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제초제 2,4-D가 흰쥐 소장 점액질에 미치는 영향에 관한 조직화학적 연구
신인성,정길남,조운복 부산대학교 사범대학 과학교육연구소 2007 科學敎育硏究報 Vol.34 No.-
제초제인 2,4-D가 십이지장샘, 십이지장, 회장 및 공장 술잔세포내 점액질에 미치는 영향을 규명하기 위하여, 성숙한 흰쥐에 제초제 2,4-D 액제를 경구적으로 투여하며 중독시킨 후 3시간군, 6시간군, 12시간군, 24시간군, 48시간군, 72시간군, 96시간군 및 120시간군 별로 나누어 점액샘 및 술잔세포내 점액질 성상을 alcian blue pH 2.5 염색법, alcian blue pH 1.0 염색법, PAS 염색법, alcian blue pH 2PAS 염색법, alcian blue pH 1.0-PAS 염색법 및 aldehyde fuchsin pH 1.7-alcian blue pH 2.5 염색법으로 검색하여 관찰하였다. 십이지장샘내 중성점액질 양은 농약투여 후 12시간군부터 96시간군까지 현저한 감소를 보였으며, 감소된 중성점액질 양은 농약투여 후 120시간군에서 회복되었다. 농약투여 후 소장 술잔세포내 점액질 감량은 음모상부 및 은와저부 쪽에서 더 심하였고, 산성점액질의 감량이 중성점액질의 감량보다 매우 더 심하였고, 회복도 산성점액질보다는 중성점액질쪽에서 더 빨랐다. 장기별로는 십이지장 술잔세포가 제일 심한 영향을 받았고, 다음 공장 배상세포이었으나 회장에서는 현저하지 않았다. 소장에 있어서 산성점액질의 가장 현저한 감소는 십이지장에서 농약투여 추 12시간군, 24시간군 및 48시간군 또는 공장에서 농약투여 후 24시간군과 48시간군이었으며 현저한 감소는 십이지장에서 농약투여 추 3시간군과 6시간군, 공장에서 농약투여 후 12시간군 그리고 다소 현저한 감소는 회장에서 농약투여 후 12시간군, 24시간군, 48시간군 및 72시간군이었다. 감소된 산성점액질 양은 십이지장 및 공장에서 농약투여 후 72시간군 또는 회장에서 농약투여 후 96시간군에서 점차적으로 회복되는 경향을 나타내었다. 중성점액질 양의 감소는 십이지장에서만 농약투여 후 12시간군, 24시간군 및 48시간군에서 현저한 감소를 나타내었으나 공장 및 회장에서는 중성점액질의 감소가 현저하지 않았으며, 감소된 중성점액질 양은 농약 투여 후 십이지장 및 공장에서 72시간군 또는 회장에서 96시간군에서 점차 회복되는 경향을 나타내었다. 소장융모 및 은와내 강 sulfated mucin을 분비하는 술잔세포와 sialomucin을 분비하는 배상세포가 농약투여 후 다같이 그 염색성이 감희되었고, 전자가 후자보다 더 심한 영향을 받았으며 회복되는 경향도 늦었다. 특히 공장에서는 sialomucin을 분비하는 술잔세포가 농약투여 후 12시간군, 24시간군 및 48시간군에서 증가되는 경향을 나타내었다. This experiment was performed to observe the effect of herbicide, 2,4-D, on the production, secretion and histochemical properties of the mucosubstances of the duodenal glands and goblet cells in the small intestine. Healthy adult rats weighing about 250~300g were divided into two groups normal and experimental. Each animal of experimental groups was administrated 2,4-D (187.5mg/kg) orally and sacrificed in 3, 6, 12, 24, 48, 72, 96 and 120 hours after 2,4-D administration. The mucous glands and the goblet cells of the small intestine were stained with alcian blue(AB) PH 2.5, AB pH 1.0, periodic acid Schiff (PAS), AB pH 2.5-PAS, AB pH 1.0-PAS and aldehyde fuchsin AF pH 1.7-AB pH 2.5. The obtained results were as follows : Marked diminution of neutral mucosubstances in the duodenal glands was noted up from 12 hours to 96 hours, and recovered gradually from 120 hours after 2,4-D administration. Decrease of both acid and neutral mucosubstances was noted to be considerable in the goblet cells of the small intestine after 2,4-D administration, especially of the upper willi and the basal regions of the crypts, and add mucins became more markedly diminished than neutral ones, while neutral mucins more strikingly recovered than add ones. In comparison with organs, e goblet cells of duodenum seemed to be most strikinly affected, next those of the ileum, but those of jejunum not affected markedly. The most remarkable decrease of acid mucosubstances was observed in the duodenum 12, 24 and 48 hours, in the jejunum 24 and 48 hours. Remarkable decrease of those observed in the duodenum 3 and 6 hours, in the jejunum 12 hours. Slight decrease of those observed in the ileum 12, 24,m 48 and 72 hours after 2,4-D administration. the decreased amount of acid mucins tended to be gradually recovered in the duodenum and jejunum 72 hours, in the ileum 96 hours after 2,4-D administration. The amount of neutral mucins was markedly diminished only in the duodenum 12, 24 and 48 hours after the administration, but neutral mucins was not diminished in the jejunum and ileum. The decreased amount of neutral mucins tended to be gradually recovered in the duodenum and jejumin 72 hours and in the ileum 96 hours after the administration. Stainability of the goblet cells with strong sulfated mucins and with sialomucins both in the intestinal villi and crypts was generally decreased after the administration. However, the former was more markedly affected than the latter. The tendency of recover appeared to be slow, especially the goblet cells with sialomucins in the jejunum 12, 24 and 48 hours tended to be increased after the administration.
