간호실무를 통하여 인지한 간호개념에 대한 조사연구

백혜자 中央醫學社 1990 中央醫學 Vol.55 No.1

The study is aimed at exploring recognized nursing concept through nursing business in the spot of nursing care. The data were collected from 118 nurses conveniently selected from the population of nurses working in Kang Won proving, from July 20 to August 20,1989. for one month. The main result of this study can be summarized as follows; 1. In general characteristic the age distribution of examined respondents is the largest in number from 20 year old to 34 year old (79.7 %). The ratio of educational level was 91.5 % in nursing college graduation. The job sites were 32.2 % of respondents in hospital, 43.2 % of them in public health center and medical office, nurse teacher (18.6 %), industrial sites (5.9 %) respectively. Nurses with over five-year career were 53 %. Religion showed 37.3 % of Protestant, 14.4 of Catholic and 13.6 % of Buddhism. Marital status showed 48.3 % in marriage and 50.9 in single. 2. Nurse teachers (59.1 %) and nurses working in public health center and medical office (45.1 %) relatively highly recognized nursing service as technical profession with originality. Nurses working in hospital (39.5 %) and industrial sites (14.2 %) recognized at a low degree. In general most respondents (44.1 %) regarded nursing job as automomous technical profession. A difference of view in each nursing field didn't pay attention (p < 0.05). 3. Nursing laid an emphasis on the sot of nursing care was carried out at hospital (100 %), public health center and medical office (90.2 %), nurse teacher (86.47o), and all mentioned were executed in physical side. In every domain nursing execution on social (48.3 %) and spiritual side (33.9 %) was neglected. 4. According to responded survey deficient quality as nurse was technical knowledge -77-in hospital (57.9 %), industrial sites (57.1 %), and was sincerity and responsibility.(35.2 %) in public health center and medical office, and was satisfaction (27.2%) and technical knowledge (27.2 %) concerning nurse teacher. Besides, response about deficient quality were different in each field (p < 0.05). 5. On clients of nursing care, respondents answered that in hospital nursing care was carried out at the lowest degree for family (39.5,7o), group (23.7 %). Nursing care to nurses themselves (54.9 %), group (75.6 %) was executed at a relatively low degree in public health center and medical office. In school nursing care was performed at a lower degree for guardian (40.9 %) group or nurses themselves (14.2 %), too. 6. As regards nursing activity with important aim in the spot of nursing care, the high degree of nursing activities consisted of cooperation of curing disease (89 %), health education (83.0 %) and decreasing of pain of clients. On the other hand low degree of nursing activities were disease prevention (69.5%), maintaining health (63.617o) and health promotion (62.7 %) 7. Among Maslow's human needs, the order showing the lowest interests during the nursing care can be summarized as follows; self-realization (72.0 %), esteem need (44.9 %), need for love (42.7 %); that is to say, nursing care for satisfying the high rank need has the lowest interest. 8. In the spot of nursing care, in order to satisfy nursing care needs of Virginia Henderson's basic needs this study was done to investigate the degree of nursing care execution. In every items the ratio of nursing care execution was low; in particular in execution of nursing care for satisfying the high rank needs such as Preparation for recreation time (2.29 ± 1.065), Through work that is suitable for health, help was given with a view to feeling achievement (2.364 ± 1.091) Help is given to clients for recreational activities (2.525 ± 1.167), Psychological knowledge for clothing is put into use in nursing care (2.661 ± 1.094). Besides, Nursing care for normal excretion (2.574 ± 1.408), and Airpollution Prevention(2.873 ± 2.561) were low, too.

