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      • KCI등재후보

        질소이용 및 효소활성의 기능성 복합미생물을 이용한 축분퇴비화

        왕옌칭,신재형,박상민,김수기 (사)한국축산환경학회 2023 한국축산시설환경학회지 Vol.25 No.2

        This research focused on the use of composite microbial preparations, derived from the complex strains (Alcaligenes aquatilis, Rhodococcus jostii, Alcaligenes faecalis, Lysinibacillus varians, Bacillus megaterium, Bacillus velezensis, Bacillus subtilis, Glutamicibacter arilaitensis, Cupriavidus nectar, Bacillus licheniformis, Pediococcus acidilactici and Bacillus sp. SinR4), in compost production in cold regions. These strains, isolated from livestock manure, compost, and soil, were examined for their heat stress resistance and antagonism. About 30 days after putting microorganisms in the compost (T), It showed that the treatment group had a higher average temperature than the control, with the peaks being 60.1°C and 58.9°C, respectively. Moreover, the treatment group had less production of gases like ammonia, amine, and hydrogen sulfide, and a shorter deodorization time than the control group. The results suggest that the use of such composite microorganisms can enhance compost production by promoting temperature increase, reducing odorous gas production, and inhibiting thermal stress.

      • KCI등재후보

        Supplemental effects of different production methods of pine needle additives on growth performance, intestinal environment, meat quality and serum of broiler chickens

        Chang Yi-Qiang,Moon Seung-Kyu,왕옌칭,Xie Liu-Ming,Cho Hang-sul,김수기 아세아·태평양축산학회 2024 Animal Bioscience Vol.37 No.7

        Objective: Pine needles are rich in many nutrients and exhibit antibacterial and antioxidant biological activities; however, the effects of different production methods of pine needle additives on the growth performance and intestinal flora of broiler chickens are not known.Methods: Normal diets were supplemented with pine needle fermentation juice (PNF), pine needle soaking juice (PNS), or pine needle powder (PNP), and the associated effects on growth performance, relative organ weights, intestinal development, intestinal histological morphology, intestinal flora, meat quality, and serum indicators in broiler chickens were observed.Results: The results showed that PNF, PNS, and PNP all significantly improved feed utilisation and promoted the growth and development of broilers. All three additives also significantly improved the structure of the intestinal flora, specifically increasing the diversity of bacteria; increasing the abundance of beneficial bacteria, such as <i>Faecalibacterium, Rikenella</i>, and <i>Blautia</i>; and decreasing the abundance of harmful bacteria, such as <i>Staphylococcus</i>. The antioxidant properties of pine needles were also found to intensify lipid metabolic reactions in the blood, thus leading to lower triglycerides and total cholesterol. Meanwhile, high doses of PNF reduced jejunum and ileum weights and also increased meat yellowness. Lastly, none of PNF, PNS, or PNP had an effect on relative organ weights or intestinal histological morphology.Conclusion: The addition of pine needles to the diet of broiler chickens can effectively promote their growth performance as well as improve their intestinal flora and serum status without side effects; in particular, the dose of 0.2% of either PNF and PNS is expected to have the capacity to replace growth-promoting antibiotics as diet additives. Objective: Pine needles are rich in many nutrients and exhibit antibacterial and antioxidant biological activities; however, the effects of different production methods of pine needle additives on the growth performance and intestinal flora of broiler chickens are not known. Methods: Normal diets were supplemented with pine needle fermentation juice (PNF), pine needle soaking juice (PNS), or pine needle powder (PNP), and the associated effects on growth performance, relative organ weights, intestinal development, intestinal histological morphology, intestinal flora, meat quality, and serum indicators in broiler chickens were observed. Results: The results showed that PNF, PNS, and PNP all significantly improved feed utilisation and promoted the growth and development of broilers. All three additives also significantly improved the structure of the intestinal flora, specifically increasing the diversity of bacteria; increasing the abundance of beneficial bacteria, such as Faecalibacterium, Rikenella, and Blautia; and decreasing the abundance of harmful bacteria, such as Staphylococcus. The antioxidant properties of pine needles were also found to intensify lipid metabolic reactions in the blood, thus leading to lower triglycerides and total cholesterol. Meanwhile, high doses of PNF reduced jejunum and ileum weights and also increased meat yellowness. Lastly, none of PNF, PNS, or PNP had an effect on relative organ weights or intestinal histological morphology. Conclusion: The addition of pine needles to the diet of broiler chickens can effectively promote their growth performance as well as improve their intestinal flora and serum status without side effects; in particular, the dose of 0.2% of either PNF and PNS is expected to have the capacity to replace growth-promoting antibiotics as diet additives.

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