황금 추출물이 생쥐에 유발된 접촉피부염의 피부 증상에 미치는 영향

양버들(Beodul Yang),김수라(Sura Kim),최찬헌(Chan Hun Choi),정현우(Hyun Woo Jeoung),김형우(Hyungwoo Kim) 한의병리학회 2017 동의생리병리학회지 Vol.31 No.1

향심 주정추출물의 난소적출 랫드에서 항골다공증 효과

이현주,노종현,양버들,우경완,송용진,이에나,이융재,황태연,김선라,조현우,정용현,이무진 한국생약학회 2020 생약학회지 Vol.51 No.4

Lentinus edodes (LE) is the first medicinal macrofungus to enter the realm of modern biotechnology. Lentinus edodes is well-known to have anti-tumor, anti-inflammatory, and immunomodulatory effects. In this study, osteoporosis was induced by ovariectomy in female rats, and the prevention and treatment efficacy of the climacteric disease the postmenopausal type I pattern was examined using the experimental substance. Female rats were either sham-operated (sham; n = 5) or surgically ovariectomized (OVX; five animals per group) and then administered to OVX control, raloxifene hydrochloride 1 mg/kg/day, or LE (20 and 200 mg/kg/day) for 12 weeks. LE treatment suppressed the ovariectomy-induced reduction of bone mineral density in the entire tibia as well as its metaphysis with a decrease of serum osteocalcin and interleukin-6 levels. In addition, LE is suggested to elevate the serum levels of progesterone hormones and prevent bone loss in ovariectomized rats. In conclusion, LE 200 mg/kg/day may have inhibitory effects on osteoporosis in OVX rats.

Inhibitory Effects of Methanol Extract from Nardostachys chinensis on 27-hydroxycholesterol-induced Differentiation of Monocytic Cells

손용해,김형우,양버들,김보영,박영철,김관회 한국생약학회 2017 Natural Product Sciences Vol.23 No.4

27-Hydroxycholesterol (27OHChol) has been reported to induce differentiation of monocytic cells into a mature dendritic cell phenotype. We examined the effect of methanol extract of Nardostachys chinensis (Nard) on 27OHChol-induced differentiation using THP-1, a human monocytic cell line. Treatment of monocytic cells with methanol extract of Nard resulted in decreased transcription and surface expression of CD80, CD83, and CD88 elevated by 27OHChol in a dose-dependent manner. Surface levels of MHC class I and II molecules elevated by 27OHChol were also reduced to basal levels by treatment with the Nard extract. Decreased endocytosis activity caused by 27OHChol was recovered by treatment with the Nard extract. CD197 expression and cell attachment were attenuated by the Nard extract. In addition, levels of transcription and surface expression of CD molecules involved in atherosclerosis, such as CD105, CD137, and CD166 upregulated by 27OHChol were significantly decreased by treatment with methanol extract of Nard. These results indicate that methanol extract of Nard down-regulates 27OHChol-induced differentiation of monocytic cells into a mature dendritic cell phenotype and expression of CD molecules associated with atherosclerosis. The current study suggests that biological activity of oxygenated cholesterol derivatives can be inhibited by herbal medication.

민들레 뿌리 물 추출물의 류마티스 관절염 동물 모델에 대한 개선 효과

노종현,이현주,장지훈,양버들,김아현,우경완,황태연,서재완,조현우,정호경 한국약용작물학회 2019 한국약용작물학회지 Vol.27 No.1

Background: Taraxacum platycarpum has been used in traditional medicine in Korea to treat intoxication and edema and as a diuretic. According to previous reports, it has anti-cancer, anti-gastritis, and anti-inflammation effects. However, the improvement effect of T. platycarpum on rheumatoid arthritis has not been investigated. The anti-oxidative and anti-inflammation effects of the aerial parts of T. platycarpum are different from those of its subterranean parts. Thus, we evaluated the effect of the water extracts of Taraxaci radix (WTR) on type II collagen-induced rheumatoid arthritis (CIA) in animal models. Methods and Results: Rheumatoid arthritis was induced by type II collagen. WTR (100 ㎎/㎏ and 500 ㎎/㎏) was administered to the animal models. Methotrexate was used as the positive control. The levels of interleukin-6, TNF-alpha, and type II collagen IgG in the animals were measured by using enzyme-linked immunosorbent assay. Treatment with 500 ㎎/㎏ WTR decreased the serum levels of interleukin-6, TNF-alpha, and collagen IgG in the CIA models. Moreover, treatment with WTR diminished the arthritisinduced swelling of the hind legs and monocyte infiltration in the bloodvessels of the animal models. Conclusions: These results indicate that WTR has the potential to improve rheumatoid arthritis by reducing the levels of inflammatory cytokines such as interleukin-6 and TNF-alpha. However, further experiments are required to elucidate the influence of WTR on signal transduction in vitro and in vivo.

