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RISS 인기검색어
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- 저자 : 백종현(Jeong Hyon Baek) (검색결과 1 건)
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백종현(Jeong Hyon Baek),이증훈(Yeul Hoon Sung),성열훈(Jeung Hoon Lee),박장규(Jang Kyu Park),박정규(Jeong Kyu Park) 대한피부과학회 1990 大韓皮膚科學會誌 Vol.28 No.2
Since adult human skin can be grown in chernically defined medium without serum, the skin organ culture has gained a great interest as a method for studies concerning skin biology, pharmacology and toxicology. however, serum supplementation has extensively been used to improve the viahility of tissue culture. This study was undertaken to evaluate the effect of serum on the histologic changes ohserved during the organ culture of the normal human skin. The general architecture of the skin was well maintained for 6 days with or without seru. After then, fetal calf serum or autologous human serum was found to enhance the viability of the epidermis. A confluent layer of necrotic spinous ceils was ovserved earlier and more widespread without serum. The addition of serum had an impressive effect on epibolization. In the absenee of serum, the formation of the epibolus was not only minimal, but also, susceptible to degeneration, and no epibolus remained at 10 days rif incubation. No difference can be found between fetal calf serm and autologous human serum in the formation of the epibolus. There was no favorable effect of serum on the formation of new stratum corneum. The thickness of new straturn corneum increased in parallel with the number of parakeratatic cells, increasing most rapidly between 6 and 8 days of incubation. Parakeratosis was more prominent in the presence of serurn. (Kor J Dermatol 28(2): 130 135, 1990)
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