경쟁적인 RT-PCR에 의한 흰쥐 뇌 e-fos 유전자의 발현 양상 분석

양보기,채영규 한양대학교 이학기술연구소 1999 이학기술연구지 Vol.1 No.-

경쟁적인 RT-PCR에 의한 흰쥐 뇌 c-fos 유전자의 발현 양상 분석

양보기,채영규 한양대학교 이학기술연구소 1999 이학기술연구지 Vol.1 No.-

자극에 따른 흰쥐 뇌 c-fos 유전자 발현 변화 양을 정량적으로 검출하기 위하여, 저자들은 선조체, 대뇌피질, 해마, 및 소뇌에서 조직을 취하였다. 흰쥐에서 분리된 각각의 뇌 조직에 RT-PCR 반을을 수행하였다. 뇌 조직에 따른 전체 RNA당 각각 발현된 c-fos Mrna 양은 소뇌가 143, 대뇌피질이 24, 해마가 38, 그리고 선조체가 44 atto mole/㎍이었다. In order to detect the quantitative amount of change of c-fos gene expression after stimuli in the rat's brain, the author obtained brain specimens from the striatum, cerebral cortex, hippocampus, and cerebellum. Each brain was removed from the rat, then RT-PCR was perfromed. Each amount of c-fos expressed mRNA per total RNA was cerebellum 143, cerebral cortex 24, hippocampus 28, and striatum 44 amole/㎍, respectively.

Regulatory Eosinophils in Inflammation and Metabolic Disorders

양보기,서주영,장명호 대한면역학회 2017 Immune Network Vol.17 No.1

Eosinophils are potent effector cells implicated in allergic responses and helminth infections. Responding to stimuli, they release their granule-derived cytotoxic proteins and are involved in inflammatory processes. However, under homeostatic conditions, eosinophils are abundantly present in the intestine and are constantly in contact with the gut microbiota and maintain the balance of immune responses without inflammation. This situation indicates that intestinal eosinophils have an anti-inflammatory function unlike allergic eosinophils. In support of this notion, some papers have shown that eosinophils have different phenotypes depending on the site of residence and are a heterogeneous cell population. Recently, it was reported that eosinophils in the small intestine and adipose tissue, respectively, contribute to homeostasis of intestinal immune responses and metabolism. Accordingly, in this review, we summarize new functions of eosinophils demonstrated in recent studies and discuss their homeostatic functions.

Cytosine Arabinoside 유도된 PC12 세포의 사망 경로

양보기,양병환,채영규,Yang, Bo-Gee,Yang, Byung-Hwan,Chai, Young-Gyu 대한생물정신의학회 1998 생물정신의학 Vol.5 No.2

Cytosine arabinoside(AraC) inhibits DNA synthesis and ${\beta}$-DNA polymerase, an enzyme involved in DNA repair. This, a potent antimitotic agent, is clinically used as an anticancer drug with side effect of severe neurotoxicity. Earlier reports suggested that inhibition of neuronal survival by AraC in sympathetic neuron may be due to the inhibition of a 2'-deoxycytidine-dependent process that is independent of DNA synthesis or repair and AraC induced a signal that is triggers a cascade of new mRNA and protein synthesis, leading to apoptotic cell death in cultured cerebellar granule cells. The present study would suggest whether caspase family(ICE/CED-3-like protease) involved in AraC-induced apoptosis pathway of PC12 cells. It was observed that treatment of PC12 cells with AraC led to decrease of viability by MTT assay and morphology changes, which did not suggest that AraC induced apoptosis in PC12 cells. The mRNA of caspase-1/caspase-3 were expressed in PC12 cells constitutively, and AraC did not activate caspase family. These results suggest that caspase-1/caspase-3 may not be required for AraC-induced cell death pathway in PC12 cells.

