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- 저자 : 오수경 ( Woo Ik Hwang (검색결과 2 건)
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황우익,이성동,오수경,Hwang, Woo-Ik,Lee, Sung-Dong,Oh, Soo-Kyung 생화학분자생물학회 1982 한국생화학회지 Vol.15 No.3
우리나라 한방에서 처방되고 있는 10종 생약제를 물로 추출하여 물액기스를 암세포의 일종인 L1210, P388 및 sarcoma 180 세포 등에 대해 in vitro와 in vivo에서 screening test하여 항암 활성 및 수명연장 효과를 측정하였다. In vitro에서의 항암 활성은 각 생약제 모두(대황 제외) L1210 보다 P388 세포에서 더 높게 나타났으며 동일 세포에 대한 각 생약제의 활성은 대황과 대극이 가장 높았고 감수와 삼능, 파두의 순으로 나타났다. 또한 각 추출물을 농도별로 첨가한 후 배양 시간 별로 항암 활성을 측정한 결과 일반적으로 일정농도 이상에서 9~12시간 배양 후 세포의 감소율이 가장 높았다가 24시간 후에 는 그 수준을 유지하거나 증가되는 경향을 보였다. 이러한 세포수의 감소 원인을 규명하기 위해 추출물 첨가 후 배양시의 세포 size 분포도 변화를 농도별, 시간별로 관찰하였다. 이 결과 추출물 첨가 직후에는 size 분포가 대조군과 일치하다가 6시간 배양 후 diameter가 적은 쪽으로 이동하였다. In vivo test에서 sarcoma 180 세포를 접종시킨 swiss mice에 각 추출물을 경구적 또는 복강내 주사로 투여한 결과 경구적 투여시의 수명연장 효과는 볼 수 없었다. 복강내 주사시는 초기(50% 사망시까지)에서는 감수, 나복자, 삼능, 도안 등에서 수명연장 효과가 인정되었으나 말기(100% 사망시까지)에는 별 효과를 나타내지 못하였다. Various Korean medicinal herbs have been used for the treatment of cancer patient in the orient. This study was devised to observe the potential antitumor activity of 10 kinds of Korean medicinal herbs against L 1210 and P 388 murine tumor cells in vitro, and against Sarcoma 180 cells in vivo. Ten kinds of herbs used in this experiment are as follows; Pa-doo, Dae-geuk, Kam-soo, Won-hwa, La-bok-ja, Sam-neung, A-chool, Dae-hwang, Hoi-hyang and Do-in. The growth rate, doseresponse and size distribution of L 1210and P 388 cells cultured in the medium with or without each herb water extract were determined, and also the effects of incubation time in same medium determined. The antitumor effects of each extract were estimated by the measurement of the increased survival time of the Swiss mice inoculated with Sarcoma 180 cells in vivo test. The experimental results obtained are summarized as follows; 1. in vitro test; a) Antitumor activity of each water extract of medicinal herb was much more active in the P 388 cells than in the L1210 cells(except Dae-hwang extract). b) In the culture medium of P 388 cell, the activity (1 unit) of each extract, Pa-doo, Dae-geuk, Kam-soo, Won-hwa, La-bok-ja, Sam-neung, A-chool, Dae-hwang and Hoi-hyang was applicable to 0.14, 0.11, 0.13, 0.18, 0.62, 0.13, 0.35, 0.15 and 0.45 mg, respectively. c) In the culture medium of L 1210 cells, the activity (1 unit) of each extract was applicable to 0.22, 0.13, 0.15, 0.24, 0.93, 0.14, 0.91, 0.11 and 1.38 mg, respectively. d) The numbers of the P 388 cells were significantly decreased by incubation with each herb extract for 9 to 12 hours. e) The size distribution of P 388 cells incubated with each herb extract for 6 to 9 hours were shifed to direction of the small size region. 2. in vivo test; The survival time of Swiss mice inoculated with Sarcoma 180 cells was slightly extended at early stage (until the 50% death), compared with control group, by peritoneal injection of the extract of Kam-soo, La-bok-ja, and Sam-neung although the effects were not appeared at last stage (100% death). In the case of the oral administration of the extracts, the effects were not appeared.
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황우익,이성동,오수경 ( Woo Ik Hwang,Sung Dong Lee,Soo Kyung Oh ) 생화학분자생물학회 1982 BMB Reports Vol.15 No.3
Various Korean medicinal herbs have been used for the treatment of cancer patient in the orient. This study was devised to observe the potential antitumor activity of 10 kinds of Korean medicinal herbs against L 1210 and P 388 murine tumor cells in roitro, and against Sarcoma 180 cells in vivo. Ten kinds of herbs used in this experiment are as follows; Pa-doo, Dae-geuk, Kam-soo, Won-hwa, La-bok-ja, Sam-neung, A-chool, Dae-hwang, Hoi-hyang and Do-in. The growth rate, doseiesponse and size distribution of L 1210and P 388 cells cultured in the medium with or without each herb water extract were determined, and also the effects of incubation time in same medium determined. The antitumor effects of each extract were estimated by the measurement of the increased survival time of the Swiss mice inoculated with Sarcoma 180 cells in vivo test. The experimental results obtained are summarized as follows; 1. in vitro test; a) Antitumor activity of each water extract of medicinal herb was much more active in the P 388 cells than in the L1210 cells(except Dae-hwang extract). b) In the culture medium of P 388 cell, the activity (1 unit) of each extract, Pa-doo, Dae-geuk, Kam-soo, Won-hwa, La-bok-ja, Sam-neung, A-chool, Dae-hwang and Hoi-hyang was applicable to 0.14, 0.11, 0.13, 0.18, 0.62, 0.13, 0.35, 0.15 and 0.45 ㎎, respectively. c) In the culture medium of L 1210 cells, the activity (1 unit) of each extract was applicable to 0.22, 0.13, 0.15, 0.24, 0.93, 0.14, 0.91, 0.11 and 1.38 ㎎, respectively. d) The numbers of the P 388 cells were significantly decreased by incubation with each herb extract for 9 to 12 hours. e) The size distribution of P 388 cells incubated with each herb extract for 6 to 9 hours were shifed to direction of the small size region. 2. in vivo test; The survival time of Swiss mice inoculated with Sarcoma 180 cells was slightly extended at early stage (until the 50 % death), compared with control group, by peritoneal injection of the extract of Kam-soo, La-bok-ja, and Sam-neung although the effects were not appeared at last stage (100 % death). In the case of the oral administration of the extracts, the effects were not appeared.
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