홍만병초 분획물에 의한 HT-29대장암 세포의 Wnt/β-catenin 신호전달 조절

심보람(Bo Ram Sim),남영선(Young Sun Nam),이자복(Ja Bok Lee) 한국생명과학회 2019 생명과학회지 Vol.29 No.8

본 연구에서는 Rhododendron brachycarpum (RB, 홍만병초)의 80% 메탄올 추출물 및 분획물의 항암 활성을 규명하고자 하였다. RB n-hexane 분획물은 HT-29 세포에서 가장 높은 활성 저해를 보였다(IC50 = 20.2±1.2 μg/ml). 더욱이, 콜로니와 구형 형성은 수와 크기는 유의적으로 감소시켰다. RB의 n-hexane 분획물에서(0.22±0.02 fold change) TOP / FOP 플래시 리포터 억제 활성은 추출물 및 다른 분획물 보다 낮게 나타났다. n-hexane 및 ethyl acetate 분획물은 세포 내 β-catenin의 발현을 조절하였다. 2 차 대사 산물이 β-catenin 분해를 감소시킬 수 있는지 여부를 조사하기 위해 Western blot을 실시한 결과 n-hexane 분획물에서 p-GSK3β를 조절하였으며, 세포내 β-catenin은 핵에서 정량적인 변화를 가져왔다. 이러한 결과는 RB의 n-hexane 분획물로부터 천연 항암 물질을 포함하고 있음을 보여줍니다. The aim of this study was to determine the anticancer activities of an 80% methanol extract and various fractions of Rhododendron brachycarpum (RB). The n-hexane fraction of RB showed the highest inhibitory activity (Inhibit concentration 50% = 20.2±1.2 μg/ml) in HT-29 cells. Colony- and sphere-forming abilities were significantly correlated with a decrease in the cell count and size. A TOP/FOP flash reporter assay revealed that the inhibitory activity of the n-hexane fraction of RB (0.22±0.02 fold change) was lower than that of the 80% methanol extract and that of other fractions. The n-hexane and ethyl acetate fractions of RB were predominantly dependent on the expression levels of intracellular β-catenin. Western blotting using p-GSK3β with only the n-hexane fraction of RB was conducted to examine whether these secondary metabolites reduced β-catenin degradation. Intracellular β-catenin regulation resulted in quantitative changes in the nucleus. In summary, these results demonstrate the potential of the n-hexane fraction of RB as a natural anticancer agent.

건강도시 양구의 아토피 없는 지역 만들기 프로젝트 사례 연구: 3년간의 성과

심보람 ( Bo Ram Sim ),남은우 ( Eun Woo Nam ),최응호 ( Eung Ho Choi ),박종남 ( Jong Nam Park ) 대한보건협회 2013 대한보건연구 Vol.39 No.2

Objectives: This study is intended to analyze the Atopy Free City project, which the Yanggu-gun started as a new approach about atopic dermatitis, and present its performance with suggestions. Methods: The target area is Yanggu-gun and relevant official documents carried out from 2008 through 2011 were analysed. Results: The result shows that the county established a community network and an advisory committee, and promoted the understanding of atopic dermatitis. The county also built an atopic dermatitis care village with ‘Healthy Woods Atopy Center’ where visitors can stay to not only treat atopic dermatitis but also get accurate health information and experience eco-friendly programs. Its “Atopic dermatitis camps” provide campers with enhanced, self-care skills. Conclusion: As part of the Healthy City Project, the Atopy Free City project provides various programs to people from Seoul and other cities, as well as the local residents. The project has established a foundation of eco-friendly, high-value-added industry, leading to enhancement of the brand image of the county. This case is a good example of a project carried out with the cooperation of a local government and a university program.