Alterations in Gut Microbiota and Immunity by Dietary Fat

양보기,이명식,허규연 연세대학교의과대학 2017 Yonsei medical journal Vol.58 No.6

Gut microbiota play critical physiological roles in energy extraction from the intestine and in the control of systemic immunity, as well as local intestinal immunity. Disturbance of gut microbiota leads to the development of several diseases, such as colitis, inflammatorybowel diseases, metabolic disorders, cancer, etc. From a metabolic point of view, the gut is a large metabolic organ and one of the first to come into contact with dietary fats. Interestingly, excessive dietary fat has been incriminated as a primary culpritof metabolic syndrome and obesity. After intake of high-fat diet or Western diet, extensive changes in gut microbiota have been observed, which may be an underlying cause of alterations in whole body metabolism and nutrient homeostasis. Here, we summarizerecent data on changes in the gut microbiota and immunity associated with dietary fat, as well as their relationships with the pathogenesis of metabolic syndrome. These findings may provide insight into the understanding of the complex pathophysiologyrelated to the development of metabolic diseases and offer an opportunity to develop novel candidates for therapeutic agents.

광-펜톤산화를 이용한 마이크로시스틴 제거

양보람 ( Boram Yang ),정성목 ( Sung-mok Jung ),남혜림 ( Hye-lim Nam ),남아름 ( Aram Nam ),박정안 ( Jeong-ann Park ),최재우 ( Jae-woo Choi ),박희등 ( Hee-deung Park ),이상협 ( Sang-hyup Lee ) 한국물환경학회 2017 한국물환경학회·대한상하수도학회 공동 춘계학술발표회 Vol.2017 No.-

펜톤산화를 이용한 마이크로시스틴 제거

양보람 ( Boram Yang ),정성목 ( Sung-mok Jung ),남혜림 ( Hye-lim Nam ),박정안 ( Jeong-ann Park ),박찬혁 ( Chanhyuk Park ),이상협 ( Sang-hyup Lee ) 한국물환경학회(구 한국수질보전학회) 2016 한국물환경학회·대한상하수도학회 공동 춘계학술발표회 Vol.2016 No.-

실내공간에서 패브릭의 확산효과를 활용한 LED 조명 디자인의 특성 및 경향

양보람 ( Yang Boram ),정강화 ( Chung Kangwha ) 한국디자인트렌드학회 2011 한국디자인포럼 Vol.31 No.-

실내공간에서 빛은 단지 밝기를 제공한다는 의미를 넘어 하나의 소재로 인식되고 있다. 실내공간에서는 빛을 확산이 중요하기 때문에 조명기구에 있어 확산성과 빛의 효과를 고려한 투과면 소재의 선택이 필요하다. LED가 실내공간으로 적용이 확대됨에 따라 LED의 특성에 최적화된 조명기구의 개발이 요구되고 있다. 특히 LED는 소형화된 점광원, 글레어 방지를 이유로 빛의 확산 메커니즘에 대한 연구가 필요하다. 따라서 본 논문에서는 LED 조명 확산소재로써 패브릭의 가능성을 제시하고 패브릭의 확산효과를 활용한 LED 조명 디자인의 특성 및 경향을 연구하기 위해 사례를 선정하여 분석하였다. 분석의 기준은 형태적 특성과 표현 특성으로 분류했다. 사례들을 형태적 특성으로 분석한 결과 패브릭을 활용한 LED 조명이 독립적인 조명기구형태에서 공간에 일체화된 형태로 확장되는 경향이 나타났다. 또한 패브릭의 표현 특성인 드레이프성, 신축성, 촉감에 따라 다른 확산면이 형성되어 빛 확산효과가 나타남을 확인할 수 있었다. 본 연구의 결과는 LED 조명의 확산소재로써 패브릭이 디자인적 측면에서 형태적 자유도와 다양한 빛 확산효과의 장점이 있음을 보여준다. 패브릭 소재의 활용을 통해 스마트 라이팅 테크놀로지를 이질감 없이 자연스럽게 실내 환경에 적용시킬 수 있을 것으로 보인다. The light in interior space is being recognized as a material itself rather than just a thing that provides the brightness. Because of importance of the diffusion of light in interior, the choice of material considering the effects of diffusion and transmission of light is needed in lighting fixtures. In particular, there has to be an urgent research in regard to a mechanism of the diffusion of light on account of minimized light point resource, straightness of the light, and the protection of glare. Accordingly in this study, selected cases were analyzed to present a possibility of the usage of fabric as diffusing material and study characteristics and tendency of LED lighting design utilizing diffusion of fabric. Basis of analysis is classified with morphological characteristic and express characteristics. As a result, the analysis according to morphological characteristics, there was a tendency that the LED lighting utilizing fabric extends from a form of independent lighting fixture to a form unified in a space appeared. Also various diffusion surface formed and diffusing effect of light appeared to express characteristics of fabric such as drape, stretch, and tactile impression. This study shows that the fabric, a diffusing material of LED lighting has an advantage of morphological freedom and various lighting diffusion effects as in design aspect. It is expected to have smart writing technology applied to an interior environment in a natural way without a sense of difference through a utilization of fabric material.

