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孫成源,安永泰 경남대학교 환경문제연구소 1987 환경연구 Vol.9 No.-
The dirstribution pattern of the distoma (Fasciola hepatica) which is Parasitic on the livestock and man was surveyed in Kyung nam area with 3608 samples of cattle. The results were as followings. The overall infection rate was 4.9%, as 6.5%, 3.4%, 4.0%, and 9.0% for the samples collected in Masan, Ulsan, Kimhae, and Chinju area respectivelly. The infection rate of the Fasciola hepatica was analysed by raising method, aga, sex, season, and infected organ. The analysis by rasing method showed that the infection rate of the beef-cattle raised mainly with commercial feed was only 0.6 per cent while that of the farm-cattle raised mainly by pasturage was 34.4 per cent. The analysis by age showed the increasingly high infection rate with aging, i. e. no infection of 1~2 years samples, 2.4% of 3 years ones, 26.2% of 6 years ones, and 42.9% of 7 years ones. Strangely 8 years samples showed only 14.3% infection. The analysis by sex showed the infection rate of 94.8 per cent for females and 5.2 per cent for males. The analysis by season showed the rate of 4.6 % for summer samples (Jul. ~ sep.) and 9.0% for winter ones (Feb. ~ Mar.). The analysis by infected organ showed that the highest infection rate of 77.4% for the gall duct and the lowest of 2.8% for the blood vessels.
Spermiogenesis in the Korean Daubenton's Bat(Myotis daubentonii ussuriensis)
손성원 한국발생생물학회 1997 발생과 생식 Vol.1 No.1
To investigate the process of spermiogenesis of the Korean eastern Daubenton's bat, Myotis daubentonii ussuriensis, the testis obtained from mature male bats was studied by transmission electron microscope and were based on the variety and diagnostic characters of cell organells. The results obtained from the present study are as follows. According to the differentiation of the cell organells, the spermiogenesis of the Korean eastern Daubenton's bat, M. d. ussuriensis, was divided into Golg, cap, acrosome, maturation and spermiation phases. Besides, these Golgi, cap, acrosome, and maturation phase were subdivided into the steps of early and late phases repectively and matruation phase was subdivided into step of early, mid and late phases. Therfore, the spermiogenesisof M. d. ussuriensis has been divided into a total of 11 phases. The chromatin granules began to condense at the early cap phase, regularized at the acrosome phase, and a perfect nucleus of sperm was formed at the maturation phase. The chromatoid body was occurred in the upper cytoplasm of nucleus at the early Golgi phase, and it was accurred the posterior cytoplasm of the nucleus at the early maturatio phase. The formation of sperm tail began to be develop in the early golgi phase, and completed at the spermiation phase. The fiber structure of middle piece was consisted of nine outer doublets and two central singlet microtubules and Nos. 1, 5, 6 and 9 in the outer dense were larger than the others(2, 3, 4, 7, 8).
손성원 한국발생생물학회 1997 발생과 생식 Vol.1 No.2
To study the function and structure of Golgi apparatus in the spermiogenesis of long-fingered bat (Miniopterus schreibersi fuliginosus), the testis obtained from adult bat was treated with the prolonged osmification or fixed with ferrocyanide reduced osmium. golgi apparatus was oval shape in early Golgi phase, and was composed of cortex and medullar enclosing acrosome in mid Golgi phase. The vesicles of crescent shape Golgi apparatus were closed or fused with small or large vesicles at the periphery of acrosome. Golgi apparatus moved behing the acrosome face in cap phase, but the Golgi apparatus was still active. According to this, Golgi apparatus appears to be involved in the formation of acrosome and sperm tail. Transfer of materials from Golgi to acrosme seems to be carried out not only by fusion of large vesicles with acrosomal vesicles but also by detachment of coated vesicle from various cisternae of Golgi fusing with acrosomal vesicle.
