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Agrobacterium을 이용한 油菜(brassica napus)의 形質轉換
Jae Keun Sohn(孫再根),Sung Hee Kim(金承嬉),Kyung Min Kim(金敬旻) 한국육종학회 1994 한국육종학회지 Vol.26 No.1
The addition of AgNO₃(ethylene inhibitor) in MS medium remarkably enhanced the shoot regeneration from the cotyledonary petioles. The maximum frequency of shoot regeneration(74.2%) was obtained from the cotyledonary petioles cultured in the medium with 3㎎/ℓ AgNO₃. The presence of AgNO₃(3㎎/ℓ) also enhanced the shoot regeneration from the calli of hypocotyl and cotyledon tissues. The cotyledonary petioles cocultivated with Agrobacterium vectors for 72hours were transferred to MS medium with 0.5㎎/ℓ NAA, 2.0㎎/ℓ BA, 30㎎/ℓ kanamycin, 100㎎/ℓ cefotaxime, 30g/l sucrose, 3㎎/ℓ AgNO₃ and 2g/l gelrite. The cotyledonary petioles transferred to the selection medium formed green shoot with 17.5% efficiency. The putative transgenic plants were also obtained from the hypocotyl- and cotyledon-derived calli of B. napus. Southern blot analysis was done on the putative transformed plants’ genomic DNA digested with EcoR I and Hpa I restruction endonucleous. Agrobacterium-mediated transformation in B. napus was confirmed by hybridization with rol C and NPTⅡ gene(1.0kb and 2.0kb) as probes.
벼의 callus 培養에서 再分化된 植物體의 主要 形質 變異
Jae Keun Sohn(孫再根),Oh Hee Kwon(權五熙),Sam Taek Cheong(鄭三澤),In Koo Rhee(李麟九) 한국육종학회 1991 한국육종학회지 Vol.23 No.3
Somaclones regenerated from callus culture of a japonica rice cultivar, “Chucheongbyeo”, were evaluated for variation in nine agronomic and morphological characters ; heading date, grain fertility, culm length, panicle length, number of panicles per hill, number of grains per panicle, grain weight, amylose content and leaf color. The R₂ pedigree lines were derived by self-pollination of 196 plants(R₁) regenerated from primary callus. The calli were induced from mature seeds of an anther-derived plant. Only 24% of 196 R₂ lines were normal in all characters observed, 50% of the total lines were uniform within each line although the differences among lines were recognized, and 51 R₂ lines(26%) were segregated in one or more characters investigated. The proportion of R₂ lines carrying one or two mutated characters was 58.6% of total R₂ lines tested. The R₂ populations appeared to have shorter culm and panicle length, less effective tillers, and more grains per panicle as compared with mean values of original cultivar. However, the means of heading date, grain weight and amylose content were nearly the same as their respective checks.
벼 葯培養에서 캘러스 形成과 植物體再分化能力에 대한 遺傳分析
Jae Keun Sohn(孫再根),Sae Jun Yang(楊世準) 한국육종학회 1993 한국육종학회지 Vol.25 No.2
Inheritance of callus formation and plant regeneration in rice anther culture was studied using the diallel technique. Anthers containing uninucleate microspores from four cultivars(japonica ; “Chuncheongbyeo” and “Palgongbyeo”, Tongil ; “Chilseongbyeo”, indica ; “IR50”) and their F₁’s were cultured on N₆-Y₁ medium supplemented with 1.0㎎/ℓ NAA and 2.0㎎/ℓ kinetin. The one-step culture method of anthers using the culture bottle was applied for standardization of anther culture process in this study. The parents showed significant differences in frequency of callus formation, green plant regeneration and albino occurrence. General and specfic combining abilities were estimated and found to be highly significant for the three traits. Reciprocal effect was also significant for callus formation and albino occurrence, but it was not significant for green plant regeneration. Further analysis for green plant regeneration showed gene action to be overdominant with high response being highly recessive and controlled by a few genes. The estimates of narrow sense heritability for albino occurrence(0.32) was lower than those of callus fromation(0.76) and green plant regeneration(0.55).