구획증후군 진단을 위한 구획압 측정 핸드헬드 장치

양보미(B. M. Yang),문영진(Y. J. Moon) Korean Society for Precision Engineering 2022 한국정밀공학회 학술발표대회 논문집 Vol.2022 No.5

Cyclosporine 신경독성의 뇌 자기공명영상 소견

양보성 대한영상의학회 1998 대한영상의학회지 Vol.39 No.6

Purpose : To analyze the MR findings of cyclosporine-induced neurotoxicity in patients receiving high dose ofcyclosporine and to suggest the possible pathogenetic mechanism. Materials and Methods : The cases of sevenpatients (2 males, 5 females ; 18-36 years old) who suffered seizures after receiving high-dose cyclosporine forbone marrow transplantation due to diseases such as aplastic anemia or leukemia were retrospectively reviewed. Weevaluated the location and pattern of abnormal signal intensity seen on T2 weighted images, the presence ofcontrast enhancement, and the changes seen on follow-up MR performed at intervals of 12-30 days after initial MRin five of seven patients. We analyzed levels of blood cyclosporine and magnesium, and investigated the presenceof hypertension at the site of the seizure. Results : Locations of the lesions were bilateral(n=5),unilateral(n=2), parietal(n=6), occipital(n=6), temporal(n=4), and in the frontal lobe(n=3). Frontal lesionsshowed high signal intensities in the borderline ischemic zone of the frontal lobe between the territory of theanterior and middle cerebral arteries. In six of the seven patients, cortical and subcortical areas includingsubcortical U-fibers were seen on T2-weighted images to be involved in the parietooccipital lobes. Only one of theseven showed high signal intensity in the left basal ganglia. All lesions showed high signal intensity onT2-weighted images, and iso to low signal intensity on T1-weighted. In five of seven patients there was nodefinite enhancement, but in the other two, enhancement was slight. In four of seven patients seizures occurredwithin high therapeutic ranges (250 - 450 ng/ml), while others suffered such attacks at levels below thetherapeutic range. After cyclospirine was administered at a reduced dosage or stopped, follow-up MR images showedthe complete or near-total disappearance of the abnormal findings previously described. Only two patients hadhypertension, and the others normotension. Five of the seven had hypomagnesemia(1.3 -1.74 mg/dl; N : 1.9 -3.1mg/dl). Conclusion : Most patients with cyclosporine neurotoxicity showed high signal intensity in the corticaland subcortical areas of the parietooccipital lobes, including subcortical U-fiber, as seen on T2 weighted images,and no abnormal enhancement after Gd-DTPA injection. These MR findings should be helpful for the diagnosis ofcyclosporine neurotoxicity.