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Determination of Microbial Diversity in Gouda Cheese via Pyrosequencing Analysis
오상남,김영훈 한국낙농식품응용생물학회 2018 한국유가공기술과학회지 Vol.36 No.2
The present study aimed to investigate the microbial diversity in Gouda cheese within the four months of ripening, via next-generation sequencing (NGS). Lactococcus (96.03%), and Leuconostoc (3.83%), used as starter cultures, constituted the majority of bacteria upon 454 pyrosequencing based on 16S rDNA sequences. However, no drastic differences were observed among other populations between the center and the surface portions of Gouda cheese during ripening. Although the proportion of subdominant species was <1%, slight differences in bacterial populations were observed in both the center and the surface portions. Taken together, our results suggest that environmental and processing variables of cheese manufacturing including pasteurization, starter, ripening conditions are important factors influencing the bacterial diversity in cheese and they can be used to alter nutrient profiles and metabolism and the flavor during ripening.
Comparison of Total RNA Isolation Methods for Analysis of Immune-Related microRNAs in Market Milks
오상남,박미리,손석준,김영훈 한국축산식품학회 2015 한국축산식품학회지 Vol.35 No.4
Bovine milk provides essential nutrients, including immunologically important molecules, as the primary source of nutrition to newborns. Recent studies showed that RNAs from bovine milk contain immune-related microRNAs (miRNA) that regulate various immune systems. To evaluate the biological and immunological activity of miRNAs from milk products, isolation methods need to be established. Six methods for extracting total RNAs from bovine colostrums were adopted to evaluate the isolating efficiency and expression of miRNAs. Total RNA from milk was presented in formulation of small RNAs, rather than ribosomal RNAs. Column-combined phenol isolating methods showed high recovery of total RNAs, especially the commercial columns for biofluid samples, which demonstrated outstanding efficiency for recovering miRNAs. We also evaluated the quantity of five immune-related miRNAs (miR-93, miR-106a, miR-155, miR-181a, miR-451) in milk processed by temperature treatments including low temperature for long time (LTLT, 63℃ for 30 min)-, high temperature for short time (HTST, 75℃ for 15 s)-, and ultra heat treatment (UHT, 120-130℃ for 0.5-4 s). All targeted miRNAs had significantly reduced levels in processed milks compared to colostrum and raw mature milk. Interestingly, the amount of immune-related miRNAs from HTST milk was more resistant than those of LTLT and UHT milks. Our present study examined defined methods of RNA isolation and quantification of immune-specific miRNAs from small volumes of milk for use in further analysis.
오상남,김영훈,문용일 한국유산균·프로바이오틱스학회 2016 Current Topic in Lactic Acid Bacteria and Probioti Vol.4 No.1
Social needs are increasing due to the increase of degenerative brain diseases because of the aging society. The most common type of degenerative brain diseases is Alzheimer's disease and Parkinson's disease, which makes most of cases. New scientific challenges indicate that gut microbiota are directly associated with the progress of degenerative brain diseases through triggering metabolic diseases, modulating immune response, and changing diversity of microbial community. A high-valuable strategy is proposed for the management of these disorders and as an adjuvant for psychiatric treatment of degenerative brain diseases and other related metabolic diseases through modulation of the gut microbiota including probiotics.
오상남,권대호,이은일 대한독성 유전단백체 학회 2011 Molecular & cellular toxicology Vol.7 No.3
DNA damage in normal cells by reactive oxygen species can trigger cellular senescence and tumorigenesis. Therefore, many strategies have been developed to inhibit DNA damage and identify molecules that can prevent various oxidative stress-related diseases, including cancer. To investigate the effect of pressure, preconditioning with two types of atmospheric pressure (hyperbaric mild hyperoxia compressed with air (EP) and hyperbaric normoxia with nitrogen gas (HN)) was applied to normal human lung fibroblasts (WI-38) for 1 or 3 days, and then cells were treated with hydrogen peroxide to induce oxidative damage. Here, we have elucidated two types of pressure that can protect WI-38 cells from oxidative stress-induced DNA damage. To profile the expression of genes that contribute to the cytoprotective effects of the pressures,microarray analyses were performed. Extracellular matrix-, cell adhesion-, and cell cycle-related genes were highly involved in the cytoprotective effects of the pressures. Among the significantly expressed genes, fibronectin was the most inducible in WI-38cells under the cytoprotective conditions against oxidative damage. Additionally, cellular fibronectin, and not the secreted isoform, acted as a cytoprotective marker at these pressures. Together, these findings suggest that elevated static pressures exhibit cytoprotective activity against oxidative stress-induced DNA damage by inducing cellular fibronectin.
오상남(Sangnam Oh),김영훈(Younghoon Kim),문용일(Yong Il Moon) 한국유산균프로바이오틱스학회 2016 Current Topic in Lactic Acid Bacteria and Probioti Vol.4 No.2
Social needs are increasing due to the increase of degenerative brain diseases because of the aging society. The most common type of degenerative brain diseases is Alzheimer s disease and Parkinson s disease, which makes most of cases. New scientific challenges indicate that gut microbiota are directly associated with the progress of degenerative brain diseases through triggering metabolic diseases, modulating immune response, and changing diversity of microbial community. A high-valuable strategy is proposed for the management of these disorders and as an adjuvant for psychiatric treatment of degenerative brain diseases and other related metabolic diseases through modulation of the gut microbiota including probiotics.
김명곤,오상남,이지혜,임오섭,류연미,오은하,이주현,이은일,설동근 생화학분자생물학회 2004 Experimental and molecular medicine Vol.36 No.5
In this study, we investigated the efects of PAHs and dioxin on mRNA and plasma protein expresion using genomic and proteomic ana-lysis for automobile emision inspectors and waste incineration workers. About 54 workers from automobile emission inspection ofices, 31 workers from waste incinerating company and 84 unexposed healthy subjects were enroled in this study. Urine and air samples were colected says were caried out to evaluate any DNA damage in mononuclear and polynuclear cels. A significant diference in Olive tail moments in mononuclear cels was observed between ex-posed and control subjects (P< 0.0001). To examine the diferences of the gene expresion profile in automobile emission inspectors and waste incineration workers, radioactive comple-mentary DNA microarays were used to evaluate The gene expression profiles showed that 11 genes were up-regulated and 4 genes were down-regulated in waste incinerating workers as compared with controls. Plasma proteins were analyzed by 2-dimentional electrophoresis with pH 3-10 NL IPG Dry strip. The protein expres-sion profiles showed that 8 proteins were up- regulated and 1 protein, haptoglobin, was down- regulated in automobile emision inspectors and waste incineration workers. Serum paraoxonase/ arylesterase was found only in the plasma of genes and proteins involved in oxidative stres were up-regulated in both automobile emission inspectors and waste incineration workers. Se-veral proteins, such as transthyrethin, sarcol-ectin and haptoglobin, that were highly up- or down-regulated, could serve as biological moni-toring markers for future study.