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신인성,조운복 부산대학교 1982 자연과학논문집 Vol.34 No.-
This study is performed in order to clarify the histochemical properties of the mucosubstances in the doudenal glands, and striated border and goblet cells of the duodenum, jejunum, ileum, cecum, colon and rectum of squirrels. The specimens used in the study were obtained from the small and large intestines of healthy adult squirrels. The mucosubstances were stained with alcian blue pH 2.5, alcian blue pH 1.0, alcian blue pH 0.4, aldehyde fuchsin pH 1.0, periodic acid Schiff(PAS), alcian blue pH 2.5-PAS, alcian blue pH 1.0-PAS and aldehyde fuchsin pH 1.7-alcian blue pH 2.5 methods. The obtained results were as follows: 1. The histochemical compositions of mucosubstances on the striated border in the intestinal mucosa were different in each organs. These are in the small intestine, the inner layer of striated border contained mostly neutral mucin, and the outer edge contained the acid and neutral mucins, and the former was relatively more prominent than the latter. The acid mucin in the outer edge of the duodenum contained strongly sulfated mucin or less strongly sulfated mucin, but the outer edge of the jejunum and ileum was strongly sulfated mucin. The striated border of the large intestine was less conspicuous than that of the small intestine and contained equally both of the acid and neutral mucins, and in the cecum, the acid mucins of the striated border, consisted strongly sulfated mucin or less strongly sulfated mucin, but in the colon and rectum, the striated border had mostly strongly sulfated mucin. 2. In the small intestine, the duodenal glands contained small amount of neutral mucin. But the histochemical compositions and amounts of mucosubstances in the goblet cells of small intestine were in considerable variation in each portions. These are, the goblet cells of villi and crypts contained equally both of acid and neutral mucins, and the amounts of mucosubstances in goblet cells of the villi were much more than the amounts of those in the goblet cells of crypts. In villi and crypts of the duodenum and jejunum, two types of goblet cells were present: the one was contained strongly acidic sulfated and neutral mucins and the other mixed sulfated and carboxylic and neutral ones, and the fomer was more abundant than the latter. In villi and crypts of the ileum, on the other hand, three types of goblet cells were present: the contained nonsulfated and neutral mucins and the other mixed sulfated and carboxylic and neutral mucins, and the last strongly acidic sulfated and neutral ones. 3. The histochemical compositions and amounts of mucosubstances in the goblet cells of large intestine were varied with the portion differences. These were contained the acid and neutral mucins in the surfaces goblet cells of the colon and rectum and the surfaces and crypts goblet cells of the cecum, and the fomer was relatively more prominet than the latter in the colon and rectum, but the cecum were showed equally both of acid and neutral mucins. The crypts goblet cells of the colon and rectum were contained the acid and netral mucins, and the former was relatively more prominent than the latter. In crypts of the colon and rectum, two types of goblet cells were present: the one was contained acid mucin and the other acid and neutral mucins, and the former was more abundant than the latter. The amounts of mucosubstances in goblet cells of the surfaces and upper crypts were more prominent than amounts of those in the goblet cells of lower crypts. By the compositions of acid mucins in the goblet cells ofthe large intestine, the goblet cells of the surfaces and upper crypts were present three types of goblet cells: the one was contained strongly acidic sulfated and neutral mucins and the other mixed sulfated and carboxylic and neutral ones, and the last nonsulfated and neutral ones, but were contained only strongly acidic sulfated and neutral mucins on the goblet cells of the surfaces in the proximal colon and rectum. In the surfaces of the distal colon, on the other hand, were present two types of goblet cells: the one was contained strongly acidic sulfated and neutral mucins and the other less acidic sulfated and neutral ones, and the former was more abundant than the latter. And also the crypts of the colon and rectum were present two types of goblet cells: the one was contained only strongly acidic sulfated mucin and the other less strongly acidic sulfated and neutral ones, and the former was more prominent than the latter.
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전기화학적 방법을 이용한 다중 효소 전극 제작 및 글루코스 검출 특성에 관한 연구
한경호,신인성,윤도영 한국전기화학회 2020 한국전기화학회지 Vol.23 No.3
In this study, the development of biosensors capable of bi-enzyme reactions by including Horseradish peroxidase and glucose oxidase was carried out for detection of glucose. The sensors were manufactured using electro deposition method to reduce production time, and screen printed electrodes (SPE) were used to produce economical sensors. To check the bienzyme effect, the sensor was compared and analyzed with single enzyme biosensor. The characteristics of the sensor were evaluated using scanning electron microscopy(SEM), cyclic voltammetry( CV), electrochemical impedance spectroscopy(EIS), chronoamperometry(CA), and flow injection analysis(FIA). Analysis results from SEM, CV and EIS confirmed that the enzymes are well fixed to the electrode surface. In addition, it was confirmed that bi-enzyme biosensors manufactured from the CA method improved signal performance by 200% compared to single enzyme biosensors. From this results, we were able to explain that HRP and GOD react catalyzed to each other. And the results of FIA showed that the intensity of each current signal was constant when the same concentration of glucose was injected four times. In addition, by analyzing the intensity of current signals for glucose concentrations, the biosensors manufactured in this study showed excellent trends in signal sensitivity, reproducibility and stability.
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