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      • X 염색체-연관 Microsatellite의 Multiplex PCR Kit 제조

        신상희,한윤희,이경주,정기화 公州大學校 工學硏究院 生産技術硏究所 2003 論文集 Vol.11 No.-

        The microsatellite(short tandem repeat: STR) markers are widely dispersed in the human genome and have been extensively studied and applied to forensic analysis and human identification. The usefulness of the X chromosomal STRs for forensic purposes seems to be restricted, but may be valuable in paternity cases where the disputed child is female. In the present study, two kits of triplex PCR (Triplex 1: DXS6807, DXS6810 and DXS6789; Triplex 2: DXS7132, HPRTB and DXS9902) and one kit of duplex PCR(DXS1214 and DXS1227) were were deviced. Using the kits, population genetic data wereanalyzed for the six X-microsatellites in sample of 401 unrelated Koreans. The exact test for female genotyping data showed no significant deviation from the Hardy-Weinberg equilibrium(P≥0.01). Details of X chromosomal STRs in Koreans would be useful in paternity tests and forensic purposes as well as whole X-chromosomal mapping studies

      • KCI등재

        꿀벌의 독에 의한 생쥐 피부의 조직병리학적 및 미세구조적 변화

        신상희,정문진,문명진,Shin, Sang-Hee,Jeong, Moon-Jin,Moon, Myung-Jin 한국현미경학회 1997 Applied microscopy Vol.27 No.2

        꿀벌(Apis mellifera)의 독에 의해 야기되는 포유동물 피부의 조직병리학적 및 미세구조적 변화와 그 수복과정을 확인하기 위하여 실험용 생쥐의 피부에 직침법으로 꿀벌의 독을 주입한 후, 회복된 시점까지 일정시간 간격으로 조직의 표본을 제작하여 광학 및 전자현미경으로 관찰하였다. 독 주입 직후의 표본에서는 표피의 상피세포와 진피의 결합조직에서 현저한 염증반응이 유도되었고, 일부세포의 괴사가 관찰되었다. 고배율의 전자현미경상에서 교원섬유의 직경이 크게 증가되었으며, 면역 단백물질로 추정되는 전자밀도가 높은 grain의 침착이 확인되었다. 이러한 조직병리현상은 독 주입 후 12시간이 경과된 조직의 표본에서 서서히 회복되는 것으로 관찰되었다. 봉침 주위 피부조직의 조직학적 및 미세구조적 변화는 수 일간 지속되었으나, 병리학적 반응은 3일 이내에 거의 거의 소멸되는 것으로 관찰되었다. 또한 생쥐 피부에 대한 꿀벌 독의 병리반응은 다른 절지동물의 독에 비하여 비교적 경미한 것으로 확인되었다. Histopathological and fine structural changes in mouse skin after injection of venoms extracted from the venom glands of the honeybee, Apis mellifera, were studied with light and electron microscopes. At this experiment the venoms were directly injected at the hairless abdominal skin of the mouse through the sting of the bee's venomous organ. Main changes appeared within one hour after injection at both epithelial and connective tissues as considerable hyperemia and angioedema, and slight edema and fibrosis. High magnified electron micrographs reveal not only increase of diameter but also deposition of electron dense grains (which seems to be an auto immunoglobulin) at the collagenous fibers characteristically. This kinds of histological and fine structural responses were diminished from 12 hour after injection, and the pathological symptoms disappeared within 3 days at most cases. So, the skin responses induced by honeybee venom seem to be not severe compare to other cases reported by other venomous arthropods.

