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서양종 꿀벌(Apis mellifera)과 동양종 꿀벌(Apis cerana)에서 Nosema ceranae (Microsporidia)의 진단과 비교
변규호(Kyu Ho Byeon),트란 반 또안(Tran Van Toan),이명렬(Myeong Lyeol Lee),심하식(Ha Sik Sim),김혜경(Hye Kyung Kim),윤미영(Mi Young Yoon),홍인표(In Pyo Hong),우순옥(Soon Ok Woo),최용수(Yong-Soo Choi) 한국양봉학회 2014 韓國養蜂學會誌 Vol.29 No.4
The worldwide beekeeping countries has been lose colonies increasingly. Nosema spp. is one of factors about colony lose. Nosema spp. are a parasite of not only the European honey bee, Apis mellifera, but also several Apis spp. (Apis cerana etc.); However, little is known about the effects of N. ceranae on A. mellifera and A. cerana. Nosema positive samples (determined from light microscopy of spores) of adult worker bees were tested to determine Nosema species using species specific PCR primers of the 16S rDNA gene for detection of N. Apis and N. ceranae. Additionally we were detected of Nosema spp. by normal primer, it was detection of both Nosema spp. PCR detection methods is easier than morphologically method to distinguish N. ceranae and N. apis. We were detected only N. ceranae in all samples, indicating that this species present in apiaries by gene specific PCR. While our research was for one year, it can determinants about seasonal infection that there may be a seasonality to N. ceranae infections. Nosema spp. were increased at the spring season; So, we were counted number of spores of Nosema spp. in A. mellifera and A. cerana colonies at spring season by haemocytometer. Being able to foresee future Nosema spp. infections can be used to inform beekeepers on Nosema spp. management.
울금 추출물 처리에 의한 꿀벌 노제마 포자 증식 억제 효과
김혜경(Hye-Kyung Kim),변규호(Kyu Ho Byeon),강은진(En Jin Kang),이명렬(Myeong-Lyeol Lee),이경용(Kyeong Yong Lee),최용수(Yong-Soo Choi) 한국양봉학회 2016 韓國養蜂學會誌 Vol.31 No.4
Nosema disease in honeybees is caused by the microsporidian parasites Nosema apis and Nosema ceranae. Between the two, N. ceranae has been reported as an important threat to honey bee health. Our objective was to evaluate the effectiveness of ulgeum (Curcuma longa L.) extract for the control of N. ceranae in honeybees. In our study, ulgeum was extracted with methanol. A worker bee infected with N. ceranae spore was dosed and fed with ulgeum extracts at different concentrations (0%, 0.1%, 0.25%, 0.5%, and 1%). The number of spores in 20 days significantly decreased when 0.5% ulgeum extract was administered. Moreover, the ulgeum extract was not toxic to bees at less than 1%. The data suggest that turmeric could be useful in alternative strategies for the control of N. ceranae.
꿀벌의 신규 침입 해충, 작은벌집딱정벌레(Aethina tumida Murray, 1867)에 대한 방제 약제 개발과 현장 적용 평가
김동원(Dongwon Kim),이명렬(Myeong-lyeol Lee),이만영(Man-Young Lee),최용수(Yong-Soo Choi),김혜경(Hye-Kyung Kim),변규호(Kyu-ho Byeon),김성희(Seong-Hee Kim) 한국양봉학회 2018 韓國養蜂學會誌 Vol.33 No.3
Small hive beetle (SHB, Aethina tumida Murray (Coleoptera: Nitidulidae) is a honeybee pest infesting combs and stores inside the hive. Contamination of the SHB on Apis mellifera colonies were firstly noticed on September 23, 2016, in Miryang City, Gyeongnam Province in Korea. We tested the preference rate of bait against small hive beetle larvae and adult. When treated each bait of preference showed highest pollen or pollen added sugar water. We was selected the coumaphos through insecticide screening. LC/LD<SUB>50</SUB> values of coumaphos on small hive beetle larvae was estimated as 612ppm, and 1.591ug/beetle and fluvalinate 4,641ppm and 12.067ug/beetle. LC<SUB>50</SUB> values of coumaphos on small hive beetle adult was estimated as 6,571ppm. we suggested pollen patty mixed with coumaphos 10,000ppm as a control agent against small hive beetle. The control agent was tested with CD type trap and showed 82% control efficiency after 15 days in field. This study should be used a fundamental information for developing IPM (Integrated Pest Management) and controlling the expansion of population of small hive beetle in Korea.
