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베드리칸난,Natarajan Sakthivel,한성수 한국섬유공학회 2021 Fibers and polymers Vol.22 No.4
Metal- and carbon-based nanoparticles (MBNPs/CBNPs) with different nanometric dimensions have unique combinations of physical and chemical properties. The physico-chemical properties, morphology, and interactions of nanomaterials with biological systems differ according to the source of synthesis and the extent and type of functionalization involved, which have many advantages for exciting nanotechnological applications in the fields of chemical and biological sciences. The biosynthesis and biofabrication of nanomaterials are achieved in a myriad of ways using plants and microorganisms. Greener synthesis has been conducted using the extracts of plants or other biological entities. This review focuses on the recent insights in the green and biological synthesis of MBNPs based on cadmium, cerium, copper, gold, iron, manganese, nickel, palladium, platinum, selenium, silver, titanium, yttrium, zinc, and zirconium, and CBNPs, such as carbon dots (CDs), as well as their chemical and biological applications, such as chemical catalysis, anticancer, antioxidant, antimicrobial, antibiofilm, antidiabetic, insecticidal, scolicidal, antileshmanial, angiogenesis, and immunostimulant agents, and as tools for bioimaging, biosensing, and drug delivery.
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베드리칸난,원소연,K. Narayanan Rajnish,한성수 한국공업화학회 2021 Journal of Industrial and Engineering Chemistry Vol.101 No.-
Graphene-based nanomaterials are rapidly emerging as antimicrobial agents against a range of pathogenicmicroorganisms. In the present study, we have demonstrated the synthesis of reduced grapheneoxide (RGO) using Aspergillus sp. filtrate as a reducing agent. The obtained Aspergillus sp. filtrate-RGO(AF-RGO) was characterized by ultraviolet–visible absorption spectroscopy, dynamic light scattering,X-ray diffraction, Fourier-transform infrared spectroscopy, Raman spectroscopy, scanning electronmicroscopy, transmission electron microscopy, X-ray photoelectron spectroscopy, and atomic force spectroscopyto obtain details about the size, composition, structure, and morphology. The characterizationconfirmed the reduction of graphene oxide, the restoration of sp2 conjugation, and the formation offew-layered RGO sheets. The surface charge and stability of the AF-RGO in aqueous media were examinedby zeta potential measurements. The thermal stability of AF-RGO was increased significantly due to therestoration of the graphitic sp2 network. The antibacterial activity of AF-RGO was evaluated using theagar well diffusion method, broth microdilution method, and Live/Dead bacterial viability assay. AFRGOexhibited good antibacterial activities against pathogenic Gram-negative (Escherichia coli) andGram-positive (Staphylococcus aureus) bacteria with minimum inhibitory concentration (MIC) and minimumbactericidal concentration (MBC) values of 15 and 30 mg/ml, respectively. Therefore, fungal filtratemediatedsynthesis of RGO is a promising antibacterial carbon nanomaterial for a range of biomedical andnano(bio)technological applications.
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베드리칸난,박현호 한국화학공학회 2015 Korean Journal of Chemical Engineering Vol.32 No.7
A new greener strategy for the synthesis and stabilization of gold nanoparticles using aqueous turnip leaf extract under ambient conditions is reported in this study. The formation of gold nanoparticles was monitored using a UV-Vis spectrophotometer and the maximum absorption peak (λmax) at 535 nm with a visual color change to pinkishred confirmed the gold nanoparticles. Further characterization was conducted using Fourier-transform Infra-red spectrometry (FT-IR), powder X-ray diffractometry (XRD), transmission electron microscopy (TEM), and dynamic light scattering (DLS) with zeta potential at pH 7.5. The stability of the nanoparticles was due to the capping of nanoparticles with amine groups and ortho-substituted aromatic phytoconstituents, which exhibit higher negative values of zeta potential (ζ). XRD pattern revealed the formation of face-centered cubic (fcc) lattice crystals of gold nanoparticles, while TEM have demonstrated the size of gold nanoparticles ranging from 3 to 58 nm. The as-synthesized gold nanoparticles showed rapid catalytic reduction of methylene blue (MB) dye to leuco MB in the presence of sodium borohydride (NaBH4). The reduction reaction followed pseudo-first order kinetics with a reaction rate constant of 0.372 min−1. This process of nanoparticle synthesis is simple, nontoxic and environmentally benign compared to the chemical synthetic routes.
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Prakash Shrestha,Shiva Pathak,Shobha Regmi,베드리칸난,Tiep Tien Nguyen,Simmyung Yook,김종오,Jee-Heon Jeong 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.115 No.-
Extracellular matrix (ECM) is an integral part of cell biology that not only provides physical stability tocells, but also aids in cell-to-cell and cell-to-essential-protein interactions in the cellular microenvironment. To mimic the natural ECM conditions, we prepared morphologically similar artificial polymericmicrofibers and used polydopamine to coat them with collagen to enhance cell–polymeric fiber interactions. Heterospheroids (HSs) of ECM-mimicking microfibers and mesenchymal stem cells (MSCs) wereprepared using the spontaneous aggregation method. The results revealed well-organized MSCmicrofiberHSs that enhanced the viability of the MSCs. In addition, when curcumin, an antioxidant drug,was loaded into the microfibers and HSs were prepared, the viability of the MSCs was further enhanced,as revealed by a reduction in the expression of apoptotic protein Bax. Furthermore, the expression of antiapoptoticproteins Bcl-2 and HO-1 increased significantly in the HSs containing the drug-loaded microfibers. Overall, our study suggests a novel strategy for improving the success of MSC therapy using ECMmimickingartificial microfibers.
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