The Regulation of Early Senescence-related SM22 Protein in Human Dermal Fibroblasts by Hovenia dulcis Thunberg Fractions

Bo Ram Sim(심보람) 한국생명과학회 2020 생명과학회지 Vol.30 No.8

본 연구에서는 Hovenia dulcis Thunberg (헛개나무)의 80% 메탄올 분획물의 항산화 및 세포 노화 억제활성을 규명하고자 하였다. 헛개나무 에틸아세테이트 분획물이 DPPH라이칼 소거능에서 가장 높은 활성 저해를 보였고 (RC₅₀ = 3.6±0.8 μg/ml), 총 페놀(417.2±5.3 mg GAE/ml) 및 총플라보노이드(260.6±27.5 mg QE/ml) 측정에서도 가장 높은 활성을 보였다. HDF 세포에서 노화가 진행됨에 따라 sm22의 발현이 증가되었고(p<0.001), β-갈락토시 다아제의 염색양이 증가(p<0.001)되는 것을 확인하였다. 반면, sm22의 증가는 세포 노화가 진행된 세포일수록 증가폭이 감소되는 것이 확인되었고, 초기노화에서 발현이 높은 것을 알 수 있었다. 헛개나무 에틸아세테이트 분획물을 처리시 HDF세포의 EGF (p<0.01), PDGF-BB (p<0.01), SHH (p<0.05)의 사이토카인이 유의적으로 증가를 보이며, 노화 시 발생된 sm22의 수치를 감소시키는 것을 보였다. 이러한 결과는 헛개나무 에틸아세테이트 분획물이 초기 세포 노화의 작용을 조절할 수 있음을 보여준다. Several studies have proved Hovenia dulcis extracts to be an effective antidote for alcoholism, and they are frequently used in the clinical treatment of alcoholic liver disease. The purpose of this study was to investigate the anti-oxidant and anti-senescent activities of 80% methanol fractions of H. dulcis Thunberg in human dermal fibroblast (HDF) cells. The ethanol ethyl acetate fraction showed the highest inhibition of 2,2-diphenyl-1-picryhydrazyl radical scavenging (RC₅₀=3.6±0.8 μg/ml) as well as the highest total phenol content (417.2antiholic liver disease. Extracts from e & Tech., Sungkyunkwan University, progressed in the HDF cells, SM22 expression (p<0.001) and β-galactosidase staining intensity increased (p<0.001). An increase in SM22 expression is associated with slower aging which suggests that its expression is high in early aging. HDF treatment using the ethyl acetate fraction significantly increased production of EGF (p<0.01) and PDGF-BB (p<0.01) while SHH expression was reduced (p<0.05). These results suggest that an 80% ethyl acetate fraction of H. dulcis can suppress early aging and demonstrate the potential application of the fraction as a natural anti-senescence agent.

통영연안의 패류양식장 중단 이후 다모류 군집구조의 변화

박소현,김선영,심보람,박세진,김형철,윤상필,Park, Sohyun,Kim, Sunyoung,Sim, Bo-Ram,Park, Se-jin,Kim, Hyung Chul,Yoon, Sang-Pil 해양환경안전학회 2021 海洋環境安全學會誌 Vol.27 No.5

