rats. Autophagy induction by vitamin D treatment may be a promising treatment strategy in OA patients especially presenting vitamin D deficiency. Autophagy promoting strategy may attenuate OA progression through protecting cells from damage and inflammatory cell death.

Biosynthesis of TDP-deoxysugar in vitro and in vivo

김대희,정영수,우진석,강선엽,이희찬,송재경 한국공업화학회 2004 한국공업화학회 연구논문 초록집 Vol.2004 No.1