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조혈전구세포의 시험관 증식 및 분화에 대한 HoxB4와 STAT3의 상호조절 연구
홍성현 ( Sung Hyun Hong ),양승집 ( Seung Jip Yang ),김진아 ( Jin A Kim ),오일환 ( Il Hoan Oh ) 한국조직공학·재생의학회 2007 조직공학과 재생의학 Vol.4 No.2
Self-renewal of hematopoietic stem cells(HSC) is a major mechanism for regeneration of blood tissues from HSCs. Previous studies have identified HoxB4 and STAT3 as an important molecular mechanisms regulating HSC self-renewal, but potential interaction of the two remains unknown. To address the issue, transgenic mice for constitutively activated STAT3(STAT3-C) was examined for HoxB4 expression, but no difference in the HoxB4 expression level was seen compared to the control mice. On the contrary, when HoxB4 was co-transduced with dominant negative(dnSTAT3), the enhancing effect of HoxB4 on the proliferation of 5-FU stimulated bone marrow cells were significantly decreased as compared to the HoxB4 alone group. Similarly, the effect of HoxB4 on the maintenance of undifferentiated state as determined by lineage marker was significantly deteriorated by co-expression of dnSTAT3. Taken together, these results suggest that STAT3 activity could be a down-stream of HoxB4, being required for HoxB4-mediated enhancing effects on in vitro cultured hematopoietic precursor cells.