한국 희귀 특산식물 꼬리말발도리 집단의 유전적 다양성 및 구조
손성원,최경수,박규태,김은혜,박선주 한국자원식물학회 2013 한국자원식물학회지 Vol.26 No.5
Deutzia paniculata Nakai is a Korean endemic species that has a very restricted distribution in Gyeongsang-do,South Korea. The genetic diversity and structure of five populations of D. paniculata were investigated using 31 ISSR locifrom six primers. The Shannon’s index (0.429) and genetic diversity (0.271) were relatively higher than those of other rareplant species in Korea. The Miryang (MY) and Yangsan (YS) populations, which have higher flowering rates than the otherpopulations, showed greater genetic diversity than the other populations. An analysis of the molecular variance (AMOVA)showed that 16% of the total variation could be attributed to differences among the populations, and 84% to the differenceswithin populations, indicating moderate gene flow among adjacent populations. The high genetic diversity and low geneticdifferentiation in the Deutzia paniculata populations, which have a restricted distribution, is considered to be affected byoutcrossing of the mating system and abundant individuals in the populations. These results suggest that ex situ conservationstrategies are needed to sustain the current genetic diversity of D. paniculata. 꼬리말발도리(Deutzia paniculata Nakai)는 전 세계적으로우리나라 경상남북도 일부지역에만 자생하는 매우 제한된 분포범위를 가지는 특산식물이다. 이러한 꼬리말발도리 집단의 유전적 다양성 및 구조를 조사하기 위해 5집단 155개체에 대한ISSR(Inter Simple Sequence Repeat) 분석이 수행되었다. 총 6개의 ISSR 프라이머를 이용하여 31개의 증폭산물을 관찰하였으며, 집단 수준에서의 유전적 다양성의 평균은 SI(Shannon’sinformation index)=0.429, h (Nei’s genetic diversity)=0.271로, 매우 높은 수준으로 나타났다. 집단별로는 큰 차이는 없었지만 비교적 높은 개화율을 보이는 밀양, 양산 집단이 다른 집단에 비해 다소 높은 유전 다양성을 유지하고 있는 것으로 나타났다. AMOVA 분석 결과 전체 유전변이의 약 16%가 집단 간 차이에 기인하는 것으로 설명되었으며, 나머지 84%는 집단 내 개체간에 존재하는 것으로 나타났다. 이처럼 제한된 분포범위를 가지는 꼬리말발도리 집단에서 나타나는 높은 유전다양성과 집단간 낮은 유전적 분화율은 완전 타가수정하는 교배양식과 집단내 비교적 풍부한 개체수의 영향인 것으로 판단된다. 따라서 현재의 유전다양성을 유지할 수 있는 적절한 현지 내 보전대책 수립이 요구된다.
A new record of fern species from Korean flora: Asplenium septentrionale (L.) Hoffm. (Aspleniaceae)
손성원,이한권,양형호,이강협,김성식,권혜진 한국식물분류학회 2013 식물 분류학회지 Vol.43 No.2
Asplenium septentrionale (L.) Hoffm., belonging to the family Aspleniaceae, was recently recorded for the first time in Gyeongsangbuk-do, Uljin-gun, Buk-myeon, Docheon-ri. This species is distributed in Northern America, Europe, and Asia. This species, which is related by taxa of Asplenium, is distinguished in that it possesses a leaf blade that is more or less dichotomously divided, forked 1-2(-3) times, and narrowly linear (5-20 mm length) sori. The newly given Korean name, ‘Sol-ip-go-sa-ri’ reflects the presence of a linear leaf similar to Korean pine needles. A description of the key characteristics, an illustration, and photographs of the habitats of this plant are provided in this report.
희귀·특산식물 매미꽃의 분포와 자생지 식생특성 및 보전 지위 평가
손성원,정재민,신재권,이병천,박광우,박선주 한국식물분류학회 2012 식물 분류학회지 Vol.42 No.2
The distribution, vegetation characteristics and an assessment of the conservation status of the Korea endemic species Coreanomecon hylomeconoides Nakai were investigated to collect biological basic data to formulate a conservation strategy. According to the distribution map of C. hylomeconoides based on the literature, specimen information, and local field surveys, natural populations of C. hylomeconoides are intensively distributed in the Southern regions of the peninsula, around Gyeongsangnam-do and Jeollanan-do. C. hylomeconoides was distributed in the middle and low slope adjacent to a valley. The altitude ranged from 227 m to 744 m, with inclinations of 5-10o. As a result of a vegetation survey within natural populations, a total of 238 taxa were identified from 29 quadrates in 8 natural populations. The importance value of C. hylomeconoides is 25.34% based on the coverage and frequency of the herbaceous layer with in the populations. The species diversity of the occurrence of the species in 8 natural populations was 1.52, while the averages of species evenness and the dominance values were calculated to be 0.83 and 0.17, respectively. As a result of assessing the conservation status through IUCN Red List criteria, C. hylomeconoides was evaluated as Near Threatened (NT). Conservation strategies are also discussed for the sustainable conservation of C. hylomeconoides. 한반도 특산식물인 매미꽃 자생 집단의 보전대책 수립을 위한 기초자료로 활용하고자 자생 집단의 분포와 식생환경 및 보전지위 평가를 수행하였다. 문헌자료 및 표본정보 수집과 현지조사를 통해 분포도를 작성한 결과 매미꽃은 지리산과 백운산 등 경상남도와 전라남도를 중심으로 주로 한반도 남부지방에 집중 분포하는 것으로 확인되었다. 또한 자생 집단은 주로 해발 227~744 m의 범위에 분포하였으며, 경사는 5~11o계곡의 중·하부 지역에 많이 분포하였다. 8개 자생 집단에 대한 식생조사 결과 29개 조사구에서 총 238분류군이 출현하였으며, 각 조사구 내 초본층의 피도와 빈도를 기초로 매미꽃의 중요치를 산출한 결과 평균 25.34%로 나타났다. 8개 집단 내에 출현 종의 다양도는 평균 1.52였으며, 균등도와 우점도의 평균은 각각 0.83과 0.17로 산출되었다. 수집된 생물학적 자료를 바탕으로 IUCN 적색목록 기준에 의한 보전 지위를 평가한 결과 매미꽃은 Near Threatened(준위협종)에 포함되는 것으로 나타났으며, 이에 따라 매미꽃 자생 집단의 지속가능한 보존을 위한 전략수립과 필수적인 기초연구의 내용 및 방법이 논의 되었다.