꿀벌에 기생하는 꿀벌응애(Varroa destructor)와 중국가시응애(Tropilaelaps clareae)의 형태적 특성과 생태적 상호작용
최용수(Yong-Soo Choi),이명렬(Myeong Lyeol Lee),심하식(Ha Sik Sim),김혜경(Hye Kyung Kim),변규호(Kyu Ho Byeon),윤미영(Mi Young Yoon),강아랑(Ah Rang Kang),트란 반 또안(Tran Van Toan),홍인표(In Pyo Hong),우순옥(Soon Ok Woo) 한국양봉학회 2014 韓國養蜂學會誌 Vol.29 No.4
The ectoparasitic honey bee mites was originally confined to the Asian honey bee (Apis cerana etc.). Mites attach to adults and developing brood, where they feed on the hemolymph. If left untreated, mites can deform bees and eventually kill the colony. Varroa destructor and Tropilaelaps clareae have plagued European honey bees, Apis mellifera. Differences in mite tolerance are reported between two honey bee species A. mellifera and A. cerana. We counted number of mites (V. destructor and T. clareae) from 20 colonies. V. destructor and T. clareae has very similar behavior in honeybee colony. When behaviorally close, two competing species may populational interfere, and thereby affect their population dynamics. We tested for populational interference (PI) between two populational competing honeybee mites, V. destructor and T. clareae, by investigating their population dynamics when they competed on the same colony. Population of V. destructors are increased during August, whereas population of T. clareaes are decreased. The effects of interspecific competition on the population growth of mites (focus in the colony) are summarized in data. The presence of T. clareae on with V. destructor led to reduce in the change of T. clareae population size at August compared with other season, indicating that some form of interspecific competition occurred between the two species.
도토리화분과 다래화분의 일반성분, 지방산 분석 및 형태 관찰
홍인표 ( In Pyo Hong ),이만영 ( Man Young Lee ),우순옥 ( Soon Ok Woo ),심하식 ( Ha Sik Sim ),최용수 ( Yong Soo Choi ),한상미 ( Sang Mi Han ),김혜경 ( Hye Kyung Kim ),변규호 ( Kyu Ho Byeon ),이명렬 ( Myeong Lyeol Lee ),김정봉 ( Jun 한국잠사학회 2013 한국잠사곤충학회지 Vol.51 No.2
Pollens have been known to possess various biological properties. Therefore, pollens have been extensively used in functional food, folk medicine, and beverage industry to improve human health. This study was conducted to establish the optimized protocol for cytoplasm isolation of bee pollen. Data of biochemical parameters and fatty acid profiles were obtained from pollens of Acorn and Darae (Actinidia arguta). Contents of crude protein and crude fat were 24.1% and 11.8% in Acorn pollen, and those of Darae pollen showed 35.8% and 8.7% in crude protein and crude fat respectively. Also after lyophilizing of Acorn pollen, content of crude protein was increased to 26.5%. Main fatty acids were palmitic acid (C16 : 0), oleic acid (C18 : 1), linoleic acid (C18 : 2) and linolenic acid (C18 : 3) in bee pollen. Linoleic acid (37.3%) was dominant fatty acid in Acorn pollen that is one of essential fatty acids. Linolenic acid (48.3%) was dominant fatty acid in Darae pollen that is a polyunsaturated fatty acid. The proportion of unsaturated fatty acids to total acid content was 73.2% in Acorn and 63.2% in Darae pollen, and especially that of polyunsaturated fatty acids was higher than 55%.
