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사람 탯줄로부터 추출된 Type I Collagen의 Telopeptide 제거에 대한 분석
서활,안수진,김요숙,이하규,Suh, Hwal,Ahn, Sue-Jin,Kim, Yo-Sook,Lee, Ha-Gyui 대한의용생체공학회 1996 의공학회지 Vol.17 No.3
Although collagen is still considered to be a poor immunogen, animals can produce antibodies to a number of different sites in the collagen molecule. In type I collagen, three classes of antigenic determinants have been described those are recogrlized as different degrees in different species. These are essentially composed of helical, conformation-dependent antigenic determinants and terminal, nonhelical antigenic determinants, and finally central antigenic determinants exposed only after denaturation of the collagen molecule. To utilize collagen as implantable biomateriall human e61bryonic collagen, ten immunological to body, was purified from human umbilical cords and found to contain [$\alpha$1(I)]$_2$. [$\alpha$2(I). Each step of purification were observed by polarized light microscope and analyzed through SDS-PAGE. The conclusious are follows; 1 . The purified collagen revealed gradual fiber indenties on each step of purification by polarized microscope. 2. The structual changes of extracted collagen as removed telopeptide were confirmed by SDS-PAGE.
Type I Atelocollagen의 가교형성비 분석
안수진,김요숙,서활,Ahn, Soo-Jin,Kim, Yo-Sook,Suh, Hwal 대한의용생체공학회 1996 의공학회지 Vol.17 No.4
To utilize collagen as an implantable biomateriall the mcct widely used bovine skin origin Type I collagen was investigated Pepsin treated, Type I atelocollagen was extracted and crosslinked by the ultraviolet(W) ray with wavelength of 254nm or by various concentrations of glutaraldehyde to produce collagen membranes. The crosslink rates of the specimens were observed by a polarized light microscope, a scanning electron microscope, and a Fourier transform infrared (FT-lR) spectrometer. The followings are concluded 1. The collagen membranes produced by both 2.5% glutaraldehyde solution and 254nm UV ray irra- diation demonstrated similar morphologies on polarized light microscopic and scanning electron microscopic views. 2. The chemical structures of the crosslinked membranes by glutaraldehyde over 2.5% in concentrations revealed similar intensities to that of the UV ray irradiated one in FT-lR investigation. 3. To obtain optimal croulink in bovine stalin origin Type I atelocollagen, 2.5% glutaraldehyde solution or UV ray irradiation with 254nm wavelength is acceptable.
박종철,김경태,서활,Park, Jong-Chul,Kim, Kyung-Tae,Suh, Hwal 대한의용생체공학회 1997 의공학회지 Vol.18 No.3
본 시험조사는 정형외과, 신경외과, 재활의학과 등에서 경부 척추를 물리적으로 고정하는 데 널리 사용되고 있는 연성, 토마스, 미네르바 및 필라델피아 경추보조기의 물성을 조사하여 새로운 경추보조기를 고안하기위한 기초자료를 얻기 위하여 이루어 졌다. 인장강도는 토마스 보조기가 가장 우수했으며 지속적인 마모를 통한 재료 관통시험에서는 미네르바 보조기가 가장 우수한 내구력을 가진 것으로 나타났다. 이러한 결과로 미루어 볼 때, 에틸렌수지를 구조체로 사용하고 피부에 통기성을 부여하기 위해 재료에 다공성을 제공하는 것이 좋을 것으로 사료된다. This is to investigate the physical properties of the commercially available Soft, Thomas, Minerva, and Philadelphia cervical braces which are widely used in orthopedics, neurosurgery, and rehabilitation medicine clinics as assisting devices for physical stabilization of cervical vertebrates, to use as a basic data for designing new type brace. Tensile strengths were observed by universal mechanical measuring device and Thomas brace required the highest stress to break by tensile stress. Durabilities against continuous frictional forces were also determined, and Minerva brace demonstrated the longest frictional time until being perforated. According to these results, poly ethlene is recommendable as a frame and preparation of pores in the material is favorable to provide ventilation to skin.