      • KCI등재

        소비자의 블로그 활용이 구매의사결정과정과 구매 후 행동에 미치는 영향에 관한 연구-화장품 소비자를 중심으로-

        신상희,황복주 한국경영교육학회 2015 경영교육연구 Vol.30 No.4

        Today, more consumers rely on social networking sites, such as blogs, to help them decide on what products to buy and which brand is best suitable for their needs. With that said, most cosmetic consumers turn to product reviews on blog sites to help them decide on which cosmetic brand fit their needs. This study focuses on the effects of consumers’ purchase decision-making and post-purchase behavior after reading certain cosmetic product reviews on blog sites. This study also enlightens cosmetic companies on the implications that results in their blog marketing plan. 178 cafe users were part of the analysis, according to a blog survey. The purpose of this survey is to determine how much and how often consumers rely on cosmetic product reviews on blog sites before they decided on which product to buy. Further results showed that more consumers utilize blogs as a big part of their cosmetics search tool. Consumers with blog sites post their own cosmetic reviews for others to read and comment on, resulting in a friendly relationship between bloggers and cosmetic consumers. In short, blog cosmetic reviews are a big influence on consumers’ purchase decision-making process. The following is a summary of this study’s result: The blog cosmetic reviews seem to arouse consumer interests on certain cosmetic brands. Consumers rely more on product reviews than simple product description. Consumers today prefer the honest blog product reviews instead of the biased professional reviews. Many consumers use blog product reviews as a great way to find new cosmetic brands that will fit their needs and budget. Consumers evaluate cosmetic products through blog product reviews. Furthermore, blogs help consumers decide which cosmetic brands best fit their needs and budget. The pros and cons of cosmetic product reviews on blog sites help consumers make the right purchase. Cosmetic product satisfaction, recommendation and re-purchase rates from blog product reviews significantly influence consumers’ purchase decision-making. Cosmetic product reviews from blog sites have helped create a positive image on purchased cosmetic brands. 스마트기기를 활용한 SNS의 사용은 새로운 흐름을 만들어내고 변화의 흐름을 빠르게 만들었다. SNS는 온라인 구전 마케팅 수단으로 이용되고 있으며 정보 생성과 확산이 빨라 소비자에게 큰 파급효과를 미치고 있다. 화장품과 같은 경우 다양한 컨텐츠를 담은 블로그 후기가 많이 작성되고 있다. 본 연구는 화장품 소비자가 블로그를 활용할 때 각 구매의사결정과정과 구매 후 행동에 어떠한 영향을 미치는지 연구하여 화장품 기업의 블로그 마케팅 방안에 대해 시사점을 제공하고자 한다. 이를 위해 블로그, 카페 이용자 178명을 대상으로 분석하였다. 본 조사는 화장품 소비자의 평소 블로그 활용 정도와 구매의사결정 시 블로그를 어느 정도 활용하는지에 대해 조사하였다. 연구결과에 따르면 화장품 소비자들은 구매를 위한 검색의 도구로서 블로그를 소극적으로 활용하는 경우가 많은 것으로 나타났으며, 다른 사람의 글에 댓글을 달거나 관계를 형성하는 것보다는 직접 블로그에 글을 올리는 경우가 더 많은 것으로 나타났다. 분석 결과, 화장품 소비자들의 블로그 활용은 구매의사결정 전반에 걸쳐 영향을 미치는 것으로 나타나 화장품 기업들은 각 기업들의 상황과 브랜드 이미지에 따라 블로그 마케팅 방법을 달리해야할 필요성이 제기 되었다.

      • KCI등재후보

        Effects of Specific Exercise on Chronic Neck Pain in Elderly Women

        신상희,최종덕 대한물리치료학회 2010 대한물리치료학회지 Vol.22 No.3

        Purpose: The main causes of chronic neck pain (CNP) are wrong postures and degenerative changes. This study investigated the effects of specific exercise on elderly women with CNP. Methods: 29 elderly women who complain of CNP were classified into experimental group (14) and control group (15). The experimental group was treated with specific exercise and minimal conventional therapy, whereas the control group was treated only with conventional therapy. Then the effects on the visual analogue scale (VAS), neck disability index (NDI), self-rating anxiety scale (SAS), Korean form of geriatric depression scale (KGDS), and range of motion (ROM) were compared between the two groups. Results: The experimental group showed significantly improved results in VAS, NDI, SAS, KGDS, and ROM after intervention (p<0.05), as did the control group (p<0.05). The comparison of changes in the experimental group before and after intervention showed superior results in the SAS, NDI, and ROM (flexion, rotation, lat. flexion) results when compared with the control group (p<0.05). Conclusion: Specific exercise is effective in the improvement of SAS, NDI, and ROM for elderly women with CNP. Purpose: The main causes of chronic neck pain (CNP) are wrong postures and degenerative changes. This study investigated the effects of specific exercise on elderly women with CNP. Methods: 29 elderly women who complain of CNP were classified into experimental group (14) and control group (15). The experimental group was treated with specific exercise and minimal conventional therapy, whereas the control group was treated only with conventional therapy. Then the effects on the visual analogue scale (VAS), neck disability index (NDI), self-rating anxiety scale (SAS), Korean form of geriatric depression scale (KGDS), and range of motion (ROM) were compared between the two groups. Results: The experimental group showed significantly improved results in VAS, NDI, SAS, KGDS, and ROM after intervention (p<0.05), as did the control group (p<0.05). The comparison of changes in the experimental group before and after intervention showed superior results in the SAS, NDI, and ROM (flexion, rotation, lat. flexion) results when compared with the control group (p<0.05). Conclusion: Specific exercise is effective in the improvement of SAS, NDI, and ROM for elderly women with CNP.

      • KCI등재

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