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        Enterococcus faecium CJNU 2008 균주 생산 박테리오신의 특성 규명

        서숙진,양정모,문기성 한국식품위생안전성학회 2018 한국식품위생안전성학회지 Vol.33 No.6

        Bacteriocin is a proteinaceous compound produced by microorganisms showing antimicrobial activities. In this study, the physicochemical properties of the bacteriocin produced by Enterococcus faecium CJNU 2008 strain were characterized. Partially purified bacteriocin showed stabilities against heat treatments at 100℃ for 30 min and 121℃ for 15 min and against solvents treatments such as methanol, ethanol, acetone, acetonitrile and chloroform. The bacteriocin also exhibited stabilities against lipase and α-amylase treatments but the stability was abolished at protease treatment, indicating that the antimicrobial agent from E. faecium CJNU 2008 was a proteinaceous bacteriocin. The bacteriocin also showed bactericidal mode of action against Listeria monocytogenes. The Molecular mass of the bacteriocin was estimated to be under 6.5 kDa by a tricine-SDS-PAGE analysis. The bacteriocin was purified by HPLC. Further studies toward biochemical analysis of the bacteriocin are needed in near future.

      • KCI등재후보

        케피어 유래 박테리오신 생산 Enterococcus faecium 균주 선발

        서숙진,문기성 한국유산균·프로바이오틱스학회 2016 Current Topic in Lactic Acid Bacteria and Probioti Vol.4 No.2

        A bacteriocin producing lactic acid bacterium was isolated from kefir which is functional fermented milk. The CJNU 2008 strain was identified as Enterococcus faecium by 16S rRNA gene sequence analysis and the strain named E. faecium CJNU 2008. Antibacterial spectrum of E. faecium CJNU 2008 has shown that several strains including pathogenic bacteria Listeria monocytogenes and Staphylococcus aureus were inhibited by the cell and culture supernatant. Optimal culture conditions for production of the bacteriocin from E. faecium CJNU 2008 were MRS broth as a medium, 30℃, and pH 7.0.

      • KCI등재후보

        Concurrent Surgery of Craniectomy and Splenectomy as Initial Treatment in Severe Traumatic Head Injury: A Case Report

        이형래,유남규,서숙진,최미선 대한신경손상학회 2017 Korean Journal of Neurotrauma Vol.13 No.2

        It is not a common case for neurosurgery department and the other departments to perform joint operation at the sametime. Patients with severe head injury are a condition in which vital signs are unstable due to severe brain swelling and increased intracranial pressure, and emergency surgery is required. A 44-year-old man visited the trauma center with a motorcycle accident. The Glasgow Coma Scale score at the time of emergency department was 3 points, and the pupil wasfxed at 6 mm on both sides. His medical history was unknown. His vital signs including blood pressure (BP), heart rate,respiratory rate and oxygen saturation were stable. Associated injuries included multiple fractures of whole body. Braincomputed tomography revealed subarachnoid hemorrhage, intraventricular hemorrhage and severe cerebral edema. During the preparation of the craniectomy, abdominal ultrasonography performed because of decreased BP resulted in a largeamount of hemoperitoneum. The bi-coronal craniectomy and splenectomy were performed simultaneously for about 4hours. After ffty days of treatment, he was discharged with Glasgow Outcome Scale-extended 4 points and is undergoingrehabilitation. In severe polytrauma patients, active concurrent surgery is a good method to save their lives.

      • KCI등재후보

        Concurrent Surgery of Craniectomy and Splenectomy as Initial Treatment in Severe Traumatic Head Injury: A Case Report

        이형래,유남규,서숙진,최미선 대한신경손상학회 2017 Korean Journal of Neurotrauma Vol.14 No.2

        It is not a common case for neurosurgery department and the other departments to perform joint operation at the sametime. Patients with severe head injury are a condition in which vital signs are unstable due to severe brain swelling and increased intracranial pressure, and emergency surgery is required. A 44-year-old man visited the trauma center with a motorcycle accident. The Glasgow Coma Scale score at the time of emergency department was 3 points, and the pupil wasfxed at 6 mm on both sides. His medical history was unknown. His vital signs including blood pressure (BP), heart rate,respiratory rate and oxygen saturation were stable. Associated injuries included multiple fractures of whole body. Braincomputed tomography revealed subarachnoid hemorrhage, intraventricular hemorrhage and severe cerebral edema. During the preparation of the craniectomy, abdominal ultrasonography performed because of decreased BP resulted in a largeamount of hemoperitoneum. The bi-coronal craniectomy and splenectomy were performed simultaneously for about 4hours. After ffty days of treatment, he was discharged with Glasgow Outcome Scale-extended 4 points and is undergoingrehabilitation. In severe polytrauma patients, active concurrent surgery is a good method to save their lives.

      • KCI등재후보

        Continuous Renal Replacement Therapy for Acute Renal Failure in Patients with Traumatic Brain Injury

        박장용,최현용,유남규,노태훈,서숙진,김세혁 대한신경손상학회 2016 Korean Journal of Neurotrauma Vol.12 No.2

        Objective: The purpose of this study was to investigate the impact of continuous renal replacement therapy (CRRT) on survival and relevant factors in patients who underwent CRRT after traumatic brain injury (TBI). Methods: We retrospectively reviewed the laboratory, clinical, and radiological data of 29 patients who underwent CRRT among 1,190 TBI patients treated at our institution between April 2011 and June 2015. There were 20 men and 9 women, and the mean age was 60.2 years. The mean initial Glasgow Coma Scale score was 9.2, and the mean injury severity score was 24. Kaplan-Meier method and Cox regression were used for analysis of survival and relevant factors. Results: The actuarial median survival time of the 29 patients was 163 days (range, 3-317). Among the above 29 patients, 22 died with a median survival time of 8 days (range, 3-55). The causes of death were TBI-related in 8, sepsis due to pneumonia or acute respiratory distress syndrome (ARDS) in 4, and multi-organ failure in 10. Among the various factors, urine quantity of more than 500 mL for 24-hours before receiving CRRT was a significant and favorable factor for survival in the multivariate analysis (p=0.026). Conclusion: According to our results, we suggest that early intervention with CRRT may be beneficial in the treatment of TBI patients with impending acute renal failure (ARF). To define the therapeutic advantages of early CRRT in the TBI patients with ARF, a well-designed and controlled study with more cases is required.

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