김혜경(Hye-Kyung Kim),홍인표(In-Pyo Hong),트란 반 또안(Tran Van Toan),이명렬(Myeong-Lyeol Lee),최용수(Yong-Soo Choi),심하식(Ha-Sik Sim),변규호(Kyu Ho Byeon),강아랑(Ah Rang Kang) 한국양봉학회 2015 韓國養蜂學會誌 Vol.30 No.2
Nosemosis (Nosemosis apium) is one of the most important parasites of honey bee, a recently described pathogen of Apis mellifera and Apis cerana. In honey bees, nosemosis infections are caused by microsporidian species of Nosema apis and Nosema ceranae. Nosemosis symptoms include digestive and absorption disorders because the spores damage epithelial tissue of the alimentary canal that is responsible for food absorption. Although fumagillin is the only antibiotic approved for control of Nosema disease in honey bees, fumagillin is known to be toxic to mammals and has a risk of residues in honey. In this paper, we have been conducting experiments of reduce production of Nosema spores using newly emerging bees that orally inoculated with Nosema ceranae spores. The data shown that the medium of Cordyceps militaris reduced spore production of Nosema cerenae to 97.82% compared with control and can be used for eco-friendly material to control of Nosema cerenae.
뒤영벌류(Bombus terrestris와 Bombus ignitus)에서 꿀벌바이러스 진단
최용수(Yong Soo Choi),이만영(Man Young Lee),홍인표(In Pyo Hong),김남숙(Nam Suk Kim),김혜경(Hye Kyung Kim),변규호(Kyu Ho Byeon),윤형주(Hyung Joo Yoon) 한국양봉학회 2010 韓國養蜂學會誌 Vol.25 No.4
Mortality of honeybees is a serious problem that beekeepers have to face periodically in Korea and worldwide. The presence of RNA viruses, in addition to other pathogens may be one of its possible causes. A survey on the occurrence of seven honeybee-pathogenic viruses was carried out using RTPCR assays. In this work, we detected Deformed Wing Virus (DWV), Israle Acute Paralysis Virus (IAPV), Black Queen Cell Virus (BQCV), Cloudy Wing Virus (CWV), Kashmir Bee Virus (KBV), Sacbrood Virus (SBV), Chronic Bee Paralysis Virus (CBPV) in samples of Bumblebee (Bombus terrestris L. and Bombus ignitus). The detection of viruses in all Bumblebee samples, simultaneous co-infection of colonies by several viruses, indicates they are widely spread in the colony. Honeybee virus is closely associated with characteristic wing deformities, abdominal bloating, paralysis, and rapid mortality of emerging adult and larval stage bees. Here, we report for the first time the occurrence of multi-virus infected bumble bees (B. terrestris and B. ignitus). For the present study, the detection of bee virus from the adult bumble bee, death adult bumblebee, mail bumble bee, pupa stage bumblebee and pollen to the infection cycle was investigated in the same colony. Using specific RT-PCR protocols for the detection of virus followed by sequencing of the PCR products we could demonstrate that the bumblebees were indeed infected with several bee viruses.
동양종 꿀벌 항균단백질(Royalisin and Hymenopteacin)의 유전자 구조 분석
최용수(Yong Soo Choi),이만영(Man Young Lee),홍인표(In Pyo Hong),김남숙(Nam Suk Kim),김혜경(Hye Kyung Kim),변규호(Kyu Ho Byeon),이명렬(Myeong Lyeol Lee),이광길(Kwang Gill Lee) 한국양봉학회 2010 韓國養蜂學會誌 Vol.25 No.1
The Asiatic honeybee, Apis cerana Fabricius, is an important honeybee species in Asian countries. It is still found in the wild, but is also one of the few bee species that can be domesticated. It has acquired some genetic advantages and significantly different biological characteristics compared with other Apis species. However, it has been less studied. We designed primers for the sequences of the antimicrobial peptide cDNA genes (royalisin and hymenoptaecin) of the Asiatic honeybee, A. cerana. All the sequences were amplified by reverse transcriptase-polymerase chain reaction (RT-PCR). In all, antimicrobial peptides (royalisin and hymenoptaecin) were cloned and identified from the Asiatic honeybee, A. cerana adult workers. Royalisin is an antibacterial peptide found in Royal Jelly. The royalisin gene fragments of Asiatic honeybee (Apis cerana), 285bp cDNA encoding pre-pro-Acroyalisin (Ac-Royal) of 95 amino acid residues were cloned into the pGEM-T vector and than sequence analysed. Hymenoptaecin is one of important antibacterial peptides in honey bee. Both A. cerana and A. mellifera (GenBank No. AAR01214) royalisins consist of 95 amino acids. The hymenoptaecin gene fragments of Asiatic honeybee (A. cerana), 378bp cDNA encoding pre-pro-Ac-hymenoptaecin (Ac-Hym) of 126 amino acid residues were cloned into the pGEM-T vector and than sequence analysed. Compared to A. mellifera hymenoptaecin (GenBank No. AAA67444), which is a cationic polypeptide with 126 amino acids, mature A. cerana hymenoptaecin is 129 amino acids long, suggesting structural and functional similarity to A. mellifera hymenoptaecin.