The recovery of the sedimentary environment around a fallowing shellfish farm during its rest period after 20 years of long-running operation, was explored in Tongyeong Bay of Korea. Seasonal survey on macrobenthos community was conducted from July 2016 to September 2018 at five stations, which included the fallowing shellfish farm after 20-year operation (FF), a newly installed shellfish farm (NF), a shellfish farm for comparison around the closed shellfish farm (CF), and control stations with no farming activities (Con1, Con2). The total number of species and the density of macrobenthos were higher at the shellfish farm stations than the non-shellfish farm station, and their seasonal changes were significant. The dominant species at the shellfish farm stations was Capitella capitata while Paraprionospio patiens was dominant at the non-shellfish farm stations. The two dominant species were potential organic contamination indicators usually inhabiting the areas with dense organic matter. This suggests that the overall survey area was highly polluted with organic matters. Multivariate analysis showed that the macrobenthic community of CF changed to a level similar to that of Con1 immediately after the investigation, but not to a state above the Con1 level. Furthermore, the changes in NF were not significant when compared to those in CF. 본 연구에서는 장기간 운영된 양식장에 휴지기가 주어졌을 때, 양식장 주변 퇴적 환경에서 나타나는 회복상태를 규명하고자 하였으며, 이를 위해 경남 통영의 수하식 굴양식장 일대에서 대형저서다모류 군집을 조사하였다. 2016년 7월부터 2018년 9월까지 20년간 운영된 후 폐쇄된 양식장(FF), 양식장의 이동으로 새롭게 설치된 양식장(NF), NF와 인접한 비교대상의 굴양식장(CF), 그리고 양식시설이 없는 두 개의 대조지역(Con1, Con2) 등 5개 정점에서 현장조사를 수행하였다. 출현종수와 서식밀도는 여름철에 감소하고 늦가을철 부터 증가하는 계절 변동을 보였으며, 양식장 정점들이 비양식장 정점들에 비해 높았다. 출현종의 상위우점종은 비양식장 정점에서는 Paraprionospio patiens, 양식장 정점에서는 Capitella capitata로 차이를 보였으나, 두 종 모두 유기물이 심한 지역에서 출현하는 잠재적 유기물 오염지시종으로 조사지역 저서환경의 유기물 오염이 상당히 진행되었음을 나타내었다. 다변량분석 결과, FF가 Con1과 군집구조가 유사한 수준으로 변화하였지만 Con1 이상의 상태로 회복을 나타내지 않았다.

옹진군 어장관리해역 해제 및 변경 타당성 평가

강성찬,김형철,황운기,심보람,김청숙,이원찬,홍석진,Kang, Sungchan,Kim, Hyung Chul,Hwang, Un-Ki,Sim, Bo-ram,Kim, Chung-sook,Lee, Won-Chan,Hong, Sokjin 한국수산과학회 2017 한국수산과학회지 Vol.50 No.5

Some of the mariculture grounds near Ongjin-gun, Korea, were designated as mariculture management areas in 2007. Areas are so designated when the environmental quality of the mariculture ground deteriorates or there is an outbreak of hypoxia or harmful red tide that kills mariculture organisms. We surveyed the water and sediment quality and examined the mortality of mariculture organisms in the Ongjin-gun mariculture area. In a survey conducted in 2016, the water quality was better than the environmental quality standards for mariculture grounds, excepts for dissolved inorganic nitrogen, and the sediment quality was good. However, there was still mortality of mariculture organisms in some of the designated management areas. The areas that met the environmental quality standards should be delisted and the areas in which we observed mortality should be classified as management areas. This will enable the sustainable development of aquaculture and preserve healthy mariculture grounds.

자란만 패류양식해역의 물리환경 설명을 위한 평균체류시간 산정

김진호,박성은,김영민,김청숙,강성찬,정우성,심보람,엄기혁,Kim, Jin Ho,Park, Sung-Eun,Kim, Youngmin,Kim, Chung Sook,Kang, Sungchan,Jung, Woo-Sung,Sim, Bo-Ram,Eom, Ki-Hyuk 한국환경과학회 2020 한국환경과학회지 Vol.29 No.3

Residence time is defined as the time taken for a material in a system to leave the system. The residence time characteristics in shellfish aquaculture determine the dispersion of excretion from aquaculture farms, along with the supply of food by seawater exchange. In this study, we estimated the spatial distribution of average residence time in the shellfish farming area using a particle tracking model. As a result, a relatively short average residence time of about 20 days or less was calculated in most areas, but an average residence time of more than 40 days was calculated in the inner areas. Relatively long average residence times were calculated along the west coast compared to the east coast, with the longest average residence time of more than 50 days in the northwestern areas. It can be inferred that the disturbance of the benthic ecosystem caused by shellfish farms is likely to be large because of the relatively weak dispersion of excrement from shellfish farms located on the west coast, especially in the northwest region. This distribution of average residence time is important for understanding the potential effects of seawater exchange on the environmental sustainability of shellfish farms, along with the seawater circulation characteristics of Jaran Bay.