Jae Keun Sohn(孫再根),Jeong Suk Bae(裵丁淑) 한국육종학회 1995 한국육종학회지 Vol.27 No.4
Immature cotyledons of soybean, Glycine max (L.), were cultured to improve the efficiency of somatic embryogenesis. Varietal differences for the capacity of embryo formation were evaluated to select a promising cultivar with high efficiency of somatic embryogenesis. Embryogenic capacity was strongly influenced by genotypes of donor plants. A Korean soybean leading cultivar, Danyeobkong, was identified to have the highest frequency(28.7%) of somatic embryogenesis among nine soybean accessions. Efficiency of embryogenesis was also varied with the developmental stages of soybean seed serving as explant sources. The maximum frequency(16%) of embryogenesis was obtained from the immature seeds with size of 4~6㎜. Addition of 2, 4-D in the medium enhanced somatic embryogenesis from immature cotyledons, but it inhibited the formation of normal somatic embryos. The induction frequency of somatic embryo with normal morphology was higher in the presence of NAA(10㎎/L) than that of 2, 4-D. Most of somatic embryos formed on medium with 2, 4-D were developed to abnormal types. Somatic embryogenesis from immature cotyledons was promoted in the presence of 3% sucrose.
Jae Keun Sohn(孫再根),Gi Hwan Yi(李起煥),Byong Geun Oh(吳秉根),Sang Jong Lim(林尙鍾) 한국육종학회 1995 한국육종학회지 Vol.27 No.4
Spontaneously doubled-haploid plants (A₀) were recovered from anther culture of japonica rice cultivar “Hwayeongbyeo” which developed by anther culture techniques. Culm length, panicle length, and 1,000-grain weight were compared with those of the donor plants for the determination of variability in A₁ and A₂ generation. Sixty-three percent of 52 A₁ lines were similar to the donor plants, while 21.1% were putative homozygous mutants that had no segregation within the lines and 15.4% showed heterozygous variants in one or more characters. One hundred and fifty seven A₂ lines derived from 33 A₁ lines which were simmilar to the donor plants in A₁ generation showed no significant difference in culm length, panicle length, heading date, and 1,000-grain weight. However, 114 descendents (A₂) of 19 lines varied in A₁ generation showed higher coefficient of variation than those of the donor cultivar “Hwayeongbyeo” in investigated characters, espacially in 1,000-grain weight.
Agrobacterium 을 이용한 Indica 형 벼 형질전환 효율개선
손재근(Jae Keun Sohn),정은모(Eun Mo Jeong),권용삼(Yong Sham Kwon) 한국육종학회 2002 한국육종학회지 Vol.34 No.3
To improve the transformation efficiency by Agrobacterium tumefaciens mediation in indica rice, we used calli derived from immature and mature embryo. Calli formed from immature embryos produced more transgenic plants than those from mature embryos. The m
장재기(Jae Ki Chang),손재근(Jae Keun Sohn),김호영(Ho Yeong Kim),김경민(Kyung Min Kim) 한국육종학회 1997 한국육종학회지 Vol.29 No.4
The diallel analysis was conducted with F₁ and F₂ population derived from crossing in all combinations without reciprocals among six rice cultivars, three tropical japonica and three japonica types, with different panicle number and panicle length. The expression was controlled by additive gene action with partial negative dominance in panicle number and partial positive dominance in panicle length. Epistasis was not recognized for the two characters. The narrow-sense heritability was estimated at 92% for panicle number and 82% for panicle length. GCA effects were larger than that of SCA in both characters. There were significant correlations between F₁ and F₂ generation on the ranks of parents GCA effects.
이현숙,김경태,손재근,김경민,Lee, Hyeon-Suk,Kim, Kyung-Tae,Sohn, Jae-Keun,Kim, Kyung-Min 한국식물생명공학회 2006 식물생명공학회지 Vol.33 No.2
Rot 3 유전자로 형질전환된 들깨를 이용하여 aromatic compounds, brightness, 안토시아닌 함량, leaf index를 조사하여 모본과의 품질적인 차이가 있는가를 조사하여 형질전환체의 안정성을 확보하기 위한 연구이다. Rot 3 유전자는 T1 과T2 세대에서 유전자발현이 되었으며, 형질전환이 확인된 개체는 주요 농업형질이 형질전환 되지 않은 들깨와는 차이가 없는 것으로 나타났다. 또한 aromatic compounds와 leaf brightness도 차이를 나타내지 않았다. 그러나 leaf index는 유전자의 표현을 나타내어 형질전환체와 형질전환되지 않은 들깨잎의 모양은 차이가 나타났다. This study was carried out to establish genetic transformation of Rot 3 gene into perilla plants and to evaluate aromatic compounds, brightness, anthocyanin contents and leaf index in Rot 3 overexpressing transgenic lines. Rot 3 transmitted successfully from T$_1$ to T$_2$ generation showing stable gene expression. It revealed that there was no difference between transgenic and non-transgenic plants in major agronomic characteristics of progeny analysis. There was not much difference in aromatic compounds and leaf brightness did not showed variations between transgenic and non-transgenic, but leaf index was distinguished, respectively.