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Ferritin 단백질 내 나노입자 합성 및 전자현미경 연구
서향임 ( Hyang Yim Seo ),김성원 ( Sung Won Kim ),박영석 ( Young Seog Park ),김경숙 ( Kyung Suk Kim ) 조선대학교 공학기술연구원 2008 공학기술논문지 Vol.1 No.2
One of the important issues in the synthesis of nanoparticles was to how to regulate the particle size. Ferritin, an iron storage protein, has received of global attention in candidates for obtaining uniform particle size. Here we report our studies on synthesis of several nanoparticle with Al(3+), UO2(2+), and Fe(2+) using ferritin and characteristic of core mineral using TEM (transmission electron microscopy) and EDXA (Energy dispersive X-ray analysis). The reaction factors such as ionic strength, reaction volumes and protein concentrations were changed reconstitution yields of ferritin and size distribution of particle. On the basis of above-mentioned results, ferribydrite minerals within ferritin core were successfully induced using up to 3000 iron atoms per protein molecule. The crystallinity of mineral was directly confirmed using FE-TEM (field emission-energy filtering-transmission electron microscopy) and it was controlled by protein concentrations and iron amounts.