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Bacillus insolitus ATCC 23299 로부터 유래된 Promoter 의 클로닝과 발현
박정현,박승국,장정수,이상화,변시명 ( Jung Hyen Park,Seung Kook Park,Jeong Su Jang,Sang Hwa Lee,Si Myung Byun ) 생화학분자생물학회 1992 BMB Reports Vol.25 No.5
In order to investigate potential promoter activites originated from psychrophilic Bacilli, we tried promoter cloning in Bacillus subtilis DB104 by using our characteristic promoter probe shuttle vector, pZC101 which was constructed by ligation of promoterless chloramphenicol acetyltransferase-86 (cat-86) gene and plasmid pZ124. Four already-known promoters (lac, tac, aprE and spoII) have been subcloned into the promoter cloning sites of pZC101 for the purpose of using standards to compare promoter strength on cloned promoters. We cloned 55 DNA fragments with promoter activities in B. subtilis DB104 from two kinds of Bacillus chromosomal DNA source, Bacillus insolitus ATCC23299 (psychrophile) and Bacillus subtilis JU70 (mesophile). Judging from specific activity on CAT, pZC11 promoter derived from B. insolitus ATCC23299, has the highest transcription promoting activity in this study where pZC11 promoter expressed about 13 U of CAT which was 3 fold higher CAT specific activity than spoII promoter in B. subtilis DB104.