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맥각균의 배양에 관한 연구 ( 3 ) 발효조에서의 Lysergic Acid 알칼로이드의 생성
김병각,옥선,최응칠 ( Byong Kak Kim,Sun Auck,Eung Chil Choi ) 생화학분자생물학회 1973 BMB Reports Vol.6 No.3
Ergot fungus was culitvated in stirred fermentors in various modified conditions to investigate their influence on the production of lysergic acid alkaloids. Claviceps paspali Stevens and Hall Isolate No. 31 was cultured in mannitol-succinate medium of Schwarting, et al., and such modifications as additions of Tween 80, oxygen gas, dimethylsulfoxide, arsenate and the utilization of sorbitol-fumaric acid medium and propeller-type impellers were respectively examined. Quantitative determination of the total alkaloids was carried out using Michelon-Kelleher method. The results showed that additions of oxygen gas, dimethylsulfoxide, arsenate, and the utilization of sorbitol-fumaric acid medium increased the production of the alkaloids. However, the addition of Tween 80 caused extensive foaming and resulted in decrease of the alkaloid accumulation.
맥각균의 배양에 관한 연구(III) - 발효조에서의 Lysergic Acid 알칼로이드의 생성
김병각,옥선,최응칠,Kim, Byong-Kak,Auck, Sun,Choi, Eung-Chil 생화학분자생물학회 1973 한국생화학회지 Vol.6 No.3
맥각균을 발효조에서 배양할때 라이서직산 알칼로이드의 생성에 미치는 배양조건 및 첨가물질의 영향을 검토하기 위하여 Claviceps paspali Stevens and Hall 31호 균주를 Schwarting 배양액에서 배양하였으며 Tween 80, 분자상의 산소, 디메칠설폭사이도, 비산 나트륨을 각각 첨가했을 경우 및 솔비톨-푸마르산 배양액과 프로펠라형 교반기를 사용했을 경우의 영향을 실험하였다. 알칼로이드 총량의 정량은 Michelon-Kelleher 법을 이용하였다. 실험결과에 의하면 산소, 디메칠성폭사이드, 비산염의 각각 첨가 및 솔비톨-프마르산 배양액의 사용이 알칼로이드 생성을 증가하였다. 그러나 Tween 80의 첨가는 과잉의 포말을 발생시켜 배양액의 손실을 초래하였으며 결국 알칼로이드의 축적이 감소되었다. Ergot fungus was culitvated in stirred fermentors in various modified conditions to investigate their influence on the production of lysergic acid alkaloids. Claviceps paspali Stevens and Hall Isolate No.31 was cultured in mannitol-succinate medium of Schwarting, et al., and such modifications as additions of Tween 80, oxygen gas, dimethylsulfoxide, arsenate and the utilization of sorbitol-fumaric acid medium and propeller-type impellers were respectively examined. Quantitative determination of the total alkaloids was carried out using Michelon-Kelleher method. The results showed that additions of oxygen gas, dimethylsulfoxide, arsenate, and the utilization of sorbitol-fumaric acid medium increased the production of the alkaloids. However, the addition of Tween 80 caused extensive foaming and resulted in decrease of the alkaloid accumulation.
한국산 고등균류의 성분연구 (Ⅲ) - 한국산 맥각의 Alkaloid 성분
김병각(Byong Kak Kim),황성현(Sung Hyun Hwang),옥선(Sun Auck),응권(Eung Kwon Lee) 한국생약학회 1973 생약학회지 Vol.4 No.1
Korean ergot sclerotia which were collected from Agropyron semicostatum NEES, Miscanthus sinensis ANDERS. var. purpurascens REND, M. sacchariflorus BENTH. et HOOK, M, sinensis ANDERS., Eulalia speciosa O. KUNTZ, Themeda triandra FORSK. var. japonica MAKINO, and Phalaris arundinacea L. were investigated for their alkaloid constituents. Quantitative determinations of alkaloids were carried out using a modified procedure of van URK and MICHELON-KELLEHER methods. The results showed that the two ergots from Agropyron semicostatum and Phalaris arundinacea contained total alkaloids up to 0.137 per cent. The alkaloids of these two ergots are mostly water-insoluble alkaloids. The ergots parasitizing Reed Canary grass was found to contain both water-soluble and water-insoluble alkaloids. The TLC and PPC tests for the ergot alkaloids from Agropyron semicostatum and Phalaris arundinacea revealed that they contain at least two alkaloids, respectively, and that the ergot from Themeda triandra FORSK. var. japonica has one. The lipid contents of the seven ergots ranged from 13 to 29 per cent.
김병각,옥선 생화학분자생물학회 1978 BMB Reports Vol.6 No.3
Optimal conditions for the production of lysergic acid alkaloid by Claviceps paspali isolate No. 31 in stirred fermentors were investigated. Effects of surfactants, glycine, arsenate, dimethylsulfoxide, oxygen, and different impellers were examined.