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電氣傳導度 및 Triphenyl Tetrazolium Chloride를 利用한 차나무 耐凍性 檢定方法 確立
천종은(J.U. Chun),강석원(S.Sw. Kang),송동석(D.S. Song),최정(J. Choi) 한국차학회 2000 한국차학회지 Vol.6 No.3
Simple reproducible procedures are described for assessing freezing injury of tea leaf involving controlled freezing of excised leaves and measurements of leached electrolytes and reduction of 2, 3, 5-triphenyl tetrazolium chloride (TTC) to a water~insoluble red formazan. The results showed that two methods had highly significant linear regression (R² = 0.757), suggesting TTC test had an advantage that a small amount of tissue (30~80 mg) is required. The two methods can be used in selection and breeding for freezing resistance in tea.