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감국천연향료의 추출 방법 및 추출 성분의 유해성에 관한 연구
오다원,이병철,이인수,김운중 한국인그리에학회 2019 한국인그리에학회지 Vol.1 No.2
Natural fragrances were extracted from Chrysanthemum indicum L. belonging to asteraceae, native to Korea, variously used as medicinal plants. Extraction methods were extracted by solvent extraction and supercritical fluid extraction. The solvent of the solvent extraction method was ethanol and ether, and the supercritical extraction method was performed using only supercritical carbon dioxide and supercritical carbon dioxide with cosolvent ethanol. The extraction was carried out in total four conditions. The fragrance extracted according to the conditions was qualitatively analyzed by GC-MS to derive fragrance components. In addition, since it is used directly on the skin when used as a perfume raw material, ICP-MS is used to determine the heavy metal content of four types of nickel (Ni), arsenic (As), cadmium (Cd), and lead (Pb) confirmed. In order to determine whether the Chrysanthemum indicum L. fragrance is applicable not only to perfumes but also to various functional cosmetics, functional characteristics such as antimicrobial and antioxidant effects of the extracts were studied. The fragrance extracted by solvent extraction yielded 0.63% when using ethanol and 1.89% when using ether based on 70g of raw materials. The fragrance extracted by supercritical extraction yielded 2.41% using supercritical fluid only and 5.30% using supercritical fluid with cosolvent. As a result of GC-MS qualitative analysis of the extract, the components with the smell of Herbal and Floral were commonly detected as 2-pinene, eucalyptol and camphor. According to the sensory analysis of fragrances, we received the highest scores in the 'light' and 'soft' scores. Antimicrobial experiments confirmed that all four conditions had antimicrobial activity against staphylococcus aureus As a result of this study, the highest yield condition was found in the perfume extraction process, and the Chrysanthemum indicum L. perfume is expected to be useful for functional cosmetics applied to the skin.