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오진표,정규환 한국원예학회 2012 Horticulture, Environment, and Biotechnology Vol.53 No.5
This study was carried out to deduce an appropriate analysis method to evaluate the homogeneity of the agricultural characteristics of genetically modified (GM) crops for environmental risk assessment. Comparative analyses were made between GM hot pepper lines and their control lines cultivated in the same year, between different GM peppers carrying the same gene but in different chromosomal loci, between the same GM peppers cultivated in different years and two different GM peppers carrying different genes from three GM peppers (B20, H15, and T20) and their control lines (P915, P2377, and THK). The aim was to determine the most appropriate comparison combination to establish the evaluating protocol and environmental risk assessment. Data of 57 different characteristics of pepper plants,green fruits and red fruits was collected during the optimal vegetation period. Of the 57 different characteristics, 24characteristics were excluded as there was no difference and the remaining 33 characteristics were analyzed by t-test. There were statistical differences in five characteristics between the B20 and P915 lines, five characteristics between the H15 and P2377 lines and two characteristics between the T20 and THK lines cultivated either in 2008 or in 2011. On the other hand, there were significant differences in 11 characteristics between the B20 and H15 lines cultivated in 2008 and 14 characteristics between the H15 and T20 harvested in 2011. Comparative analysis of 33agricultural characteristics between the H15 line cultivated in 2008 and 2011 showed the most significant differences. Based on this result, the comparison between the same GM lines cultivated in different years was judged meaningless when the homogeneity of agricultural characteristics of GM crops were evaluated. In conclusion, the comparative analysis between GM peppers and their control lines cultivated in the same year is the most appropriate while the homogeneity of agricultural characteristics is examined to assess environmental risk of GM peppers.
신육종기술을 통해 육성된 계통의 세대 진전을 위한 소포자 배양 조건 탐색
오진표(Jinpyo Oh),안정민(Jeong Min An),김범기(Beom-Gi Kim),임찬주(Chan Ju Lim) 한국원예학회 2021 한국원예학회 학술발표요지 Vol.2021 No.10
CRISPR-Cas9을 이용한 유전자 교정 기술은 세균류가 외부로부터 침입한 병원체의 유전정보를 저장하여 후에 재침입시 이를 절단하는 후천적 면역 시스템과 손상된 DNA를 교정하는 시스템을 이용한 기술로 3세대 유전자 교정 기술에 속한다. 기본적인 개념음 가이드 RNA로 목표 서열을 설정한 뒤 DNA의 이중 나선을 자르면 이를 복구하는 과정에서 발생하는 돌연변이로 목표 서열이 가지고 있는 coding을 무력화시키는 기술이다. 기술의 특성상 기존의 coding region을 삽입하는 GMO와 달리 기능을 넣기보다는 해당 부위가 가지고 있는 기능을 결손시키는데 특화된 기술이다. 생물의 유전체는 모계와 부계로부터 각각 한 세트씩 전달받아 한쌍을 이루고 있으며, 한 쪽 염기서열상에 결손이 발생하더라도 다른 쪽의 완전한 염기설열이 이를 보완할 수 있어 표현형에는 영향을 미치지 못하기 때문에 해당 부위 기능의 결손을 유도하여 만들어진 형질은 열성유전의 양상을 띄게 된다. 열성 유전에 의한 형질은 유전체가 동형접합인 상태에서만 형질이 발현되기 때문에 이를 육종 소재로 이용하기 위해서는 빠르고 안정적인 열성 동형접합체가 필요하다. 전통적인 육종을 통해서는 순계집단 육성을 위해서는 5~7년 이상의 시간이 필요하나 소포자 배양등 반수체 배양을 이용하면 1~2년 내로 순계집단을 육성할 수 있다. 이번 연구에서는 신육종실용화사업단 연구과제에 사용되고 있는 십자화과 유전자원들의 원활한 세대 진전 및 순계집단 육성을 위한 반수체배양 조건을 탐색하고자 하였다. 배추, 양배추, 브로콜리의 소포자배양 효율을 높이기 위해서 washing 배지와 배양배지의 종류를 B5 배지와 NLN 배지를 사용하였으며, 첨가한 NAA와 BA의 함량을 달리하여 생장조절 물질의 비율에 따른 배 발생율과 재분화율을 확인하였다. 또한 배지내 activated charcoal의 유무에 따른 효율도 함께 평가하였다. 양배추의 경우 세 계통을 대상으로 8종의 배지의 효율을 평가하여 BN640 계통의 배 발생율과 식물체 유기율이 높았으며, 브로콜리의 경우 washing 배지를 B5로, 배양 배지는 1/2 NLN 배지를 사용할 경우 배양 효율이 높았다. 배추의 경우 washing 배지와 배양 배지 모두 NLN 배지를 사용한 배양에서 가장 우수한 효율을 기록하였다. 첨가된 물질의 함럄의 경우 sucrose 15%에 저농도 NAA와 BA를 동량 첨가한 처리구에서 배 발생율과 재분화율이 높았다. activated charcol을 petri dish 당 10, 25, 50mg씩 첨가한 결과 25mg을 첨가한 실험구가 다른 실험구에 비해 높은 배발생율을 보였으나 정상적인 배 발생율은 50mg을 첨가한 실험구에서 높았다.
