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원심분리법을 이용한 다양한 형태의 조직공학용 다공성 알지네이트 지지체의 제조 및 분석
오세행,이진호,석경록,박일규 한국생체재료학회 2003 생체재료학회지 Vol.7 No.1
In recent years, several techniques have been used to fabricate porous polymeric scaffolds having 3-dimensional pore structure. However, it is not easy to fabricate the porous scaffolds with complicated shapes by conventional methods and thus the prepared scaffolds usually have simple shapes, such as sheet, disc, cylinder and tube. In this study, we developed a new porous scaffold fabrication method (centrifugation method) to prepare scaffolds with uniform surface and interior pore structures as well as various shapes. The porosity and pore size of the scaffolds could be controlled by adjusting centrifugation speeds. The pore sizes of the prepared alginate scaffolds decreased with the increasing centrifugation speed: as the centrifugation speed increased from 1,000 rpm to 3,000 rpm, the pore size of the scaffolds decreased from 250 m to 130 m. The alginate scaffolds were impregnated into chitosan solution with the different concentration (0~1.0 wt%) to improve mechanical strengths as well as cell adhesiveness. The maximum load and modulus analog of the scaffolds gradually increased by the chitosan impregnation. This study demonstrate that the centrifugation method is an effective method for preparing porous 3-dimensional scaffolds with complicated shapes for tissue engineering applications.
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