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Molecular Cloning and Anti-fungal Effect of Endo-β-1,3-glucanase from Thermotoga maritima
우장빈,강해나,이수복 한국식품과학회 2014 Food Science and Biotechnology Vol.23 No.4
A gene encoding an endo-β-1,3-glucanasefrom Thermotoga maritima MSB8 (TmβG) was clonedand expressed in Escherichia coli. The purified enzymeproduced various β-1,3-glucooligosaccharides from solublelaminarin, and mainly β-1,3-glucooligosaccharides smallerthan laminaritetrose from insoluble curdlan. The optimumpH and temperature of the enzyme were 5.0 and 80ºC,respectively. TmβG inhibited the growth of Candidaalbicans, which indicates that the enzyme could potentiallybe used as an anti-fungal agent to control invasive infections.
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프로바이오틱스 Bifidobacterium breve BB077 안전성 평가
김병용,우장빈,한지윤,서은솔,서민영 한국식품위생안전성학회 2022 한국식품위생안전성학회지 Vol.37 No.5
Probiotics are live microorganisms that confer health benefits onto the host when administered at adequate doses. Most widely used probiotics, such as lactobacilli and bifidobacteria, are known to be elements of healthy gut microflora and hence are not considered a threat to the host. However, probiotics may pose a risk in certain populations with compromised immune systems or defects in gut barrier functions. Herein, we evaluated the safety of Bifidobacterium breve BB077, according to the safety evaluation guidelines for probiotics produced by the National Institute of Food and Drug Safety Evaluation (NIFDS). The results show that B. breve BB077 is both non-hemolytic and non-cytolytic. In contrast, B. breve BB077 exhibited higher streptomycin and tetracycline resistance than the suggested NIFDS standard cut-off values. Hence, a genetic analysis of the streptomycin and tetracycline resistance genes was performed to determine the origin of antimicrobial resistance. Streptomycin and tetracycline resistance was shown have arisen from chromosomal mutations and considered intrinsic to the taxonomic group. In conclusion, the B. breve BB077 strain might be safe for human consumption. 프로바이오틱스가 널리 사용됨에 따라 European Food Safety Authority (EFSA)와 식품의약품안전평가원이 제시하는 프로바이오틱스 안전성 평가 가이드라인에 준수하여 B. breve BB077의 안전성을 평가하였다. 본 실험에 사용한 B. breve BB077 균주는 용혈성이 발생하지 않았으며, 독소를거의 생성하지 않았음을 확인하였다. 항생제 내성 평가에서는 7종의 항생제에서는 EFSA 권고 cut-off value 보다 낮은 항생제 내성을 보였으며, 2종의 항생제인 streptomycin 와 tetracycline에서는 cut-off value 보다 높은 항생제 내성을 보였다. streptomycin, tetracycline에 대한 내성 결정인자는 B. breve 종의 공통 유전적 특징으로 외부로부터 전달받은 내성이 아닌 자연돌연변이에 의한 획득내성이기에 사용에 안전한 균주임을 확인하였다.
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프로바이오틱스 혼합물의 in vitro에서의 안전성 및 탄수화물 소화 저해 효능 평가
서은솔,우장빈,서민영,우정민 한국식품저장유통학회 2023 한국식품저장유통학회지 Vol.30 No.3
This study aimed to assess the in vitro safety of a probiotics mixture (Lactobacillus acidophilus PBS066, Lactiplantibacillus plantarum PBS067, and Limosilactobacillus reuteri PBS072), along with its inhibitory effect on carbohydrate digestion. All three strains met the antibiotic resistance profile of the European Food Safety Authority (EFSA) guidelines. None of the strains exhibited hemolytic activity or cytotoxicity against Caco-2 cells. Strains PBS067 and PBS072 inhibited α-amylase activity, whereas all three strains suppressed α-glucosidase activity, indicating that the mixture might limit carbohydrate digestion in the gastrointestinal tract. These findings support the safety of this probiotics mixture and its potential to modulate carbohydrate metabolism in the gut.
ScienceON
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