유기질과 무기질 복합체를 이용한 체내흡수형 인공골재료에 관한 연구 -세포배양에 의한 생체적합성 평가-
안수진,김요숙,이춘기,서활,Ahn, Sue-Jin,Kim, Yo-Sook,Lee, Choon-Ki,Suh, Hwal 대한의용생체공학회 1995 의공학회지 Vol.16 No.3
체내 매입후 경시적으로 분해되면서 재생골조직에 의해 치환되는 인공골복합체를 제조하고 복합체가 세포활성에 미치는 영향을 조사하였다. 복합체시편을 세포배양액에 넣고 1주일동안 $37^{\circ}C$에서 배양한 다음, 사람자궁경부암유래 HeLa S3세포와 쥐피하 L929세포를 복합체가 용해된 세포배양액에서 5일간 배양하여 세포성장율을 비교하여 세포특성을 조사하였다. 한편 HeLa S3세포를 배양중인 배양액에 ${Na_2}^{51}CrO_4$를 첨가하여 HeLa S3세포에 $^{51}Cr$를 표식한 다음, 용해된 $^{51}Cr$의 양을 $\gamma$-counter를 이용하여 측정하였다. 세포성장정도의 측정에서는 HeLa S3 세포 및 L929 세포 모두가 특이한 세포독성을 발견할 수 없었으며, 복합체가 용해된 세포배양액내의 표식된 HeLa S3 세포로 부터 용해된 $^{51}Cr$량을 측정한 결과, 세포활성을 저해하지 않은 것으로 관찰되었다. A composite material was produced as an artificial bone substitute which is gradually degrAded and replaced by the regenerated natural bones after implantation. To detect the effect of the material on the cell's activity, the composite specimens were placed in MEMs and incubated at $37^{\circ}C$ for one week. Human uterus cervical cancer origin HeLa 3 cells and mouse subcutaneous origin L929 cells were cul- tured in the specimen dissolved MEMs for 5 days to investigate cytotoxicity via cell growth rates. ${Na_2}^{51}CrO_4$ solution was added to the media, to label the HeLa 53 cells, and the released amount of $^{51}Cr$ was measured by a $\gamma$-counter. On the cell growth investigation, no significant cytotoxic phenomena were revealed in both HeLa S3 and L929 cell cultures. On the released 51CR from the incubated HeLa 53 cells, no significant cell degeneration was observed from the composite embedded MEMs.
신정우 ( Jung U Shin ),정진영 ( Jin Young Jung ),서활 ( Hwal Suh ),정기양 ( Kee Yang Chung ) 大韓皮膚科學會 2009 大韓皮膚科學會誌 Vol.47 No.7
A giant cell tumor of tendon sheath is a slow growing benign soft tissue tumor that is known by a variety of names including fibrous histiocytoma of tendon sheath and fibrous xanthoma of the synovium. Clinically, it presents as a 1~3 cm firm, non-mobile, painless, nontender mass, and mostly occurs at interphalangeal joints of fingers. It shows female predominance and can occur at any age, but it is most common between the third and fifth decades and is rare in children. We now report the case of a 10-year-old girl with a giant cell tumor of tendon sheath on the toe. (Korean J Dermatol 2009;47(7):871~873)
신정우 ( Jung U Shin ),최윤진 ( Yoon Jin Choi ),노미령 ( Mi Ryung Roh ),정기양 ( Kee Yang Chung ),서활 ( Hwal Suh ) 대한피부과학회 2009 대한피부과학회지 Vol.47 No.7
Diabetic foot ulcer is a serious clinical problem with significant medical and economic effects on health systems worldwide. Some patients undergo amputation and others experience disability for an extended period of time. Treatment of diabetic foot ulcer is complex and difficult. Even with proper management, the wounds may not heal as well as expected. To promote wound healing, many advanced topical dressing materials have been developed. Among them, bi-layered collagen membrane, which is composed of collagen and hyaluronic acid, is believed to enhance wound healing. Herein we report two cases of diabetic foot ulcer which were successfully treated using bi-layered collagen membranes. (Korean J Dermatol 2009;47(7):831~834)