점망둑 , Chasmichthys dolichognathus 의 산란습성과 발생

백혜자,김형배,이택열 한국수산과학회 1984 한국수산과학회지 Vol.17 No.6

N/A

점망둑 , Chasmichthys dolichognathus 의 생식기구에 관한 실험적 연구

백혜자,이택열 한국수산과학회 1984 한국수산과학회지 Vol.17 No.6

N/A

점망둑 , Chasmichthys dolichognathus 의 생성숙과 산란

백혜자,김형배,이택열 한국수산과학회 1984 한국수산과학회지 Vol.17 No.6

N/A

점망둑(Chasmichthys dolichognathus)의 성숙기 난모세포에서의 $C_{21}$-스테로이드 생성

백혜자,Baek, Hea-Ja 한국발생생물학회 2008 발생과 생식 Vol.12 No.1

To investigate the $C_{21}$-steroids produced from maturating oocytes in the longchin goby, Chasmichthys dolichognathus, the oocytes ($0.74{\sim}0.97\;mm$) were incubated with radiolabeled $17{\alpha}$-hydroxyprogesterone ($^3H-17{\alpha}OHP$) for 24 hours. The resulting metabolites were analyzed by thin layer chromatography and identified by gas chromatography-mass spectrometry. Two $C_{21}$-steroids, $17{\alpha}$-hydroxy, $20{\alpha}$-dihydroprogesterone ($17{\alpha}20{\alpha}P$) and $17{\alpha}$-hydroxy, $20{\beta}$-dihydroprogesterone ($17{\alpha}20{\beta}P$), were converted from $^3H-17{\alpha}OHP$ in the maturing oocytes. These two main metabolites were detected at 0.80 mm diameter oocytes or greater. In addition, the effects of these metabolites on in vitro germinal vesicle breakdown (GVBD) were tested. The sensitivity of oocytes to the induction of GVBD was greater at $17{\alpha}20{\beta}P$ than $17{\alpha}20{\alpha}P$. This result showed that $17{\alpha}20{\beta}P$ is a major maturation inducing steroid (MIS) in longchin goby, suggesting $17{\alpha}20{\alpha}P$ may play a role in regulating the oocyte maturation process.

무지개송어 (Oncorhvnchus mvkiss) 난소여 포의 협막층 (Theca layers)을 통한 스테로이드 대사 분석

백혜자,이택열 한국통합생물학회 1993 동물학회지 Vol.36 No.1

난모세포성숙기 (peripheral germinal vesicle stage)의 무지개송어(Oncorhynchus mykiss)를 대상으로 방사성 표지 전구체(3H-pregnenolone, 3H-l7$\alpha$-hydroxyprogesterone 그리고 3H-androstenedione)를 이용하여 유 nitro에서 관찰한 협막세포층의 스테로이드 주 대사 경로는 다음과 같으며, pregnenolone -) 17a-hydroxypregnenolone$\longrightarrow$ 17u-hydroxyprogesterone $\longrightarrow$ androstenedione $\longrightarrow$ testosterone 이는 $\Delta$5-스테로이드 경로의 존재가능성을 지적하고 있다.

Changes in Plasma Steroid Hormone Levels and Gonad Development by the Control of Photoperiods and Water Temperatures on Timing of Sexual Maturity of Rockfish (Sebastes schlegeli)

백혜자,김형배,노섬,이영돈,박무억 한국수산과학회 2004 Fisheries and Aquatic Sciences Vol.7 No.1

Plasma steroid hormone levels in the viviparous rockfish (Sebastes schlegeli) were examined in relation to gonadal histology under controlled photoperiods and water temperatures. To investigate those effects in S. schlegeli the photoperiod was maintained at 15L:9D in June and then it was gradually decreased to 9L:15D in October. It was then gradually increased to 12L:12D in January, followed by 14L:10D in February. The water temperature was 19-20℃ in July. From August to October, it was from 18℃ to 12℃. Then, it was dropped to a low of 9-11℃ in November to December and then gradually increased to 14-15℃ in February. In females, both plasma estradiol-17β (E2) and testosterone (T) levels from August to February showed a similar pattern in both the treatment and the control groups. In the treatment group, the peaks of plasma E2 and T were observed in November, and the peaks were closely correlated to histological observations. Oocytes contained many yolk globules (final vitellogenic oocytes), and oocytes at the migratory nucleus stage increased in size. Plasma levels of progesterone did not change much throughout the experimental period. However, in the control group, the peaks of E2, T, and progesterone were observed in February. These results indicate that the controlled photoperiod and water temperature accelerated sexual maturity, corresponding to the advancement of plasma E2 and T peaks by approximately 3 months. In males, plasma T levels showed a similar pattern from August to October in the treatment and control groups, though levels in the treatment group were higher than those in the control group. From histological observations, the treatment group copulated one month earlier.