忍冬 열수 추출물의 항산화 효과 및 HCl-Ethanol로 유도된위염 동물 모델에서의 위 점막 손상 보호 효과

심미옥,이현주,장지훈,정호경,양버들,우경완,황태연,김선영,노종현,조현우 대한본초학회 2019 大韓本草學會誌 Vol.34 No.6

Objective : Gastritis is a major complication of gastrointestinal disease. Lonicera japonica is used in folk medicine to treat different diseases such as exopathogenic wind-heat, epidemic febrile diseases, sores, carbuncles and some infectious diseases. Therefore, this study examined the effects of Lonicera japonica water extract (LJE) on HCl/ethanol- induced acute gastric ulceration and anti-oxidants properties. Methods : LC-ESI-IT-TOF MS was employed for rapid identification of major compound from LJE. The antioxidant activities were evaluated through total polyphenol and flavonoid contents and radical scavenging assays and superoxide dismutase (SOD)-like activity. SD rats were randomly divided into five different groups including the normal group, ulcer group, positive group (20 ㎏/㎎ of omeprazole, ip), and experimental groups (100 ㎏/㎎ and 500 ㎏/㎎ of LJE, ip). Results : 4,5-Dicaffeoyl quinic acid, loganic acid, secologanic acid, sweroside, loganin, vogeloside were identified based on the detection of the molecular ion with those of literature data. The LJE was possessed free radical scavenging activities such as DPPH (IC50=189.7 ㎍/㎖), ABTS (IC50=164.5 ㎍/㎖), and SOD-like activity (IC50=405.02 ㎍/㎖). Macroscopic and histological analyses showed LJE treated group were significantly reduced to an extent that it allowed leukocytes penetration of the gastric walls compared with the ulcer group. In addition, an ulcer inhibition rate and prostaglandin E2 levels were increased in rats treated with LJE. Conclusion : The present study has demonstrated the antioxidantive and gastroprotective effect of LJE, these findings suggested that LJE has the potential for use in treatment of gastric disorders.

익모초 물 추출물의 위염 예방효과 및 소핵시험을 이용한 유전독성 평가

노종현,이현주,장지훈,양버들,우경완,김아현,서재완,김선영,조현우,정호경 한국자원식물학회 2019 한국자원식물학회지 Vol.32 No.4

Gastritis is an inflammatory disease involving the stomach and is caused by several factors, including stress, non-steroidal anti-inflammatory drugs such as aspirin, liquor, and Helicobacter pylori. In Korea, Leonurus japonicus Houttuyn (LJW) has been used as traditional medicine for vaginal bleeding, hematuria, and bruise. Previous studies have reported that LJW exhibited hepatoprotective, cardioprotective, and anti-hyperlipidemic effect. However, the effect of the water extract of LJW on gastritis was not elucidated. Thus, we evaluated the anti-gastric effect and genotoxicity of LJW. LJW effectively prevented the degeneration of surface mucous cells and glandular epithelial cells and vascular congestion induced by HCl/EtOH. Micronucleus assay indicated that the rate of micronucleated polychromatic erythrocytes/polychromatic erythrocytes was not significantly different compared that of the control. Further experiments are required to determine the role of LJW in the gastric injury process such as cyclooxygenase signaling pathway and the secretion of mucus in the stomach. 본 연구는 에탄올과 염산으로 유도된 위염모델에 대해 익모초의 물 추출물이 위염예방효과를 나타내는지 알아보기 위해진행하였고, 안전성 평가를 위해 유전독성평가인 소핵시험을수행하였다. 익모초 물 추출물은 음성대조군 대비 위에서 분비되는 PGE2의 농도를 증가시켰을 뿐만 아니라 염산과 에탄올 투여에 의한 점막 표피세포 및 선상피세포의 손상과 울혈을 충분히 예방하는 것을 확인하였다. 또한 익모초 물 추출물에 대한 소핵시험을 실시한 결과, 익모초 물 추출물은 소핵을 유발하지 않는 것으로 나타났다. 결과를 종합하였을 때 익모초 물 추출물은자체적으로 위염 예방효과를 나타냈지만 정확히 어떤 기전을통해 예방하는지 추가적인 실험이 필요하다고 사료되며, 익모초에 포함된 여러 단일 성분을 이용해 위염 예방평가를 실시하여, 위염에 대한 익모초의 효능과 효율을 높이는 연구가 필요하다고 생각된다.

동물모델을 이용한 짚신나물 물 추출물의 위염 예방효과 및 유전독성 평가

노종현,장지훈,이현주,양버들,우경완,김아현,서재완,황태연,조현우,정호경 한국약용작물학회 2019 한국약용작물학회지 Vol.27 No.2

Background: Agrimonia pilosa Ledeb has been used as a traditional medicine for the treatment of hematuresis and uterine bleeding in Korea. It has been reported to have anti-obesity, anti-diabetes and anti-inflammaotry effect by regulating the inflammatory signaling pathway. However, the preventive effect of Agrimonia pilosa Ledeb on gastritis has not been elucidated. Thus, in the present study, we evaluated the effects of the water extract of Agrimonia pilosa Ledeb (APW) using HCl/EtOH-induced gastritis rat models. Method and Results: Gastritis was induced in rats by HCl/EtOH administration. The rats in each group were orally administered with two doses of APW (100 and 500㎎/㎏). Omeprazole was used as a positive control drug. An enzyme-linked immunosorbent assay (ELISA) was used to measure the prostaglandin E2 (PGE2) levels in stomach. The treatment with 500㎎/㎏ APW reduced the gastric ulcer area. The APW treatment prevented a decreased in PGE2 concentration induced by HCl/EtOH in rats. In the micronucleus test, the ratio of micronucleated polychromatic erythrocytes to polychromatic erythrocytes showed no significant change in the APW-treated group compared with the control group. Conclusions: These results indicate that APW could be used to prevent the gastritis caused by the HCl/EtOH-induced damage to stomach lining. In addition, the APW treatment showed no significant change in results of the micronucleus test. However, further experiments are required to determine how APW influenced the secretion of mucus and gastric acid using the chromosome aberration test and bacterial reverse mutation assay.

독성시험관리기준 적용 갈근탕의 안전성 평가

노종현(Jong Hyun Nho),장지훈(Ji Hun Jang),이무진(Mu Jin Lee),양버들(Beodul Yang),우경완(Kyeong Wan Woo),이현주(Hyun Joo Lee),김아현(A Hyeon Kim),심미옥(Mi Ok Sim),조현우(Hyun Woo Cho),정호경(Ho Kyung Jung) 한국약용작물학회 2019 한국약용작물학회지 Vol.27 No.3