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저온 프린팅 공정을 통한 콜라겐/실크-피브로인/세포외기질이 포함된 조직재생용 세포담체 개발
허준태(JunTae Huh),이형진(Hyeongjin Lee),여명구(MyungGu Yeo),김용복(YongBok Kim),양지훈(Gi-Hoon Yang),구영원(YoungWon Koo),여미지(Miji Yeo),임정형(JungHyung Lim),김근형(GeunHyung Kim) 대한기계학회 2016 대한기계학회 춘추학술대회 Vol.2016 No.12
In tissue engineering, biomaterials must be biocompatible, biodegradable, and mechanically stable to be used for biomedical scaffold. Among various biomaterials, natural polymers are widely used for great biocompatibility. However, due to their low mechanical properties, it has been a challenge to build desired 2D/3D structure using natural polymers. To overcome this problem, in this study, we used low temperature 3D printing to create a scaffold with collagen/silk-fibroin(SF)/extracellular matrix(ECM). Then, fibroblast cells were grown on the fabricated scaffold. Various in vitro cellular activities (cell-viability, MTT assay) for pure collagen, pure SF, collagen/SF, and collagen/SF/ECM scaffolds were compared. The collagen/SF/ECM scaffold showed 30% higher cell metabolic activities than pure collagen scaffold.