Production of -Steroids in Longchin Goby, Chasmichthys dolichognathus during Oocyte Maturation

백혜자 한국발생생물학회 2008 발생과 생식 Vol.12 No.1

점망둑의 난모세포 성숙과정에서 생성되는 주요 성 스테로이드 호르몬, -스테로이드를 분석하고자 전구물질 ()를 성숙기 난모세포(난경 ) 배양초기에 첨가하여 24시간 배양하였다. 스테로이드 대사물질 분석과 동정은 thin layer chromatography와 gaschromatography-mass spectrometry로 이루어졌다. 로부터 생성된 주요 성 스테로이드 대사물질은 -hydroxy, -dihydroprogesterone ()와 -hydrox To investigate the -steroids produced from maturating oocytes in the longchin goby, Chasmichthys dolichognathus, the oocytes () were incubated with radiolabeled -hydroxyprogesterone () for 24 hours. The resulting metabolites were analyzed by thin layer chromatography and identified by gas chromatography-mass spectrometry. Two -steroids, -hydroxy, -dihydroprogesterone () and -hydroxy, -dihydroprogesterone (), were converted from in the maturing oocytes. These two main metabolites were detected at 0.80 mm diameter oocytes or greater. In addition, the effects of these metabolites on in vitro germinal vesicle breakdown (GVBD) were tested. The sensitivity of oocytes to the induction of GVBD was greater at than . This result showed that is a major maturation inducing steroid (MIS) in longchin goby, suggesting may play a role in regulating the oocyte maturation process.

범가자미, Verasper variegatus의 난황단백질 분리 및 면역학적 특성

백혜자,한창희,김우진,박중연,김진한,김윤 동의대학교 기초과학연구소 1998 基礎科學硏究論文集 Vol.8 No.1

The yolk protein of spotted flounder, Verasper variegatus was purified by precipitation with cold distilled water, followed by Sepharose CL-6B column chromatography. The purified protein was identified as vitellin by Ouchterlony's immunodiffusion test and immunoelectrophoresis. The purified vitellin from ovarian crude extracts has same antigenic determinants with the female specific serum protein, vitellogenin. The molecular weight of purified vitellin was estimated about 550kD by gel filtration. The vitellin was composed of three major subunits with molecular weight of about 108, 85 and 31 kD, and two minor subunits. The vitellin was identified by western blot analysis using anti-vitellin antibody.

In Vitro Steroidogenesis on Oocyte Development in the Starry Flounder, Platichthys stellatus

백혜자,김형배,Dea Geun Kim 한국발생생물학회 2013 발생과 생식 Vol.17 No.4

In this study, oocyte steroidogenesis are investigated in relation to oocyte development in the starry flounder, Platichthys stellatus, a marine multiple spawner. Vitellogenic (0.52 and 0.55 mm oocyte diameter) and mature oocytes (0.63, 0.66 and 0.71 mm oocyte diameter) were incubated in vitro in the presence of [3H]17α-hydroxyprogesterone ([3H]17α- OHP) as a precursor. Steroid metabolites were extracted from the incubated media and oocytes, the extracts were separated and identified by thin-layer chromatography (TLC), high performance liquid chromatography (HPLC) and gas chromatography- mass spectrometry (GC-MS). The major metabolites produced from [3H]17α-OHP were androgens [androstenedione (A4) and testosterone (T)] and estrogens [17β-estradiol (E2) and estrone (E1)] and progestins [17α,20α-dihydroxy-4-pregnen- 3-one (17α20αP) and 17α,20β-dihydroxy-4-pregnen-3-one (17α20βP)] in vitellogenic and mature oocytes. The results from this study suggest the potential roles of E1 in the oocytes with diameter 0.52-0.71 mm, 17α20αP and 17α20βP at the oocytes of 0.63, 0.66 and 0.71 mm.