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안순혁(Soon Hyuk Ahn),최창운(Chang Woon Choi),임상무(Sang Moo Lim),오옥두(Ok Doo Awh),최태현(Tae Hyun Choi) 대한핵의학회 2002 핵의학 분자영상 Vol.36 No.2
목적: 유전자 치료에서, 종양세포에 herpes simplex virus thymidine kinase 유전자를 발현시키고 이에 민감한 prodrug을 전신투여하여 발현된 종양세포에 특이적으로 집적되는 방법은 매우 효육적인 바업이다. 대표적인 prodrug인, IVDU, IVFRU는 herpes simplex virus type 1의 비침습적 영상화 방법에 응용되는 방사옥소 표지가능 화합물이다. 재료 및 방법: Trans-1-trimethylsilyl-2-tri-n-butylstannylethylene과 uridine 변형체상의 iodo-uracil을 Pd촉매하에서 반응시켜 표지전구체인 5-trimethylvinyl-deoxy-uridine과 5-trimethylsilylvinyl-deoxy-fluororibofuranosyl uracil을 합성하였다. 이것을 column chromatography의 방법으로 분리 정제하였다. 합성한 각 전구체를 ICl 담체를 이용하여 높은 수율로 표지하였고, HPLC를 사용하여 분리하였다. Radiohalogen exchange 방법은 비방사능을 낮게 할수록 표지에 효율적으로 알려져 있다. 이 방법으로 ICl 방법을 사용하여 담체를 포함하는 높은 방사능의 화합물을 얻을 수 있다. 결과: IVDU와 IVFRU 표지를 위한 전구체 합성수율은 각각 43, 72% 였다. ICl로 담체를 이용한 표지방법을 사용하여 IVDU와 IVFRU 표지수율은 98% 이상이었다. 결론: HSV1-tk를 이용한 유전자 영상용 기질의 전구체를 성공적으로 합성하였고, 95% 이상의 높은 표지수율의 유전자 영상용 방사성 추적자를 성공적으로 제조하였다. Purpose: In gene therapy, tumor cells expressing the herpes simplex virus thymidine kinase are sensitive to prodrugs. Potential prodrugs IVDU and IVFRU were synthesized and radiolabeled with radiodine for noninvasive imaging of herpes simplex virus type 1 gene expression. Material and Methods: 5-(2-trimethysilyl)vinyl-2`-deoxyuridine and 5-t(2-trimethylsilyl-2`-fluoro-2`-deoxyuridine, precursors of 5-(2-iodo)viny 1-2`-deoxy uridine(IVDU) and 5-(2-iodo)-2`-vinyl-2`-deoxy-2`-fluororibofuranosyl uracil(IVFRU), were synthesized from reaction of trans-1-trimethylsillyl-2-tri-n-butylstannylethylene with 5-iodo-2`-deoxyuridine and 5-iodo-2`-fluoro-2`-deoxyuridine, respectively, on the condition of Pd catalyst. These precursors were separated from reaction mixture by silica gel column chromatography method. Each precursor was radioiodinated with radioiodine by mixing with ICl oxidizing agent. These radioiodinated compounds were purified with HPKC. Radiohalogen exchange has been shown to be effective for the synthesis of products with lower specific activity. Similarly, carrier-added and high specific activity products have been isolated in respectable radiochemical yields using ICl method. Results: Synthetic yield of precursors, IVDU and IVFRU were 43% and 18%, respectively. Radiochemical purity of both compunds was over 98%. Conclusion: We synthesized precursors of IVDU and IVFRU for monitoring of HSV1-tk gene expression. Radiotracers were radioiodinated with high radiolabeling yield by ICl method. (Korean J Nucl Med 2002;36;102-109)
안순혁(S.H.Ahn),윤면근(M.K.Yoon),박세영(S.Y.Park),조성철(S.C.Cho),류정인(J.I.Ryu) 한국자동차공학회 1995 한국자동차공학회 춘 추계 학술대회 논문집 Vol.1995 No.11_2
Today, modern society is an excessive consumption age of petroleum energy. An excessive consumption of petroleum fuel is accompanied with the panic of ultimate depletion of existing oil resources and environmental contamination by exhaust gas.<br/> Based on this situation, gasoline-methanol blends which is the mixture of gasoline and alcohol as well as compatible to existing spark ignition engine with minor or modification was developed. <br/> This is an experimental research to study the characteristics of spray for gasoline-methanol fuel with gasoline injector. To measure the droplet size(SMD) we used the Malvern system 2600c.<br/> The results of study are that in the physical properties, the specific gravity and viscosity of blending fuel increase, mean diameter of droplet(SMD) decrease as injection pressure increase and SMD increase in the increase of blending rate.<br/>