중ㆍ북부지역에서 재배된 GM 배추와 Non-GM 배추간의 식물체 특성 및 영양 성분 비교 분석
조동욱(Dong Wook Cho),오진표(Jin Pyo Oh),박권우(Kuen Woo Park),이동진(Dong Jin Lee),정규환(Kyu Hwan Chung) 한국원예학회 2010 원예과학기술지 Vol.28 No.5
This study was carried out to investigate plant characteristics and nutritional components of the genetically modified (GM) Chinese cabbage and its control line grown in the central and northern parts of Korea in order to establish the evaluating protocol and standard assessment. The GM and non-GM Chinese cabbage was planted with normal and concentrated density at two locations in spring and fall of 2008 and 2009. From the statistic analysis on plant characteristics and nutritional components, there were not many significant differences between GM and non-GM Chinese cabbage. Only few differences in the plant characteristics were found between the dense and normal planting. In the dense planting, there was no significant difference between GM and non-GM Chinese cabbages except for three out of 18 plant traits, such as leaf shape, hairiness and midrib length. On the other hand, nine plant traits including leaf length, leaf width, leaf color, leaf shape, fresh weigh of ground part, number of leaf, midrib length, midrib width and root diameter were slightly different between GM and non-GM Chinese cabbage in the normal planting. In case of leaf length, midrib length, midrib width and fresh weigh of ground part, there were significantly differences not only between two lines, but also between two locations. From nutritional component analysis, only five fatty acids were identified in the Chinese cabbage: palmitic acid, oleic acid, stearic acid, linoleic acid and linolenic acid. Except linoleic acid, four fatty acids in one gram of dried sample from GM line were little higher than those from non-GM line. However, there were no significant differences in total contents of fatty acids not only between GM and non-GM Chinese cabbage line, but also between northern and central cultivating areas in the normal and dense planting. According to the composition of inorganic elements identified in the samples from both lines, there were six macro-elements, such as N, P, Ca, K, Mg and Na, and four micro-elements, Cu, Fe, Mn and Zn. Based on the result from PCA analysis, specific clusters were not found between GM Chinese cabbage and the control line, but found between two regions.
과경 유관속 조직의 차이에 따른 ‘자옥’과 ‘흑보석’ 포도의 수확기 과실 연화
정명희(MyungHee Jung),오진표(Jin Pyo Oh),김준혁(JunHyeok Kim),박요섭(YoSup Park),권용희(YongHee Kwon),박희승(Hee-Seung Park) 한국원예학회 2016 원예과학기술지 Vol.34 No.5
The objective of this study was to determine the differences in fruit growth, fruit quality, and particularly the pedicel vascular bundles of ‘Shigyoku’ and ‘Heukboseok’ grapes, which appeared to be different in softening at harvest. ‘Shigyoku’ grape matured faster (by about 20 days) than ‘Heukboseok’ grape with slight fruit enlargement after veraison. However, fruit of ‘Heukboseok’ grapes showed remarkable enlargement in both the primary and secondary fruit enlargement periods. Hypodermal cell layers were not different after veraison in ‘Shigyoku’ grape, but degradation of the hypodermis cell wall continued in ‘Heukboseok’ grape, resulting in a gradual decline in firmness. The numbers of hypodermal cell layers in ‘Shigyoku’ and ‘Heukboseok’ grapes were 14.2 and 9.0, respectively. The average content of soluble solids in ‘Shigyoku’ grape (19.5°Brix) was significantly (p ≤ 0.01) higher than that of ‘Heukboseok’ grape (17.0°Brix). Xylem of the pedicel did not differ between the two varieties. However, average phloem area after veraison of ‘Shigyoku’ grape (19044.8 ㎛²) was about 1.8 fold greater than that of ‘Heukboseok’ grape (10509.4 ㎛²), based on the number of cells constituting the phloem. The cell number and area of the phloem might affect the accumulation of sugars, the main constituents of the cell wall, thus maintaining the firmness of grapes until late maturity. Therefore, the increased softening of ‘Heukboseok’ grapes at harvest might due to their phloem structure.