김혜경(Hye-Kyung Kim),이명렬(Myeong-Lyeol Lee),이만영(Man-Young Lee),최용수(Yong-Soo Choi),김남숙(Nam-Suk Kim),홍인표(In-Pyo Hong),변규호(Kyu-Ho Byeon),이광길(Kwang Gill Lee),진병래(Byung Rae Jin) 한국양봉학회 2009 韓國養蜂學會誌 Vol.24 No.2
This study aimed to evaluate the antioxidant activity of chestnut honey which were harvested at various areas in South Korea. First at all, we measured the total phenols content through a spectrophotometric determination with a modified Folin-Ciocalteu method and total flavonoids content determined with aluminium chloride. Total phenolic compounds was highest in Sunchang of Chestnut honey (2.21㎎/㎖) and flavonoids contents was also the highest in Sunchang of Chestnut honey (1.02㎎/㎖) than other samples. For measured the antioxidant activity of chestnut honey, we performed DPPH (2,2-diphenyl-1-picrylhydrazyl) test and FRAP (ferric reducing-antioxidant assay) test. DPPH scavenging activity highest in Sunchang of Chestnut honey more than 50% DPPH scavenging activity while other samples (Gong-ju, Yechen, Chung-ju, Imsil, Ha-dong) showed more than 25% DPPH scavenging activity. The ferric reducingantioxidant assay (FRAP) is based on the reduction of ferric 2,4,6-tris(2-pyridyl)-1,3,5-triazine [Fe(Ⅲ)-TPTZ] by spectrophotometric analysis. Sunchang were found to have more than 532μM FRAP activity while other samples (Gong-ju, Yechen, Chung-ju, Imsil, Ha-dong) showed more than 300μM FRAP activity.
호박벌( Bombus ignitus)에서의 Deformed Wing Virus (DWV) 진단
최용수(Yong Soo Choi),이만영(Man Young Lee),이명렬(Myeong Lyeol Lee),김혜경(Hye Kyung Kim),김남숙(Nam Suk Kim),홍인표(In Pyo Hong),변규호(Kyu Ho Byeon),이광길(Kwang Gill Lee),윤형주(Hyung Joo Yoon) 한국양봉학회 2009 韓國養蜂學會誌 Vol.24 No.2
We report for the first time the occurrence of DWV-infected bumble bees (Bombus ignitus). For the present study, the detection of DWV virus from the female and male bumble bee was investigated in the same colony. The Deformed wing virus (DWV) of honeybee (Apis mellifera) is closely associated with characteristic wing deformities, abdominal bloating, paralysis, and rapid mortality of emerging adult bees. Using specific RT-PCR protocols for the detection of DWV followed by sequencing of the PCR products we could demonstrate that the bumble bees were indeed infected with DWV. The virus was detected from Bombus ignitus, and its partial DWV gene was cloned and sequenced. The partial DWV gene encoding the polyprotein is 711-nt of 235 amino acid residues. The deduced nucleotide sequence of the polyprotein partial gene of DWV showed 96.9%, 96.2%, 96.8%, and 96.5% homology to other structure polyprotein partial gene of DWV from insects, respectively. Phylogenetic analysis further conformed that the deduced nucleotide sequence of the polyprotein partial gene of DWV divided to the outside tree. We describe the first time that presence of Deformed wing virus (DWV) from bumble bee (Bombus terrestris) in Korea using RT-PCR.