필리핀의 사회적 자본, 보건의료이용 및건강생활 실천에 관한 연구

남은우(Eun Woo Nam),문지영(Ji Young Moon),김도형(Do Hyeong Kim),심보람(Bo Ram Sim),김춘배(Chun-Bae Kim),김영제(Young Je Kim),김판석(Pan Suk Kim) 국제개발협력학회 2014 국제개발협력연구 Vol.6 No.1

개발도상국의 보건의료 개선을 위한 국제협력사업 개발을 위해서는 그 지역 주민의 보건의료 이용현황은 물론이고 건강증진, 주관적 건강, 행복경험, 자살충동과, 수면의 상태 등과 같은 건강 생활 실천 현황과 사회적 자본 등의 파악은 물론 성별에 따른 차이를 규명할 필요가 있다. 이를 위하여 본 연구에서는 필리핀 마닐라 지역에 위치한 두 빈곤지역 주민을 대상으로 2011년도에 사회적 자본, 보건의료 이용 및 건강생활 실천 정도에 대하여 설문 조사하였다. 그 결과, 첫째, 성별에 따른 사회적 자본의 경우 친한 친구 수, 이웃과 자주 이야기 하는 비율 및 이웃을 자주 방문하는 비율 모두 남성이 더 높았다. 둘째, 성별에 따른 보건의료 이용 차이분석 결과, 1년간 필요시 의료서비스를 받지 못한 이유로는 남성은 시간이 없어서, 여성은 경제적 이유 때문이라는 응답이 가장 많았다. 셋째, 건강생활실천의 경우는 흡연율과 음주율이 남성 에서 더 높아 위해한 행동을 많이 하고 있었고, 반면에 규칙적 운동 실천률은 여성에서 낮았다. 지난 1년 동안 자살충동의 경우 남성이 더 높았고, 평균 수면시간의 경우 여성이 짧았다. 결론적으로 필리핀 주민의 사회적 자본 지수는 남성이 더 높았 으나, 높은 자살 충동, 흡연과 음주 등의 건강위험 행동을 더 많이 하고 있었다. 여성의 경우는 사회적 자본 지수가 낮고, 경제적인 이유로 인하여 필요시 의료서비스를 받지 못한 경우가 많았고, 낮은 운동 실천률, 적은 수면시간 상태에 있음을 알 수 있었다. 따라서 향 후 조사 대상지역과 유사한 지역 특성을 같는 빈곤지역의 보건사업을 개발할 때는 이러한 요소들을 고려하고 성별에 따라 접근방법을 달리하여 국제보건전문가 또는 보건교육사에 의한 사업 개발과 실행이 필요함을 시사한다. In order to develop international cooperation projects to improve health statuses in various developing nations as well as the medical treatments used, efforts should be made to understand the health and medical conditions, social capital, and gender relations of these nations. For this purpose, a survey was conducted in two impoverished areas of Metropolitan Manila, Philippines, in 2011. This survey studied the amount of social capital, the level of health care services, and the lifestyle choices of persons living in these areas. The survey showed that the social capital index was higher in male residents of the Philippines. However, it also showed that they were more likely to engage in harmful behaviors such as smoking and drinking. The likelihood of suicidal thoughts was higher in males as well. The survey also showed that because female residents have lower social capital, they are more likely to experience financial difficulties and thus are less likely to receive necessary health care services. In addition, they were shown to engage in less physical exercise and get less sleep than male residents. Thus, programs that are developed by international health specialists and health educators should consider elements such as those described above. Variety specialists who study public governance mechanisms should also assist in the creation of these programs because they understand the nuances of the topic at hand. We must integrate all three groups of people in order to develop effective health care programs in impoverished areas like the above-mentioned region.

감국의 유산균 발효물이 hedgehog 신호를 통한 지방구세포 분화 억제효과

최재영(Jae Young Choi),임종석(Jong Seok Lim),심보람(Bo Ram Sim),양영헌(Yung Hun Yang) 한국생명과학회 2020 생명과학회지 Vol.30 No.6

본 연구는 지방감소를 위한 소재개발로 감국 유산균 발효물이 갖는 지방구세포 분화 억제효과를 관찰하였다. 감국 추출물의 세포독성을 극복하는 유산균의 발효물을 제작하였다. 3T3-L1 세포주에서 감국 추출물 및 발효물이 갖는 세포독성은 모두 없었다(1day culture). 감국 추출물 처리 대조군과 비교하여 3T3-L1 세포주에 처리시 증식 유도된 발효물을 선별하였다. 감국 추출물 및 발효물의 분화억제 및 세포생존률 FACS분석은 분화 유도된 세포가 모든 실험군에서 줄어들었다. 3T3-L1 세포주에서 감국 추출물과 발효물 처리가 protein kinase B (Akt) pathway활성이 증가하였고, 단백질 발현은 지방구세포로 분화되면서 Gli2의 수준은 감소하였다. Hedgehog를 조절하는 유산균은 KCTC 3115인 것을 알 수 있었다. 분화와 관련된 KCTC 3115 및 KCTC 3109 발효군에서 단백질 수준에서 C/EBPα 및 FAS를 감소, pACC는 증가시키는 것을 확인하였다. 감국 추출물과 4개의 감국 유산균 발효물 중 Lactococcus lactis subsp. lactis KCTC 3115 발효물이 지방구세포 분화 신호를 더 효과적으로 조절하고, hedgehog을 같이 조절하여 지방전구세포의 분화를 억제하는 것을 알 수 있었다. Hedgehog 신호를 조절하면서 분화를 억제하는 물질에 대한 연구가 더 필요할 것으로 판단된다. 따라서 감국 발효물의 생리활성 물질 중 향후 매커니즘 분석을 위한 활성물질의 자료가 더 필요할 것으로 여겨지며, 감국 추출물 및 감국 발효물의 hedgehog 신호조절이 새로운 비만치료제로 개발될 수 있음을 위한 가능성을 제시하고자 한다. In this study, we describe the inhibition of adipocyte differentiation by the lactic acid bacteria (LAB) fermentation product of Chrysanthemum indicum L. (CI) extract to control obesity. Preparation of LAB-fermented products was performed to overcome the cytotoxicity of CI extract. During fermentation and 3T3-L1 cell line experiment, cytotoxicity was not induced in the CI fermentation products over 1 day in culture. Fermented materials from highly proliferative cultures were selected for treatment of 3T3-L1 cells and for comparison with unfermented control groups. Cell survival and undifferentiated cell populations were decreased differentiation population in all experimental groups compared with controls, as measured using fluorescence-activated cell sorting analysis. Akt pathway activity increased upon treatment with these fermented extracts in 3T3-L1 cells. Gli2 depleted at the protein level in association with adipocyte differentiation. LAB KCTC 3115- and 3109-fermented extract treatment caused controlled Gli2 protein accumulation. Moreover, KCTC 3115 and 3109 were found to reduce C/EBPα and FAS was depleted, whereas pACC was increased at the protein level upon treatment with the fermentation products of each of the four LAB used in this study. With Lactococcus lactis subsp. lactis KCTC 3115 fermentation, the regulation of adipose differentiation and hedgehog signaling were also suppressed, thereby inhibiting the differentiation of progenitor cells. The basis for the activation of hedgehog signaling may provide insights into the treatment of obesity and the inhibition of adipocyte differentiation.